• Title/Summary/Keyword: $C_{50}$

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CHANGES IN SOME PHYSIOLOGICAL PARAMETERS OF ALBINO RATS AT DIFFERENT AMBIENT TEMPERATURES

  • Hassanin, S.H.;Khali, F.A.;Abd-Elaziz, A.M.S.;EL-Sobhy, H.E.
    • Asian-Australasian Journal of Animal Sciences
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    • v.7 no.4
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    • pp.471-474
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    • 1994
  • Five experimental groups with five adult male rats in each, were exposed to 20, 35, 40 and $45^{\circ}C$ air temperature for 50-70 minutes, and to $50^{\circ}C$ for 30-50 minutes, respectively. Food and drinking water were not permitted during the exposure. Blood samples were obtained by heart puncture immediately after the thermal treatment. All the rats were hyperthermic (p<0.01) as compared to the controls ($20^{\circ}C$). Hyperthermia was associated with hypoglycemia which was significant (p<0.01) at 45 and $50^{\circ}C$ exposures. Plasma levels of GOT and GPT declined at 35 and $40^{\circ}C$ reaching the lowest (p<0.05) level at $45^{\circ}C$, while at $50^{\circ}C$ GOT level was elevated by 45% but GPT was normal as compared to the controls. Differences between groups were significant (p<0.01) for GOT and insignificant for GPT. Hematocrit value increased significantly (p<0.01) at 45 and $50^{\circ}C$, indicating hemoconcentration. It could be concluded that severe heat stress (45 and $50^{\circ}C$) resulted in critical hyperthermia, hypoglycemia, disturbed liver function, body dehydration, and hemoconcentration leading to death.

Varietal Difference of Salt Tolerance during Germination in Italian Ryegrass (이탈리안라이그래스 발아기 내염성의 품종간 차이)

  • 이강수;최선영
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.40 no.4
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    • pp.413-419
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    • 1995
  • This study was conducted to find out the suitable NaCl concentration and temperature for investigating the varietal difference of salinity tolerance in Italian ryegrass. Seeds of 20 cultivars including 9 diploid and 11 tetraploid were exposed to eight levels of NaCl concentration ranging from 0 to 350mM under three temperature levels of 15, 20 and $25^{\circ}C$. The NaCl concentration(C50%) inhibited germination of 50% of the viable seeds for the cultivars at 15$^{\circ}C$ was 354mM, and those at 2$0^{\circ}C$ and $25^{\circ}C$ were 342mM and 325mM, respectively. There were significant correlations among C50% at three levels of temperature, and correlation coefficient for C50% between 2$0^{\circ}C$ and 15$^{\circ}C$ was higher than that between $25^{\circ}C$ and 15$^{\circ}C$ or 2$0^{\circ}C$. C50% was positively correlated only with germination speed at 10 days after sowing when the plants were exposed to 350mM NaCl at 2$0^{\circ}C$. For the germination speed at 10 days after sowing in 350mM NaCl at 2$0^{\circ}C$, the cultivars used could be classified into three groups. Wase Aoba, Bettina, E. K -11, Tetrone, Lira Sand, Tetra Florum and Billiken belonged to the tolerant group, and Tuchi Was, Wase Yutaka, Sakura Wase, Magnolia, Limella and Delita were as the semi-susceptible group, and Atalja, Barmultra, Ajax, Liberta, LM-16, Elving and Wilo were as the susceptible group.

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Antioxidant and Antigenotoxic Effects of Sansuyu Fruit (Corni fructus) Extracted with Water at Different Temperatures (추출 온도에 따른 산수유의 항산화 활성 및 항유전독성 효과 비교)

