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Optimization of Submerged Culture Conditions for the Growth Increase of Ginseng Adventitious Root Containing Germanium (게르마늄 함유 인삼 부정근의 생장 증대를 위한 액체배양 조건의 최적화)

  • Chang, Eun-lung;Oh, Roon-II
    • Journal of Ginseng Research
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    • v.33 no.2
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    • pp.143-148
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    • 2009
  • This study was carried out to detennine the optimal submerged culture conditions for the growth increase of ginseng adventitious roots containing germanium by means of a fractional factorial design with four factors and three levels, using the response surface methodology (RSM). The ginseng (Panax ginseng CA. Meyer) adventitious roots were induced by plant growth regulators and cultured in a liquid SH medium. The effects of various $GeO_2$ and phosphoric acid ($H_3P0_4$) concentrations in the medium, $GeO_2$ addition time and the pH of the medium on the fresh weight of the ginseng adventitious roots were investigated. The optimum pH of the medium and the phosphoric acid concentration detennined by the partial differentiation of the model equation were 4.7 and 6.0 roM, respectively. The predicted optimal $GeO_2$ concentration was 10 ppm and the $GeO_2$ addition time did not affect the growth of ginseng adventitious roots. Under these conditions, the growth of the ginseng adventitious root containing germanium was predicted to be 2.47 g.

Chromosomal Abnormalities in Human Oocytes Fail to Fertilize after Insemination In Vitro (수정에 실패한 인간 난자에 있어서의 염색체의 수의 이상)

  • Son, Weon-Young;Lee, Kyung-Ah;Park, Sang-Hee;Han, Sei-Yul;Yoon, Tae-Ki;Jung, Hyung-Min;Kwak, In-Pyung;Cha, Kwang-Yul
    • Clinical and Experimental Reproductive Medicine
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    • v.22 no.2
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    • pp.203-210
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    • 1995
  • Many oocytes fail to fertilize and cleave in vitro and many embryos transferred back to uterus fail to implant or maintain implantation. Chromosomal abnormalities in the male and female gametes may contribute to this loss. The higher incidence of meiotic chromosomal abnormalities bas been found in oocytes than in sperm. The wide range of incidence of chromosomal abnormalities in unfertilized oocytes has been reported in human IVF program (26-63%). However, factors affecting chromosomal abnormalities are not well understood. The present study has been conducted to investigate effects of the method for ovarian hyperstimulation, women's age, and the number of oocytes retrieved per patients on the incidence of numerical chromosomal abnormalities. Five hundred eighty four unfertilized metaphase II oocytes were subjected to chromosomal analysis. Included unfertilized oocytes were from 220 patients (mean $age=32.7{\pm}3.0$) and three hundred thirty oocytes were legible for analysis. Two hundred fourty five oocytes out of 330 (73.3%) were normal, while 38 (11.5%) were hyperploidy, 35 (10.6%) were hypoploidy, and 12 (3.6%) were diploidy. Significant difference in chromosomal abnormalities was not found between two patient groups stimulated by follicular stimulating hormone/human menopausal gonadotrophin (FSH/HMG) (25.9%) and gonadotrophin-releasing hormone agonist/follicular stimulating hormone/human menopausal gonadotrophin (GnRHa/FSH/HMG) (28%). There was a tendency of increasing chromosomal abnormalities in unfertilized oocytes from older patients (<30 yrs: 20.3%, 30-34yrs: 26.9%, >34 yrs: 35.3%). The number of oocytes retrieved per patient had no effect the incidence of chromosomal abnormalities (1-5: 31. 4%, 6-10: 29.8%, 11-15: 28.6%, > 15: 16.5%). These results from the present study suggest that the chromosomal abnormalities observed in the unfertilized oocytes has not affected by the stimulation methods, patient's age, and the number of oocytes retrieved per patients.

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A Study on the Student Guidance at Home Management House (가정관리실습관에 있어서의 학생지도에 관한 연구 - 서울 시내모여자대학교대상-)

