• Title/Summary/Keyword: $K_{1c}$

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Purified Polysaccharide Activating the Complement System from Leaves of Diospyos kaki L. (감잎(Diospyos kaki L.)으로부터 정제한 보체계 활성화 다당류)

  • Jung, Yung-Joo;Chun, Hyug;Kim, Kyung-Im;An, Jeung-Hee;Shin, Dong-Hoon;Hong, Bum-Shik;Cho, Hong-Yon;Yang, Han-Chul
    • Korean Journal of Food Science and Technology
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    • v.34 no.5
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    • pp.879-884
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    • 2002
  • Cold and hot water fractions of Diospyros kaki were screened to determine its anti-complementary activity. Flour of Diospyros kaki leaf (250 g) was boiled at $100^{\circ}C$ for 3 h and passed through a membrane of 10 kDa molecular weight (DK-0). DK-0 was precipitated with ethanol and refluxed with methanol to obtain the crude polysaccharide (DKC). DKC-1 was isolated by ion exchange chromatography on DEAE-Toyopearl 650C, and DKC-1c was purified from DKC-1 by size exclusion chromatography on Bio gel P-60. The anti-complementary activities of DKC-1c at $1000\;{\mu}g/mL$ were 85.4 and 61.1% via whole and alternative pathways, respectively. DKC-1c was determined as a neutral polysaccharide composed of glucose (29.0 mol.%), arabinose (24.3 mol.%), and galactose (16.2 mol.%) with the molecular weight of 66.6 kDa. Results of agarose gel immunoelectrophoresis revealed DKC-1c, as a complement activator, cleaved C3 into C3a and C3b via both pathways.

Synthesis and Magnetic Properties of Body-centered-tetragonal Fe-Co Alloy (체심정방정 구조 Fe-Co계 합금상의 합성 및 그 자기적 특성)

  • Kim, K.M.;Kwon, H.W.;Lee, J.G.;Yu, J.H.
    • Journal of the Korean Magnetics Society
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    • v.27 no.4
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    • pp.129-134
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    • 2017
  • Bulk-type body-centered-tetragonal Fe-Co alloy was synthesised by utilising a conventional alloy preparation technologies, such as melting, solidification, and homogenising treatments, and its magnetic properties were investigated. In the $(Fe_{100-x}Co_x)_{1-y}C_y$ alloy, the composition range, from which single phase body-centered-tetragonal alloy (martensite phase) was obtained, was severely limited: Co content x = 2.5, and C content y = 0.062. Tetragonality(c/a) of the synthesised body-centered-tetragonal $(Fe_{97.5}Co_{2.5})_{0.938}C_{0.062}$ alloy was 1.05. Magnetocrystalline anisotropy constant ($K_1$) of the body-centered-tetragonal $(Fe_{97.5}Co_{2.5})_{0.938}C_{0.062}$ alloy was measured to be $9.8{\times}10^5J/m^3$), which was 3.1 time as high as the pure iron (${\alpha}-Fe$).

The Results and Complications of the C1-C2 Transarticular Screw Fixation Methods (축추-환추간 경관절 나사못 고정술 치료의 결과 및 합병증)

  • Choi, Jun-Woong;Yoon, Seung-Hwan;Park, Hyung-Chun;Park, Hyeon-Seon;Kim, Eun-Young;Ha, Yoon
    • Journal of Korean Neurosurgical Society
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    • v.37 no.3
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    • pp.201-206
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    • 2005
  • Objective: To evaluate the accuracy and safety of C1-C2 transarticular screw insertion, we retrospectively review surgical records and postoperative radiological findings. Methods: From January 2001 to October 2003, the C1-C2 transarticular screw fixation and posterior wiring with iliac bone grafts was performed in 16 patients. 6 patients had rheumatoid arthritis which caused cervical instability, 3 patients had os odontoideum, 3 patients had type 2 odontoid process fracture, 3 patients had traumatic transverse ligament injury and 1 patients who had been managed with C1-C2 wire fixation had psoriatic arthritis. Results: Osseous fusion was documented in 15 patients(93.8%). Only one patient was recorded screw loosening because of postoperative infection. One patient had only one screw placed because of abnormal anatomical structure, one patients was breakage of a Kirschner wire, and one screw was medial location to lateral mass of C1, but clinical results was excellent and radiological instability was not noted. Conclusion: The author's experience demonstrates that C1-C2 transarticular screw fixation with wired bone graft is a safe procedure with higher fusion rate but precaution is needed to avoid the neural damage, vertebral artery injury, and hardware failure.

