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Manufacturing Protein-DNA Chip for Depigmenting Agent Screening  

Han Jung-Sun (Department of Biological Engineering, Inha University)
Kwak Eun-Young (Department of Biological Engineering, Inha University)
Lee Hyang-Bok (Department of Biological Engineering, Inha University)
Shin Jlung-Hyun (Department of Dermatology, College of Medicine, Inha University)
Baek Seung-Hak (Bionanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology)
Chung Bong-Hyun (Bionanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology)
Kim Eun-Ki (Department of Biological Engineering, Inha University)
Publication Information
Journal of the Society of Cosmetic Scientists of Korea / v.30, no.4, 2004 , pp. 479-483 More about this Journal
Abstract
An attempt was made to develop a proteinchip for screening of MITF (microphthalmia transcription factor) inhibitor. Binding of MITF to E-box causes transcription of several pigmenting genes including tyrosinase gene. We investigated binding of MITF and its DNA binding site (E-box) using a protein-DNA chip with various detection methods including flurorescence (Cyt3). SPR (surface plasmon resonance) and SPRi (surface plasmon resonance imaging). A fusion protein (MITF-Maltose Binding Protein) was attached on the glass plate by chemical modification. An inhibitory synthetic DNA oligomer, artificially designed based on the E-box sequence, inhibited the binding of MITF and E-box. These results showed the potentials of flurorescence-based MITF protein chip as a microarray for high throughput screening (HTS) system of depigmenting agents.
Keywords
MITF (microphthalmia transcription factor); E-bok; microarray; fluoresence-based detection;
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  • Reference
1 H. Aoki and O. Moro, Involvement of microphthal-mia-associated transcription factor (MITF) in expression of human melanocortin-1 receptor (MC1R), Life Sci., 71, 2171 (2002)
2 S. H. Baek, Y. B. Shin, M. G. Kim, H. S. Ro, E. K. Kim, and B. H. Chung, Surface plasmon resonance imaging analysis of hexahistidine-tagged protein on the gold thin film coated with a calix crown derivative, Biotechnol. Biopro. Eng., 9(2), 143-146 (2004)
3 C. Levy, H. Nechushtan, and E. Razin, A new role for the STAT3 inhibitor, PIAS3: a repressor of microphthalmia transcription factor, J. Biol. Chem., 277, 1962 (2002)
4 J. M. Jung, Y. B. Shin, M. G. Kim, H. S. Ro, H. T. Jung, and B. H. Chung, A fusion protein expression analysis using surface plasmon resonance imaging, Anal. Biochem, 330, 251 (2004)
5 T. Ellenberger, D. Fass, M. Arnaud, and S. C. Harrison, Crystal structure of transcription factor E47: E-box recognition by a basic region helix-loop-helix dimer, Genes. Dev., 8, 970 (1994)
6 M. L. Bulyk, X. Huang, Y. Choo, and G. M. Church, Exploring the DNA-binding specificities of zinc fingers with DNA microarrays, Proc. Natl. Acad. Sci., 98, 7158 (2001)
7 특허: MBP chip을 이용한 MITF 전사인자 저해제의 고속탐색방법 개발(특허출원중)