• Korean Chemical Engineering Research
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• v.50 no.4
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• pp.659-665
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• 2012

IgY (Immunoglobulin Yolk) in egg yolk corresponds to IgG (Immunoglobulin G) in animal serum and plays an important role as immunological proteins in intestines. Carrageenan and Arabic gum were used as pretreatment agents to purify IgY from fresh egg yolk. DEAE (Diethylaminoethyl) Sepharose column in FPLC (Fast Protein Liquid chromatography) was an ion exchange tool to remove contaminants as well as to elute IgY from the column. GF HPLC (Gel Filtration High Performance Liquid Chromatography) enables to measure the molecular weights of IgY and to identify the purified IgY by comparing the molecular weight of standard IgY with the purified one. IgY is a heterogeneous group of different molecular weight and ionic properties, which was investigated with various IE HPLC (Ion Exchange High Performance Liquid Chromatography) columns such as AX, CX and SCX. Three peaks of IgY were separated in the AX column under the conditions of 0.5 M NaCl and pH=8. The SCX column also gave the three peaks of IgY at 0.5 M NaCl and pH=5.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.30 no.3C
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• pp.176-184
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• 2005

In this paper, we implemented an Elliptic Curve Cryptography(ECC) processor for Digital Transmission Contents Protection (DTCP), which is a standard for protecting various digital contents in the network. Unlikely to other applications, DTCP uses ECC algorithm which is defined over GF(p), where p is a 160-bit prime integer. The core arithmetic operation of ECC is a scalar multiplication, and it involves large amount of very long integer modular multiplications and additions. In this paper, the modular multiplier was designed using the well-known Montgomery algorithm which was implemented with CSA(Carry-save Adder) and 4-level CLA(Carry-lookahead Adder). Our new ECC processor has been synthesized using Samsung 0.18 m CMOS standard cell library, and the maximum operation frequency was estimated 98 MHz, with the size about 65,000 gates. The resulting performance was 29.6 kbps, that is, it took 5.4 msec to process a 160-bit data frame. We assure that this performance is enough to be used for digital signature, encryption and decryption, and key exchanges in real time environments.

• Journal of the Korean Institute of Telematics and Electronics
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• v.27 no.12
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• pp.1878-1888
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• 1990

In this paper, the input-output interconnection method of the multi-valued signal processing circuit using perfect Shuffle technique and Kronecker product is discussed. Using this method, the design method of circuit of the multi-valued Reed-Muller expansions(MRME) to be used the multi-valued signal processing on finite field GF(p**m) is presented. The proposed input-output interconnection method is shown that the matrix transform is efficient and that the module structure is easy. The circuit design of MRME on FG(p**m) is realized following as` 1) contructing the baisc gates on GF(3) by CMOS T gate, 2) designing the basic cells to be implemented the transform and inverse transform matrix of MRME using these basic gates, 3) interconnecting these cells by the input-output interconnecting method of the multivalued signal processing circuits. Also, the circuit design of the multi-valued signal processing function on GF(3\ulcorner similar to Winograd algorithm of 3x3 array of DFT (discrete fourier transform) is realized by interconnection of Perfect Shuffle technique and Kronecker product. The presented multi-valued signal processing circuits that are simple and regular for wire routing and posses the properties of concurrency and modularity are suitable for VLSI.

• Journal of the Korea Institute of Information Security & Cryptology
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• v.29 no.3
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• pp.511-514
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• 2019

Elliptic Curves Cryptosystem(ECC) provides the same level of security with relatively small key sizes, as compared to the traditional cryptosystems. The performance of ECC over GF(2m) and GF(p) depends on the efficiency of finite field arithmetic, especially the modular multiplication which is based on the reduction algorithm. In this paper, we propose a new modular reduction algorithm which provides high-speed ECC over NIST prime P-256. Detailed experimental results show that the proposed algorithm is about 25% faster than the previous methods.

