• Horticultural Science & Technology
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• v.33 no.1
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• pp.83-92
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• 2015

xBrassicoraphanus line BB#5, a new synthetic intergeneric hybrid between Brassica rapa L. ssp. pekinensis and Raphanus sativus L. var. rafiphera induced by N-methyl-N-nitroso-urethane mutagenesis in microspore culture, shows high seed fertility and morphological uniformity. Dual-color fluorescence in situ hybridization (FISH) using 5S and 45S rDNA probes and genomic in situ hybridization (GISH) using B. rapa genomic DNA probe were carried out to analyze the chromosome composition and the meiosis pairing pattern compared to its parental lines. The somatic chromosome complement is 2n = 38, which consists of 17 metacentric and two submetacentric chromosomes with lengths of 2.18 to $5.01{\mu}m$. FISH karyotype analysis showed five and eight pairs of 5S and 45S rDNA loci. GISH meiosis pairing analysis showed that 19 complete bivalents were most frequent and accounted for 42% of the 100 pollen mother cells examined. Based on chromosome number, size, morphology, rDNA distribution, and meiosis pairing pattern, both parental genomes of B. rapa and R. sativus appear to exist in xBrassicoraphanus line BB#5, demonstrating its genome integrity. Such stable chromosome constitutions and meiotic pairing patterns in somatic and meiotic cells are very rare in natural and synthetic intergeneric hybrids. Chromosomal studies and genetic and phenotypic changes in allopolyploids a re discussed. The results p resented h erein will b e usef ul f or f urther g enomic s tudy o f xBrassicoraphanus lines and their improvement as promising new breeding varieties.

• Journal of Electrical Engineering and Technology
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• v.13 no.6
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• pp.2545-2552
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• 2018

$SF_6$ has been widely used in high voltage power equipment, such as gas insulated switchgear (GIS) and gas insulated transmission line (GIL), because of its excellent insulation and arc extinguishing performance. However, $SF_6$ faces two environmental problems: greenhouse effect and high liquefaction temperature. Therefore, to find the $SF_6$ substitute gases has become a research hotspot in recent years. In this paper, the liquefaction characteristics of $SF_6$ substitute gases were studied. Peng-Robinson equation of state with the van der Waals mixing rule (PR-vdW model) was used to calculate the dew point temperature of the binary gas mixtures, with $SF_6$, $C_3F_8$, $c-C_4F_8$, $CF_3I$ or $C_4F_7N$ as the insulating gas and $N_2$ or $CO_2$ as the buffer gas. The sequence of the dew point temperatures of the binary gas mixtures under the same pressure and composition ratio was obtained. $SF_6/N_2$ < $SF_6/CO_2$ < $C_3F_8/N_2$ < $C_3F_8/CO_2$ < $CF_3I/N_2$ < $CF_3I/CO_2$ < $c-C_4F_8/N_2$ < $C_4F_7N/N_2$ < $c-C_4F_8/CO_2$ < $C_4F_7N/CO_2$. $SF_6/N_2$ gas mixture showed the best temperature adaptability and $C_4F_7N/CO_2$ gas mixture showed the worst temperature adaptability. Furthermore, the dew point temperatures of the $SF_6$ substitute gases at different pressures and the upper limits of the insulating gas mole fraction at $-30^{\circ}C$, $-20^{\circ}C$ and $-10^{\circ}C$ were obtained. The results would supply sufficient data support for GIS/GIL operators and researchers.

• Journal of the Korean Institute of Electrical and Electronic Material Engineers
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• v.15 no.1
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• pp.85-92
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• 2002

In this study, we analyzed and experimented micro-strip line inductors and capacitors for a SAW duplexer, an important devise for mobile communication. For SAW duplexers, micro-strip line lumped elements must have small impedance values, below several tens of nH or several tens of pF, and a small area pattern. In this study, we performed theoretical analysis of flat line type, meander line type, and spiral line type inductors and interdigital capacitors on a LiTaO$_3$ Piezo-crystal. We proposed a measurement method to evaluate small values of lumped elements accurately with network analyzer. In experiments, we confirmed validity of the theoretical analysis method through fabrication and characterization of micro-strip line lumped elements. The analysis method in this paper can be applied to SAW duplexers well as other microwave devices.