  • Lee, Min-Hee;Kim, Jung-Mi;Park, Eun-Ju
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.40 no.2
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    • pp.149-155
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    • 2011
  • The objective of this study was to evaluate the antioxidant and antigenotoxic activities of sansuyu fruit (Corni fructus, CF) at temperatures of $25^{\circ}C$, $50^{\circ}C$, and $90^{\circ}C$ using a water extraction method. Total phenolic content (TPC), DPPH radical-scavenging activity (RSA), and superoxide dismutase (SOD)-like activity, and ORAC (Oxygen radical absorbance capacity) values were determined. Also the antigenotoxicity of CF was determined by measuring inhibitory effects of $H_2O_2$ induced DNA damage in human leukocytes using the comet assay. The TPC in the CF extracts was 4.2, 4.6, and 5.5 g/100 g GAE in $25^{\circ}C$, $50^{\circ}C$, and $90^{\circ}C$, respectively. The DPPH RSA of the CF extracts increased in a dose-dependent manner over the range of $50\sim1000\;{\mu}g$/mL in all temperatures and the $SC_{50}$ of DPPH RSA of the CF extracts were not significantly different at different extraction temperatures. The $SC_{50}$ of SOD-like was the highest in CF extracted at $25^{\circ}C$ (1.1 mg/mL) followed by $90^{\circ}C$ (1.2 mg/mL) and $50^{\circ}C$ (1.3 mg/mL). The ORAC values of the CF extracts were not significantly different in low concentration ($10\;{\mu}M$/mL) and was in order of $25^{\circ}C$ ($5.7\;{\mu}M$ TE)< $90^{\circ}C$ ($6.2\;{\mu}M$ TE)< $50^{\circ}C$ ($8.5\;{\mu}M$ TE) in high concentration ($50\;{\mu}M$/mL). $200\;{\mu}M$ $H_2O_2$ induced DNA damages in human leukocytes were significantly reduced by the pretreatment with the CF extracts. These results suggest that sansuyu fruit (Corni fructus) can be used as a natural source for antioxidant activities and as antigenotoxic agents regardless of the water extraction temperature.

Characteristics of Acoustic Indicators Evaluating Speech Intelligibility in Korean Elementary School Classrooms (초등학교 일반교실의 음향성능 실태측정 및 평가지표 특성 고찰)

  • Lee, Seong-Bok;Kim, Myung-Jun;Yang, Hong-Seok
    • Transactions of the Korean Society for Noise and Vibration Engineering
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    • v.25 no.7
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    • pp.462-469
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    • 2015
  • This study was carried out to examine characteristics of various acoustic indicators evaluating speech intelligibility such as reverberation time(T30), D50, C50 and speech transmission index(STI) in Korean elementary school classrooms. Results showed that mean T30 at middle frequencies(500 Hz to 2000 Hz) measured in 9 classrooms was about 0.75 s, which exceeds a regulation specified on American National Standards(ANSI); 0.60 s. Mean D50, C50 and STI were 60 % to 66 %, +2 dB to +3 dB, and 0.65, respectively. The maximum difference in D50 and C50 according to different receiver points in a classroom was 13 % and 2.5 dB, while the maximum difference in T30 was 0.03 s. Whereas STI measured in classrooms has relatively low correlation with other indicators, correlation between D50 and C50 was high, R2=.9964. In addition, T30 and C50 were fitted well as logarithmic regression curve with R2=.9610. It was +3.73 dB in C50 and 68 % in D50 which are the value corresponding to 0.60 s in T30 on this curve.

Design of Electrodes of the Tapered Region of the Traveling-Wave Optical Modulator: SOR Method (진행파 광변조기의 Taper영역 전극 설계 : SOR기법)

  • 정석원;김창민
    • Korean Journal of Optics and Photonics
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    • v.7 no.1
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    • pp.77-86
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    • 1996
  • We employ the SOR method to analyze CPW electrodes of Ti:$LiNbO_3$ Mach-Zehnder traveling-wave optical modulator in the tapered region of an optical modulator. In the tapered region of the modulator, we design the electrodes satisfying $Z_c=50{\Omega}$. In the modulation region, we find two find sets of design parameters. One set is for $Z_c=50{\Omega}$, $n_{eff}=2.26$ and the another is for $n_{eff}=2.2$, $Z_c=47.7{\Omega}$.