  • 김복길
    • Journal of the Korean Home Economics Association
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    • v.12 no.34
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    • pp.679-697
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    • 1974
  • I. Preface A. Establishment. There are many psycological and physiological difficulties that students have made practical application to a building site of 600 pyung and a structure of 65 pyung each sixteen students in the Home Management House, but there exists problem in students apprentice and management of professors. B. Purpose of study This research aim to seek a high degree of efficiency approach in investigating, studying and analyzing factors of the problem in Home Management which have been faced in women colleges in seoul. C. Content of study. This study is aim at finding out the problems including facilites, system, guiding principle, working bottleneck and administrative problem in making practical application of Home Management. II. A plan and method of study A. This subject of statiscal research is 616 women students in senior year from september 27 1970 to October 31 1974. The rate of collection of questionnaire is 100 percent. B. The Method of study is devised through refering documents, interview with students, questionnaire and observation. III. Result of study Of the total of 29 university in seoul, 24 Universities have its Course of Home Economic among which only 12 have Home Management for making practical application. Home Management House for making practical application is located mainly in campus but some of them exceptimally are in outside university. Students practice mainly at civilzed resident, apartment, bangalow so forth, and demention of which occupies approxiamtely 50-600 pyung (land) and 20-70 pyung (Building). A member with 4 or 20 students make practical application from 10 days to 3 months. A member consists of students and assistant teacher, and practice site classifies into living school for Professors living, and another school for Professors and assistant teachers. Answer to a questionnaire. 616 students answer to a questionnaire. 49 Perfect of 616 students like to have a member with 12 students like to have a member with 12 students, 38 percent student a member with 16 students. About the period of making practical About the period of making practical application, 76 percent students like to have two or three week period. 19 percent students think it a short period and 4 percent students think it a long period. There is none who thinks 25 day period day period to be short. About making practical application, 66 percent of 616 students feel uneasy, 40 percent students feel lack of sleep and tired. Students take a pleasure in having a conversation with their friends, inviting their mother, having dinner-party and birthday party. 77 percent students want to invite their father and 67 percent students want to invite their boy-friends. It is hard to have a good personal relation about the great the result of practice, students acknowledge themselves that they have learned about Cautiousness and they are sure of good Home Manager. There are 9 difficult problems during the period. IV. Summary discussion a. Many problems come from having a number of students. b. Students feel tired with their practice if over three weeks. No Matter how good a plan is, it useless if students can't adapt themselves to it. Suggestions 1) it is good to have a member with 5 or 6 students, and it is suitable to have two or three week period, and to have an area of 20 or 30 pyung. 2) it's proposed a profess or to live together in making practical application of Home Management 3) it is proposed a professor to have a sure T.O. and to be treated better.

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Personality Trait in Panic Disorder Patient and Its Clinical Significance (공황장애 환자의 성격특성과 임상적 의의)

  • Chae, Young-Lae;Lee, Chung-Tai;Kim, Bo-Yeon;Lee, Sung-Pil;Hong, Seung-Chul;Kim, Chong-Woo;Keh, Yoon-Jung
    • Korean Journal of Psychosomatic Medicine
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    • v.3 no.2
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    • pp.139-146
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    • 1995
  • Forty patients meeting DSM-III-H criteria for panic disorder and 51 normal controls were assessed with the Personality Diagnostic Questionnaire-Revised(PDQ-R), a self-rating scale designed to assess Axis II personality disorders and traits. Results replicated previous findings of a preponderance of dependent, avoidant, and histrionic features. But our result showed other features such as paranoid, schizotypal, borderline, and antisocial traits also. Patients were divided into two groups according to the severity of their personality traits(high or low). These groups were compared in various panic symptomatology and SCL-90-R. None of the specific symptom dimensions in panic disorder, i.e. panic, anxiety, agoraphobia, social impairment, or chronicity was different between groups. Rather, high personality trait groups were found to have significantly more symptomatology in SCR-90-R than low personality groups. Result indicated that patients exhibiting a greater number of personality traits were also significantly more symptomatic. The results suggested a possible link beteween panic disorder and personality disorder. And, general factors such as depression, social or interpersonal sensitivity might provide a much better explanation of personality disorders in panic patients.

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Factors Affecting the Choice of Medical Care Use by the Poor (저소득층의 의료 이용과 욕구 미충족에 영향을 미치는 요인)

  • Kim, Jin-gu
    • Korean Journal of Social Welfare Studies
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    • no.37
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    • pp.5-33
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    • 2008
  • This study examines the use of medical care by the poor through analysing KNHANES III databases, and the focus of the study is on under-satisfaction of medical needs and the impact of the medicare system. The results of analysis are summerized as follows; the poor had generally suffered from poor health condition, and did not have economic resoure to satisfy the medical needs. But, the beneficiaries of the medicare used much more medical care than non-poor. The result of logistic regression suggest that the medicare affected significantly on increase of uses. Consquently, the medicare system effectively made up the lack of economic resoure of the poor. However, the Medicare did not sufficient to satisfy all the medical needs of the poor. Over 20% of the poor had experinced the abandonment of meical care uses, "the lack of econmic resource" was most important reason. The result of logistic regression suggest that all the poor such as Medicare I and Medicare II beneficiaries, and near-poor class had much more probabilities of giving up the use of medical care than non-poor. It is necessary to raise up the benefit level of the current medicare system such as the reduction of non-secured medical cost, the alleviation of user's burden etc.

