• Title/Summary/Keyword: $L_1-B_4$

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Efficacy of Mixture of Lactic Acid Bacteria (LAB) and Bifidobacteria Supplement in the Management of Constipation; Demonstration of Functionality in Animal and Clinical Trials (한국형 Bifidobacterium longum SPM1205 혼합제제의 변비 개선 효과)

  • Kim, Jung-Rae;Lee, Do-Kyung;Baek, Eun-Hye;An, Hyang-Mi;Yang, Hwan-Jin;Kim, Mi-Jin;Choi, Kyung-Soon;Yun, Mi-Eun;Jung, Yi-Jung;Oh, Pok-Ja;Chung, Myung-Jun;Ha, Nam-Joo
    • Korean Journal of Microbiology
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    • v.46 no.1
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    • pp.52-62
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    • 2010
  • The aim of this study was to evaluated the efficacy of mixture of Lactic Acid Bacteria (LAB) and bifidobacteria supplement, which are contained with Lactobacillus acidophilus, Bifidobacterium longum SPM1205, and Pediococcus pentosaceus for the management of constipation in animal model and clinical trials. 5 ICR mice and 4 female constipation subjects were orally taken mixture of LAB and bifidobacteria for 2 weeks. We investigated the number of fecal LAB and harmful enzymes activities before and after mixture of LAB and bifidobacteria application. As a result, fecal LAB count was increased and harmful enzymes activities of intestinal microflora were generally decreased after mixture of LAB and bifidobacteria application. Also, 61 female subjects were randomly assigned to receive either mixture of LAB and bifidobacteria or lactose and were taken three times a day for 2 weeks. Then, we analyzed mixture of LAB and bifidobacteria effect through the questionnaires. Daily consumption of this mixture of LAB and bifidobacteria improved the constipation in constipation group (56.3%) compared with lactose application group (26.7%). Furthermore, after mixture of LAB and bifidobacteria treatment, frequency of hard stool decreased from 0.22 to 0.03. These results indicated that mixture of LAB and bifidobacteria application is effective to improve the constipation.

Quality Characteristics of Onion Jangaji during Aging (숙성조건에 따른 양파장아찌의 품질특성)

  • Kang, Nan-Suk;Kim, Jun-Han;Kim, Jong-Kuk
    • Food Science and Preservation
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    • v.15 no.6
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    • pp.796-803
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    • 2008
  • To aid in manufacturing, this study investigated quality characteristics of onion Jangaji during maturation at 0C, 10C, 20C and room temperature. [Please check that the rewriting of the previous sentence retains the intended meaning]The $^{\circ}Brix$ of onion Jangaji and the maturation solution were 1516% at the end of the maturation process. The salinity of onion Jangaji and the solution increased, and the pH declined to 3.934.31 during processing. The L, a and b color values of onion Jangaji and the solution changed slightly. The total acidity of onion Jangaji and the solution rose slowly. The texture (hardness, fracturability, adhesiveness, cohesiveness, springiness, gumminess and chewiness) of onion Jangaji was determined using a texture analyzer, and varied with storage temperature. In the sensory evaluation of onion Jangaji, low temperature storage gave higher acceptability ratings than room temperature with respect to taste, color, flavor, texture and overall acceptability.

Effect of chloride ions on the catalytic properties of human pancreatic α-amylase isozyme produced in Pichia pastoris (Pichia pastoris에서 생산된 인체 췌장 α-아밀레이스 동질효소의 촉매활성에 대한 염소이온의 영향)

  • Kim, Min-Gyu;Kim, Young-Wan
    • Korean Journal of Food Science and Technology
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    • v.48 no.4
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    • pp.341-346
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    • 2016
  • The AMY2B gene, encoding human pancreatic ${\alpha}$-amylase isozyme (HPA II), was expressed in Pichia pastoris, and the effects of chloride ions on HPA II activity toward starch substrates were investigated. As seen with chloride ion-dependent ${\alpha}$-amylases-including HPA I, the isozyme of HPA II-chloride ions increased enzyme activity and shifted the optimal pH to an alkaline pH. The activity enhancement by chloride was more significant at pH 8 than that at pH 6, suggesting that the protonation state of the general acid/base catalyst of HPA II was important for the hydrolysis of starches at an alkaline pH because of the increase in its $pK_a$ by chloride ions. The turnover values for cereal starches as the substrates markedly increased in the presence of chloride by up to 7.2-fold, whereas that for soluble starch increased by only 1.7-fold. Chloride inhibited substrate hydrolysis at high substrate concentrations, with $K_i$ values ranging from 6 to 15 mg/mL.