• Journal of the Korea Institute of Information Security & Cryptology
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• v.7 no.4
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• pp.127-132
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• 1997

본 논문은 유한체 GF(p)에서 r=2p+1이 2-prime이고, p에 대한 위수(order) m을 가질때, q=r$^{e}$ (e$2)를 연결 정수로 갖는 FCSR의 생성된 출력수열에 대한 선형 복잡도의 하한을 구한다.

• The KIPS Transactions:PartA
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• v.11A no.2
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• pp.115-122
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• 2004

In this paper, the multiple-valued adders and multipliers are implemented by current-mode CMOS. First, we implement the 3-valued T-gate and the 4-valued T-gate using current-mode CMOS which have an effective availability of integrated circuit design. Second we implement the circuits to be realized 2-variable 3-valued addition table and multiplication table over finite fields $GF(3^2)$, and 2-variable 4-valued addition table and multiplication table over finite fields $GF(4^2)$ with the multiple-valued T-gates. Finally, these operation circuits are simulated under $1.5\mutextrm{m}$ CMOS standard technology, $15\mutextrm{A}$ unit current, and 3.3V VDD voltage Spice. The simulation results have shown the satisfying current characteristics. The 3-valued adder and multiplier, and the 4-valued adder and multiplier implemented by current-mode CMOS is simple and regular for wire routing and possesses the property of modularity with cell array. Also, since it is expansible for the addition and multiplication of two polynomials in the finite field with very large m, it is suitable for VLSI implementation.

• Journal of Korea Society of Industrial Information Systems
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• v.12 no.1
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• pp.28-38
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• 2007

In this paper, we implement Microsoft COM software modules for elliptic curve cryptographic applications and analyze its performance. The implemented COM software modules support all elliptic curve key exchange protocols and elliptic curve digital signature algorithm in IEEE 1363 finite fields GF(p) and GF(2m). Since the implemented software modules intend to focus on a component-based software development method, and thus it have a higher productivity and take systematic characteristics to be open outward and to be standardized. Accordingly, it enable a software to be developed easier and faster rather than a method using C library. In addition it support the Microsoft COM interface, we can easily implement secure software applications based on elliptic curve cryptographic algorithms.

• Restorative Dentistry and Endodontics
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• v.31 no.3
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• pp.153-160
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• 2006

We investigated the secretion of Interleukin-8 (IL-8) from ginviva and periodontal ligament stimulated with Substance P (SP) and Calcitonin Gene-related Peptide (CGRP). Gingiva (GF), periodontal ligament (PDLF) and pu)p (PF) tissues were collected from extracted intact 3rd molars. Cultured cells were stimulated with different concentrations of SP for 4 hrs, and stimulated with SP, CGRP and Tumor Necrosis Factor-$\alpha$ (TNF-$\alpha$) for 8 hrs. Then RNase Protection Assay was carried out. ELISA was performed using supernatants of stimulated cells for quantitative analysis of IL-8. Results were assessed using student t-test with significance of P<0.05. According to this study, the results were as follows: 1. IL-8 mRNA was detected in all type of cells studied (PF, GF and PDLF) 2. IL-8 mRNA expression was not increased after stimulating 4 hrs with SP ($10^{-5}M$) and SP ($10^{-8}M$) compared with Mock stimulation in all type of cells studied. 3. IL-8 mRNA expression was not increased after stimulating 8 hrs with SP ($10^{-4}M$) and CGRP ($10^{-6}M$) compared with Mock stimulation in all type or cells studied. 4. TNF-$\alpha$ (2 ng/ml) increased the expression of IL-8 mRNA in all kind of cells studied. 5. The secretion of IL-8 from GF was increased 8 hrs after the stimulation with CGRP ($10^{-6}M$)(p<0.05). 6. The secretion of IL-8 from PDLF was. increased 8 hrs after the stimulation with SP ($10^{-4}M$)(p<0.05). Calcitonin Gene-related Peptide (CGRP) increased Interleukin-8 (IL-8) which plays an important role in chemotaxis of neutrophil in Calcitonin Gene-related Peptide (CGRP) gingival tissue , whereas Substance P increased the secretion of IL-8 from periodontal ligament.

