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검색결과 586건 처리시간 0.034초

Morphological, Molecular, and Biochemical Characterization of Monounsaturated Fatty Acids-Rich Chlamydomonas sp. KIOST-1 Isolated from Korea

  • Jeon, Seon-Mi;Kim, Ji Hyung;Kim, Taeho;Park, Areumi;Ko, Ah-Ra;Ju, Se-Jong;Heo, Soo-Jin;Oh, Chulhong;Affan, Md. Abu;Shim, Won-Bo;Kang, Do-Hyung
    • Journal of Microbiology and Biotechnology
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    • 제25권5호
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    • pp.723-731
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    • 2015
  • Microalgae hold promise as producers of sustainable biomass for the production of biofuels and other biomaterials. However, the selection of strains with efficient and robust production of desirable resources remains challenging. In this study, we isolated a green microalga from Korea and analyzed its morphological, molecular, and biochemical characteristics. Microscopic and phylogenetic analyses demonstrated that the isolate could be classified into the genus Chlamydomonas, and we designated the isolate Chlamydomonas sp. KIOST -1. Compositions of protein, lipid, and carbohydrate in the microalgal cells were estimated to be 58.8 ± 0.2%, 22.7 ± 1.2%, and 18.5 ± 1.0%, respectively. Similar to other microalgae belonging to Chlorophyceae, the dominant amino acid and monosaccharide in Chlamydomonas sp. KIOST-1 were glutamic acid and glucose. On the other hand, the proportions of saturated fatty acids, monounsaturated fatty acids, and polyunsaturated fatty acids clearly differed from other species in the genus Chlamydomonas, and monounsaturated fatty acids accounted for a large portion (41.3%) of the total fatty acids in the isolate. Based on these results, Chlamydomonas sp. KIOST-1 has advantageous characteristics for biomass production.

반복자연유산에서 Methylenetetrahydrofolate Reductase 돌연변이에 대한 분석 (The Analysis of Methylenetetrahydrofolate Reductase Mutation in Recurrent Spontaneous Abortion)

  • 남윤성;차광렬;김남근;김선희;임진우;강금덕;강명서;김세현;오도연
    • Clinical and Experimental Reproductive Medicine
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    • 제28권3호
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    • pp.247-253
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    • 2001
  • Objective: To analyze the methylenetetrahydrofolate reductase (MTHFR) mutation in patients with recurrent spontaneous abortion. Material and Method: The blood samples of patients with recurrent spontaneous abortion were tested by PCR-RFLP method. Results: Of 51 cases of study group, 14 (27.5%) were normal, 25 (49.0%) were heterozygosity, and 12 (23.5%) were homozygosity. Of 58 cases of control group, 20 (34.5%) were normal, 30 (51.7%) were heterozygosity, and 8 (13.8%) were homozygosity. But the difference between two groups was not significant (p=0.190). Conclusion: Hyperhomocysteinemia due to MTHFR mutation is a cause of recurrent spontaneous abortion. Therefore, the study for MTHFR mutation should be included in the workup of recurrent spontaneous abortion.

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규소 기판 접합에 있어서 FT-IR을 이용한 수산화기의 영향에 관한 해석 (ANALYSIS OF THE EFFECT OF HYDROXYL GROUPS IN SILICON DIRECT BONDING USING FT-IR)

