• Journal of Chest Surgery
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• 제21권6호
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• pp.970-978
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• 1988

There is tendency of increasing number and decreasing age of patients who are indicated for Rastelli operation for their cyanotic congenital heart disease. So there is the need to find the criterion which saves the patients from early postoperative hemodynamic disturbances. We reviewed the 26 patients who had been performed Rastelli operation at Seoul national University Hospital from January 1981 to June 1988. mean age of the patients was 7.8*3.4 years[range 2.5-15years], mean body surface area[BSA] 0.79*0.25m2[range 0.49-1.51m2] and mean hematocrit 57.95*12%[range 48-80%]. We divided these patients into survived group and died group before postoperative 72 hours, and analyzed preoperative arterial oxygen saturation[SaO2], the ratio of diameter of right pulmonary artery to ascending aorta[RPA/AA], the ratio of both right and left pulmonary artery diameter to descending thoracic aorta[RPA+LPA/DTA], pulmonary artery index[PA index], cardiopulmonary bypass time, aorta cross-clamping time, postoperative perfusion state and total amount of dopamine infused postoperatively. The results showed that RPA+LPA/DTA and PA index were statistically significant factors to influence early postoperative cardiac death rate[P< 0.05]. Especially there were good linear correlations between PA index[X] and peripheral perfusion index[Y][Y= - 1.15+0.02 X, r=0.86, P<0.01]and between PA index[X] and total amount of dopamine infused before postoperative 72 hours[mg/kg, Y][Y=61.94 - 0.15 X, r=-0.80, P < 0.01]. Also there were tendencies that the higher RPA+LPA/DTA[Y], the better peripheral perfusion [X] and the lower need of dopamine[X], but no statistical significance.[Y=0.78+1.60 X, r =0. 49, P >0.05] And the discriminate analysis showed that patients with PA index over 221 mm2/BSA could undergo correction with 25 per cent of error rate. In conclusion, early postoperative hemodynamic states could be predicted by preoperatively measured PA index, and which can be used as a criterion for Rastelli operation performed on cyanotic congenital heart disease.

• 한국전자파학회:학술대회논문집
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• 한국전자파학회 2001년도 종합학술발표회 논문집 Vol.11 No.1
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• pp.243-246
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• 2001

A cavity delay filter module for IMT-2000 LPA has been developed with 0.9dB insertion loss, 0.1dB/0.6$^{\circ}$ gain/phase flatness. Broadband signal cancellation of the module has been simulated using the measured S parameter, and gain, phase and group delay have been matched to satisfy the signal cancellation of the module. In the experiment, the value of designed parameters are used and 40dB signal cancellation has been obtained over 100MHz bandwidth. Also the sampling cancellation has been shown to be the similar performance of the feedforward output cancellation.

• 한국전자파학회논문지
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• 제17권10호
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• pp.959-966
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• 2006

본 논문은 다이플렉서를 이용하여 디지털 셀룰라 대역($f_o$=880 MHz)과 IMT-2000 대역($f_o$=2,140 MHz) 기지국에서 동시에 사용 가능한 이중 대역 전치 왜곡 선형 전력 증폭기(Predistortion Linear Power Amplifier: PD LPA)의 설계 방법을 제시하였다. 입 출력단에 사용된 다이플렉서는 결함 접지 구조(Defected Ground Structure: DGS)를 이용한 저역 통과 여파기와 높은 Q값을 갖는 캐패시터와 마이크로 스트립 스터브를 이용한 고역 통과 여파기로 이루어져 있다. 입출력 반사 계수 특성을 좋게 하기 위하여 3 dB 하이브리드 결합기를 이용한 반사형 타입의 전치 왜곡 선형화기를 설계하였다. IS-95 CDMA IFA 신호와 WCDMA IFA 신호를 이용하여 각 대역에서 제작된 이중 대역 전치 왜곡 선형 전력 증폭기의 인접 채널 누설비(Adjacent Channel Leakage Ratio: ACLR) 개선 정도를 측정한 결과 880 MHz 대역에서 약 10 dB, 2,140 MHz 대역에서 약 9.36 dB 개선되었다.

• 디지털융복합연구
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• 제16권1호
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• pp.439-452
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• 2018

본 연구는 경제활동에 참여하는 은퇴 전 한국 베이비부머의 소비유형을 실증적으로 탐색하고, 유형별 소비가 우울에 미치는 영향을 검증하는 데 목적을 두고 있다. 잠재프로파일분석을 이용하여 베이비부머의 소비를 분석한 결과, 기초생활중심형, 사회생활중심형, 주거생활중심형, 고급소비중심형의 4가지 유형이 구조화되었다. 이러한 소비유형과 우울은 유의미한 상관관계가 있는 것으로 증명되었는데, 특히 사회생활중심형 소비유형과 비교하여 기초생활중심형과 고급소비중심형의 우울수준이 유의하게 높았다. 또한 교육수준이 낮을수록, 배우자가 없는 경우, 주관적 건강상태가 나쁜 경우, 신체기능에 제한이 있을수록, 장애진단을 받을수록, 여가활동에 만족하지 않을수록, 소득이 낮은 경우, 주관적 경제상태가 부정적일 때 우울수준이 높은 것으로 조사되었다. 본 연구결과를 바탕으로 한국 베이비부머를 위한 특화된 노후준비 정책과 프로그램의 개발 필요성을 제언한다.

