Animal cells and systems

The Korean Society for Integrative Biology (한국통합생물학회)
권호 표지

Effect of Low Dose Mutagens on Adoptive Response and Plasma Membrane Glycoconjugates in Sarcoma 180 Cells

  • Published : 2000.09.01
Download PDF
⟨ Previous Next ⟩

Abstract

The present investigation was performed to elucidate the effect of pretreatment with low dose ultraviolet radiation (UV) and ethyl methanesulfonate (EMS) on cell survival by trypan blue dye exclusion method and plasma membrane glycoconjugates by lectin-cytochemistry in sarcoma 180 (S180) cells. Pretreatment with 2 J/$m^2$ UV or 2 mM EMS increased the percentage of survival of cells subsequently treated with high dose UV (10 or 20 J/$m^2$) or EMS (10 or 20 mM), respectively. Staining intensity of concanavalin A (Con A) of the cells pretreated with 2J/$m^2$ UV or 2 mM EMS and subsequently treated with 10 or 20 mM EMS was stronger than that of the cellstreated with 10 or 20 mM EMS. These results suggest that there is an adaptive response on cell survival to EMS or UV in S180 cells. And the results show a change in mannose-containing glycoconjugates of plasma membrane in S180 cells pretreated with EMS or UV and subsequently treated with EMS.

Keywords

References

  1. Bhushan A, Abramson R, Chiu J-F, and Tritton TR (1992) Expression of c-tos in human and murine multidrug-resistant cells. Mol Pharmacol 42: 69-74
  2. Bradley G, Juranka PF, and Ling V (1988) Mechanism of multidrug resistance. Biochim Biaphys Acta 948: 87-128 https://doi.org/10.1016/0304-419X(88)90006-6
  3. Cole SPC, Sparks KE, Fraser K, Loe OW, Grant CE, Wilson GM, and Deeley RG (1994) Pharmacological characterization of multidrug resistant MRP-transfected human tumor cells. Cancer Res 54: 5902-5910
  4. Devary Y, Gottlieb RA, Smeal T, and Karin M (1992) The mammalian ultraviolet response is triggered by activation of Src tyrosine kinases. Cell 71: 1081-1091 https://doi.org/10.1016/S0092-8674(05)80058-3
  5. Ford JM and Hait WN (1990) Pharmacology of drugs that alter multidrug resistance in cancer. Pharmacol Rev 42: 155-199
  6. Gottesman MM and Pastan I (1993) Biochemistry of multidrug resistance mediated by the multidrug transporter. Annu Rev Biochem 62: 385-427 https://doi.org/10.1146/annurev.bi.62.070193.002125
  7. Holbrook NJ and Fornace AJ Jr (1991) Response to adversity: molecular control of gene activation following genotoxic stress. New Biol 3: 825-833
  8. Juliano RL and Ling V (1976) A surface glycoprotein modulating drug permeability in Chinese hamster ovary cell mutants. Biochim Biophys Acta 455: 152-162 https://doi.org/10.1016/0005-2736(76)90160-7
  9. Lee JH, Oh KS, Lee DW, Shin EJ, and Um KI (1998) The effect of pretreatment with various mutagens on glycoconjugates of plasma membrane in HeLa cells. Environ Mutagens Carcinogens 18: 116-122
  10. Lundh B, Brochstedt U, and Kristensson K (1989) Lectinbinding pattern of neuroepithelial and respiratory epithelial cells in the mouse nasal cavity. Histochem J 21: 33-43 https://doi.org/10.1007/BF01002469
  11. Moon YS, Kim EY, and Um KI (1993) Effect of pretreatment with various mutagens on cell survival and DNA repair in Chinese hamster ovary cells. Environ Mutagens Carcinogens 13(2): 83-91
  12. Perera MI, Um KI, Greene MH, Waters HL, Bredberg A, and Kraemer KH (1986) Hereditary dysplastic nevus syndrome: lymphoid cell ultraviolet hypermutability in association with increased melanoma susceptibility. Cancer Res 46: 1005-1009
  13. Samson L and Cairns J (1977) A new pathway for DNA repair in Escherichia coli. Nature 267: 281-283 https://doi.org/10.1038/267281a0
  14. Samson L and Schwartz JL (1980) Evidence for an adaptive DNA repair pathway in CHO and human skin fibroblast cell lines. Nature 287: 861-863 https://doi.org/10.1038/287861a0
  15. Shadley JD and Dai G (1992) Cytogenetic and survival adaptive response in G1 phase human lymphocytes. Mutation Res 265: 273-281 https://doi.org/10.1016/0027-5107(92)90056-8
  16. Shadley J D and Dai G (1993) Evidence that adaptive response of human lymphocytes to ionizing radiation acts on lethal damage in nonaberrant cells. Mutation Res 301: 171-176 https://doi.org/10.1016/0165-7992(93)90074-6
  17. Terasima T, Takabe Y, Kasumata I, Watanabe M, and Umezawa H (1972) Effect of bleomycin on mammalian cell survival. J Nat Cancer Inst 49: 1093-1100