A Study on Developing Korean Naval Forces by Examining How Japan built their Maritime Self-Defense Force: Focusing on the 'eight (艦)-eight (機) fleet system' (일본 해상자위대 전력건설 재조명을 통한 한국 해군력 건설논리 발전방향 연구: '8함(艦) 8기(機) 체제'를 중심으로)

  • Ryu, Jae-hak
    • Maritime Security
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    • v.2 no.1
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    • pp.43-70
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    • 2021
  • This study started from questioning our general view that the Japan Maritime Self-Defense Force (JMSDF) is a natural result of Japan being a prosperous and island country. Japan's naval power collapsed after World War II. The initial power of the JMSDF, founded in 1954, was very small and not different from the Republic of Korea Navy (ROKN), mostly consisting of ships given by or borrowed from the US. Although growing into the world's second-largest economy and the geographical characteristics of an island nation acted as an opportunity to increase naval forces, there were also difficulties in the early stage, such as a temporary severance with the Japanese Navy, strategic thinking centered on land forces, and an economic crisis caused by the oil crisis. As a result, the JMSDF has become a world-class naval power and is developing into a four helicopter carrier operating system, which may change the basic concept of exclusive defense. There are various factors behind the growth of Japan's naval power, but this study explores how to develop Korean naval forces by analyzing how Japan developed their escort fleet, the core of the JMSDF, focusing on the eight (艦)-eight (機) fleet system, which was the basic logic behind building the JMSDF.

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The American Route of the Reconciliation between Work and Family (미국 중산층 여성 일-가정양립 경로의 역사적 형성과정에 관한 연구)

  • Choi, Seong Eun;Yang, Jae-jin
    • 한국사회정책
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    • v.23 no.3
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    • pp.79-108
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    • 2016
  • This study is focused on the historical route in which reconciliation between work and family has been operating in the United States whose welfare standards are low, by using analytic narratives, from late 19th century to early 20th century. The first step saw an increase in the social advancement of unmarried women due to the increase in employment in the occupation of white-collar, as well as the increase of women in the Academy and in educational institutions. In the second step, the social advancement of married women was increased by the enforcement of the New Deal, consumption capitalism, and World War II. In the third step, the sphere of highly-educated women was expanded to a professional one through active measures aimed at gender equality, and the women's liberation movement of the 1960s took place. The United States' path model was completed in the fourth step. This study show that the most important factors have a significant influence to complete route of the American's Route were individual efforts of women (capacity building through the academic and the powerful women's movement) and the individual employment relationship in labor market. This study shows that individual efforts of women, especially in building capacity through the academic and the powerful women's movement, and the labor market, whose individual employment relationship is well-developed, have a significant influence on completing the route of the highly educated middle-class women in America.

Examining Access Mode Choice Behavior of Local Metropolitan High-Speed Rail Station - A Case Study of Dong-Daegu Station - (고속철도 지방대도시 정차역의 연계교통수단 선택모형 구축에 관한 연구 - 동대구역을 사례로 -)

  • Kim, Sang Hwang;Kim, Kap Soo
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.26 no.4D
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    • pp.565-571
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    • 2006
  • This study aimed to analyze access mode choice behavior for KTX Passengers. To fulfill the aims of this study, Dong-Daegu Station was selected as a station for a case study. This study takes place in two stages. These are (i) descriptive statistical analysis of transportation status before and after introduction of the KTX, (ii) empirical model estimation for analyzing access mode choice behavior. This study makes use of the data from travel survey from Daegu metropolitan area. The main part of the survey was carried out in the KTX Dong-Daegu station. The data was collected from a sample of 1,800 individuals. The survey data includes the information on travel from Dong-Daegu station to Seoul. From descriptive statistical analysis of transportation status before and after introduction of the KTX, it is found that revealed demand of the KTX is lower than that expected. Moreover, it is found that the low demand of the KTX stems from high cost for the KTX itself and inconvenience( including travel time and cost) of access mode. In order to analyze mode choice behavior for accessing Dong-Daegu station, multinomial logit model structure is used. For the model specification, a variety of behavioral assumptions about the factors which affect the access mode choice, were considered. From the empirical model estimation, it si found that access travel time and access travel cost are significant in choosing access mode. Given the empirical evidence, we see that improvement of access transportation system for Dong-Daegu station is very important for enhancing the use of KTX.