• Asian-Australasian Journal of Animal Sciences
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• v.22 no.4
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• pp.492-499
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• 2009

A Silkie Bantam embryo fibroblast line (named SBF59 line) was successfully established by using direct explant culture and cryopreservation techniques. Cell morphology, viability, dynamic growth and contamination were tested and the karyotype and levels of isoenzymes of lactic dehydrogenase and malic dehydrogenase were analyzed. Four kinds of fluorescent protein extrogenes, including $pEGFP-N_3$, $pECFP-N_1$, $pEYFP-N_1$ and $pDsRed1-N_1$ were transfected into the cells. The results showed that the cells were healthy and possessed a fibrous structure without a change in morphology. The average viability of the cells was 96% before freezing and 90.5% after thawing. The growth curve appeared as typical "S" shape and the cell growth passed through a detention phase, a logarithmic phase and a platform phase; the estimated population doubling time (PDT) was 38.5 h; assays for the presence of bacteria, fungi, viruses and mycoplasmas were negative; the cell line showed no cross contamination when assessed by isoenzyme analysis; the chromosome number was 2n = 78 on more than 88% of occasions; four kinds of fluorescent protein extro-genes appeared to be expressed effectively with a high transfection efficiency between 18.3% and 42.3%. The cell line met the required quality control standard. It not only preserves the genetic resources of the important Silkie Bantam at the cellular level but also provides valuable materials for genomic, post-genomic, somatic cell cloning research and other applications.

• Bulletin of the Korean Mathematical Society
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• v.52 no.6
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• pp.2047-2069
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• 2015

The coset diagrams are composed of fragments, and the fragments are further composed of circuits at a certain common point. A condition for the existence of a certain fragment ${\gamma}$ of a coset diagram in a coset diagram is a polynomial f in ${\mathbb{Z}}$[z]. In this paper, we answer the question: how many polynomials are obtained from the fragments, evolved by joining the circuits (n, n) and (m, m), where n < m, at all points.

• Journal of the Korean Society for Precision Engineering
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• v.16 no.12
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• pp.143-151
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• 1999

The shape of surface micro-crack is very irregular due to nonhomogeneous microstructure but is very important in respect to qualitative estimation of fatigue life. Fractal geomety can quantify the shape of surface mciro-crack. Fractal dimension is measured for surface micro-cracks with coast line and box counting method and estimates cycle ration in Al 2024-T3. The average fractal dimension $D_{favg}$ of surface micro-cracks has 3-parameter weibull distribution and location parameter is nearly constant but shape parameter decreases as cycle ration increases. The fractal dimension by coast line method is measured for individual surface micro-crack but the fractal dimension by box countin method is measured for all the surface micro-cracks under sampling area. Therefore, This paper shows fractal dimension $D_{fb}$ can predict cycle ratio $N/N_f$ more convenient than fractal dimension $D_{favg}$.

• The Journal of the Korea institute of electronic communication sciences
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• v.9 no.10
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• pp.1125-1130
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• 2014

The quantity and quality of contents containing information are sharply increasing with the rising network speed. In line with this rapid growth of information volume, a new communication protocol using the chaotic signal that can protect contents in communication is proposed as follows. The chaos system has the characteristic of unpredictability due to the sensitive initial values and the similarity of the signals with noise. We configured two chaos systems $F(X_n,Y_n)$ and $G(A_n,B_n)$ that have such characteristics and designed a data communication method using as encryption channel the same chaos signals generated by synchronizing the chaos system G with the F signals. The proposed method was verified with the encryption and decryption of images. The proposed method is different from the existing encrypted communication methods and is expected to lay the foundation for future studies in related areas.is an example of ABSTRACT format.