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Effects of Temperature and Shading on Germination and Early Growth in Pimpinella brachycarpa (온도와 차광이 참나물 발아 및 초기 생장 특성에 미치는 영향)

  • Jeon, Kwon Seok;Song, Ki Seon;Choi, Kyu Seong;Kim, Chang Hwan;Park, Yong Bae;Kim, Jong Jin
    • Journal of Bio-Environment Control
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    • v.23 no.4
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    • pp.342-348
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    • 2014
  • This study was carried out to determine the effects of temperature and shading on germination and early growth of Pimpinella brachycarpa. Experiment was performed by temperature controls ($5^{\circ}C$, $10^{\circ}C$, $15^{\circ}C$, $20^{\circ}C$, $25^{\circ}C$, and $30^{\circ}C$) and shading treatments (control, 35% shading, 50% shading, and 75% shading). Seed pre-treatment before the germination experiment was carried out by temperature (with low temperature and wetting treatments for 0day, 20days, and 40days). Seeds of P. brachycarpa were, 20days of seed pre-treatment, germinated well at $5^{\circ}C$ and the more temperature went up, the more germination rate went down. As a result of surveying shading treatments, the height, leaf area, leaf length, leaf width etc were the highest (7.3cm, $12.8cm^2$, 5.4cm, and 2.3cm, respectively) under 50% shading. Specially, the root was grown well under 35% and 50% shading. Fresh weight of shoot (leaves+stem) was the highest under 50% and 75% shading and dry weight of shoot (leaves+stem) was the highest under 35% and 50% shading. As a result of surveying the whole experiment, it is concluded that the production of P. brachycarpa seedling is more effective under 50% shading.

Solids Loss with Water Uptake during Soaking of Soybeans (대두의 침지과정중 침출액의 성분변화)

  • Lee, Yung-Heon;Jung, Hae-Ok;Rhee, Chong-Ouk
    • Korean Journal of Food Science and Technology
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    • v.19 no.6
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    • pp.492-498
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    • 1987
  • Dried soybeans (varieties: Saeal, Kwanggyo, Tanyob) took up water rapidly for first 3hr followed by a slower rate of uptake. The beans took up an equal weight of water (100% hydration)after approximately 3.5hr at $50^{\circ}C$, 5hr at $30^{\circ}C$ and 7.5hr at $20^{\circ}C$ respectively. pH of the soaking solutions decreased during the soaking period. This was undoubtedly caused by the ionization of the cellular components resulting in increased levels of hydrogen ions in the liquor. Soluble solids were leached out of the beans at fairly steady rate throughout the hydration and the amount was greater with higher temperature. This amounted to 0.4-0.7g at $20^{\circ}C$ and 10.2-15.0g at $50^{\circ}C$ per 100g soybeans. Temperature was the most important factor in determining the rate of water absorption and of solid losses. Of the total solids lost, 12-25% was protein. The proportion of protein loss increased as the soaking time and temperature increase. Amount of protein loss was 80-200mg at $20^{\circ}C$ and 440-480mg at $50^{\circ}C$ after 24hr soaking per 100g soybeans. About 5% of soluble sugars, including fructose, sucrose, raffinose, and stachyose, was removed from the beans after 24hr soaking at $20^{\circ}C$.

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The Effect of Nutritional Balance between Carbon and other Nutrient Sources on the Growth of Sporobolomyces holsaticus (탄소원과 다른 영양원간의 영양균형이 Sporobolomyces holsaticus의 균체생육도에 미치는 영향)

  • Park, Wan-Soo;Koo, Young-Jo;Shin, Dong-Hwa;Min, Byung-Yong
    • Korean Journal of Food Science and Technology
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    • v.15 no.1
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    • pp.56-61
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    • 1983
  • Direct production of biomass from starch using amylolytic yeast, Sporobolomyces holsaticus FRI Y-5 was studied with varying the ratios between carbon and other nutrient sources in the medium. It was investigated under condition of constant C/P and C/S ratio to influence the initial concentration of starch $(S_o)$ and C/N ratio on its growth which is described as the specific growth rate $({\mu})$, cell yield (Y), the maximum concentration of cell $(X_m)$, and productivity (P). They were very dependent on both $S_o$ and C/N ratio. The form of the relationship between and ${\mu}$ and $S_o$ was observed to be similar to saturation kinetics at C/N = 100 but presented substrate inhibition at other C/N ratios. As $S_o$ was changed from 22.5 to 90 g/l, Y was observed to vary with C/N ratios but seemed to decrease as a wholes. $X_m$ was linearly related to $S_o$ at more than C/N = 50 but at less than C/N = 10 substrate inhibition was presented. P increased suddenly to $S_o$ = 45 g/l and then changed decreasingly at less than C/N = 50, but at more than C/N = 100 it changed increasingly. The effect of C/P ratio and C/S ratio on the yeast growth was also investigated at constant $S_o$ and C/N ratio. ${\mu}$ was dependent on C/P and C/S ratios, but Y, independent on them. But $X_m$ was reliant upon C/P ratio but not upon C/S ratio.

