• Environmental Engineering Research
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• v.24 no.2
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• pp.254-262
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• 2019

The impact of free cyanide ($CN^-$) and thiocyanate ($SCN^-$) on the $CN^-$ (CDO) and $SCN^-$ degraders (TDO) to nitrify and denitrify aerobically was evaluated under alkaline conditions. The CDO's were able to nitrify under cyanogenic conditions, achieving $NH_4{^+}-N$ removal rates above 1.66 mg $NH_4{^+}-N.L^{-1}.h^{-1}$, except when $CN^-$ and $SCN^-$ loading was 15 mg $CN^-/L$ and 50 mg $SCN^-.L^{-1}$, respectively, which slightly inhibited nitrification. The TDO's were able to achieve a nitrification rate of 1.59 mg $NH_4{^+}-N.L^{-1}.h^{-1}$ in the absence of both $CN^-$ and $SCN^-$, while the presence of $CN^-$ and $SCN^-$ was inhibitory, with a nitrification rates of 1.14 mg $NH_4{^+}-N.L^{-1}.h^{-1}$. The CDO's and TDO's were able to denitrify aerobically, with the CDO's obtaining $NO_3{^-}-N$ removal rates above 0.67 mg $NO_3{^-}-N.L^{-1}.h^{-1}$, irrespective of the tested $CN^-$ and $SCN^-$ concentration range. Denitrification by the TDO's was inhibited by $CN^-$, achieving a removal rate of 0.46 mg $NO_3{^-}-N.L^{-1}.h^{-1}$ and 0.22 mg $NO_3{^-}-N.L^{-1}.h^{-1}$ when $CN^-$ concentration was 10 and 15 mg $CN^-.L^{-1}$, respectively. However, when the CDO's and TDO's were co-cultured, the nitrification and aerobic denitrification removal rates were 1.78 mg $NH_4{^+}-N.L^{-1}.h^{-1}$ and 0.63 mg $NO_3{^-}-N.L^{-1}.h^{-1}$ irrespective of $CN^-$ and $SCN^-$ concentrations.

• Journal of The Korean Society of Grassland and Forage Science
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• v.5 no.3
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• pp.187-194
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• 1985

This field experiment was carried out to determine the effects of thirteen different fertilizer levels of nitrogen(N), phosphorus($P_2O_5$) and potassium($K_2O$) on the content of crude protein, crude fiber, mineral constituents of product and tree growth forest pasture with 40-50% shading. The experiment was arranged as a randomized block design and performed in the suburban forest of Suweon in 1984. The results obtained are summarized as follows: 1. Crude protein content and total protein yield were higher in the plot of 28 and 42kg $N/_{10a}$, regardless of $P_2O_5\;and\;K_2O$ level, while those were the lowest in zero fertilizer and N-zero fertilizer plots. 2. The contents of lignin and silica were significantly low in the high N fertilizer level, and the contents of NDF, ADF, cellulose and hemicellulose were not affected by different fertilizer levels. However, the content of crude fiber tended to be low with high N, regardless of $P_2O_5\;and\;K_2O$. 3. The contents of N,K and $SiO_2$ of grasses were influenced by different fertilizer levels. However, those of P,Ca,Ma and Na showed little differences. 4. The recovery percentage of NPK was higher in the plot of standard fertilizer level with 28-20-24 kg/10a, and higher recovery percentage was observed in $K_2O$, followed by N and $P_2O_5$ in that order. 5. The growth of tree was increased as the level of N fertilizer was increased, but no such trend was noted by $P_2O_5\;and\;K_2O$ levels. 6. Crude protein, crude fiber, some mineral contituents of grasses, and growth of tree were influenced by N level, regardless of $P_2O_5\;and\;K_2O$. And the optimum fertilizer level of $N-P_2O_5-K_2O$ seemed to be 28-20-24 kg/10a for the production of grasses with higher quality and more yield in the forest.

