Browse > Article

Efficacy of Mixture of Lactic Acid Bacteria (LAB) and Bifidobacteria Supplement in the Management of Constipation; Demonstration of Functionality in Animal and Clinical Trials  

Kim, Jung-Rae (Department of Pharmacy, Sahmyook University)
Lee, Do-Kyung (Department of Pharmacy, Sahmyook University)
Baek, Eun-Hye (Department of Pharmacy, Sahmyook University)
An, Hyang-Mi (Department of Pharmacy, Sahmyook University)
Yang, Hwan-Jin (Department of Pharmacy, Sahmyook University)
Kim, Mi-Jin (Department of Pharmacy, Sahmyook University)
Choi, Kyung-Soon (Department of Food and Nutrition, Sahmyook University)
Yun, Mi-Eun (Department of Food and Nutrition, Sahmyook University)
Jung, Yi-Jung (Department of Physical Therapy, Sahmyook University)
Oh, Pok-Ja (Department of Nursing, Sahmyook University)
Chung, Myung-Jun (CellBiotech, Co. Ltd)
Ha, Nam-Joo (Department of Pharmacy, Sahmyook University)
Publication Information
Korean Journal of Microbiology / v.46, no.1, 2010 , pp. 52-62 More about this Journal
Abstract
The aim of this study was to evaluated the efficacy of mixture of Lactic Acid Bacteria (LAB) and bifidobacteria supplement, which are contained with Lactobacillus acidophilus, Bifidobacterium longum SPM1205, and Pediococcus pentosaceus for the management of constipation in animal model and clinical trials. 5 ICR mice and 4 female constipation subjects were orally taken mixture of LAB and bifidobacteria for 2 weeks. We investigated the number of fecal LAB and harmful enzymes activities before and after mixture of LAB and bifidobacteria application. As a result, fecal LAB count was increased and harmful enzymes activities of intestinal microflora were generally decreased after mixture of LAB and bifidobacteria application. Also, 61 female subjects were randomly assigned to receive either mixture of LAB and bifidobacteria or lactose and were taken three times a day for 2 weeks. Then, we analyzed mixture of LAB and bifidobacteria effect through the questionnaires. Daily consumption of this mixture of LAB and bifidobacteria improved the constipation in constipation group (56.3%) compared with lactose application group (26.7%). Furthermore, after mixture of LAB and bifidobacteria treatment, frequency of hard stool decreased from 0.22 to 0.03. These results indicated that mixture of LAB and bifidobacteria application is effective to improve the constipation.
Keywords
animal models; clinical trials; management of constipation; mixture of lactic acid bacteria (LAB) and bifidobacteria supplement;
Citations & Related Records
Times Cited By KSCI : 3  (Citation Analysis)
Times Cited By SCOPUS : 1
연도 인용수 순위
1 Bub, S., J. Brinckmann, G. Cicconetti, and B. Valentine. 2006. Efficacy of an herbal dietary supplement (smooth move) in the management of constipation in nursing home residents: a randomized, double-blind, placebo-controlled study. J. Am. Med. Dir. Assoc. 7, 556-561.   DOI   ScienceOn
2 Diop, L., S. Guillou, and H. Durand. 2008. Probiotic food supplement reduces stress-induced gastrointestinal symptoms in volunteers: a double-blind, placebo-controlled, randomized trial. Nutr. Res. 28, 1-5.   DOI   ScienceOn
3 Kolars, J.C., M.D. Levitt, M.A. Aouji, and D.A. Savaiano. 1984. Yogurt-an autodigesting source of lactose. N. Engl. J. Med. 310, 1-3.   DOI   ScienceOn
4 Ly, S.Y., J.R. Shin, and S.H. Lim. 2003. Effect of drinking fermented milk on the improvement of defecation in constipated female students. Korean Living Science Assoc. 12, 265-273.   과학기술학회마을
5 Marteau, P., P. Pochart, B. Flourie, P. Pellier, L. Santos, J.F. Desjeux, and J.C. Rambaud. 1990. Effect of chronic ingestion of a fermented dairy product containing Lactobacillus acidophilus and Bifidobacterium bifidum on metabolic activities of the colonic flora in human. Am. J. Clin Nutr. 52, 685-688.   DOI
6 Martini, M.C., E.C. Lerebours, W.J. Lin, S.K. Harlander, N.M. Berrada, J.M. Antoine, and D.A. Savaiano. 1991. Strains and species of lactic acid bacteria in fermented milks (yogurt) : effect on in vivo lactose digestion. Am. J. Clin. Nutr. 54, 1041-1046.   DOI
7 McDonough, F.E., A.D. Hitchins, N.P. Wrong, P. Wells, and C.E. Bodwell. 1987. Modification of sweet acidophilus milk to improve utilization by lactose-intolerant persons. Am. J. Clin. Nutr. 45, 570-574.