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Effect of Vitamin C Supplementation on the Performance and Eggshell Quality of Layers (비타민 C 첨가가-산란계의 생산성과 난각품질에 미치는 영향)

  • 백인기;임희석;남궁환
    • Korean Journal of Poultry Science
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    • v.29 no.2
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    • pp.135-140
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    • 2002
  • An experiment was conducted to determine the effects of vitamin C supplementation on the performance and egg quality of layers. A total of 800 ISA Brown layers of 71 weeks old were assigned to one of the following diets containing 0, 3, 10, 50 or 100 ppm LG-vitamin C, 100ppm free ascorbic acid, 100 ppm Rovimix stay C-35 or 50 ppm LG-vitamin C supplemented with 100 ppm Zn methionine chelate. The feeding trial was conducted for flour weeks during the period of May to June when the average ambient temperature was $23.8^{\circ}C$ (5 :00 PM). Each treatment was replicated five times with 20 birds housed in 2 birds cage units. Vitamin C supplementation did not improve the overall performances (egg Production and egg Quality). Birds fed 100 ppm of LG vitamin C had the lowest egg Production while birds fed 100 ppm of Rovimix-35 had the lowest egg weight among birds fed different sources of vitamin C. Although supplementation of vitamin C over 50 ppm increased soft and broken egg Production, supplementation of 10 ppm LG vitamin C showed lowest soft and broken egg Production among all treatments. E99she11 thickness was highest in the treatment of 50 ppm LG vitamin C. In conclusion, supplementation of vitamin C at the level of 3 ~ 100 ppm did not significantly influence the laying performance and eggshell quality of laying hens under normal ambient(20 ~$26^{\circ}C$ ) condition.

Anti-oxidative Activity and Chemical Composition of Various Heracleum moellendorffii Hance Extracts (추출 조건에 따른 어수리의 항산화 활성 및 성분 분석)

  • Bang, Ji-Eun;Choi, Hae-Yeon;Kim, Sun-Im
    • Food Science and Preservation
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    • v.16 no.5
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    • pp.765-771
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    • 2009
  • Anti-oxidative activities of Heracleum moellendorffii Hance extracts were measured after extraction with 50% (v/v) ethanol or water (at $80^{\circ}C$ or $20^{\circ}C$). The total polyphenol content was highest (64.73 mg GAE/g) in extract from 50% ethanol extraction at $80^{\circ}C$. In extracts obtained by water extraction at $80^{\circ}C$ and $20^{\circ}C$, the total polyphenol contents were 39.78 mg GAE/g and 23.17 mg GAE/g, respectively. The antioxidant activities of the ethanol extract at $80^{\circ}C$ were highest, as assessed by DPPH radical scavenging activity (69.14% at 50 ppm), reducing power (OD 0.93), and $ABTS^+$ radical-scavenging activity (85.62% at 1,000 ppm). At $80^{\circ}C$, the antioxidant activity of the 50% (v/v) ethanol extract was 8-20% higher than that of the water extract. Also, the chemical composition of extract from ethanol extraction at $80^{\circ}C$ was analyzed. The levels of moisture, crude protein, crude fat, and crude ash were 6.38%, 4.35%, 0.67%, and 1.96%, (all w/w), respectively. The Na, K, Mg, Zn, Fe, Ca, and P contents of the extract were 53.41, 398.26, 5.80, 0.40, 5.27, 3.56, and 47.17 mg/100 g, respectively. The total levels of polyphenolic compounds and flavonoids in the ethanol extract were 64.73 mg GAE/g and 49.54 mg RE/g.