• Korean Journal of Soil Science and Fertilizer
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• v.32 no.2
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• pp.123-131
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• 1999

To get the basic information for the improvement of the optimum levels of upland soil fertility and fertilizer application, the soil samples in two hundred fifteen site were collected from the major condiment vegetable cultivation areas such as red pepper, garlic and onion fields. Physico-chemical properties of the soil samples were investigated. Soil texture distribution of soil samples in red pepper, garlic and onion cultivation areas was in order of loam (L), sandy loam (SL), silt loam (SiL) and clay loam (CL) (35.4, 31.6, 14.9 and 7.0%, respectively). The average pH of soil in garlic and onion cultivation areas were over pH 6.0, whereas in red pepper was under pH 5.5. The frequency distribution of soil pH in total sampling sites were 58.7% in under pH 6.0 and 21.4% in below pH 5.0, in contrast to 10.3% in over pH 7.0. The organic matter contents were in the range of $20{\sim}30g\;kg^{-1}$, and the onion field soils was a little higher than those in red pepper or garlic. The available phosphate contents were in the range of $719{\sim}746mg\;kg^{-1}$ and were not different among in red pepper, garlic and onion. The frequency distribution of available phosphate in total sampling sites were found 62.8% of above $600mg\;kg^{-1}$, which was over the standard level for upland soil improvement, and then 22.3% was exceeded $1,000mg\;kg^{-1}$, especially. In the exchangeable cations, the K and Ca contents in garlic (1.27 and $9.11cmol\;kg^{-1}$) and onion (1.20 and $8.39cmol\;kg^{-1}$) were higher than in red pepper (0.96 and $5.87cmol\;kg^{-1}$), respectively. The Mg contents in garlic field soils ($2.17cmol\;kg^{-1}$) were higher than those in red pepper and onion (1.51 and $1.80cmol\;kg^{-1}$). This exchangeable K, Ca and Mg contents were higher than the standard level for upland soil improvement. The contents of microelement were ranged in $54.3{\sim}60.1mg\;kg^{-1}$ for Fe, $31.3{\sim}42.3mg\;kg^{-1}$ for Mn, $1.7{\sim}2.3mg\;kg^{-1}$ for Cu and $4.8{\sim}5.5mg\;kg^{-1}$ for Zn, respectively.

• Korean Journal of Environmental Agriculture
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• v.32 no.4
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• pp.279-286
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• 2013

BACKGROUND: Soil incorporation of green manure crop(GMC) is one of the methods for reducing continuous cropping injury and increasing yield of carrot. The purpose of this study was to evaluate the effects of green manure crops on growth and yield of carrot for reduction of continuous cropping injury of carrot through crop rotation. METHODS AND RESULTS: To reduce the injury by continuous cropping system(CCS) of carrot cultivation, GMCs such as crotalaria and sudangrass were applied, which GMC was sowed in latter-June and returned to soil in latter-October. Nutrient contributions of N, $P_2O_5$, $K_2O$, CaO, and MgO in crotalaria were 8.3, 7.5, 4.4, 7.8, and 2.1 kg/10a, respectively. Nutrient contributions of N, $P_2O_5$, $K_2O$, CaO, and MgO in sudangrass were 8.4, 8.6, 26.8, 0.3, and 2.7 kg/10a, respectively. After incorporation of GMCs into soil, bulk density in soil with GMCs was lower than that in soil without GMCs(control). In soil after incorporation of GMCs, pH was not different in all treatment conditions, and ranged from 6.37~6.64. EC in soil after incorporation of GMCs was lower than that in soil without GMCs. The OM, T-N, and avail. $P_2O_5$ contents in soil with GMCs were higher than those in soil without GMCs. The growth and yields were increased as 39.2%(6,226 kg/10a) in the rotational cropping system(RCS) as compared to continuous cropping system(control and without NPK) of 4,473 kg/10a. Crotalaria cultivation were the most effective crop for reducing the injury of continuous cropping of carrot. CONCLUSION(S): This study suggest that the RCS using GMCs showed lower disease outbreak density in soil for carrot cultivation as compared to CCS without GMCs. Especially, the GMCs good effect for reduction of continuous cropping injury of carrot.