8 Menino, M.J., C. Cutrin, R. Vieira, E. Barrio, and M.A. Parafita. 1993. Colchicine alters lactate utilization in isolated hepatocytes of rats treated with$ CCl_{4}$and ethanol. Life Sci. 52, 13-18.   DOI   ScienceOn
9 Muller-Lissner, S.A. 1988. Effect of wheat bran on weight of stool and gastrointestinal transit time: a meta analysis. Br Med. J. 296, 615-617.   DOI   ScienceOn
10 Park, S.Y., G.E. Ji, Y.T. Ko, H.K. Jung, Z. Ustunol, and J.J. Pestka. 1999. Potentiation of hydrogen peroxide, nitric oxide and cytokine production in RAW 264.7 macrophage cells exposed to human and commercial isolates of Bifidobacterium. Int. J. Food Microbiol. 46, 231-241.   DOI   ScienceOn
11 Grasten, S.M., K.S. Juntunen, J. Matto, O.T. Mykkqanen, H. El-Nezami, H. Adlercreutz, K.S. Poutanen, and H.M. Mykkanen. 2007. High-fiber rye bread improves bowel function in postmenopausal women but does not cause other putatively positive change in the metabolic activity of intestinal microbiota. Nutr. Res. 27, 454-461.   DOI   ScienceOn
12 Guarner, F. and G.J. Schaafsma. 1998. Probiotics. Int. J. Food Microbiol. 39, 237-238.   DOI   ScienceOn
13 Hans-Peter, B., S. Wolfgang, G. Stephanie, R. Gotthard, K. Elisabeth, and K. Heinrich. 1994. Does yogurt enriched with Bifidobacterium longum affect colonic microbiology and fecal merabolites in healthy subjects? Am. J. Clin. Nutr. 59, 428-432.   DOI
14 Harrison, V.C. and G. Peat. 1975. Serum cholesterol and bowel flora in the newborn. Am. J. Clin. Nutr. 28, 1351-1355.
15 Hicks, A. 2001. The prevention and management of constipation. J. Orthop. Nurs. 5, 208-211.   DOI   ScienceOn
16 Hilton, E., H.D. Isenberg, P. Alperstein, K. France, and M.T. Borenstein. 1992. Ingestion of yogurt containing Lactobacillus acidophilus as prophylaxis for candidal vaginitis. Ann. Intern. Med. 116, 353-357.   DOI
17 Kim, J.H., H.J. Kim, J.H. Son, H.N. Chun, J.O. Yang, S.J. Choi, N.S. Park, G.H. Choi, and S.K. Kim. 2003. Effect of Lactobacillus fermentum MG590 on alchole metabolism and liver function in rats. J. Microbiol. Biotechnol. 13, 919-925.   과학기술학회마을
18 Kim, D.H., H.J. Kang, S.W. Kim, and K. Kobayashi. 1992. pH-inducible $\beta$-glucuronidase and$\beta$-glucosidase of intestinal bacteria. Chem. Pharm. Bull. 40, 1967-1969.
19 Kim, D.H., J.H. Lee, E.A. Bae, and M.J. Han. 1995. Induction and inhibition of indole of intestinal bacteria. Arch. Pharm. Res. 18, 351-533.   DOI
20 Kim, H.S. and S.E. Gilliland. 1983. Lactobacillus acidophilus as a dietary adjunct for milk to aid lactose digestion in humans. J. Dairy Sci. 66, 959-966.   DOI   ScienceOn
21 Coronado, B.E., S.M. Opal, and D.C. Yoburn. 1995. Antibioticinduced D-lactic acidosis. Ann. Intern. Med. 122, 839-842.   DOI
22 D'Aimmo, M.R., M. Modesto, and B. Biavati. 2007. Antibiotic resistance of lactic acid bacteria and Bifidobacterium spp. isolated from dairy and pharmaceutical products. Int. J. Food Microbiol. 115, 35-42.   DOI   ScienceOn
23 Faigel, D.O. 2002. A clinical approach to constipation. Clin. Cornerstone 4, 11-18.   DOI   ScienceOn
24 Fernandez-Banares, F. 2006. Nutritional care of the patient with constipation. Best Pract. Res. Clin. Gastroenterol. 20, 575-587.   DOI
25 Fuller, R. 1989. A review: probiotics in man and animals. J. Appl. Bacteriol. 66, 365-378.   DOI
26 Gill, H.S. and F. Guarner. 2004. Probiotics and human health: a clinical perspective. Postgrad. Med. J. 80, 516-526.   DOI   ScienceOn
27 Goldin, B.R., L. Swenson, J. Dwyer, M. Sexton, and S.L. Gorbach. 1980. Effect of diet and Lactobacillus acidophilus supplements on human fecal bacterial enzymes. J. Natl. Cancer Inst. 64, 255-261.   DOI
28 Gill, H.S., K.J. Rutherfurd, J. Prasad, and P.K. Gopal. 2000. Enhancement of natural and acquired immunity by Lactobacillus rhamnosus (HN001), Lactobacillus acidophilus (HN017) and Bifidobacterium lactis (HN019). Br. J. Nutr. 83, 167-176.   DOI   ScienceOn
29 Gilliland, S.E. and M.L. Speck. 1977. Antagonistic action of Lactobacillus acidophilus toward intestinal and foodborne pathogens in associative cultures. J. Food Prot. 40, 820-823.   DOI
30 Goldin, B.R. and S.L. Gorbach. 1984. Alteration of the intestinal microflora by diet, oral antibiotics, and Lactobacillus: decreased production of free amines from aromatic nitro compounds, azo dyes and glucuronides. J. Natl. Cancer Inst. 73, 689-695.