Study on quality characteristics of Mandupi added with Ligularia fischeri powder (곰취 분말을 첨가한 만두피의 품질 특성)

  • Park, Bock-Hee;Kim, Se-Jin;Cho, Hee-Sook
    • Food Science and Preservation
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    • v.22 no.4
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    • pp.475-481
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    • 2015
  • The purpose of this study was to investigate the effect of Ligularia fischeri powder on the functional and sensory characteristics of Mandupi. Various Mandupi samples were prepared with wheat flour by addition of different amounts of Ligularia fischeri powder, and instrumental characteristics and sensory evaluations were then investigated. Based on amylograph data, the composite of Ligularia fischeri powder and wheat flour samples increased its gelatinization temperatures depending on increasing of Ligularia fischeri powder content. However, viscosities at $95^{\circ}C$ and after 15 min, and maximum viscosity were reduced. In terms of Hunter's color values, L and a values decreased, while b value increased with the increase of Ligularia fischeri powder content. In addition, weight, volume and turbidity of Mandupis prepared with Ligularia fischeri powder were significantly greater than those of control. In terms of textural characteristics, springiness, chewiness and brittleness increased whereas adhesiveness decreased with the addition of Ligularia fischeri powder. Sensory evaluations showed that the overall preference of Mandupi with the addition of 3% Ligularia fischeri powder was greater than that of wheat flour only.

The Quality Attributes of Chicken Meats Imported from Korea and Other Countries in Japanese Market (일본시장에 유통중인 한국산과 각국산 닭고기의 물리화학적 및 미생물학적 품질 비교)

  • 최일신;김일석;사메지마쿠니히코;민중석;이상옥;이무하
    • Food Science of Animal Resources
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    • v.21 no.4
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    • pp.307-313
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    • 2001
  • This study was carried out to examine the physicochemical and microbiological characteristics of Korean and other countries chicken circulated in Tokyo, Japan. The breast meats of Korean, Japanese, and Chinese chickens and thigh meats of Korean, Japanese, Chinese, American, and Thai chicken were used. In the proximate analysis of breast meat, Korean chicken had the lowest crude protein and crude ash(P<0.01), but showed the highest content of moisture(P<0.001). TBA values of Japanese breast meat were higher than Korean and Chinese one. In thigh meat, crude fat of American one was significantly higher than the others(P<0.001), and had very high bacterial counts. Korean chicken thigh meats showed the lowest VBN values.

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Changes of anthocyanidin, growth characteristics and brown rice yield of red colored rices at two region of eastern coast in Gyeongbuk province

  • Kim, Sang Kuk;Shin, Jong Hee;Kim, Se Jong
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.330-330
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    • 2017
  • The tallest culm length was observed in Geonganghongmi cultivar grown in coast area, and culm length was always taller in coast area than that of inland area among these three red rice cultivars. However, number of tiller per rice plant was not affected by different cultural area. Number of spikelets per panicle was much more increased in inland area. Regional difference in number of spikelets per panicle was only observed in Jeongjinju cultivar. Among these red rice cultivars, the highest brown rice yield was Jeongjinju rice cultivar having 702kg in inland area and 692kg in coast area, respectively. The $L^*$ values, which expresses the brightness, were in the range of 36.3 ~ 36.7, 36.7 ~ 37.0, and 38.7 ~ 39.6 in Jeogjinju, Hongjinju, Geonganghongmi rice cultivars, respectively. The $a^*$ values, which expresses the redness positively and greenness negatively were in the range of 12.3 ~ 12.8, 12.2 ~ 12.3, and 12.1 ~ 11.9 in Jeogjinju, Hongjinju, Geonganghongmi rice cultivars, respectively. In addition, the b values, which expresses the yellowness positively and blueness negatively were in the range of 13.8 ~ 14.0, 13.6 ~ 13.5, and 14.9 ~ 14.7 in Jeogjinju, Hongjinju, Geonganghongmi rice cultivars, respectively. Anthocyanidin content ranged 524 to $610{\mu}g/g$ dry weight basis. Cyanidin content was 11.4 to 14.0 times higher than that of delphinidin under coast and inland area. Among these rice cultivars, anthocyanidin content was always higher in rice cultivar grown at coast area. Highest head brown rice rate was only observed in Geonganghongmi to 95.2 at coast area and 95.4 inland area, respectively. Protein content was always higher in coast area than that of inland area. The lowest protein content was measured in Jeogjinju and amylose content was relatively increased in inland area compared to that of coast area. Consequently, in considered with brown rice yield and pigment content, Hongjinju rice cultivar was recommended in optimal pigment rice cultivar in eastern coast of Gyeongsangbuk-do Province.

