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http://dx.doi.org/10.5142/JGR.2009.33.2.143

Optimization of Submerged Culture Conditions for the Growth Increase of Ginseng Adventitious Root Containing Germanium  

Chang, Eun-lung (Dept. of Food Science and Technology)
Oh, Roon-II (Dept. of Food Science and Technology)
Publication Information
Journal of Ginseng Research / v.33, no.2, 2009 , pp. 143-148 More about this Journal
Abstract
This study was carried out to detennine the optimal submerged culture conditions for the growth increase of ginseng adventitious roots containing germanium by means of a fractional factorial design with four factors and three levels, using the response surface methodology (RSM). The ginseng (Panax ginseng CA. Meyer) adventitious roots were induced by plant growth regulators and cultured in a liquid SH medium. The effects of various $GeO_2$ and phosphoric acid ($H_3P0_4$) concentrations in the medium, $GeO_2$ addition time and the pH of the medium on the fresh weight of the ginseng adventitious roots were investigated. The optimum pH of the medium and the phosphoric acid concentration detennined by the partial differentiation of the model equation were 4.7 and 6.0 roM, respectively. The predicted optimal $GeO_2$ concentration was 10 ppm and the $GeO_2$ addition time did not affect the growth of ginseng adventitious roots. Under these conditions, the growth of the ginseng adventitious root containing germanium was predicted to be 2.47 g.
Keywords
Ginseng; adventitious root; germanium; fresh weight; response surface methodology;
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Times Cited By KSCI : 4  (Citation Analysis)
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