31 Ahn, T.S. 1999. Loosing the constipation by capsulated yogurt. J. Microbiol. 35, 94-97.
32 Ayebo, A.D., I.A. Angelo, and K.M. Shahani. 1980. Effect of ingesting Lactobacillus acidophilus milk upon fecal flora and enzyme activity in humans. Milchwissenschaft 35, 730-733.
33 Bessis, D., A. Le Quellec, A. Sotto, C. Perez, and A.J. Ciurana. 1995. Lactobacillus acidophilus endocarditis after an appendectomy. Clin. Infect. Dis. 20, 724-725.   DOI   ScienceOn
34 Bharucha, A.E. 2007. Constipation. Best Pract. Res. Clin. Gastroenterol. 21, 709-731.   DOI
35 Choi, S.S., B.Y. Kang, M.J. Chung, S.D. Kim, S.H. Park, J.S. Kim, C.Y. Kang, and N.J. Ha. 2005. Safety assessment of potential lactic acid bacteria Bifidobacterium longum SPM1205 isolated from healthy Koreans. J. Microbiol. 43, 493-498.   과학기술학회마을
36 Biavati, B., M. Vescovo, S. Torriani, and V. Bottazzi. 2000. Bifidobacteria: history, ecology, physiology and applications. Ann. Microbiol. 50, 117-131.
37 Bosaeus, I. 2004. Fibre effects on intestinal functions (diarrhoea, constipation and irritable bowel syndrome). Clin. Nutr. Suppliments 1, 33-38.
38 Chen, C., S. Louie, N.S. Hai, and W. Walker. 2005. Preinoculation with the probiotic Lactobacillus acidophilus early in life effectively inhibits murine Citrobacter rodentium colitis. Pediatr. Res. 58, 1185-1191.   DOI   ScienceOn
39 Corinne, G.D., C. Claire, P. Christophe, P. Francoise, V. Pierre, B. Christine, S. Odette, and A. Claude. 1998. Milk fermented with yogurt cultures and Lactobacillus casei compared with yogurt and gelled milk: influence on intestinal microflora in healthy infants. Am. J. Clin. Nutr. 67, 111-117.   DOI
40 Salminen, S., A. von Wright, L. Morelli, P. Marteau, D. Brassart, W.M. de Vos, R. Fondén, and et al. 1998. Demonstration of safety of probiotics-a review. Int. J. Food Microbiol. 44, 93-106.   DOI
41 Vikrant, K., A. Seema, M. Ashraf, and M. Abida. 2005. Efficacy of tyndalized Lactobacillus acidophilus in acute diarrhea. Indian J. Pediatr. 72, 935-938.   DOI   ScienceOn
42 Wood, S.I. 1995. Are health professional guilty of laxative abuse. Pharm. J. 255, 659-661.
43 Yun, J.H., D.S. Yim, J.Y. Kang, B.Y. Kang, E.A. Shin, M.J. Chung, S.D. Kim, D.H. Baek, S.S. Choi, and N.J. Ha. 2005. Identification of Lactobacillus ruminus SPM0211 isolated from healthy Korean and screening for the antimicrobial activity of this strain against some pathogens. Arch. Pham. Res. 28, 660-666.   DOI
44 Rao, C.V., M.E. Sanders, C. Indranie, B. Simi, and B.S. Reddy. 1999. Prevention of colonic preneoplastic lesions by the probiotic Lactobacillus acidophilus NCFM in F344 rats. Int. J. Oncol. 14, 939-944.
45 Pedrosa, M.C., B.B. Golner, B.R. Goldin, S. Barakat, G.E. Dallal, and R.M. Russell. 1995. Survival of yogurt-containing organisms and Lactobacillus gasseri (ADH) and their effect on bacterial enzyme activity in the gastrointestinal tract of healthy and hypochlorhydric elderly subjects. Am. J. Clin. Nutr. 61, 353-359.   DOI
46 Pochart, P., O. Dewit, J.F. Desjeux, and P. Bourlioux. 1989. Viable starter culture,$\beta$-galactosidase activity and lactose in duodenum after yogurt ingestion in lactase-deficient human. Am. J. Clin. Nutr. 49, 828-831.   DOI
47 Pochart, P., P. Marteau, Y. Bouhnik, I. Goderel, P. Bourlioux, and J.C. Rambaud. 1992. Survival of bifidobacteria ingested via fermented milk during their passage through the human small intestine: and in vivo study using intestinal perfusion. Am. J. Clin. Nutr. 55, 78-80.