• Journal of Ginseng Research
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• v.47 no.3
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• pp.420-428
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• 2023

Background: Ginsenoside F2 (GF2), a minor component of Panax ginseng, has been reported to possess a wide variety of pharmacological activities. However, its effects on glucose metabolism have not yet been reported. Here, we investigated the underlying signaling pathways involved in its effects on hepatic glucose. Methods: HepG2 cells were used to establish insulin-resistant (IR) model and treated with GF2. Cell viability and glucose uptake-related genes were also examined by real-time PCR and immunoblots. Results: Cell viability assays showed that GF2 up to 50 μM did not affect normal and IR-HepG2 cell viability. GF2 reduced oxidative stress by inhibiting phosphorylation of the mitogen-activated protein kinases (MAPK) signaling components such as c-Jun N-terminal kinase (JNK), extracellular signal-regulated kinase 1/2 (ERK1/2), and p38 MAPK, and reducing the nuclear translocation of NF-κB. Furthermore, GF2 activated PI3K/AKT signaling, upregulated the levels of glucose transporter 2 (GLUT-2) and GLUT-4 in IR-HepG2 cells, and promoted glucose absorption. At the same time, GF2 reduced phosphoenolpyruvate carboxykinase and glucose-6-phosphatase expression as well as inhibiting gluconeogenesis. Conclusion: Overall, GF2 improved glucose metabolism disorders by reducing cellular oxidative stress in IR-HepG2 cells via MAPK signaling, participating in the PI3K/AKT/GSK-3β signaling pathway, promoting glycogen synthesis, and inhibiting gluconeogenesis.

• Restorative Dentistry and Endodontics
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• v.36 no.1
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• pp.26-36
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• 2011

Objectives: In the present study, three kinds of tissues cells (pulp, gingiva, and periodontal ligament) were investigated if those cells express MMP and TIMP when they were stimulated with neuropeptides (substance P, CGRP) or proinflammatory cytokine, TNF-$\alpha$. Materials and Methods: The cells cultured from human dental pulp (PF), gingiva (GF) and periodontal ligament were (PDLF) stimulated with Mock, SP, TNF-$\alpha$, and CGRP for 24 hrs and 48 hrs. for an RNase protection assay and Enzyme Linked Immunosorbent Assay. Cells (PF, GF and PDLF) seeded in 100 mm culture dish were stimulated with SP ($10^{-5}$, $10^{-8}\;M$) or only with medium (Mock stimulation) for 4hrs and for 24 hrs for RNase Protection Assay, and they were stimulated with CGRP ($10^{-5}\;M$) and TNF-$\alpha$(2 ng/mL) for 24 hrs and with various concentraion of TNF-$\alpha$(2, 10, and 100 ng/mL) for Rnase Protection Assay with a human MMP-1 probe set including MMP 1, 2, 8, 7, 8, 9, 12, and TIMP 2, 3. In addition, cells (PF, GF and PDLF) were stimulated with Mock and various concentraion of TNF-$\alpha$(2, 10, and 100 ng/mL) for 24 hrs and with TNF-$\alpha$(10 ng/mL) for 48 hrs, and the supernatents from the cells were collected for Enzyme Linked Immunosorbent Assay (ELISA) for MMP-1 and MMP-13. Results: The expression of MMPs in PF, GF, PDLF after stimulation with SP and CGRP were not changed compared with Mock stimulation for 4 hrs and 24 hrs. The expression of MMP-1, -12, -13 24 hrs after stimulation with TNF-$\alpha$ were upregulated, however the expression of TIMP-3 in PF, GF, PDLF after stimulation with TNF-$\alpha$ were downregulated. TNF-$\alpha$(2 ng/mL, 10 ng/mL, 100 ng/mL) increased MMP-1 and MMP-12 expression in PF dose dependently for 24 hrs. Conclusions: TNF-$\alpha$ in the area of inflammation may play an important role in regulating the remodeling of dentin, cementum, and alveolar bone.