  • 박세광;권기진
    • 센서학회지
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    • 제3권2호
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    • pp.74-80
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    • 1994
  • Silicon direct bonding 기술은 잔류 응력이 없고, 안정한 특성을 가진 센서의 제작과 silicon-on-insulator 소자의 제조에 널리 이용되고 있다. SDB의 공정 절차는 크게 실리콘 웨이퍼의 수산화 공정 과정과 wet oxidation fumace에서 고온의 열처리 공정 과정을 거치게 된다. 수산화 공정을 행한 후, Fourier transformation-infrared spectroscopy를 사용하여 실리콘 웨이퍼 표면을 분석하여 보면, 실리콘 웨이퍼의 표면에서는 수산화기가 생성됨을 알 수 있다. 실험 결과, $H_{2}O_{2}\;:\; H_{2}SO_{4}$ 용액을 사용한 친수성 용액 처리의 경우에 있어서는 수산화기가 3474 $cm^{-1}$ 주위의 넓은 영역에서 관찰되었다. 그러나, diluted HF 용액의 경우에 있어서는 수산화기가 관찰되지 않았다. 접합된 실리콘웨이퍼를 tetramethylammonium hydroxide 식각 용액을 사용하여 식각 공정을 수행하였다. 식각 공정은 자동 식각 중지가 수행되었으며, 식각된 표면은 평탄하고 균일하였다. 그러므로, 이러한 SDB 기술은 우수한 특성을 가진 압력, 유속, 가속도 센서 등과 같은 센서의 제작 및 센서 응용 분야에 이용될 수 있을 것이다.

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고온 및 고온고습 환경 내에서 ZnO:Al 투명전극의 열화가 CIGS 박막형 태양전지의 성능 저하에 미치는 영향 (Effect of Degraded Al-doped ZnO Thin Films on Performance Deterioration of CIGS Solar Cell)

  • 김도완;이동원;이희수;김승태;박지홍;김용남
    • 한국세라믹학회지
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    • 제48권4호
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    • pp.328-333
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    • 2011
  • The influence of Al-doped ZnO (AZO) thin films degraded under high temperature and damp heat on the performance deterioration of Cu(In,Ga)$Se_2$ (CIGS) solar cells was investigated. CIGS solar cells with AZO/CdS/CIGS/Mo structure were prepared on glass substrate and exposed to high temperature ($85^{\circ}C$) and damp heat ($85^{\circ}C$/85% RH) for 1000 h. As-prepared CIGS solar cells had 64.91% in fill factor (FF) and 12.04% in conversion efficiency. After exposed to high temperature, CIGS solar cell had 59.14% in FF and 9.78% in efficiency, while after exposed to damp heat, it had 54.00% in FF and 8.78% in efficiency. AZO thin films in the deteriorated CIGS solar cells showed increases in resistivity up to 3.1 times and 4.4 times compared to their initial resistivity after 1000 h of high temperature and damp heat exposure, respectively. These results can be explained by the decreases in carrier concentration and mobility due to diffusion or adsorption of oxygen and moisture in AZO thin films. It can be inferred that decreases in FF and conversion efficiency were caused by an increase in series resistance, which resulted from an increase in resistivity of AZO thin films degraded under high temperature and damp heat.

Clinical Efficacy of Radiation-Sterilized Allografts for Sellar Reconstruction after Transsphenoidal Surgery

  • Kim, Se-Jin;Jeon, Chi-Man;Kong, Doo-Sik;Park, Kwan;Kim, Jong-Hyun
    • Journal of Korean Neurosurgical Society
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    • 제50권6호
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    • pp.503-506
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    • 2011
  • Objective : The aim of this study was to assess the safety and efficacy of radiation-sterilized allografts of iliac bone and fascia lata from cadaver specimens to repair skull base defects after transsphenoidal surgery. Methods : Between May 2009 and January 2010, 31 consecutive patients underwent endonasal transsphenoidal surgery and all patients received sellar reconstruction using allografts following tumor removal. The allografts were obtained from the local tissue bank and harvested from cadaver donors. The specimens used in our approach were tensor fascia lata and the flat area of iliac bone. For preparation, allografts were treated with gamma irradiation after routine screening by culture, and then stored at $-70^{\circ}C$. Results : The mean follow-up period after surgery was 12.6 months (range, 7.4-16 months). Overall, postoperative cerebrospinal fluid (CSF) leaks occurred in three patients (9.7%) and postoperative meningitis in one patient (3.2%). There was no definitive evidence of wound infection at the routine postoperative follow-up examination or during re-do surgery in three patients. Postoperative meningitis in one patient was improved with the use of antibiotics and prolonged CSF diversion. Conclusion : We suggest that allograft materials can be a feasible alternative to autologous tissue grafts for sellar reconstruction following transsphenoidal surgery under selected circumstances such as no or little intraoperative CSF leaks.

