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Morphological, Molecular, and Biochemical Characterization of Monounsaturated Fatty Acids-Rich Chlamydomonas sp. KIOST-1 Isolated from Korea

  • Jeon, Seon-Mi;Kim, Ji Hyung;Kim, Taeho;Park, Areumi;Ko, Ah-Ra;Ju, Se-Jong;Heo, Soo-Jin;Oh, Chulhong;Affan, Md. Abu;Shim, Won-Bo;Kang, Do-Hyung
    • Journal of Microbiology and Biotechnology
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    • v.25 no.5
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    • pp.723-731
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    • 2015
  • Microalgae hold promise as producers of sustainable biomass for the production of biofuels and other biomaterials. However, the selection of strains with efficient and robust production of desirable resources remains challenging. In this study, we isolated a green microalga from Korea and analyzed its morphological, molecular, and biochemical characteristics. Microscopic and phylogenetic analyses demonstrated that the isolate could be classified into the genus Chlamydomonas, and we designated the isolate Chlamydomonas sp. KIOST -1. Compositions of protein, lipid, and carbohydrate in the microalgal cells were estimated to be 58.8 ± 0.2%, 22.7 ± 1.2%, and 18.5 ± 1.0%, respectively. Similar to other microalgae belonging to Chlorophyceae, the dominant amino acid and monosaccharide in Chlamydomonas sp. KIOST-1 were glutamic acid and glucose. On the other hand, the proportions of saturated fatty acids, monounsaturated fatty acids, and polyunsaturated fatty acids clearly differed from other species in the genus Chlamydomonas, and monounsaturated fatty acids accounted for a large portion (41.3%) of the total fatty acids in the isolate. Based on these results, Chlamydomonas sp. KIOST-1 has advantageous characteristics for biomass production.

The Analysis of Methylenetetrahydrofolate Reductase Mutation in Recurrent Spontaneous Abortion (반복자연유산에서 Methylenetetrahydrofolate Reductase 돌연변이에 대한 분석)

  • Nam, Yoon-Sung;Cha, Kwang-Yul;Kim, Nam-Keun;Kim, Sun-Hee;Lim, Jin-Woo;Kang, Geum-Duk;Kang, Myung-Seo;Kim, Se-Hyun;Oh, Do-Yeun
    • Clinical and Experimental Reproductive Medicine
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    • v.28 no.3
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    • pp.247-253
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    • 2001
  • Objective: To analyze the methylenetetrahydrofolate reductase (MTHFR) mutation in patients with recurrent spontaneous abortion. Material and Method: The blood samples of patients with recurrent spontaneous abortion were tested by PCR-RFLP method. Results: Of 51 cases of study group, 14 (27.5%) were normal, 25 (49.0%) were heterozygosity, and 12 (23.5%) were homozygosity. Of 58 cases of control group, 20 (34.5%) were normal, 30 (51.7%) were heterozygosity, and 8 (13.8%) were homozygosity. But the difference between two groups was not significant (p=0.190). Conclusion: Hyperhomocysteinemia due to MTHFR mutation is a cause of recurrent spontaneous abortion. Therefore, the study for MTHFR mutation should be included in the workup of recurrent spontaneous abortion.

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ANALYSIS OF THE EFFECT OF HYDROXYL GROUPS IN SILICON DIRECT BONDING USING FT-IR (규소 기판 접합에 있어서 FT-IR을 이용한 수산화기의 영향에 관한 해석)

  • Park, Se-Kwang;Kwon, Ki-Jin
    • Journal of Sensor Science and Technology
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    • v.3 no.2
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    • pp.74-80
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    • 1994
  • Silicon direct bonding technology is very attractive for both silicon-on-insulator devices and sensor fabrication because of its thermal stress free structure and stability. The process of SDB includes hydration of silicon wafer and heat treatment in a wet oxidation furnace. After hydration process, hydroxyl groups of silicon wafer were analyzed by using Fourier transformation-infrared spectroscopy. In case of hydrophilic treatment using a ($H_{2}O_{2}\;:\;H_{2}SO_{4}$) solution, hydroxyl groups are observed in a broad band around the 3474 $cm^{-1}$ region. However, hydroxyl groups do not appear in case of diluted HF solution. The bonded wafer was etched by using tetramethylammonium hydroxide etchant. The surface of the self etch-stopped silicon dioxide is completely flat, so that it can be used as sensor applications such as pressure, flow and acceleration, etc..

