Food Science and Preservation (한국식품저장유통학회지)

The Korean Society of Food Preservation (한국식품저장유통학회)
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Anti-oxidative Activity and Chemical Composition of Various Heracleum moellendorffii Hance Extracts

추출 조건에 따른 어수리의 항산화 활성 및 성분 분석

  • Bang, Ji-Eun (Department Food and Nutrition, Sookmyung Women's University) ;
  • Choi, Hae-Yeon (Department Food and Nutrition, Sookmyung Women's University) ;
  • Kim, Sun-Im (Nano Bio-resources Center, Sookmyung Women's University)
  • 방지은 (숙명여자대학교 식품영양학과) ;
  • 최해연 (숙명여자대학교 식품영양학과) ;
  • 김순임 (숙명여자대학교 나노바이오소재센터)
  • Published : 2009.10.30
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Abstract

Anti-oxidative activities of Heracleum moellendorffii Hance extracts were measured after extraction with 50% (v/v) ethanol or water (at $80^{\circ}C$ or $20^{\circ}C$). The total polyphenol content was highest (64.73 mg GAE/g) in extract from 50% ethanol extraction at $80^{\circ}C$. In extracts obtained by water extraction at $80^{\circ}C$ and $20^{\circ}C$, the total polyphenol contents were 39.78 mg GAE/g and 23.17 mg GAE/g, respectively. The antioxidant activities of the ethanol extract at $80^{\circ}C$ were highest, as assessed by DPPH radical scavenging activity (69.14% at 50 ppm), reducing power (OD 0.93), and $ABTS^+$ radical-scavenging activity (85.62% at 1,000 ppm). At $80^{\circ}C$, the antioxidant activity of the 50% (v/v) ethanol extract was 8-20% higher than that of the water extract. Also, the chemical composition of extract from ethanol extraction at $80^{\circ}C$ was analyzed. The levels of moisture, crude protein, crude fat, and crude ash were 6.38%, 4.35%, 0.67%, and 1.96%, (all w/w), respectively. The Na, K, Mg, Zn, Fe, Ca, and P contents of the extract were 53.41, 398.26, 5.80, 0.40, 5.27, 3.56, and 47.17 mg/100 g, respectively. The total levels of polyphenolic compounds and flavonoids in the ethanol extract were 64.73 mg GAE/g and 49.54 mg RE/g.

본 연구는 생식으로만 이용하고 있는 어수리 잎을 이용한 기능성 제품을 개발하는데 기초자료를 제공하기 위하여 물과 에탄올을 사용하여($80^{\circ}C$ 50% 에탄올/ $20^{\circ}C$ 50% 에탄올/ $80^{\circ}C$ water/ $20^{\circ}C$ water) 어수리를 추출하여 항산화 활성을 살펴보았다. 항산화성 물질 중 총 폴리페놀 함량은 $80^{\circ}C$에서 50% 에탄올 추출물이 64.73 mg GAE/g으로 가장 높은 함량을 나타내었다. 항산화활성을 측정한 결과 $80^{\circ}C$에서 50% 에탄올로 추출하였을 때 DPPH radical 소거능은 50 ppm에서 69.14%, reducing power는 1000 ppm에서 흡광도 0.93을 나타내었고, ABTS+ radical 소거능은 1000 ppm에서 85.62%의 성을 보여 본 연구에서 사용한 추출방법 중 가장 높은 항산화활성을 보였다. 항산화 활성이 높게 나타난 50% 에탄올 추출물의 일반성분 측정결과 수분함량은 6.38%, 조단백질 함량은 4.35%, 조지방 함량은 0.67%, 그리고 조회분 함량은 1.96%였다. 또한 무기질 분석결과 Na 53.41 mg/100 g, K 396.26 mg/100 g, Mg 5.80 mg/ 100 g, P 47.17 mg/100 g, Zn 0.40 mg/100 g, Fe 5.27 mg/100 g, 그리고 Ca 3.56 mg/100 g으로 나타났다. 기능성 소재 개발의 일환으로 어수리 에탄올 추출물의 항산화관련 성분을 분석한 결과 총 페놀함량과 플라보노이드 함량은 각각 64.73 mg GAE/g과 49.54 mg RE/g로 나타났다. 이상의 결과로 볼 때 어수리를 이용한 기능성식품 제조 시 $80^{\circ}C$에서 50% 에탄올을 이용하여 추출하는 것이 항산화 효과를 높일 수 있는 방법이라고 생각된다.

Keywords

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