역컴파일링 기법을 이용한 가상기계 코드 실행 시스템 (Executing System of Virtual Machine Code using Decompiling Method)

  • 안덕기;이창환;오세만
    • 정보처리학회논문지A
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    • 제14A권2호
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    • pp.91-98
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    • 2007
  • 일반적으로 가상기계 플랫폼은 컴파일러와 어셈블리, 가상기계로 구성되어 있다. 가상기계 코드의 설계는 가상기계 플랫폼을 만드는데 필수적이며, 설계 결과물인 가상기계 코드의 검증은 매우 중요하다. 가상기계 코드의 검증과 코드의 실행을 위해서는 가상기계 코드의 실행 시스템을 구현하는 것이 필요하고, 컴파일링 기법과 인터프린팅 기법, 역컴파일링 기법으로 실행 시스템을 만들 수 있다. 본 논문에서는 가상기계 코드 실행을 위한 3가지 방법 중에서 역컴파일링 기법을 이용한 가상기계 코드 실행 시스템을 제안 및 구현한다. 제안하는 실행 시스템을 가상기계 코드로 EVM(Embedded Virtual Machine)의 중간언어인 SIL(Standard Intermediate Language)을 사용하였고, 이를 통해 역컴파일의 유용성을 확인하였다. 또한 제안한 역컴파일링 기법은 가상기계 플랫폼을 구현할 때 발생할 수 있는 오류의 최소화에 사용할 수 있다.

방사선학과 학생이 교외 임상실습에서 경험한 스트레스 요인에 관한 연구 (A Study on Stress Factors Radiology Students Experience During Outdoor Clinical Training)

  • 김정훈;강세식;김창수
    • 대한방사선기술학회지:방사선기술과학
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    • 제36권3호
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    • pp.201-208
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    • 2013
  • 방사선(학)과 학생들이 임상실습 시 경험할 수 있는 스트레스 요인을 분석하여 효율적인 임상실습교육과 개선에 도움을 주고자 본 연구를 진행하였다. 연구방법은 부산 경남 지역 5개 대학 방사선학과 학생들 중 임상실습을 경험한 학생을 대상으로 설문을 실시하였다. 그 결과, 5가지 스트레스 항목 중 환경요인이 가장 높은 스트레스 원으로 나타났으며, 다음으로 이상과 가치, 역할 및 활동 순으로 나타났다. 또한 일상에서 느끼는 스트레스보다 임상실습 시 느끼는 스트레스가 더 높게 나타났다. 본 연구 결과를 토대로 임상실습 스트레스를 줄인다면 학생들의 임상실습 만족도를 높이는데 기여하는 것은 물론 임상실습의 질 또한 향상시킬 수 있을 것으로 판단된다.

국내 대학생의 패션의류제품 업사이클링 인식도 조사 (Upcycling Awareness Research Fashion Clothing Goods for Korean University Students)

  • 최세린;최은희;도월희
    • 한국의류산업학회지
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    • 제16권1호
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    • pp.111-117
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    • 2014
  • This study investigated the perception of upcycling for sensitive university students in regards to fashion trends. It was conducted through questionnaires delivered to male and female college students who majored in clothing related subjects from June 2012 to June 2013 in Gwangju. The contents for this research are general details, seriousness of environmental resources, awareness of upcycle, and purchasing intention for upcycling products. Most of the respondents said that recycling of resources is absolutely necessary due to the impact of environmental pollution and the consumption of resources. On the contrary, the experience of the respondents which purchased recycling products was low. The awareness of also was low, upcycling, internal upcycling brands and internal consumption groups, however, the decisions for subsequent purchases were positive. The reasons for the unsatisfactory of upcycling of products were due to quality and price. The reaction for many upcycle goods had the best question response rate in daily supplies part; subsequently, this study suggested 3 items, apparel products, fashion accessories, and daily supplies. In addition, a group which contains people who expend more for clothing had a lower awareness of upcycle and purchasing intention than other groups that contained some people who expend less. The results of the study can be used in data that recognize the necessary of upcycle education for university students, and develop educational programs. It can also encourage the purchase of upcycle products that reduce the consumption of resources.

