• Reproductive and Developmental Biology
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• v.35 no.3
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• pp.221-225
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• 2011

This study was undertaken to evaluate the relationship between in vitro maturation and plasminogen activators (PAs) activity on porcine cumulus-oocytes complexes (COCs) exposed to oxidative stress. When COCs were cultured in maturation medium with hydrogen peroxide ($H_2O_2$), the proportion of the germinal vesicle breakdown (GVBD) and oocytes maturation were decrease with addition of $H_2O_2$, and were significantly (p<0.05) lower in medium with 0.1 mM $H_2O_2$ than control group. Also, the rate of degenerated oocytes was increased in as $H_2O_2$ concentration in eased. When COCs were cultured for 48 h, three plasminogen-dependent lytic bands were observed: tissue-type PA (tPA); urokinase-type PA (uPA); and tPA-PA inhibitor (tPA-PAI). PA activity was quantified using SDS-PAGE and zymography. When $H_2O_2$ concentration was increased, tPA and tPA-PAI activities also increased in porcine oocytes cultured for 48 h, but not uPA. In other experiment, embryos were divided into three groups and cultured in (1) control medium, (2) control medium with 1.0 mM $H_2O_2$ and (3) control medium with 1.0 mM $H_2O_2$ along with catalase in concentrations of 0.01, 0.1, and 1.0 mg/ml, respectively. $H_2O_2$ decreased the rate of GVBD and maturation in porcine COCs but catalase revealed protective activity, against oxidative stress caused by $H_2O_2$. In this experiment, tPA and tPA-PAI activities were higher in media with 1.0 mM $H_2O_2$ alone. Increasing concentration of catalase decreased tPA and tPA-PAI activities in porcine oocytes. These results indicate that the exposure of porcine follicular oocytes to ROS inhibits oocytes maturation to metaphase-II stage and increase the oocytes degeneration. Also, we speculated that increased ROS level may trigger tPA and tPA-PAI activities in porcine oocytes matured in vitro.

• Journal of Institute of Control, Robotics and Systems
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• v.7 no.10
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• pp.812-818
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• 2001

This paper proposes an optimum design method of structural and control systems, taking a 2-D truss structure as an example. The structure is supposed to be subjected to initial static loads and disturbances. For the structure, a FEM model is formed, and using modal transformation, the equation of motion is transformed into that of modal coordinates in order to reduce the D.O.F. of the FEM model. The structure is controlled by an output feedback $H^$\infty$$ controller to suppress the effect of the disturbances. The design variables of the simultaneous optimal design of control-structure systems are the cross sectional areas of truss members. The structural objective function is the structural weight. The control objective function is the $H^$\infty$$ norm, that is, the performance index of control. The second structural objective function is the energy of the response related to the initial state, which is derived from the time integration of the quadratic form of the state in the closed-loop system. In a numerical example, simulations have been carried out. Through the consideration of structural weight and $H^$\infty$$ norm, an advantage of the simultaneous optimum design of structural and control systems is shown. Moreover, while the optimized performance index of control is almost kept, we can acquire better design of structural strength.

• Korean Journal of Plant Resources
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• v.32 no.1
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• pp.53-62
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• 2019

The purpose of this study was to establish the in vitro propagation system by shoot tip culture of six Hosta species native to Korea (Hosta capitata (Koidz.) Nakai, H. clausa Nakai, H. jonesii M.G.Chung, H. minor (Baker) Nakai, H. venusta F.Maek., and H. yingeri S.B.Jones) for mass proliferation and a new cultivar development. The shoot tips of each Hosta species were cultured on MS medium containing eight combinations of 0.5, 1.0, 2.0, 4.0 mg/L BA with 0.1 mg/L NAA, 0.1, 0.5, 1.0, 2.0 mg/L TDZ with 0.1 mg/L NAA, and without any PGRs (control). They were investigated on callus, somatic embryo, crown bud, differentiation and growth of shoot and root, total fresh weight after 8 weeks of culture. In all six Hosta species, callus and somatic embryo induction rate and multiple shooting rate of the PGRs treatment group were higher than that of the control group. The highest number of differentiated shoots were obtained on medium supplemented with 2.0 ㎎/L TDZ in H. capitata (5.4), 1.0 mg/L TDZ in H. clausa and H. jonesii (3.3 and 5.8, respectively), 0.5 mg/L BA in H. minor (11.1), 1.0 mg/L BA and 0.1 mg/L TDZ in H. venusta (8.1), and 0.5 mg/L TDZ in H. yingeri (9.8). In somatic embryo formation, the PGRs treatment group of H. jonesii and H. yingeri were more effective than the control group, and the effects were relatively less in H. capitata, H. clausa Nakai, H. minor, H. venusta. Crown bud formation of four Hosta species (H.capitata, H. clausa, H. jonesiig, and H. yingeri) were also higher in the PGRs treatment group than in the control group. Crown bud formation of four Hosta species (H.capitata, H. clausa, H. jonesiig, and H. yingeri) were also higher in the PGRs treatment group than in the control group. H. clausa showed no significant effect on callus and shoot differentiation regardless of the type and concentration of cytokinin, but slightly increased in formation of crown bud in TDZ.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2008.11a
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• pp.21-22
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• 2008

• Bulletin of the Korean Chemical Society
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• v.34 no.10
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• pp.3065-3072
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• 2013