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Effect of Degraded Al-doped ZnO Thin Films on Performance Deterioration of CIGS Solar Cell (고온 및 고온고습 환경 내에서 ZnO:Al 투명전극의 열화가 CIGS 박막형 태양전지의 성능 저하에 미치는 영향)

  • Kim, Do-Wan;Lee, Dong-Won;Lee, Hee-Soo;Kim, Seung-Tae;Park, Chi-Hong;Kim, Yong-Nam
    • Journal of the Korean Ceramic Society
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    • v.48 no.4
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    • pp.328-333
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    • 2011
  • The influence of Al-doped ZnO (AZO) thin films degraded under high temperature and damp heat on the performance deterioration of Cu(In,Ga)$Se_2$ (CIGS) solar cells was investigated. CIGS solar cells with AZO/CdS/CIGS/Mo structure were prepared on glass substrate and exposed to high temperature ($85^{\circ}C$) and damp heat ($85^{\circ}C$/85% RH) for 1000 h. As-prepared CIGS solar cells had 64.91% in fill factor (FF) and 12.04% in conversion efficiency. After exposed to high temperature, CIGS solar cell had 59.14% in FF and 9.78% in efficiency, while after exposed to damp heat, it had 54.00% in FF and 8.78% in efficiency. AZO thin films in the deteriorated CIGS solar cells showed increases in resistivity up to 3.1 times and 4.4 times compared to their initial resistivity after 1000 h of high temperature and damp heat exposure, respectively. These results can be explained by the decreases in carrier concentration and mobility due to diffusion or adsorption of oxygen and moisture in AZO thin films. It can be inferred that decreases in FF and conversion efficiency were caused by an increase in series resistance, which resulted from an increase in resistivity of AZO thin films degraded under high temperature and damp heat.

Clinical Efficacy of Radiation-Sterilized Allografts for Sellar Reconstruction after Transsphenoidal Surgery

  • Kim, Se-Jin;Jeon, Chi-Man;Kong, Doo-Sik;Park, Kwan;Kim, Jong-Hyun
    • Journal of Korean Neurosurgical Society
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    • v.50 no.6
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    • pp.503-506
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    • 2011
  • Objective : The aim of this study was to assess the safety and efficacy of radiation-sterilized allografts of iliac bone and fascia lata from cadaver specimens to repair skull base defects after transsphenoidal surgery. Methods : Between May 2009 and January 2010, 31 consecutive patients underwent endonasal transsphenoidal surgery and all patients received sellar reconstruction using allografts following tumor removal. The allografts were obtained from the local tissue bank and harvested from cadaver donors. The specimens used in our approach were tensor fascia lata and the flat area of iliac bone. For preparation, allografts were treated with gamma irradiation after routine screening by culture, and then stored at $-70^{\circ}C$. Results : The mean follow-up period after surgery was 12.6 months (range, 7.4-16 months). Overall, postoperative cerebrospinal fluid (CSF) leaks occurred in three patients (9.7%) and postoperative meningitis in one patient (3.2%). There was no definitive evidence of wound infection at the routine postoperative follow-up examination or during re-do surgery in three patients. Postoperative meningitis in one patient was improved with the use of antibiotics and prolonged CSF diversion. Conclusion : We suggest that allograft materials can be a feasible alternative to autologous tissue grafts for sellar reconstruction following transsphenoidal surgery under selected circumstances such as no or little intraoperative CSF leaks.

Executing System of Virtual Machine Code using Decompiling Method (역컴파일링 기법을 이용한 가상기계 코드 실행 시스템)

  • Ahn, Duk-Ki;Yi, Chang-Hwan;Oh, Se-Man
    • The KIPS Transactions:PartA
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    • v.14A no.2
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    • pp.91-98
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    • 2007
  • Generally, virtual machine platform is composed of a compiler, an assembler, and VM(Virtual Machine). To develop it, the design of VMC(Virtual Machine Code) is an essential task. And it is very important to verify the virtual machine platform. To do this and furthermore to execute VMC, it needs to implement VMC execution system using compiling method, interpreting method, or decompiling method. In this paper, we suggested and implemented the executing system of VMC using decompiling method out of three methods to execute the VMC. In our implementation, the VMC is SIL(Standard Intermediate Language) that is an intermediate code of EVM(Embedded Virtual Machine). Actually, we verified the usefulness of the decompiling method. And the decompiling method suggested in this paper can be used to minimize the mistake in developing Virtual machine platform.

A Study on Stress Factors Radiology Students Experience During Outdoor Clinical Training (방사선학과 학생이 교외 임상실습에서 경험한 스트레스 요인에 관한 연구)

  • Kim, Jung-Hoon;Kang, Se-Sik;Kim, Chang-Soo
    • Journal of radiological science and technology
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    • v.36 no.3
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    • pp.201-208
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    • 2013
  • This study aimed at analyzing stress factors of radiography students may experience during their clinical training, and thus at providing assistance in improving efficiency and quality of clinical training. As for the method of study, a questionnaire survey was conducted targeting radiography students from five universities in Busan and Gyeongsangnam-do regions who had clinical training experiences. The result of the survey showed that, among five stress factors, environmental factor was the highest source of stress, followed by ideals, values, roles and activity. In addition, it was found that the level of stress experienced during clinical training was higher than the level of stress in daily life. If the stress of clinical training can be reduced based on the results of this study, it will not only contribute to improving students' level of satisfaction with clinical training, but also enhance the quality of clinical training.