• The Journal of Korean Medicine
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• v.30 no.2
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• pp.63-78
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• 2009

Objective: The purpose of experimental study was to prove the effects of Boyangmakseong-bang (BYMSB) treatment on cBSA-induced in a MN mouse model. Methods: We divided mice into 4 groups. The Normal group had no treatment. We used cBSA and induced MN mouse model to the other 3 groups. The Control group was treated with cBSA (9mg/kg i.p) only. The second group, named 'BY-250', was treated with cBSA (9mg/kg i.p) and BYMSB extract (250mg/kg, p.o). The third group, named 'BY-500', was treated with cBSA (9mg/kg i.p) and BYMSB extract (500mg/kg, p.o). After cBSA and BYMSB extract treatment for 4 weeks, the increase in percentage of body weight, proteinuria, serum albumin, total cholesterol, creatinine and BUN of all groups were measured. The CD3+, CD19+, CD4+, CD8+ cell levels of spleen of all groups were analyzed. IgA, IgG, IgM, IL-$1{\beta}$, TNF-${\alpha}$, IL-6 and IFN-${\gamma}$ levels of all groups were gauged. H&E staining, immunofluorescence staining and electron microscopy of kidney were observed. Results: BYMSB showed significant decrease in the 24hrs proteinuria, serum total cholesterol, serum IgG levels and BUN levels, and showed significant increase in the serum albumin levels compared with the control group. BYMSB showed increase in the increasing percentage of body weight and IFN-${\gamma}$ levels compared with the control. BYMSB showed decrease in the CD3+ T cells, CD4+ Th cells, IL-$1{\beta}$, TNF-${\alpha}$ and IL-6 levels, but did not show significant change compared with the control. BYMSB showed considerable decrease in the thickening of the GBM on H&E staining, deposition of IgG on immunofluorescence staining and deposition of electron-density on electron microscopy of kidney compared with the control. Conclusions: According to the above results, it is suggested that BYMSB decreases the symptoms of MN induced by cBSA in a mouse model. Therefore BYMSB seems to be applicable to MN in clinical practice.

• The Korean Journal of Pain
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• v.8 no.2
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• pp.257-265
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• 1995

Patient-Controlled Analgesia (PCA) has been widely used for postoperative pain relief. Meperidine is useful for PCA and has efficient analgesia, rapid onset, and low incidence of adverse effect. To compare the analgesic effect, total dose and hourly dose, side effect and neonatal status of breast feeding with meperidine via intravenous or epidural PCA for 48 hours after Cesarean Section, 40 parturient women undergoing elective Cesarean Section were randomly divided into two groups. Each respective group of 20 parturient women received meperidine via one of the intravenous PCA after general anesthesia with enflurane (IVPCA group) and the epidural PCA after general anesthesia with enflurane (IVPCA group) and the epidural PCA after epidural block with 2% lidocaine 20ml combined with general anesthesia with only $N_2O$ and $O_2$ (EpiPCA group) when they first complained of pain in recovery room. Following the administration of analgesic initial dose, parturient women of IVPCA group were allowed intravenous meperidine 10 mg every 8 minutes when they felt pain. The EpiPCA group received additional bolus dose of meperidine 2 mg and bupivacaine 0.7 mg were administered every 8 minutes as requested the patients with hourly continuous infusion of meperidine 4 mg and bupivacaine 1.4 mg. Data was collected during the 48 hours observation period including visual analog scale (VAS) pain scores, total meperidine dose, hourly dose during 48 hours and each time interval, incidence of adverse effect, satisfaction, and neonatal status with breast feeding. VAS pain scores of analgesic effect in EpiPCA group was significantly lower than in IVPCA group at 2 hours after the initial pain after Cesarean Section. Total dose and hourly dose of meperidine significantly reduced in EpiPCA group. Hourly dose of meperidine at each time interval significantly reduced during first 6 hours and from 12 hours to 24 hours in EpiPCA group. The side effects in IVPCA group were mainly sedation, nausea, and local irritation of skin. And EpiPCA group experienced numbness and itching. The degree of satisfaction of parturient women was 88.2 % in IVPCA group and 85.7 % in EpiPCA group. We did not observe any sedation, abnormal behavior, or seizure like activity in any neonates of breast feeding. From the above results we conclude that epidural PCA was more efficiently analgesic, less sedative, and consumptional, and safer for neonate than intravenous PCA, and could be an alternative method to intravenous PCA.

