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Selection of a Triploid Poplar by Flow Cytometric Analysis and Growth Characteristics of its in vitro Grown Plants  

Bae, Eun-Kyung (Division of Forest Biotechnology, Korea Forest Research Institute)
Lee, Hyoshin (Division of Forest Biotechnology, Korea Forest Research Institute)
Lee, Jae-Soon (Division of Forest Biotechnology, Korea Forest Research Institute)
Noh, Eun-Woon (Division of Forest Biotechnology, Korea Forest Research Institute)
Publication Information
Journal of Korean Society of Forest Science / v.101, no.2, 2012 , pp. 291-296 More about this Journal
Abstract
Triploids are a useful tool for biomass production and molecular breeding of trees with a long life span. Triploids of the poplar 'Hyunsasi' (Populus alba ${\times}$ P. glandulosa) have been developed by crossing between female diploids and a male tetraploid. The tetraploid was developed around the 1970s at Korea Forest Research Institute by colchicine-induced chromosome doubling. Seedlings of the $F_1$ generation were analyzed using flow cytometry to verify their ploidy status. The mean relative fluorescence index of 3 F1 poplars, labeled as Line- 1, Line-17, Line-18, were approximately 1.5 times higher than those of diploid poplars, and the results clearly indicated that they were triploids. The phenotype of the F1 poplars included larger leaves and thicker stem than diploids, and abnormal leaf morphology, especially in the triploid 'Line-18'. Three triploid lines developed roots more slowly and had less roots than diploid. However, 3 poplar cytotypes (2x, Line-1, Line-17) rooted within 10 days on MS medium. In contrast, compared with the 3 cytotypes, the Line-18 showed about 80% and 70% in the rooting rate and the number of roots. The triploid poplars could be directly utilized for biomass production and with their sterility, they could serve as basic material for genetic transformation. In addition, flow cytometric analysis proved to be an effective and reliable method for screening forest trees for their ploidy level.
Keywords
Populus alba ${\times}$ P. glandulosa; diploid; triploid; flow cytometry; growth characteristics;
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