• Journal of Microbiology
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• v.44 no.1
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• pp.42-49
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• 2006

In this study, we describe our newly-developed sensitive two-stage PCR procedure for the detection of 13 common mycoplasmal contaminants (M. arthritidis, M. bovis, M. fermentans, M. genitalium, M. hominis, M. hyorhinis, M. neurolyticum, M. orale, M. pirum, M. pneumoniae, M. pulmonis, M. salivarium, U. urealyticum). For primary amplification, the DNA regions encompassing the 16S and 23S rRNA genes of 13 species were targeted using general mycoplasma primers. The primary PCR products were then subjected to secondary nested PCR, using two different primer pair sets, designed via the multiple alignment of nucleotide sequences obtained from the 13 mycoplasmal species. The nested PCR, which generated DNA fragments of 165-353 bp, was found to be able to detect 1-2 copies of the target DNA, and evidenced no cross-reactivity with the generated DNA of related microorganisms or of human cell lines, thereby confirming the sensitivity and specificity of the primers used. The identification of contaminated species was' achieved via the performance of restriction fragment length polymorphism (RFLP) coupled with Sau3AI digestion. The results obtained in this study furnish evidence suggesting that the employed assay system constitutes an effective tool for the disagnosis of mycoplasmal contamination in cell culture systems.

• Bulletin of the Korean Mathematical Society
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• v.57 no.3
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• pp.803-813
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• 2020

Let M be a finitely generated G-projective R-module over a PVMD R. We prove that M is projective if and only if the canonical map θ : M⨂_R M^∗ → Hom_R(Hom_R(M, M), R) is a surjective homomorphism. Particularly, if G-gldim(R) ⩽ ∞ and Extⁱ_R(M, M) = 0 (i ⩾ 1), then M is projective.

• Animal cells and systems
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• v.4 no.3
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• pp.293-297
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• 2000

The present investigation was performed to elucidate the effect of pretreatment with low dose ultraviolet radiation (UV) and ethyl methanesulfonate (EMS) on cell survival by trypan blue dye exclusion method and plasma membrane glycoconjugates by lectin-cytochemistry in sarcoma 180 (S180) cells. Pretreatment with 2 J/$m^2$ UV or 2 mM EMS increased the percentage of survival of cells subsequently treated with high dose UV (10 or 20 J/$m^2$) or EMS (10 or 20 mM), respectively. Staining intensity of concanavalin A (Con A) of the cells pretreated with 2J/$m^2$ UV or 2 mM EMS and subsequently treated with 10 or 20 mM EMS was stronger than that of the cellstreated with 10 or 20 mM EMS. These results suggest that there is an adaptive response on cell survival to EMS or UV in S180 cells. And the results show a change in mannose-containing glycoconjugates of plasma membrane in S180 cells pretreated with EMS or UV and subsequently treated with EMS.

• BMB Reports
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• v.44 no.8
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• pp.506-511
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• 2011

Mammalian Target of Rapamycin (mTOR) is a serine/threonine kinase and that forms two multiprotein complexes known as the mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2). mTOR regulates cell growth, proliferation and survival. mTORC1 is composed of the mTOR catalytic subunit and three associated proteins: raptor, mLST8/$G{\beta}L$ and PRAS40. mTORC2 contains mTOR, rictor, mLST8/$G{\beta}L$, mSin1, and protor. Here, we discuss mTOR as a promising anti-ischemic agent. It is believed that mTORC2 lies down-stream of Akt and acts as a direct activator of Akt. The different functions of mTOR can be explained by the existence of two distinct mTOR complexes containing unique interacting proteins. The loss of TSC2, which is upstream of mTOR, activates S6K1, promotes cell growth and survival, activates mTOR kinase activities, inhibits mTORC1 and mTORC2 via mTOR inhibitors, and suppresses S6K1 and Akt. Although mTOR signaling pathways are often activated in human diseases, such as cancer, mTOR signaling pathways are deactivated in ischemic diseases. From Drosophila to humans, mTOR is necessary for Ser473 phosphorylation of Akt, and the regulation of Akt-mTOR signaling pathways may have a potential role in ischemic disease. This review evaluates the potential functions of mTOR in ischemic diseases. A novel mTOR-interacting protein deregulates over-expression in ischemic disease, representing a new mechanism for controlling mTOR signaling pathways and potential therapeutic strategies for ischemic diseases.