• Journal of Ginseng Research
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• 제45권5호
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• pp.575-582
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• 2021

Background: In ginseng, there exists a glycolipoprotein complex with a special form of lipid LPAs called Gintonin. The purpose of this study is to show that Gintonin has a therapeutic effect on rheumatoid arthritis through LPA2 receptors. Methods: Fibroblast-like synoviocytes (FLS) were treated with Gintonin and stimulated with interleukin (IL)-1β. The antioxidant effect of Gintonin was measured using MitoSOX and H₂DCFDA experiments. The anti-arthritic efficacy of Gintonin was examined by analyzing the expression levels of inflammatory mediators, phosphorylation of mitogen-activated protein kinase (MAPK) pathways, and translocation of nuclear factor kappa B (NF-κB)/p65 into the nucleus through western blot. Next, after treatment with LPAR2 antagonist, western blot analysis was performed to measure inflammatory mediator expression levels, and NF-κB signaling pathway. Carrageenan/kaolin-induced arthritis rat model was used. Rats were orally administered with Gintonin (25, 50, and 100 mg/kg) every day for 6 days. The knee joint thickness, squeaking score, and weight distribution ratio (WDR) were measured as the behavioral parameters. After sacrifice, H&E staining was performed for histological analysis. Results: Gintonin significantly inhibited the expression of iNOS, TNF-α, IL-6 and COX-2. Gintonin prevented NF-κB/p65 from moving into the nucleus through the JNK and ERK MAPK phosphorylation in FLS cells. However, pretreatment with an LPA2 antagonist significantly reversed these effects of Gintonin. In the arthritis rat model, Gintonin suppressed all parameters that were measured. Conclusion: This study suggests that LPA2 receptor plays a key role in mediating the anti-arthritic effects of Gintonin by modulating inflammatory mediators, the MAPK and NF-κB signaling pathways.

• Journal of Ginseng Research
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• 제47권3호
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• pp.390-399
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• 2023

Background: Gintonin (GT), a Panax ginseng-derived lysophosphatidic acid receptor (LPAR) ligand, has positive effects in cultured or animal models for Parkinson's disease, Huntington's disease, and so on. However, the potential therapeutic value of GT in treating epilepsy has not yet been reported. Methods: Effects of GT on epileptic seizure (seizure) in kainic acid [KA, 55mg/kg, intraperitoneal (i.p.)]-induced model of mice, excitotoxic (hippocampal) cell death in KA [0.2 ㎍, intracerebroventricular (i.c.v.)]-induced model of mice, and levels of proinflammatory mediators in lipopolysaccharide (LPS)-induced BV2 cells were investigated. Results: An i.p. injection of KA into mice produced typical seizure. However, it was significantly alleviated by oral administration of GT in a dose-dependent manner. An i.c.v. injection of KA produced typical hippocampal cell death, whereas it was significantly ameliorated by administration of GT, which was related to reduced levels of neuroglial (microglia and astrocyte) activation and proinflammatory cytokines/enzymes expression as well as increased level of the Nrf2-antioxidant response via the upregulation of LPAR 1/3 in the hippocampus. However, these positive effects of GT were neutralized by an i.p. injection of Ki16425, an antagonist of LPA1-3. GT also reduced protein expression level of inducible nitric-oxide synthase, a representative proinflammatory enzyme, in LPS-induced BV2 cells. Treatment with conditioned medium clearly reduced cultured HT-22 cell death. Conclusion: Taken together, these results suggest that GT may suppress KA-induced seizures and excitotoxic events in the hippocampus through its anti-inflammatory and antioxidant activities by activating LPA signaling. Thus, GT has a therapeutic potential to treat epilepsy.

• Journal of Ginseng Research
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• 제48권1호
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• pp.1-11
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• 2024

Fresh ginseng is prone to spoilage due to its high moisture content. For long-term storage, most fresh ginsengs are dried to white ginseng (WG) or steamed for hours at high temperature/pressure and dried to form Korean Red ginseng (KRG). They are further processed for ginseng products when subjected to hot water extraction/concentration under pressure. These WG or KRG preparation processes affect ginsenoside compositions and also other ginseng components, probably during treatments like steaming and drying, to form diverse bioactive phospholipids. It is known that ginseng contains high amounts of gintonin lysophosphatidic acids (LPAs). LPAs are simple lipid-derived growth factors in animals and humans and act as exogenous ligands of six GTP-binding-protein coupled LPA receptor subtypes. LPAs play diverse roles ranging from brain development to hair growth in animals and humans. LPA-mediated signaling pathways involve various GTP-binding proteins to regulate downstream pathways like [Ca²⁺]_i transient induction. Recent studies have shown that gintonin exhibits anti-Alzheimer's disease and antiarthritis effects in vitro and in vivo mediated by gintonin LPAs, the active ingredients of gintonin, a ginseng-derived neurotrophin. However, little is known about how gintonin LPAs are formed in high amounts in ginseng compared to other herbs. This review introduces atypical or non-enzymatic pathways under the conversion of ginseng phospholipids into gintonin LPAs during steaming and extraction/concentration processes, which exert beneficial effects against degenerative diseases, including Alzheimer's disease and arthritis in animals and humans via LPA receptors.