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Effect of Adding Lactobacillus-Fermented Solution on Characteristics of Chicken Breast Meat (식물성 유산균 발효액 첨가에 따른 닭 가슴육의 특성에 미치는 효과)

  • Kim, Sun Hyo;Jayasena, Dinesh D.;Kim, Hyun-Joo;Jo, Cheorun;Jung, Samooel
    • Korean Journal of Poultry Science
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    • v.41 no.2
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    • pp.127-133
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    • 2014
  • The effect of Lactobacillus-fermented solution (LFS) at a concentration of 0, 1 and 2% on shelf-life extension in terms of total aerobic bacteria and on color, lipid oxidation, and sensorial characteristics of injected chicken breast meat was tested during a 9-day storage period at $4^{\circ}C$. Throughout the whole storage days, addition of LFS showed the significant inhibition of total aerobic bacteria counts in chicken breast meat compared with that of control. However, the addition of LFS to chicken breast meat resulted in the decrease of pH, the increase of $L^*$ and $b^*$ values, and the increase of lipid oxidation in chicken breast meat when compared with those of control at any given storage period (P<0.05). In addition, the chicken breast meat added with LFS was subjected to low scores in sensorial properties such as flavor, taste, tenderness, and overall acceptability. The results suggested that LFS can be used for improving the shelf-life of chicken meat processing product, however, further study to prevent the deterioration of quality such as lipid oxidation and sensorial property is needed.

Validation of Prediction Equations to Estimate the Energy Values of Feedstuffs for Broilers: Performance and Carcass Yield

  • Alvarenga, R.R.;Rodrigues, P.B.;Zangeronimo, M.G.;Makiyama, L.;Oliveira, E.C.;Freitas, R.T.F.;Lima, R.R.;Bernardino, V.M.P.
    • Asian-Australasian Journal of Animal Sciences
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    • v.26 no.10
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    • pp.1474-1483
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    • 2013
  • The objective was to evaluate the use of prediction equations based on the chemical composition of feedstuffs to estimate the values of apparent metabolisable energy corrected for nitrogen balance (AMEn) of corn and soybean meal for broilers. For performance and carcass characteristics, 1,200 one-d-old birds (male and female) were allotted to a completely randomised factorial $2{\times}8$ (two genders and eight experimental diets) with three replicates of each sex with 25 birds. In the metabolism trial, 240 eight-d-old birds were distributed in the same design, but with a split plot in time (age of evaluation) with five, four and three birds per plot, respectively, in stages 8 to 21, 22 to 35, and 36 to 42 d of age. The treatments consisted of the use of six equations systems to predict the AMEn content of feedstuffs, tables of food composition and AMEn values obtained by in vivo assay, totalling eight treatments. Means were compared by Scott-Knott test at 5% probability and a confidence interval of 95% was used to check the fit of the energy values of the diets to the requirements of the birds. As a result of this study, the use of prediction equations resulted in better adjustment to the broiler requirements, resulting in better performance and carcass characteristics compared to the use of tables, however, the use of energy values of feedstuffs obtained by in vivo assay is still the most effective. The best equations were: AMEn = 4,021.8-227.55 Ash (for corn) combined with AMEn = -822.33+69.54 CP-45.26 ADF+90.81 EE (for soybean meal); AMEn = 36.21 CP+85.44 EE+37.26 NFE (nitrogen-free extract) (for corn) combined with AMEn = 37.5 CP+46.39 EE+14.9 NFE (for soybean); and AMEn = 4,164.187+51.006 EE-197.663 Ash-35.689 CF-20.593 NDF (for corn and soybean meal).