Studies on the Triglyceride Composition of Some Vegetable Oils -II. Triglyceride Composition of Corn Oil- (식물유(植物油)의 Triglyceride 조성(組成)에 관한 연구(硏究) -제(第) 2 보(報) : 옥수수유(油)의 triglyceride 조성(組成)-)

  • Choi, Su-An;Park, Yeung-Ho
    • Korean Journal of Food Science and Technology
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    • v.14 no.3
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    • pp.226-231
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    • 1982
  • In order to define triglyceride compositions in fat and oil triglycerides were separated by thin layer chromatography (TLC) from corn oil, and the separated triglycerides were graduated according to each partition number(PN) by high performance liquid chromatography (HPLC) using column of ${\mu}-Bondapack\;C_{18}$ and each graduation was graduated again according to acylcarbon number by gas liquid chromatography(GLC). Fatty acid compositions were analyzed by GLC after their graduation were methylated in according to PN. The triglyceride compositions were estimated by synthesizing the above three results. The estimated triglycerides consisted of 36 kinds in corn oil. The major triglyceride compositions of sample oil were as follows: 21.5%$(C_{18:2},\;C_{18:2},\;C_{18:1})$, 17.4%$(C_{18:1},\;C_{18:2},\;C_{18:1})$, 15.4%$(C_{18:1},\;C_{18:2},\;C_{16:0})$, 11.1%$(C_{16:0},\;C_{18:2},\;C_{18:2})$, 9.0%$(C_{18:1},\;C_{18:1},\;C_{18:1})$, 8.0%$(C_{18:2},\;C_18:2},\;C_{18:2})$, 5.7%$(C_{18:1},\;C_{18:1},\;C_{16:0})$, 2.2%$(C_{16:0},\;C_{16:0},\;C_{18:2})$, 1.6%$(C_{18:2},\;C_{18:2},\;C_{18:2})$, 1.1%$C_{18:2},\;C_{18:0},\;C_{16:0})$, 1.1%$(C_{16:0},\;C_{16:0},\;C_{18:1})$.

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Identification of Ku70/Ku80 as ADD1/SREBP1c Interacting Proteins

  • Lee, Yun Sok;Koh, Hae-Young;Park, Sang Dai;Kim, Jae Bum
    • Animal cells and systems
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    • v.8 no.1
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    • pp.49-55
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    • 2004
  • In vertebrates, multisubunit cofactors regulate gene expression through interacting with cell-type- and gene-specific DNA-binding proteins in a chromatin-selective manner. ADD1/SREBP1c regulates fatty acid metabolism and insulin-dependent gene expression through binding to SRE and E-box motif with dual DNA binding specificity. Although its transcriptional and post-translational regulation has been extensively studied, its regulation by interacting proteins is not well understood. To identify cellular proteins that associate with nuclear form of ADD1/SEBP1c, we employed the GST pull-down system with Hela cell nuclei extract. In this study, we demonstrated that Ku proteins interact specifically with ADD1/SREP1c protein. GST pull-down combined with peptide sequencing analysis revealed that Ku80 binds to ADD1/SREBP1c in vitro. Additionally, western blot analysis showed that Ku70, a heterodimerizing partner of Ku80, also associates with ADD1/SREBP1c. Furthermore, co-transfection of Ku70/Ku80 with ADD1/SREBP1c enhanced the transcriptional activity of ADD1/SREBP1c. Taken together, these results suggest that the Ku proteins might be involved in the lipogenic and/or adipogenic gene expression through interacting with ADD1/SREBP1c.

ROBUSTLY SHADOWABLE CHAIN COMPONENTS OF C1 VECTOR FIELDS

  • Lee, Keonhee;Le, Huy Tien;Wen, Xiao
    • Journal of the Korean Mathematical Society
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    • v.51 no.1
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    • pp.17-53
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    • 2014
  • Let ${\gamma}$ be a hyperbolic closed orbit of a $C^1$ vector field X on a compact boundaryless Riemannian manifold M, and let $C_X({\gamma})$ be the chain component of X which contains ${\gamma}$. We say that $C_X({\gamma})$ is $C^1$ robustly shadowable if there is a $C^1$ neighborhood $\mathcal{U}$ of X such that for any $Y{\in}\mathcal{U}$, $C_Y({\gamma}_Y)$ is shadowable for $Y_t$, where ${\gamma}_Y$ denotes the continuation of ${\gamma}$ with respect to Y. In this paper, we prove that any $C^1$ robustly shadowable chain component $C_X({\gamma})$ does not contain a hyperbolic singularity, and it is hyperbolic if $C_X({\gamma})$ has no non-hyperbolic singularity.