• 한국어정보학회:학술대회논문집
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• 2016.10a
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• pp.321-323
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• 2016

최근 연구에서 기계학습 중 지도학습 방법으로 개체명 인식을 하고 있다. 그러나 지도 학습 방법은 데이터를 만드는 비용과 시간이 많이 필요로 한다. 본 연구에서는 주석 된 말뭉치를 사용하여 지도 학습 방법을 사용 한다. 의생명 개체명 인식은 Protein, RNA, DNA, Cell type, Cell line 등을 포함한 텍스트 처리에 중요한 기초 작업입니다. 그리고 의생명 지식 검색에서 가장 기본과 핵심 작업 중 하나이다. 본 연구에서는 순환형 신경망과 워드 임베딩을 자질로 사용한 조건적 임의 필드에 대한 성능을 비교한다. 조건적 임의 필드에 N_Gram만을 자질로 사용한 것을 기준점으로 설정 하였고, 기준점의 결과는 70.09% F1 Score이다. RNN의 jordan type은 60.75% F1 Score, elman type은 58.80% F1 Score의 성능을 보여준다. 조건적 임의 필드에 CCA, GLOVE, WORD2VEC을 사용 한 결과는 각각 72.73% F1 Score, 72.74% F1 Score, 72.82% F1 Score의 성능을 얻을 수 있다.

• Journal of the Korean Society of Clothing and Textiles
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• v.23 no.3
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• pp.353-360
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• 1999

An investigation made of the variations of angle of bias on the top of the sleeve cap curve line and calculated easing contraction ratio by capheights(A ; a,h$\times$5,/6) B: A, H/4 +4cm C:A.H/3 D: A.H/ 4+3cm E:AH/4+2cm, F: A,H/4+1cm, G: A,H/4, H:A,H/6, I:A,H/8) and the efects of easing contraction on the cap curve lines of sleeve A, D, G by easing stitch density with the gathering foot: sewing condition-lockstitch industrial machine stitch density(N1.0 ; 38stitches/3cm N1.5: 26stitches/3cm, N2.0 ; 19stitches/3cm, N2.5 ; 14stitches/ 3cm) The results obtained were as follows; 1) The variations of the angle of bias on the top of the sleeve cap curve line by cap heights can be done according to the angle balance (front; $\alpha$-$\beta$ back ; $\alpha$'- $\beta$') between the angle (front ;$\alpha$, $\beta$, back ; $\alpha$'- $\beta$') of bias of the two base-lines. 2) The higher cap height the more higher the calculated easing contraction ratio. 3) The lower the stitch density the higher easing contraction ratio. 4) The effects of easing contraction was that sleeve G was more than sleeve A, D.

• Journal of Korean Society of Forest Science
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• v.101 no.2
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• pp.291-296
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• 2012

Triploids are a useful tool for biomass production and molecular breeding of trees with a long life span. Triploids of the poplar 'Hyunsasi' (Populus alba ${\times}$ P. glandulosa) have been developed by crossing between female diploids and a male tetraploid. The tetraploid was developed around the 1970s at Korea Forest Research Institute by colchicine-induced chromosome doubling. Seedlings of the $F_1$ generation were analyzed using flow cytometry to verify their ploidy status. The mean relative fluorescence index of 3 F1 poplars, labeled as Line- 1, Line-17, Line-18, were approximately 1.5 times higher than those of diploid poplars, and the results clearly indicated that they were triploids. The phenotype of the F1 poplars included larger leaves and thicker stem than diploids, and abnormal leaf morphology, especially in the triploid 'Line-18'. Three triploid lines developed roots more slowly and had less roots than diploid. However, 3 poplar cytotypes (2x, Line-1, Line-17) rooted within 10 days on MS medium. In contrast, compared with the 3 cytotypes, the Line-18 showed about 80% and 70% in the rooting rate and the number of roots. The triploid poplars could be directly utilized for biomass production and with their sterility, they could serve as basic material for genetic transformation. In addition, flow cytometric analysis proved to be an effective and reliable method for screening forest trees for their ploidy level.