• Title/Summary/Keyword: $E_iC_{50}$

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Synthesis of Hydrazone Derivatives of 4-(2-Chloroethyl) semicarbazide : A New Class of Cytotoxic Agents (4-(2-Chloroethyl) semicarbazide의 히드라존 유도체 합성:새로운 종류의 세포독성요법제)

  • El-Sabbagh, O.I.;El-Sadek, M.E.;Aboukull, M.E.;Shallal, H.M.
    • Journal of the Korean Chemical Society
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    • v.53 no.1
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    • pp.34-41
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    • 2009
  • A new series of hydrazone derivatives were synthesized from 4-(2-chloroethyl)semicarbazide and their antiproliferative activity against human brain (U251) and liver (Hepg2) carcinoma cell lines were evaluated. The hydrazone compounds are benzaldehyde (2a-2g), acetophenone (3a-3f), and 3-formylindole derivatives (4a-4d). Among the acetophenone derivatives, 3e (p-methoxy substituted) and 3f (p-nitro substituted) showed the highest cytotoxic activity against Hepg2 cell line (I$C_{50}$ = 6 and 8 $\mu$g/ml, respectively). Among the 3-formylindole derivatives, 4a (hydrazone of 3-formylindole itself) showed a pronounced cytotoxic activity against both U251 (I$C_{50}$ = 21 $\mu$g/ml) and Hepg2 (I$C_{50}$ = 7 $\mu$g/ml).

Acute and Chronic Toxicity of heavy Metals to Daphnia magna (물벼룩(Daphnia magna)에 대한 중금속의 급성 및 만성 독성)

  • Jeong, Jae-Won;Cha, Mi-Seon;Jo, Sun-Ja;Lee, Sang-Jun
    • Journal of Environmental Science International
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    • v.10 no.4
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    • pp.293-298
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    • 2001
  • The toxicity values heavy metals were evaluated by immobilization and chronic reproduction impairment tests, using Daphnia magna. Acute tests were evaluated by the inhibition of their mobilization after 24hrs without food addition. The tests of reproductive impairment were investigated for 21 days by food addition and exchange or water. The effect of each concentration was assessed by Probit analysis and t-test. The results obtained from this study were as follows : 1) The change of pH and DO was not significant in the acute tests, while, in the reproductive tests, pH was increased by 0.3~1.4 and DO also increased. 2) The $E_iC_{50}$ values of immobilization to Daphnia magna in artificial fresh water were $0.030mg/{\ell}(Cu),\;0.054mg/{\ell}(Cd),\;0.12mg/{\ell};(Cr),\;0.74mg/{\ell}(Pb),\;3.4mg/ {\ell}(As)$ and the $NOE_iC$ values were $0.010mg/{\ell}(Cu),\;0.018mg/{\ell}(Cd),\;0.010mg/{\ell}(Cr),\;0.10mg/{\ell}(Pb),\;and\;$1.8mg/{\ell}(As)$. 3) The $E_rC_{50}$ values of reproductive impairment to Daphnia magna were $13.8\mu\textrm{g}/{\ell}(Cu),\;2.9\mu\textrm{g}/{\ell}(Cd),\;15.5\mu\textrm{g}/{\ell}(Cr),\;61.7\mu\textrm{g}/{\ell}(Pb),\;759\mu\textrm{g}/{\ell}(As)$, and $NOE_rC$ values were $0.95\mu\textrm{g}/{\ell}(Cu),\;$0.54\mu\textrm{g}/{\ell}(Cd),\;1.2\mu\textrm{g}/{\ell}(Cd),\;$7.4\mu\textrm{g}/{\ell}(Pb),\;110mu\textrm{g}/{\ell}(As)$. The results of tests using OECD artificial culture water were more sensitive than natural water for culturing. The presented data show that an artificial culture water is suitable in the experiment of bioassay for assessing the toxicity of marterials.

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Deposition of Poly-$Si_{1-x}Ge_x$ Thin Film by RTCVD (RTCVD에 의한 다결정 $Si_{1-x}Ge_x$ 박막 증착)