Synthesis and Anti Bacterial and Anti-ulcer Evaluation of New S-mannich Bases of 4,6-diaryl-3,4-dihydropyrimidin-2(1H)-thiones

  • Kodhati, Venkateshwarlu;Vanga, Malla Reddy;Yellu, Narsimha Reddy
    • Journal of the Korean Chemical Society
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    • v.57 no.2
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    • pp.234-240
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    • 2013
  • The synthesis of title compounds were accomplished by synthetic sequence shown in Scheme 1. Chalcones on cyclocondensation with thiourea in ethanol and potassium hydroxide under reflux yielded the respective dihydropyramidin-2(1H)-thiones. Each of the dihydropyrimidin thiones was, then subjected to the Mannich condensation in alkaline medium using three different secondary amines, viz., dimethylamine, diethylamine and morpholine to obtain a new series of S-Mannich bases. All the synthesised compounds ($C_1-C_{15}$) were evaluated for their antiulcer and antibacterial activities. Compounds $C_4$, $C_5$, $C_6$, $C_{14}$ and $C_{15}$ exhibited relatively more potent antiulcer activity but not comparable to the standard; Omeprazole, while $C_1$, $C_2$, $C_3$ and $C_{13}$ were moderate in activity at 100 mg/kg p.o. All the compounds ($C_1-C_{15}$) showed mild to moderate activity against both Gram-positive (S.aureus, L.delbrueckii) and Gram-negative (P.vulgaris, E.coli) bacteria. Amongst the compounds tested, only $C_6$, $C_9$, $C_{12}$ and $C_{15}$ were found to be potent.

Survey on Storage Temperature of Domestic Major Chilled Foods in Refrigerator (우리나라 주요 냉장판매식품의 보관온도 실태 조사)

  • Lee, Yu-Si;Ha, Ji-Hyoung;Park, Ki-Hwan;Lee, Sook-Yeon;Choi, Youn-Ju;Lee, Dong-Ho;Park, Sun-Hee;Moon, Eun-Sook;Ryu, Kyung;Shin, Hyoung-Soo;Ha, Sang-Do
    • Journal of Food Hygiene and Safety
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    • v.23 no.4
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    • pp.304-308
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    • 2008
  • We investigated temperature management for refrigerated foods in large discount markets, department stores, and convenience stores. Eleven stores in the Seoul area were used to examine temperature maintenance for frozen and refrigerated foods, including soybean curds (tofu), fish surimi, mook, wet noodles, kimbab, and salads. The surficial and central temperatures of foods in a refrigerator and a freezer were examined. While the difference between the highest ($7.5^{\circ}C$) and the lowest ($6.4^{\circ}C$) temperature spaces in a refrigerator was $1.1^{\circ}C$, the corresponding difference between the highest ($8.9^{\circ}C$) and lowest ($7.5^{\circ}C$) food surface temperatures was $1.4^{\circ}C$. The average temperatures of both chilled food surfaces and refrigerator spaces were $7^{\circ}C$ (max. $22.9^{\circ}C$) and 8.2 degrees C (in. $-0.4^{\circ}C$), respectively. The temperatures of chilled food surfaces were $6.1{\times}10.6^{\circ}C$ for soybean curds, $6{\sim}12.3^{\circ}C$ for fish paste, $2.3{\times}18.2^{\circ}C$ for kimbab, $4.6{\times}12.2^{\circ}C$ for salads, $3.4{\times}12.6^{\circ}C$ for wet noodles, and $7.1{\times}19^{\circ}C$ for mook. Our results indicate that chilled foods require careful management for storage at temperatures below $10^{\circ}C$ with careful recording of the warmest area of the refrigerator.

Theoretical Study on the Geometric Property of Higher Fullerene C90 (Higher Fullerene C90의 기하학적 성질에 대한 이론적 연구)

  • Kwak, K.D.;Ha, Y.S.;Seo, M.R.
    • Applied Chemistry for Engineering
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    • v.10 no.6
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    • pp.881-884
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    • 1999
  • We have studied the other unit which is partial element in fullerene $C_{90}$. The energy of the higher fullerene increased by 5.6 kcal/mol for each $1.0{\beta}$ of planarity for the other units and increased by 11.0 kcal/mol for each $2C_1$ other unit at RHF/AM1 method. Therefore, we have known that the stable $C_{90}$ isomer do not have $C_1$ unit in other unit and have small planarity. The confirmable $C_{90}$ isomers by experiment satisfies these two conditions.

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