A Study on Microbial Community Diversity and Antibiotic Resistance in Public Waters in Gwangju (광주지역 공공수역의 미생물 군집 다양성 및 항생제 내성에 관한 연구)

  • Sun-Jung Kim;Ji-Young Park;Seung-Ho Kim;Min-Hwa Lim;Ji-Yong Yu;Kyu-Sung Han;Se-Il Park;Gwangyeob Seo;Gwangwoon Cho
    • Journal of Environmental Health Sciences
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    • v.50 no.2
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    • pp.93-101
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    • 2024
  • Background: As pollutants caused by non-point sources flow into rivers, river water quality monitoring for fecal pollution is becoming increasingly important. Objectives: This study was conducted to investigate the distribution of microbial communities in the Yeongsangang River water system and sewage treatment plants in Gwangju and to evaluate their antibiotic resistance. Methods: In the experiment, samples were distributed to five selective media at each point and then cultured for 18 to 24 hours. When bacteria were observed, they were sub-cultured by size and shape and identified using MALDI-TOF MS equipment. When identification was completed, 17 types of antibiotic susceptibility tests were performed using VITEK II equipment, focusing on gram-negative dominant species among the identified strains. Results: During the study period, a total of 266 strains were isolated from 39 samples. Gram-positive bacteria were 37 strains in four genera, or 13.9% of the total, and Gram-negative bacteria were 229 strains in 23 genera, or 86.1% of the total. Antibiotic susceptibility testing of 23 strains, the major dominant species, showed that one strain (4.3%) was resistant to only one antibiotic, and two strains (8.7%) were 100% susceptible to the 17 antibiotics tested. The other 20 strains (87.0%) were multidrug resistant bacteria resistant to two or more antibiotics. There were various types of multidrug resistance. Among them, penicillin and cephalosporin series showed the highest resistance. Conclusions: Based on the results of this study, it was found that the bacterial community structure changed according to regional and environmental factors, and it was judged that continuous research such as genetic analysis of antibiotic-resistant bacteria present in natural rivers is necessary.