• The Korean Journal of Ecology
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• v.19 no.1
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• pp.21-29
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• 1996

Variation in triacylglycerols(TGs) and fatty acids in Fucus serratus was analyzed for a period of one year. TGs were more concentrated during the summer(2.8mg/g dw)and autumn(2.6mg/g dw) than during the spring (0,7mg/g dw)and winter (0.5mg/g dw). The dominant fatty acides in total liqid were palmitic acid ($C_{16:0}$, 24.1%), oleic acid (($C_{18:1}$, 22.4%) and arachidonic acid (($C_{20:4}$, 14.4%) but the dominant ones in TG were $C_{16:0}$(22.8%), $C_{18:1}$(36.4%) and $C_{18:2}$(linoleic acid, 16.4%). The levels of $C_16$ fatty acids were high in winter while $C_18$ in summer and autumn. The polyunsaturated fatty acids (UFAs) were more abundant in the $C_20$ series, while the UFAs of the $C_16$ were low. Especially, the amount of arachidonic acid ($C_{20:4}$, 14.4% of total fatty acids (TFA) was more abundant than that of eicosapentaenoic acid ($C_{20:5}$, 10.4% of TFA). The amount of $C_{20:4}$ and $C_{20:5}$,in TG was 9.2% and 4.8%, respectively. These UFAs in total lipid were thus higher than TG. Therefore, the synthesis of TG and fatty acid was stimulated by the alternation of emersion and submersion of thalli from sea water and eco-physiological conditions during summer: high temperature and light, and low concentration of nitrogen.

• Korean Journal of Soil Science and Fertilizer
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• v.50 no.3
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• pp.150-161
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• 2017

This study was conducted with the data of monitoring on soil chemical properties of rice paddy soils in Gyeongbuk Province. The selected soil chemical properties were analyzed every 4 year from 1999 to 2015. The soil pH measured in 2015 was higher than pH 6.0, which was 0.3-0.4 pH unit higher than data until 2007 survey year. The mean content of organic matter was greater than $24g\;kg^{-1}$ since 2003, but 35% of soil samples remained below the recommended level ($20-30g\;kg^{-1}$) in 2015. The mean concentration of available phosphate was maintained at $40mg\;kg^{-1}$ higher than the upper recommendation level ($80-120mg\;kg^{-1}$), and more than 40% of paddy soils tested were found to have less than the recommendation level during the survey period. The exchangeable K concentration ranged from 0.25 to $0.39cmol_c\;kg^{-1}$. Exchangeable Ca showed an average at the optimum range ($5.0-6.0cmol_c\;kg^{-1}$) during the monitoring period. Exchangeable Mg decreased linearly ($0.02cmol_c\;kg^{-1}\;year^{-1}$) from $1.55cmol_c\;kg^{-1}$ as of 1999 to below the lower level of the recommendation range ($1.5-2.0cmol_c\;kg^{-1}$). The amount of available $SiO_2$ was increased significantly from 2011 to over the recommendation level (${\geq}157mg\;kg^{-1}$). It was revealed that the soil chemical properties of rice paddy fields was influenced by topology, soil texture, type and region as result of principal component analysis or cluster analysis. Therefore, an assessment on chemical properties of rice paddy soils should be performed to consider various soil physical conditions and agronomic practices such as fertilization, cropping system, and so on. Because of the high variability of nutrient levels across Gyeongbuk Province, nutrient management based on soil fertility test is required by respective farm land unit.