Effects of stale maize on growth performance, immunity, intestinal morphology and antioxidant capacity in broilers

  • Liu, J.B.;Yan, H.L.;Zhang, Y.;Hu, Y.D.;Zhang, H.F.
    • Asian-Australasian Journal of Animal Sciences
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    • v.33 no.4
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    • pp.605-614
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    • 2020
  • Objective: This study was conducted to determine the effects of stale maize on growth performance, immunity, intestinal morphology, and antioxidant capacity in broilers. Methods: A total of 800 one-day-old male Arbor Acres broilers (45.4±0.5 g) were blocked based on body weight, and then allocated randomly to 2 treatments with 20 cages per treatment and 20 broilers per cage in this 6-week experiment. Dietary treatments included a basal diet and diets with 100% of control maize replaced by stale maize. Results: The content of fat acidity value was higher (p<0.05) while the starch, activities of catalase and peroxidase were lower (p<0.05) than the control maize. Feeding stale maize diets reduced (p<0.05) average daily feed intake (ADFI) throughout the experiment, feed conversion ratio (FCR) during d 0 to 21 and the whole experiment as well as relative weight of liver, spleen, bursa of Fabricius and thymus (p<0.05) on d 21. Feeding stale maize diets decreased jejunum villus height (VH) and VH/crypt depth (CD) (p<0.05) on d 21 and 42 as well as ileum VH/CD on d 42. The levels of immunoglobulin G, acid α-naphthylacetate esterase positive ratios and lymphocyte proliferation on d 21 and 42 as well as lysozyme activity and avian influenza antibody H5N1 titer on d 21 decreased (p<0.05) by the stale maize. Feeding stale maize diets reduced (p<0.05) serum interferon-γ, tumor necrosis factor-α, interleukin-2 on d 21 and interleukin-6 on d 21 and 42. Broilers fed stale maize diets had lower levels of (p<0.05) total antioxidative capacity on d 42, superoxide dismutase and glutathione peroxidase on d 21 and 42, but higher (p<0.05) levels of malondialdehyde on d 21 and 42. Conclusion: Feeding 100% stale maize decreased ADFI and FCR, caused adverse effects on immunity and antioxidant function and altered intestinal morphology in broilers.

Effects of Additives on the Quality Improvement of Minced Ginger (Zingiber officinale Roscoe) During Storage (생강(Zingiber officinale Roscoe) 다대기의 저장 중 품질개선을 위한 첨가물 효과)

  • Lee, Myung-Hee;Lee, Kyoung-Hae;Cho, Chang-Won;Kim, Kyung-Tack
    • Journal of Food Hygiene and Safety
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    • v.25 no.1
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    • pp.59-64
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    • 2010
  • The effects of vinegar, alcohol and ascorbic acid on the color, microorganism, sensory properties and flavor pattern of minced ginger (MG) were investigated during storage for 28 days at $30^{\circ}C$. The values of L (lightness), a (redness) and b (yellowness) of the control (T-0) and all the treatments changed slightly at the initial stage of storage, however the elapse of time accelerated the changes. The total bacterial counts of T-0 showed $5.37{\times}10^7\;CFU/g$ at the initial stage, but the MG-treatments decreased the bacteria above 4 log compared to T-0. It was showed that the additives were effective for inhibition of the growth of microorganism. Sensory properties of flavor intensity test showed no significant difference between T-0 and MG-treatments (p < 0.05). The result of volatile flavor contents of electronic nose analyzer (ENZ) showed that MG-treatments (T-I, T-II, T-III) was recognized stronger than non-treatment at the initial stage, but the change of flavor compound were stabilized soon regardless of type or quantity of additives during total storage period at $30^{\circ}C$.