한국 청소년의 약물남용과 비행행위

  • 김성이
    • Korea journal of population studies
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    • v.11 no.2
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    • pp.54-66
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    • 1988
  • I. Introduction Since the 1970's drug abuse among young people has increasingly become a social problem in Korea. In the 1980's, drug abuse, especially glue sniffing, has become the cause of many unfortunated incidents resulting in harm to others as well as the abusers themselves. Taking into consideration of the seriousness of this problem, the Republic of Korea National Red Cross initiated a nation-wide research programme, to understand the present situation and to raise the level of public awareness. The goal of this research was to begin a nation - wide campaign against drug abuse. The research team was composed of the Advisary Committee members and the staff of the Youth Department of the Republic of Korea National Red Cross. The data were collected in February 1988 with the collaboration of the staff and volunteers in the local Chapters. The respondents were allocated nation-wide by the quota sampling method. The questionnaires were distributed to the respondents in three groups :2, 700 to junior and senior high school students, 605 to working youths, and 916 to delinquent youths. A total of 4, 221 questionnaires were collected. II. Characteristics of the Respondents The respondents in each group were selected evenly from rural and urban areas. The general characteristics of the respondents can be described as follow: in case of students, the proportions between male and female respondents, and between senior high school and junior high school students were almost evenly distributed. In case of working youths, the proportion of females (80.5%) was higher than those of the students and the delinquents groups. Delinquent youths were defined as those currently being under custody of the centers for juvenile delinquents. Of this number, 38.8% and 68.2% were junior and senior high school drop-outs respectively. The majority of them (92.6%) were male. As for the family background of the respondents, the proportion of those residing in poverty - stricken areas, and the proportion of those from broken families were higher in case of working youths and delinquent youths than those in case of students. III. Present Patterns of Drug Abuse The following summarizes the presents of drug abuse, as tabulated from the results of the survey. 1. Smoking The percentage of youths who smoke was 36% in the student group, 32% m the working youths group, and 94.4% in the delinquent youths group. 2. Alcohol 50.3% of students, 71.6% of working youths, and 93.3% of delinquent youths has experienced drinking alcohol beverages. 3. Tonic: non - alcoholic, caffeinated beverages popular in Korea and Japan The percentage of those who have used tonic at least once was over 90% in all of the three groups. 4. Sedative About 70% of each group has used sedative with the proportion of working youths use higher than those in other groups. 5. Stimulants Those who have used stimulants comprised around 15% in each group. 6. Tranquilizers Somewhat less than 5% of students and working youths, and 28% of delinquent youths, have used tranquilizers. 7. Hypnotics The users of hypnotics amounted to 0.4% of students, 2.6% of working youths and 7.1% of delinquent youths. 8. Marihuana Those who have used marihuana indicated 0.7% of students, 0.8% of working youths, and 13% of delinquent youths. 9. Glue-sniffing The percentage of glue-sniffing was 3.7%, 5% in the students group and in the youths group respectively, but the proportion was unusually high, at 40.7% in the delinquent youths group. From the results of the survey the present situation of drug abuse in Korea can be summarized as follows: 1. A high percentage of Korean youths have experienced smoking cigarettes and drinking alcoholic beverages. 2. Tonics (non - alcoholic, caffeinated beverages), antipyretic analgesics and stimulants quite regularly used. 3. Tranquilizers, hypnotics, marihuana and glue-sniffing are more widely used among delinquent youths than the other youths. From this fact, there exists a correlation between drug abuse and juvenile delinquency. IV. Time-series Analysis of the First Experience of Drug Abuse and Deviant Behaviour The respoundents were asked when they were first exposed to drugs and when they committed deviant acts. By calculating the average age of each experience, the following pattern was found (See Figure 1). Youths are first exposed to drugs by abuse of tonic(non - alcoholic, caffeinated beverages). At the age of 13, they amoke cigarettes, the use of antipyretic analgesics begins at 14 year old, while at the age of 15, they use tranquilizers, and at 16 hynotics. The period of drug abuse which starts from drinking caffeinated beverages and smoking cigarettes and ends in the use of hypnotics takes about three years. During this period, other delinquent behaviours begin to surface, that is, at the age of 13 when smoking cigarettes begins, the delinquent behaviour pattern starts with truancy. Next, they start taking money from others by using physical force. Prior to the age of 15, they are suspended from school, become hostile to adults, begin running away from home, and start using stimulants and alcohol. Soon they become involved even in glue-sniffing and in the use of marihuana. At the age of 15, they begin to see adult videos and carry weapons. Sexual promiscuity and usage of tranquilizers follows the viewing of adult videos. Consequently, by the time they reach the age of 16, they visit drinking establishments, and are picked up by police for committing delinquent acts. And finally, they come to use hypnotic - type drugs. From the above descriptions, drug abuse can be assumed to have a close correlation with delinquent behaviour. V. Social Factors Related to Drug Abuse As for the Korean youths, glue-sniffing is found to he related to aggressive delinquency, in such cases as run - aways, being picked up by the police, and taking money by force. Smoking cigarettes and drinking alcohol is found to be related to seeing adult videos and visiting drinking establishments. Hypnotics and marihuana were found to be representive of drugs which are related to degenerational delinquency, irrespective of social delinquency. The social factors connected with these drug abuse are as follows: 1. Individual factors Male students were more heavily involved in the usage of drug than females. Youths who do not attend church were more likely to be involved in drugs than those who attend. 2. Family factors The youths who were displeased with their mothers smoking and those who thought their parents did not love each other, or those whose parents had used drugs without prescription, were more likely to he drug users. 3. School factors Those youths who found school life boring, were unsuccessful in their studies, spend most of their time with friends, feel their teachers smoke too much, those who had a positive perception of their teachers smoking were likely to he drug users. To sum up, drug abusers depend on the influence of their parents, teachers and peers. IV. Reasons for Drug Abuse Korean students have mainly used drugs to release stress (42.8%), to stay awake (19.7%), and because of the easy accessibility of drugs( 16.6%). Other reasons are due to their ignorance of the side effects of the drugs (3.6%), natural curiosity (4.2%), and to increase strength(3.O%). From the above facts, the major reasons for drug abuse among Korean youths are to release stress and to stay awake in order to prepare exams. Furthermore, since drugs are readily available, we can conclude that drug abuse is caused by the school system(such as entrance exams) in Korea. VII. Conclusion Drug usage among Korean youths are relatively less common than those of western youths. In some cases, such as, glue-sniffing and use of stimulants, the pattern of drug abuse is found. Moreover, early drug abuse is evident, and it has a close connection with deviant behaviour, resulting in juvenile delinquency. Drug abuse cannot be attributed to any one social factor. Specifically, drug abuse depends on parents, peers, teachers and other members of the community, and also is influenced by social institutions such as the entrance exam system. Every person and organization concerned with youth must participate collectively in restraining drug abuse. Finally, it is suggested that social agencial working for youth welfare should make every effort to tackle this serious problem confronted by the Korean youths today.

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