반복자연유산 환자에서 Homocysteine과 Methylenetetrahydrofolate Reductase 돌연변이의 상관관계에 대한 분석 (The Analysis of Interrelationship between Homocysteine and Methylenetetrahydrofolate Reductase Mutation in Patients with Recurrent Spontaneous Abortion)

  • 남윤성;차광렬;김남근;강명서;김세현;오도연
    • Clinical and Experimental Reproductive Medicine
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    • 제29권3호
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    • pp.187-193
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    • 2002
  • Objective : To analyze the interrelationship between homocysteine and methylenetetrahydrofolate reductase (MTHFR) mutation in patients with recurrent spontaneous abortion. Material and Method: Homocysteine and MTHFR mutation were tested by fluorescent polarizing immunoassay and PCR-RFLP method, respectively. Results: In patients with homocysteine level less than 5 ?mol/L, there was no case of normal group but there were four cases of heterozygosity and one case of homozygosity. In patients with homocysteine level 5$\sim$10 ? mol/L, the number of normal, heterozygosity and homozygosity group were eleven, eighteen and eight, respectively. In patients with homocysteine level $10{\sim}15$ ? mol/L, the number of normal, heterozygosity and homozygosity group were four, one and one, respectively. In patients with homocysteine level more than 15 ? mol/L, there was no case of normal and heterozygosity group but there were two cases of homozygosity. Conclusions: Hyperhomocysteinemia due to MTHFR mutation is a cause of recurrent spontaneous abortion. And there was a significant relationship between homocysteine and MTHFR mutation.

경기북부지역 지하수의 지역별 미량무기물질 함유실태 조사연구 (A survey on the content of inorganic materials in ground-water of Northern Gyeonggi area)

  • 오조교;손진석;배용수;정은희;박진호;황선민;정연훈
    • 환경위생공학
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    • 제19권1호
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    • pp.8-16
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    • 2004
  • Ground water samples were collected from residential, green" industrial, landfill and mining area and analyzed for 23 inorganics from March to October in 2002. The results are as follows; 1. The detection frequencies of inorganic materials such as As, Ba, B, Mo, Ca, Mg, Al, Cu, Fe, Mn, Zn, Na, K were 7.2∼77.1% which showed that these inorganics were detected in many ground waters. 2. The detection frequencies of 7 inorganics(Cd, Cr, Ph, Se, Sb, Be, Tl) were commonly low at ranging from 0.42 to 2.5%. These inorganics were contained in ground water partially. 3. Three inorganics such as Hg, Ni, Ag were not detected in any ground-water samples. 4. Compared to the other areas, ground water samples from landfill area contained greater concentration in many inorgainics and then showed higher levels in industral, residential, mining and green area in order. 5. Compared to noted concentrations of bottled water, inorganic minerals including Ca, Mg, Na, K related to taste were fluent in target samples. The results showed that the average concentrations of Mg, Na, K were 4.0 mg/l, 14.7 mg/l, 1.5 mg/l respectively. 6. The concentrations of inorganics such as Sb, Ba, Mo, Be, Tl, K in some ground water samples exceeded the water quality standards of WHO, the US and UK. Especially, Ba, Mo, K also showed relatively high detection frequencies so these inorganics need to be considered as analytes in Korea Drinking Water Regulation in further study.