• Journal of Korean Society on Water Environment
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• v.31 no.3
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• pp.241-252
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• 2015

Runoff behaviors by five bias correction methods were analyzed, which were Change Factor methods using past observed and estimated data by the estimation scenario with average annual calibration factor (CF_Y) or with average monthly calibration factor (CF_M), Quantile Mapping methods using past observed and estimated data considering cumulative distribution function for entire estimated data period (QM_E) or for dry and rainy season (QM_P), and Integrated method of CF_M+QM_E(CQ). The peak flow by CF_M and QM_P were twice as large as the measured peak flow, it was concluded that QM_P method has large uncertainty in monthly runoff estimation since the maximum precipitation by QM_P provided much difference to the other methods. The CQ method provided the precipitation amount, distribution, and frequency of the smallest differences to the observed data, compared to the other four methods. And the CQ method provided the rainfall-runoff behavior corresponding to the carbon dioxide emission scenario of SRES A1B. Climate change scenario with bias correction still contained uncertainty in accurate climate data generation. Therefore it is required to consider the trend of observed precipitation and the characteristics of bias correction methods so that the generated precipitation can be used properly in water resource management plan establishment.

• Journal of Life Science
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• v.33 no.11
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• pp.851-858
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• 2023

Unique cartilage matrix-associated protein (UCMA) is an extrahepatic vitamin K-dependent protein rich in γ-carboxylated (Gla) residues. UCMA has been recognized for its ability to promote osteoblast differentiation and enhance bone formation; however, its impact on osteoblasts under hyperglycemic stress remains unknown. In this paper, we investigated the effect of UCMA on MC3T3-E1 osteoblastic cells under hyperglycemic conditions. After exposure to high glucose, the MC3T3-E1 cells were treated with recombinant UCMA proteins. CellROX and MitoSOX staining showed that the production of reactive oxygen species (ROS), which initially increased under high-glucose conditions in MC3T3-E1 cells, decreased after UCMA treatment. Additionally, quantitative polymerase chain reaction revealed increased expression of antioxidant genes, nuclear factor erythroid 2-related factor 2 and superoxide dismutase 1, in the MC3T3-E1 cells exposed to both high glucose and UCMA. UCMA treatment downregulated the expression of heme oxygenase-1, which reduced its translocation from the cytosol to the nucleus. Moreover, the expression of dynamin-related protein 1, a mitochondrial fission marker, was upregulated, and AKT signaling was inhibited after UCMA treatment. Overall, UCMA appears to mitigate ROS production, increase antioxidant gene expression, impact mitochondrial dynamics, and modulate AKT signaling in osteoblasts exposed to high-glucose conditions. This study advances our understanding of the cellular mechanism of UCMA and suggests its potential use as a novel therapeutic agent for bone complications related to metabolic disorders.

• Journal of Physiology & Pathology in Korean Medicine
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• v.28 no.1
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• pp.63-68
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• 2014

The fruit of Tribulus terrestris L. (Zygophyllaceae) is an important source of traditional Korean and Chinese medicines. In this study, NNMBS223, consisting of the ethanol extract of T. terrestris, showed potent anti-inflammatory activities in RAW264.7 macrophages. We investigated the effect of NNMBS223 in suppressing the protein expression of inducible nitric oxide synthase (iNOS), cyclooxygenase (COX)-2 and production of iNOS-derived nitric oxide (NO), COX-2-derived prostaglandin E2 (PGE2) in lipopolysaccharide (LPS)-stimulated macrophages. In addition, NNMBS223 induced expression of heme oxygenase (HO)-1 through nuclear translocation of nuclear factor E2-related factor 2 (Nrf2) in macrophages. The effects of NNMBS223 on LPS-induced production of NO and PGE2 were partially reversed by the HO activity inhibitor tin protoporphyrin (SnPP). These findings suggest that Nrf2-dependent increases in expression of HO-1 induced by NNMBS223 conferred anti-inflammatory activities in LPS stimulated RAW264.7 macrophages.

• Proceedings of the KIPE Conference
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• 2017.07a
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• pp.88-89
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• 2017

This paper investigates the design of multi-winding coupled inductor for minimum inductor current ripple in rapid traction battery charger systems. Based on the general circuit model of multi-winding coupled inductor together with the operating principles of dc-dc converter, the relationship between the ripple size of inductor current and the coupling factor is derived under the different duty ratio. The optimal coupling factor which corresponds to a minimum inductor ripple current becomes -(1/n-1), i.e. a complete inverse coupling without leakage inductance, as the steady-state duty ratio operating point approaches 1/n, 2/n, … or (n1)/n. In an opposite manner, the optimal coupling factor value of zero, i.e. zero mutual inductance, is required when the steady-state duty ratio operating point approaches either zero or one.

• Korean Journal of Microbiology
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• v.32 no.4
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• pp.264-270
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• 1994

A gene coding for a novel putative $\sigma$ factor of RNA polymerase has been identified from Streptomyces coelicolor A3(2) using Escherichia coli rpoS gene fragment as a probe. The 486 bp rpoS gene fragment was amplified from E. coli genomic DNA by PCR with two synthetic oligonucleotides, the sequences of which were deduced from the amino acid sequences in the regions 2.3 and 4.2 conserved among various bacterial factors. When E. coli genomic DNA fragments were hybridized with cloned rpoS probe, only one band corresponding to rpoS gene (3.2 kb PvuII fragment or 2.3 kb KpnI fragment) was detected. In S. coelicolor, however, two bands were detected both in PvuII digested DNA and SalI digested DNA. 3.5 kb PvuII fragment which binds the rpoS gene probe was cloned (pMS1) from the sublibrary, and the nucleotide sequences of 1.0 kb BamH'/HincII subclone (pBH2) was partially determined. The nucleotide sequences revealed extensive similarity to other $\sigma$ factor genes of S. coelicolor (hrdA, hrdB, hrdC, hrdD), S. aureofaciens (hrdA, hrdB, hrdC, hrdD), Synechococcus species, Pseudomonas aeruginosa, Stigmatella aurantiaca, and Anabaena species. The nucleotide sequences in regions 1.2 and 4 were compared with the corresponding regions of 5 known ${\sigma}$ factor genes of S. coelicolor by multiple alignment. It turned out that the cloned gene is most closely related to hrdA showing 88% amino acid similarity in region 1.2 and 75% in region 4.