Upcycling Awareness Research Fashion Clothing Goods for Korean University Students (국내 대학생의 패션의류제품 업사이클링 인식도 조사)

  • Choi, Se-Lin;Choi, Eun-Hee;Do, Wol-Hee
    • Fashion & Textile Research Journal
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    • v.16 no.1
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    • pp.111-117
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    • 2014
  • This study investigated the perception of upcycling for sensitive university students in regards to fashion trends. It was conducted through questionnaires delivered to male and female college students who majored in clothing related subjects from June 2012 to June 2013 in Gwangju. The contents for this research are general details, seriousness of environmental resources, awareness of upcycle, and purchasing intention for upcycling products. Most of the respondents said that recycling of resources is absolutely necessary due to the impact of environmental pollution and the consumption of resources. On the contrary, the experience of the respondents which purchased recycling products was low. The awareness of also was low, upcycling, internal upcycling brands and internal consumption groups, however, the decisions for subsequent purchases were positive. The reasons for the unsatisfactory of upcycling of products were due to quality and price. The reaction for many upcycle goods had the best question response rate in daily supplies part; subsequently, this study suggested 3 items, apparel products, fashion accessories, and daily supplies. In addition, a group which contains people who expend more for clothing had a lower awareness of upcycle and purchasing intention than other groups that contained some people who expend less. The results of the study can be used in data that recognize the necessary of upcycle education for university students, and develop educational programs. It can also encourage the purchase of upcycle products that reduce the consumption of resources.

The Analysis of Interrelationship between Homocysteine and Methylenetetrahydrofolate Reductase Mutation in Patients with Recurrent Spontaneous Abortion (반복자연유산 환자에서 Homocysteine과 Methylenetetrahydrofolate Reductase 돌연변이의 상관관계에 대한 분석)

  • Nam, Yoon-Sung;Cha, Kwang-Yul;Kim, Nam-Keun;Kang, Myung-Seo;Kim, Se-Hyun;Oh, Do-Yeon
    • Clinical and Experimental Reproductive Medicine
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    • v.29 no.3
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    • pp.187-193
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    • 2002
  • Objective : To analyze the interrelationship between homocysteine and methylenetetrahydrofolate reductase (MTHFR) mutation in patients with recurrent spontaneous abortion. Material and Method: Homocysteine and MTHFR mutation were tested by fluorescent polarizing immunoassay and PCR-RFLP method, respectively. Results: In patients with homocysteine level less than 5 ?mol/L, there was no case of normal group but there were four cases of heterozygosity and one case of homozygosity. In patients with homocysteine level 5$\sim$10 ? mol/L, the number of normal, heterozygosity and homozygosity group were eleven, eighteen and eight, respectively. In patients with homocysteine level $10{\sim}15$ ? mol/L, the number of normal, heterozygosity and homozygosity group were four, one and one, respectively. In patients with homocysteine level more than 15 ? mol/L, there was no case of normal and heterozygosity group but there were two cases of homozygosity. Conclusions: Hyperhomocysteinemia due to MTHFR mutation is a cause of recurrent spontaneous abortion. And there was a significant relationship between homocysteine and MTHFR mutation.

A survey on the content of inorganic materials in ground-water of Northern Gyeonggi area (경기북부지역 지하수의 지역별 미량무기물질 함유실태 조사연구)

  • 오조교;손진석;배용수;정은희;박진호;황선민;정연훈
    • Journal of environmental and Sanitary engineering
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    • v.19 no.1
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    • pp.8-16
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    • 2004
  • Ground water samples were collected from residential, green" industrial, landfill and mining area and analyzed for 23 inorganics from March to October in 2002. The results are as follows; 1. The detection frequencies of inorganic materials such as As, Ba, B, Mo, Ca, Mg, Al, Cu, Fe, Mn, Zn, Na, K were 7.2∼77.1% which showed that these inorganics were detected in many ground waters. 2. The detection frequencies of 7 inorganics(Cd, Cr, Ph, Se, Sb, Be, Tl) were commonly low at ranging from 0.42 to 2.5%. These inorganics were contained in ground water partially. 3. Three inorganics such as Hg, Ni, Ag were not detected in any ground-water samples. 4. Compared to the other areas, ground water samples from landfill area contained greater concentration in many inorgainics and then showed higher levels in industral, residential, mining and green area in order. 5. Compared to noted concentrations of bottled water, inorganic minerals including Ca, Mg, Na, K related to taste were fluent in target samples. The results showed that the average concentrations of Mg, Na, K were 4.0 mg/l, 14.7 mg/l, 1.5 mg/l respectively. 6. The concentrations of inorganics such as Sb, Ba, Mo, Be, Tl, K in some ground water samples exceeded the water quality standards of WHO, the US and UK. Especially, Ba, Mo, K also showed relatively high detection frequencies so these inorganics need to be considered as analytes in Korea Drinking Water Regulation in further study.