  • Kim, Jae-Jung;Lee, Seung-Ho;So, Myeong-Gi
    • Korean Journal of Materials Research
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    • v.5 no.6
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    • pp.690-698
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    • 1995
  • The Poly-S $i_{1-x}$G $e_{x}$ thin films were deposited on oxidized Si wafer by RTCVD(rapid thermal chemical vapor deposition) using Si $H_4$and Ge $H_4$, at 450 ~5$50^{\circ}C$. The variation of Ge mole fraction and the deposition rate of S $i_{1-x}$G $e_{x}$ thin film were studied as a function of the deposition temperature and the Ge $H_4$/Si $H_4$input ratio, and the crystal phase and the surface roughness were studied by XRD and AFM(atomic force microscopy), respectively. The experimental results showed that the activation energy for the deposition of poly-S $i_{1-x}$G $e_{x}$ was about 32~37Kca /mol and the deposition rate of S $i_{1-x}$G $e_{x}$ thin films was increased with increasing the deposition temperature and the input ratio. From the analysis of composition, it was known that the Ge mole fraction within the poly-S $i_{1-x}$G $e_{x}$ thin film was decreased with decreasing the input ratio and increasing the deposition temperature. As-deposited S $i_{1-x}$G $e_{x}$ thin films were polycrystalline over the entire experimental range. But those were amorphous at the deposition temperature of 450, 475$^{\circ}C$ and the input ratio of 0.05. By adding the Ge $H_4$, poly-S $i_{1-x}$G $e_{x}$ thin film were deposited at relatively lower deposition temperatures($\leq$ 5$50^{\circ}C$) than those of conventional poly-Si(>$600^{\circ}C$). From surface roughness measurement of poly-S $i_{1-x}$G $e_{x}$ it was found that the surface roughness( $R_{i}$ ) increased with increasing the deposition temperature and input ratio.and input ratio.

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Modulation of arachidonic acid metabolism and inflammatory process in macrophages by different solvent fractions of Glasswort (Salicornia herbacea L.) extract (큰포식세포에서 퉁퉁마디 추출물의 아라키돈산 대사효소조절 및 항염증 활성)

  • Kang, Smee;Choi, YooMi;Hong, Jungil
    • Korean Journal of Food Science and Technology
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    • v.50 no.6
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    • pp.671-679
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    • 2018
  • Glasswort has attracted an attention because of its interesting physiological actions. In this study, the effects of glasswort on inflammatory events including nitric oxide (NO) synthesis and arachidonic acid metabolism in cultured RAW264.7 macrophages were investigated. A series of solvent fractions, including fractions of hexane (Fr.H), ethyl ether (Fr.E), ethyl acetate, butanol, and water, were prepared from a 70% methanol extract of glasswort. Among the fractions, Fr.E showed the strongest inhibition of NO synthesis and inducible NO synthase (iNOS) expression in lipopolysaccharide (LPS)-stimulated macrophages. At a concentration of $80{\mu}g/mL$, Fr.E decreased the NO and iNOS levels by 73 and 77%, respectively, after 24 h. Fr.E showed the most potent inhibitory effects on the expressions of cytosolic phospholipase $A_2$ and cyclooxygenase-2 with $IC_{50}$ values of 33.4 and $27.9{\mu}g/mL$, respectively. Fr.H and Fr.E also significantly inhibited 5-lipoxygenase expression in LPS-stimulated macrophages. These results suggest that the hydrophobic fractions of glasswort possess anti-inflammatory activities through modulating the arachidonic acid metabolism and NO synthesis.

Flavor Charateristics in Kiwifruit Pulp(Actinidia chinensis Planch) (양다래 펄프의 향기특성)

  • Lee, Kyoung-Hae
    • Korean Journal of Food Science and Technology
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    • v.31 no.2
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    • pp.342-347
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    • 1999
  • Kiwifruit pulp was separated into insoluble pulp and serum by centrifugation at 14,000 rpm for 10 min. The serum portion was concentrated at $30{\sim}50mmHg\;and\;50{\sim}55^{\circ}C$ with aroma recovery. Vapor generated at the early stage of vacuum evaporation was condensed and taken as aroma fractions: $E-I\;(0{\sim}10%),\;E-II,\;(10{\sim}20%)$. The volatile flavor compounds in kiwifruit pulp were collected by dynamic headspace technique and analyzed by GC and GC/MSD. The yield of serum separated from kiwifruit pulp was 70.1% and insoluble pulp fraction contained aroma compounds more than that of the serum. Twenty-six aroma compounds, including (E)-2-hexanal and ethyl butanoate were detected. The efficiency of the aroma recovery was reduced as the recovery time was extended, as indicated by the less peak numbers and kiwifruit areas of aroma fractions.

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Is that possible to simulate daily runoff with one parameter? (하나 매개변수로 유출 모의 가능한가?)