• Proceedings of the KIEE Conference
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• 1996.07a
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• pp.385-387
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• 1996

A novel two stage soft-switching ac-to-dc convener with power factor correction is proposed. The proposed convener provides zero-voltage-switching (ZVS) condition to main switch of boost pre-regulator without auxiliary switch. Comparing to the conventional two stage approach(ZVS-PWM boost rectifier followed by off-line ZVS dc-dc step down converter), the proposed approach is simple and reducing EMI noise problem. A new simple DC-linked energy feedback circuit provides zero-voltage-switching condition to boost pre-regulator without imposing additional voltage and current stresses and loss of PWM capability. Operational principle, analysis, control of the proposed converter together with the simulation results of 1KW prototype are presented.

• Microbiology and Biotechnology Letters
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• v.33 no.3
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• pp.189-193
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• 2005

IgE-dependent histamine-releasing factor(HRF) was initially described as a secretagogue for secretion of histamine from IgE+ basophils from a subset of allergic donors. Previously, we identified that S98 residue of HRF was phosphorylated using anti-HRFpS98 antibody which specifically recognizes the phosphorylated serine residue of HRF and HRFS98A mutant construct. In vitro kinase assay, only wild type HRF was phosphorylated by PKC, and S98A HRF was not affected by PKC. In this study, we attempted to characterize the phosphatase which specifically dephosphorylates HRF by immunoprecipitation and pull-down assay. In RBL-2H3 cells, HRF interacted only with calcineurin (also called as PP2B, calcium/calmodulin-dependent phosphatase) but not with PP1 or PP2A. The results suggest that HRF is most likely dephosphory-lated by calcineurin.

• The Journal of the Korea institute of electronic communication sciences
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• v.18 no.6
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• pp.1031-1040
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• 2023

Propagation characteristics in line-of-sight(LOS) paths in 3, 6, 10, and 17 GHz frequency bands were measured and analyzed in two different indoor corridors: second floors of Buildings D2 and E2. The measurement was designed to measure when the receiving antenna moved at 0.5 m intervals from 3 m to 30 m, while the transmission antenna was fixed. The analysis of the two indoor corridors was compared by applying basic transmission loss, root mean square (RMS) delay spread, and K-factor. For basic transmission loss, the loss coefficient of the floating intercept path loss model was higher in the indoor corridor of Building E2 than in that of Building D2. Similarly, the RMS delay spread in the time domain was greater in the indoor corridor of Building E2. However, the indoor corridor of Building D2 exhibited higher K-factor in the 3, 6, and 17 GHz bands with lower wave propagation in the 10 GHz band. Despite the 2 indoor corridors being identical, the propagation characteristics varied due to different internal structures and materials. The results provide measurement data for ITU-R Recommendations regarding various indoor environments.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.30 no.4 s.48
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• pp.479-483
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• 2004

An attempt was made to develop a proteinchip for screening of MITF (microphthalmia transcription factor) inhibitor. Binding of MITF to E-box causes transcription of several pigmenting genes including tyrosinase gene. We investigated binding of MITF and its DNA binding site (E-box) using a protein-DNA chip with various detection methods including flurorescence (Cyt3). SPR (surface plasmon resonance) and SPRi (surface plasmon resonance imaging). A fusion protein (MITF-Maltose Binding Protein) was attached on the glass plate by chemical modification. An inhibitory synthetic DNA oligomer, artificially designed based on the E-box sequence, inhibited the binding of MITF and E-box. These results showed the potentials of flurorescence-based MITF protein chip as a microarray for high throughput screening (HTS) system of depigmenting agents.

• Journal of Fashion Business
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• v.7 no.1
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• pp.38-54
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• 2003

This study looked into the present status of e-mail marketing, which is conducted in local Internet fashion shopping malls and is being used as a one-to-one marketing method in the e-CRM concept so as to establish an effective e-mail marketing policy by grasping problems based on the current status and by presenting solutions to the problems. This study analyzed the e-mail letters received during a certain period of time by 30 professional Internet fashion shopping malls of with famous goods, brands and non-brands. Four individuals subscribed to shopping mall through the procedure for membership applying for e-mail services. The analysis showed that there were no shopping malls that were doing a differentiated e-mail marketing among Internet shopping malls. As for time factor, they were sending e-mail letters irregularly regardless of a certain cycle. And as for visual factor, they didnt have any standard for the content of the mail. As for content factor, the contents of their mail were not differentiated and all the same. The comparison of fashion malls showed that the brand shopping malls were the most active in e-mail marketing. On the basis of the problems, the direction for the effective e-mail marketing of Internet shopping malls are presented as follows: First, individualized contents fitting for customers should be developed, and differentiated mailing services should be provided thereby. Second, each shopping mall should make periodic tests of mailing to find out the optimal time and day to provide e-mail services to the customers at the designated time. Third, e-mail should be differentiated fitting for the website according to a certain on a certain standard for visual effects and the identity of shopping mall should be established. Firth, in the contents of e-mail, the image and message elements should be properly harmonized and creative policies should be made with interactive or recreational elements. Fifth, a homepage policy which can satisfy customers demand for information when the e-mail is clicked on..