Direct synthesis of $H_2O_2$ and in situ oxidative desulfurization of model fuel over Au/Ti-HMS and Au/TS-1 catalysts has been comparatively investigated in water or methanol. Maximum amount (82%) of active $Au^0$ species for $H_2O_2$ synthesis was obtained. Au/Ti-HMS and Au/TS-1 exhibited the contrary performances in $H_2O_2$ synthesis as $CH_3OH/H_2O$ ratio of solvent changed. $H_2O_2$ decomposition and hydrogenation in water was inhibited by the introduction of methanol. Effect of $O_2/H_2$ ratio on $H_2O_2$ concentration, $H_2$ conversion and $H_2O_2$ selectivity revealed a relationship between $H_2O_2$ generation and $H_2$ consumption. The highest dibenzothiophene removal rate (83.2%) was obtained over Au/Ti-HMS in methanol at 1.5 of $O_2/H_2$ ratio and $60^{\circ}C$. But removal of thiophene over Au/TS-1 should be performed in water without heating to obtain a high removal rate (61.3%). Meanwhile, $H_2$ conversion and oxidative desulfurization selectivity of $H_2$ were presented.

• Reproductive and Developmental Biology
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• v.29 no.2
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• pp.93-99
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• 2005

Human embryonic stem cells (hESCs) derived from the inner cell mass of blastocysts have the ability to renew themselves and to differentiate into cell types of all lineage. The present study was carried out to investigate whether the Wnt signaling pathway is related to maintaining self-renewal of hESCs. Glycogen Synthase Kinase 3 (GSK-3) inhibitor, BIO ((2'Z,3'E)-6-Bromoindirubin-3'-oxime) was treated to Miz-hES1 line for activation of Wnt signaling pathway. BIO-nontreated hESCs (control) and BID-treated hESCs were cultured for 5 days in the modified feeder-free system. During the culture of hESCs, differences were observed in the colony morphology between 2 groups. Controls were spread outwards whereas BIO-nontreated hESCs were clumped in the center and the differentiated cells were spreading outwards in the edges. The results of stem cell specific marker staining indicated that control were differentiated in large part whereas BIO-treated hESCs maintain self-renewal in the center of the colony. The results of lineage marker staining suggested that outer cells of the hESC colony were differentiated to the neuronal progenitor cells in both control and BIO-treated hESC. These results indicate that Wnt signaling is related to self-renewal in hESCs. In addition, control group showed higher composition of apoptotic cells $(23.76\%)$ than the BID-treated group $(5.59\%)$. These results indicate that BIO is effective on antapoptosis of hESCs.

• Journal of Veterinary Clinics
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• v.3 no.1
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• pp.265-276
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• 1986

Pathological study was made of 173 gallbladders among 652 slaughter pigs. The size and the pH of the bile juice were made of 50 among 173 gallbladders with lesions and 50 normal gallbladders as control. The results are summerized as follows : 1. The histological lesions were shown hyperemia and congestion(83.2%), Iymphocytic infiltration(37.5%), Iympoid hyperplasia(27%), epithelial downgrowth(27%), erosion and ulcer(22%), papillary hyperplasia (20.2%), pericholecystic edema(19.5%), hemorrhage(11%), and leukemic cell infiltration(0.6%). 2. In the gallbladders with lesions mean size was 4.26${\times}$7.88cm in diameter and mean pH is 7.2. In the control means size and mean pH were 4.57${\times}$8.13cm in diameter and 7.1. The difference of size between gallbladders with lesions and these as control was not statistically significant. The pH of bile juice between two showed also no statistically significant difference.

• The Transactions of the Korean Institute of Electrical Engineers D
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• v.52 no.7
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• pp.401-409
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• 2003

This paper describes the design and the performance analysis of an $H_2$ controller for noncollocated active vibrating systems. An experiment for the active vibration control of a flexible structure is performed. The experimental model used is a cantilever beam controlled by an active damping system consisting of a laser sensor and an electromagnetic actuator. The $H_2$ controller design is based on the reduced order model and the designed system is capable of attenuating vibration without causing spillover instability. The design procedure to prevent spillover instability is described via the sensitivity analysis. The performances of the controller are verified by experimental results.

• Journal of international Conference on Electrical Machines and Systems
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• v.2 no.2
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• pp.197-201
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• 2013

Cascaded H-bridge multilevel inverter requires independent DC voltage sources to produce multi output voltage levels. When it needs to generate more levels in the output voltage wave, the number of independent DC voltage sources usually limits its extension. To solve this problem, we propose a cascaded H-bridge multilevel inverter employing a front-end flyback converter for unifying input DC voltage sources. After theoretical analysis of the proposed circuit, we verify the validity of the proposed inverter using computer-aided simulations and experiments.

• Archives of Pharmacal Research
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• v.12 no.1
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• pp.1-4
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• 1989

6-Arylamino-3,7-dihydro-3,7-dimethy-2-oxo-1H-purine and 2-arylimino-6-arylamino-3,7-dihydro-3,7-dimethyl-1H-purine were obtained in a one-pot reaction when 3,7-dihydro-3,7-dimethyl-1H-purine-3,6-dione, phosphorus pentaoxide, triethylamine hydrochloride and appropriate amine amino are heated at $170^{\circ}$. Some derivatives were tested for their antitumor activity.