• 한국융합학회논문지
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• 제13권4호
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• pp.625-634
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• 2022

이 연구의 목적은 지역에 거주하는 65세 이상 노인의 신체 활동(physical activity; PA) 수준과 활동 체력(functional fitness; FF) 변인 사이의 관계를 조사하는 것이었다. 피검자(남성:1,645명; 여성:2,394명)의 PA 수준은 설문지를 이용하여 조사하였고, FF는 상·하지 근력, 민첩성 및 동적 평형성, 유연성, 유산소성 능력, 협응력, 그리고 신체 구성 등 체력 영역을 대표하는 6항목으로 측정, 평가하였다. 피검자는 신체 활동 권장기준에 따라 권장량보다 낮은 그룹(low physical activity; LPA)과 권장량 기준을 충족하는 그룹(PA)으로 나누었고, PA 그룹은 다시 강도 수준에 따라 중강도 그룹(MIPA)과 고강도 그룹(HIPA)으로 분류하였다. 권장량 수준을 충족하는 피검자는 48%였고, 이들은 모든 항목에서 LPA보다 성적이 높았다(p<.001). HIPA 그룹은 MIPA 그룹보다 민첩성 및 동적 평형성, 협응력, 그리고 하지 근력(여성)이 유의하게 높았다(p<.001). 결론적으로 지역에 거주하고 있는 노인이 활동 체력을 유지하고 개선하기 위해서는 건강을 위해 권장하고 있는 신체 활동량을 충족시켜야 하며, 특히 낙상 관련 체력을 개선하기 위해서는 강도가 높은 신체 활동을 할 필요가 있다.

• Biomolecules & Therapeutics
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• 제14권1호
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• pp.25-29
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• 2006

We tested effects of bioactive lysophospholipids including lysophosphatidic acid (LPA), lysophosphatidylcholine (LPC), sphingosylphosphorylcholine (SPC), and sphingosine I-phosphate (S1P) on membrane potential in C6 glioma cells to understand action mechanism of the lysophospholipids. Membrane potential was estimated by measuring fluorescence change of DiBAC-loaded glioma cells. LPA largely increased membrane potential and the increase was gradually diminished. LPC also increased the membrane potential, however, the increase sustained. SPC induced smaller increase of membrane potential than LPC. SIP was not able to change the membrane potential. We tested effects of suramin and pertussis toxin on lysophospholipid-induced membrane potential increase. However, there wasn't any effect. The membrane potential increase was partially diminished in $Na^+$-free media, suggesting $Na^+$ influx as a component of membrane potential changes. Thus, involvement of $Na^+$ influx in the increase of membrane potential by lysophospholipids and independence of suramin-sensitive GPCRs and pertussis toxin-sensitive G proteins are found in this study.

• Molecules and Cells
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• 제37권9호
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• pp.656-663
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• 2014

Gintonin, a novel, ginseng-derived G protein-coupled lysophosphatidic acid (LPA) receptor ligand, elicits $[Ca^{2+}]_i$ transients in neuronal and non-neuronal cells via pertussis toxin-sensitive and pertussis toxin-insensitive G proteins. The slowly activating delayed rectifier $K^+$ ($I_{Ks}$) channel is a cardiac $K^+$ channel composed of KCNQ1 and KCNE1 subunits. The C terminus of the KCNQ1 channel protein has two calmodulin-binding sites that are involved in regulating $I_{Ks}$ channels. In this study, we investigated the molecular mechanisms of gintonin-mediated activation of human $I_{Ks}$ channel activity by expressing human $I_{Ks}$ channels in Xenopus oocytes. We found that gintonin enhances $I_{Ks}$ channel currents in concentration- and voltage-dependent manners. The $EC_{50}$ for the $I_{Ks}$ channel was $0.05{\pm}0.01{\mu}g/ml$. Gintonin-mediated activation 1 of the $I_{Ks}$ channels was blocked by an LPA1/3 receptor antagonist, an active phospholipase C inhibitor, an $IP_3$ receptor antagonist, and the calcium chelator BAPTA. Gintonin-mediated activation of both the $I_{Ks}$ channel was also blocked by the calmodulin (CaM) blocker calmidazolium. Mutations in the KCNQ1 $[Ca^{2+}]_i$/CaM-binding IQ motif sites (S373P, W392R, or R539W)blocked the action of gintonin on $I_{Ks}$ channel. However, gintonin had no effect on hERG $K^+$ channel activity. These results show that gintonin-mediated enhancement of $I_{Ks}$ channel currents is achieved through binding of the $[Ca^{2+}]_i$/CaM complex to the C terminus of KCNQ1 subunit.