• Journal of Veterinary Clinics
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• v.17 no.2
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• pp.480-484
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• 2000

A 10 years old female Poodle weighing 4.38 kg and a 10 years old female Maltese weighing 4.33kg were referred to veterinary leaching hospital of college of veterinary medicine, Konkuk uni-versity because of hematuria and severa anorexia. Preoperative hematological and srum chemical values revealed that counts of WBC were $78.4{\times}10^3$,/TEX>/ul in the Poodle and $21.6{\times}10^3$in the Maltese, concentrations of BUN 105 and 13 mg/dl, concentrations of creatinine 4.0 and 1.4 mg/dl, concentration of ALKP 542 U/L in the Poodle and concentration of ALF 37 U/L in the Maltese, respectively. Radiographic findings were observed radiopaque bilateral nephroliths in the Poodle and right renal cal- culus in the Maltese. Ultrasonographic findings were showed hyperechoic mass with severe acoustic shadowing, which were ]ocated in the center of both kidneys parenchyma in the Poodle and in right kidney in the Maltese. Bisectional nephrotomy in right kidney for removal of calculus, which had ,sin- gle stone and more large than splitted stones in lets kidney in the Poodle, was performed under isnf- lurane anesthesia in 100% $O_2$. The time from temporary occlusion of blood flow to suture of incisioned kidney after removal of renal calculi was elapsed 12 minutes. During operation and until 24hrs after operation was infusioned mannitol and tranexanic acid solution. There were normal urination with yellow color in the Poodle at 3 days after operation and in the Maltese at 1 day and good appetite after 7 days and 1 day after operation. At 7 days and 9 days after operation, hematolgoical and seam chemical values repealed that counts of WBC were $29.8{\times}10^3/u1 in the Poodle and 13.1 {\itmes}10^3$/ul in the Mal- tese, concentrations of BUN 13 and 10 mg/dl. concentrations of creatinine 1.3 and 1.1 mg/dl, con-centrations of ALT 34 and 97U/L, concentraion of AST 10 U/L in the Poodle, respectively, By ultra-sonographic view there was not observed hyperechoic mass and radippaque calculus in operated kid-ney in radiographic view.

• YAKHAK HOEJI
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• v.30 no.1
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• pp.31-41
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• 1986

Sarcolemmal membrane fraction from canine ventricle was isolated from the discarded pellet after the first homogenization in the isolation procedure of sarcoplasmic reticulum (Method 1) and the protein yield, purity, and sidedness of this preparation were compared to those of sarcolemmal fraction prepared by method of Lee et al. (Method 2) and a slight modification of original protocol of Jones et al. (Method 3). Method 1 differed from Method 2 essentially only in that vigorous homogenization was carried out by omnimixer and homogenization medium containing 30mM Tris-maleate was used in the first step. The sarcolemmal fraction was enriched from 45 to 50 and 29-fold in [$^3H$] ouabain, [$^3H$] DHA, [$^3H$] QNB binding and $Na^+$, $K^+$-ATPase activity, respectively, compared to homogenate. Total $Na^+$, $K^+$-ATPase activity of highly sarcolemma enriched fraction was 144.6$\pm$16.4$\mu\textrm{mol}$ Pi/mg protein/hr, which was about 85%, of total ATPase activity, and the yield of the preparation was 15.7 mg protein per 100g of starting ventricular tissue. The sarcolemmal preparation supported $^{45}Ca^{2+}$-uptake in the presence of ATP but this uptake was not dependent on oxalate. Sarcolemmal $Na^+$, $K^+$-ATPase activity and detectable [$^3H$] ouabain binding were increased about 32% and 35%, respectively, by pretreatment of sarcolemmal fraction with optimal concentration of sodium dodecylsulfate (0.3-0.4mg/mg protein), suggesting that this preparation contained about 24% of sealed rightside-out vesicles, 26% of sealed inside-out vesicles, and 5001o of freely permeable (leaky) form. This procedure showed the highest protein yield and leaky population, compared to Method 2 and 3. On the other hand, sarcolemmal fraction prepared by Method 2 and 3 showed low value in protein yield but comtained high population of inside-out (46%) and rightside-out (49%) vesicles, respectively, compared to present procedure (Method 1). The results indicate that vigorous homogenization decreases the population of sealed sarcolemmal vesicles but increases the sarcolemmal protein yield per gram tissue and that this procedure is available for further purification of sarcolemmal fraction and for the receptor binding study of sarcolemma.