• BMB Reports
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• v.29 no.6
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• pp.569-573
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• 1996

Clinical strains of Mycobacterium tuberculosis, M. terrae complex, M. gordonae, M. avium-intracellulae complex, and M. fortuitum from Korean patients were isolated and analyzed by comparing large restriction fragment (LRF) patterns produced by digestion of genomic DNA with infrequent-cutting endonucleases like AsnI and XbaI. and pulsed-field gel electrophoresis (PFGE). Three M. tuberculosis, two M. terrae complex, two M. gordonae, two M. avium-intracellulae complex, and two M. fortuitum strains were compared by using AsnI and XbaI. and this allowed easy visual separation of all epidemiologically unrelated strains. PFGE exhibits different DNA restriction patterns which are easy to compare. Genome size of the strains roughly ranged from 3020 to 3335 kb. The LRF patterns are useful for epidemiologic studies of tuberculosis with regard to drug resistance.

• Proceedings of the Korean Information Science Society Conference
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• 2012.06d
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• pp.230-232
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• 2012

M2M 서비스는 센서 네트워크의 데이터 수집 및 제어를 넘어서는 새로운 타입의 서비스이다. M2M 서비스는 홈 네트워크 디바이스, 사용자의 스마트 디바이스, 개인 컴퓨터 등을 이용하여 스마트 환경의 감시, 관리, 그리고 제어 등을 제공하는 새로운 서비스 타입이다. 오늘날 M2M 서비스를 제공하기 위한 연구들이 진행되고 있는데, 이를 제공하기 위한 플랫폼 연구는 아직 많이 부족한 현실이다. 따라서 본 논문은 서비스 오버레이 네트워크를 이용하여 M2M 서비스를 제공하기 위한 플랫폼 구조를 제안한다. 또한 다양한 M2M 서비스들을 조합하여 새로운 서비스를 사용자에게 제공할 수 있는 서비스 컴포지션 방법 및 절차를 제안한다. 서비스 오버레이 네트워크 플랫폼을 이용한 M2M 서비스 플랫폼은 사용자에게 향상된 서비스 기능, 범용성, 호환성, 그리고 지능화 및 능동적 기능을 제공할 수 있다.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2006.05a
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• pp.345-346
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• 2006

In the future, a machine will be more improved in the form of advanced concept with collaborative ability in M2M(Machine to Machine, Mobile to Machine) environment for u-Manufacturing system. This paper tried to standardize M2M and design advanced concept machine. The M2M is front-end system for implementing autonomous ubiquitous environment. The advanced machine in M2M will be a collaborative machine with knowledge-evolutionary ability such as u-Machine(Ubiquitous machine), Vortal(Vertical Portal) machine and P2P(Peer to Peer) machine. Such advanced concept machines will be the key subject for M2M cooperation.

• Journal of the Korean Society for Heat Treatment
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• v.6 no.2
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• pp.107-116
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• 1993

• 대한핵의학회:학술대회논문집
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• 1989.06a
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• pp.141.1-141.1
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• 1989

• The Korean Journal of Zoology
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• v.33 no.1
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• pp.103-107
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• 1990

lgM-like immunoglobulin was purified from the immune serum of M albus which immunized with bovine Serum albumin(BSA) as an antigen(Ag) and characterized. The Ag-specific antibody activity of the immune serum was increased after the immunization. The purified lgM-like immunoglobulin had a tetrameric structure which had a molecular weight of 800 kD and the monomer of IgM-like Ig had a mass of 199 kD which was composed of two heavy chains (Mol. wt. 70 kD) and iwo light chains (Mol. wt. 29.5 kD). The IgM-like Ig showed hemaggluti nating activity to mammalian RBC slightly.