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http://dx.doi.org/10.9721/KJFST.2018.50.6.671

Modulation of arachidonic acid metabolism and inflammatory process in macrophages by different solvent fractions of Glasswort (Salicornia herbacea L.) extract  

Kang, Smee (Division of Applied Food System, College of Natural Science, Seoul Women's University)
Choi, YooMi (Division of Applied Food System, College of Natural Science, Seoul Women's University)
Hong, Jungil (Division of Applied Food System, College of Natural Science, Seoul Women's University)
Publication Information
Korean Journal of Food Science and Technology / v.50, no.6, 2018 , pp. 671-679 More about this Journal
Abstract
Glasswort has attracted an attention because of its interesting physiological actions. In this study, the effects of glasswort on inflammatory events including nitric oxide (NO) synthesis and arachidonic acid metabolism in cultured RAW264.7 macrophages were investigated. A series of solvent fractions, including fractions of hexane (Fr.H), ethyl ether (Fr.E), ethyl acetate, butanol, and water, were prepared from a 70% methanol extract of glasswort. Among the fractions, Fr.E showed the strongest inhibition of NO synthesis and inducible NO synthase (iNOS) expression in lipopolysaccharide (LPS)-stimulated macrophages. At a concentration of $80{\mu}g/mL$, Fr.E decreased the NO and iNOS levels by 73 and 77%, respectively, after 24 h. Fr.E showed the most potent inhibitory effects on the expressions of cytosolic phospholipase $A_2$ and cyclooxygenase-2 with $IC_{50}$ values of 33.4 and $27.9{\mu}g/mL$, respectively. Fr.H and Fr.E also significantly inhibited 5-lipoxygenase expression in LPS-stimulated macrophages. These results suggest that the hydrophobic fractions of glasswort possess anti-inflammatory activities through modulating the arachidonic acid metabolism and NO synthesis.
Keywords
glasswort; macrophage; cyclooxygenase; lipoxygenase; phospholipase; nitric oxide;
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