  • Noh, Jaekyoung;An, Hyunuk;Lee, Jaenam
    • Proceedings of the Korea Water Resources Association Conference
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    • 2017.05a
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    • pp.29-29
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    • 2017
  • 유역의 물수지를 강수, 증발산, 토양수분저류, 유출 등 성분으로 구성하고, 토양수분저류 상태에 따라 증발산과 유출이 변화하는 식을 기본식으로 구성하였으며, 물수지를 개선하는 매개변수를 변수화하는 개념을 도입하여 다음 식의 모형을 개발하였다. 여기서, ETa는 실제증발산량, ETo는 잠재증발산량, Q는 유출량, S는 토양수분저류량이고, C1은 증발산, C2, ${\alpha}$는 유출반응, C3, C4는 매개변수 ${\alpha}$를 변수화시키는 데 관련한 매개변수이다. $$ETa(i)=(1-e^{-c1{\times}s(i)}){\times}ETo_{(i)}$$ $$Q_{(i)}=S_{(i)}{\times}(1-e^{-c2{\times}s(i)})^{[(c3+e^{-c4{\times}s(i)}){\times}a]}$$ 모형의 검증을 위해 Monte Calro 기법으로 최적 매개변수를 결정한 결과 수많은 매개변수 조합이 최적영역에 분포되는 것을 확인하였으며, 이를 바탕으로 매개변수 하나만 남겨 놓고 나머지 매개변수는 상수화시켜도 모의결과가 똑같다는 결과를 관찰하였으며, 이를 토대로 하나 매개변수만으로 일 유출 모의가 가능하다고 결론을 내렸다. 하나의 매개변수는 ${\alpha}$를 우선 추천하고, C1도 유역의 토지이용에 따라 증발산이 변화하기 때문에 의미있다고 판단하고 있다. 하나의 매개변수를 결정하는 방법은 유출 자료가 있으면 유출량으로, 없으면 유출률을 맞추는 방법이며, 일반화하기 쉽고 실용성이 매우 높은 것으로 평가된다. 유역면적 $209km^2$인 보령댐의 2007년부터 2009년까지 Monte Calro 기법으로 매개변수를 결정한 결과 C1=0.0196, C2=0.0023, C3=0.3230, C4=0.0051, ${\alpha}=2.3304$ 이었으며, 이 때 연평균 강우량 1221.2mm, 유출량 651.2mm, 유출률 53.3%이었으며, $R^2=0.833$, RMSE=2.073, NSE=0.831이었고, 관측 유출량 610.8mm, 유출률 50.0%였다. 매개변수 C1, C2, C3, C4를 고정시키고 유출률 50%에 이를 때 ${\alpha}$는 2.6946이었으며, 이 때 $R^2=0.831$, RMSE=2.102, NSE=0.826이었고, 매개변수 C2, C3, C4, ${\alpha}$를 고정시키고 유출률 50%에 이를 때 C1은 0.0255이었으며, 이 때 $R^2=0.833$, RMSE=2.083, NSE=0.829이었다. 한편 똑같은 자료로 탱크모형은 $R^2=0.79$, RMSE=2.43, NSE=0.77이었고, SWAT 모형은 $R^2=0.56$, RMSE=3.97, NSE=0.40으로 나타난 것과 비교할 때, 개발된 모형의 성능이 우수한 것이라 결론내릴 수 있었다.

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Inhibitors of Melanogenesis from Atractylodes rhizomes (백출의 멜라닌 생성 억제 물질)

  • Jung, Min-Hwan;Moon, Chul-Soon;Lim, Young-Hee;Kang, Sang-Jin;Cho, Wan-Goo;Kim, Cheong-Taek
    • Korean Journal of Pharmacognosy
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    • v.36 no.1 s.140
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    • pp.60-63
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    • 2005
  • One sesquiterpenoid was isolated from the methanol extract of Atractylodes rhizomes which has been used as traditional medicine for diuresis and the compound was established as selina-4(14),7(11)-dien-8-one by the spectroscopic methods. The compound$(IC_{50}<10\;ppm)$ has inhibitory effects on melanogenesis in B16 mouse melanoma.

Improved dentin disinfection by combining different-geometry rotary nickel-titanium files in preparing root canals

  • Bedier, Marwa M.;Hashem, Ahmed Abdel Rahman;Hassan, Yosra M.
    • Restorative Dentistry and Endodontics
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    • v.43 no.4
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    • pp.46.1-46.10
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    • 2018
  • Objectives: This study was to evaluate the antibacterial effect of different instrumentation and irrigation techniques using confocal laser scanning microscopy (CLSM) after root canal inoculation with Enterococcus faecalis (E. faecalis). Materials and Methods: Mesiobuccal and mesiolingual canals of extracted mandibular molars were apically enlarged up to a size 25 hand K-file, then autoclaved and inoculated with E. faecalis. The samples were randomly divided into 4 main groups according to the system of instrumentation and irrigation: an XP-endo Shaper (XPS) combined with conventional irrigation (XPS/C) or an XP-endo Finisher (XPF) (XPS/XPF), and iRaCe combined with conventional irrigation (iRaCe/C) or combined with an XPF (iRaCe/XPF). A middle-third sample was taken from each group, and then the bacterial reduction was evaluated using CLSM at a depth of $50{\mu}m$ inside the dentinal tubules. The ratio of red fluorescence (dead cells) to green-and-red fluorescence (live and dead cells) represented the percentage of bacterial reduction. The data were then statistically analyzed using the Kruskal-Wallis test for comparisons across the groups and the Dunn test was used for pairwise comparisons. Results: The instrumentation and irrigation techniques had a significant effect on bacterial reduction (p < 0.05). The iRaCe/XPF group showed the strongest effect, followed by the XPS/XPF and XPS/C group, while the iRaCe/C group had the weakest effect. Conclusions: Combining iRaCe with XPF improved its bacterial reduction effect, while combining XPS with XPF did not yield a significant improvement in its ability to reduce bacteria at a depth of $50{\mu}m$ in the dentinal tubules.

Evaluation and Identification of Promising Bivoltine Breeds in the Silkworm Bombyx mori L.

  • Begum, Azeezur Rehman Naseema;Basavaraja, Hadikere Kallappa;Joge, Punjab Govindrai;Palit, Aditya Kumar
    • International Journal of Industrial Entomology and Biomaterials
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    • v.16 no.1
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    • pp.15-20
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    • 2008
  • Under the all India programme of evaluation of mulberry and silkworm genotypes, twelve bivoltine silkworm breeds obtained from Central Silkworm Germplasm Resource Centre, Hosur (CSGRC) were evaluated at the bivoltine silkworm breeding laboratory, Central Sericultural Research & Training Institute, Mysore (CSR&TI). These breeds were tested during September-October 2003, August-September 2004 and February-March 2005. The average temperature and humidity during September-October 2003 was $26.5^{\circ}C$ and 72.6% RH, while during August-September 2004, it was $26.5^{\circ}C$ and 75.2% RH and during February-March 2005 it was $24^{\circ}C$ and 48% RH respectively. The performance of the breeds in respect of 21 traits was studied and statistically analyzed using analysis of variance (Singh and Choudhary, 1985). Silkworm breeds were short-listed using multiple trait evaluation index method as suggested by Mano et at., (1993). Evaluation Index values were calculated for all the 11 traits of economic importance and six breeds were short-listed based on average index value 50 and above 50. Two breed viz., BV 183 (SMGS-1) have recorded average E.I. >50 in 10 traits (except in neatness) and ranked first and the breed BV 262 (SMGS9) with E.I. value >50 in nine traits except in cocoon weight and neatness ranked second, in the order of merit. These two breeds may be selected as resource material for evolving region specific silkworm breeds.

Construction of Complementary DNA Library and cDNA Cloning for Cy Strain of Odontoglossum Ringspot Virus Genomic RNA (오돈토글로썸 윤문 바이러스 Cy계통 게놈 RNA의 cDNA 구축 및 유전자 크로닝)

  • 류기현;박원목
    • Korean Journal Plant Pathology
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    • v.10 no.3
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    • pp.228-234
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    • 1994
  • Genomic RNA was extracted from Cy strain of odontoglossum ringspot tobamovirus (ORSV-Cy) isolated from infected leaves of tobacco cv. Samsun. Size of the genomic RNA was about 6.6 kb in length. The genomic RNA was fractionated using Sephadex G-50 column chromatography into 2 fractions. They were polyadenylated at their 3'-end using E. coli poly(A) polymerase. Polyadenylated viral RNA was recovered by oligo (dT) primer adapter containing NotI restriction site and Moloney murine leukemia virus SuperScript reverse transcriptase (RNase H-). Second-strand cDNA was synthesized by using E. coli DNA ligase, E. coli DNA polymerase I and E. coli RNase H. Recombinant plasmids containing cDNAs for ORSV-Cy RNA ranged from about 800 bp to 3,000 bp. Among the selected 238 recombinants, pORCY-124 clone was the largest one covering 3'-terminal half of the viral RNA. This clone contained two restriction sites for EcoRI and XbaI and one site for AccI, AvaI, BglII, BstXI, HindIII, PstI, and TthIII 1. respectively. The clone contained partial viral replicase, a full-length movement protein and a complete coat protein genes followed by a 3' untranslated region of 414 nucleotides based on restriction mapping and nucleotide sequencing analyses. Clones pORCY-028, -068, -072, -187 and -224 were overlapped with the pORCY-124. Clones pORCY-014 and -095 covered 5' half upstream from the middle region of the viral RNA, which was estimated based on restriction mapping and partial sequence analysis. Constructed cDNA library covered more than 90% of the viral genome.

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