• 제목/요약/키워드: $CP_1$

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심근정지후 심근세포의 회복과정과 심근정지용액에 관한 연구 (A Study on the Cardioplegic Solutions and the Cardioplegia of Guinea Pig Heart)

  • 서창국;박소라;박혜수;이영호;강복순
    • The Korean Journal of Physiology
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    • 제22권1호
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    • pp.1-12
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    • 1988
  • The changes in membrane potential and intracellular $Ca^{2+}$ activities along with adenosine triphosphate (ATP) and creatine phosphate (CP) contents of guinea pig hearts were studied during the cardioplegia and recovery phase. The results obtained were summarized as follows: 1) A low concentration of $Ca^{2+}$ in the cardioplegic solution caused significant changes in membrane potential during the cardioplegia and action potential configurations of the first beat after cardioplegia. 2) Intracellular $Ca^{2+}$ activities were decreased significantly during cardioplegia and increased when the cardiac tissue was reperfused with the normal Tyrode solution. The magnitude of increase in intracellular $Ca^{2+}$ activities was larger in the 0.1 mM $Ca^{2+}-cardioplegic$ solution than the 1.8 mM $Ca^{2+}-solution$. 3) ATP and CP contents of cardiac ventricular tissue were decreased during the cardioplegia, regardless of $Ca^{2+}$ concentration in the cardioplegic solutions, and CP contents were recovered with the reperfusion of normal Tyrode solution faster than those of ATP. And, there were no significant differences in the recovery of CP contents with different concentrations of $Ca^{2+}$ in the cardioplegic solutions tested, while the recovery of ATP contents was faster with $15mM\;K^{+},\;0.1mM\;Ca^{2+}$ cardioplegic solution. These results suggest that the $Ca^{2+}$ concentration in the cardioplegic solution is one of major factors influencing the recovery of cardiac tissue from the cardioplegia.

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Structural characteristics of [N(CH3)4]2CdCl4 determined by 1H MAS NMR, 13C CP/ MAS NMR, and 14N NMR

  • Lee, Seung Jin;Lim, Ae Ran
    • 한국자기공명학회논문지
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    • 제19권1호
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    • pp.18-22
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    • 2015
  • The structural geometry of $[N(CH_3)_4]_2CdCl_4$ in a hexagonal phase is studied by $^1H$ MAS NMR, $^{13}C$ CP/MAS NMR, and $^{14}N$ NMR. The changes in the chemical shifts for $^{13}C$ and $^{14}N$ in the hexagonal phase are explained by the structural geometry. In addition, the temperature dependencies of the spin-lattice relaxation time in the rotating frame $T_{1{\rho}}$ for $^1H$ MAS NMR and $^{13}C$ CP/MAS NMR are measured.

Catalytic Oxidation of Phenol Analogues in Aqueous Medium Over Fe/SBA-15

  • Mayani, Suranjana V.;Mayani, Vishal J.;Kim, Sang-Wook
    • Bulletin of the Korean Chemical Society
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    • 제33권9호
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    • pp.3009-3016
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    • 2012
  • This study evaluated the use of iron-impregnated SBA-15 (Fe/SBA-15) as a catalyst for the oxidative degradation of persistent phenol analogues, such as 2-chlorophenol (2-CP), 4-chlorophenol (4-CP), 2-nitrophenol (2-NP), 4-nitrophenol (4-NP) and 2,4,6-trichlorophenol (2,4,6-TCP) in water. The oxidation reactions were carried out with reaction time, concentration of the phenols, amount of the catalysts, reaction temperature, pH of the reaction mixture as the process variables with or without using hydrogen peroxide as the oxidizing agent. The conversion achieved with Fe/SBA-15 at 353 K for 2-CP, 4-CP, 2-NP, 4-NP, 2,4,6-TCP was 80.2, 71.2, 53.1, 62.8, 77.3% in 5h with a reactant to $H_2O_2$ mole ratio of 1:1, and 85.7, 65.8, 61.9, 63.7, 78.1% in the absence of $H_2O_2$, respectively. The reactions followed pseudo first order kinetics. The leachability study indicated that the catalyst released very little iron into water and therefore, the possibility of secondary pollution is negligible.

Isolation and Characterization of Four Carboxypeptidases in Canavalia lineata Cotyledons

  • Yang, Jong-Moon;Rhew, Tae-Hyong;Koh, Suck-Chan;Kwon, Young-Myung
    • BMB Reports
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    • 제28권5호
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    • pp.451-457
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    • 1995
  • Four carboxypeptidases, CP1, CP2, CP3, and CP4 were isolated from the cotyledons of germinating seedlings of Canavalia lineata by sequential chromatography on the following four columns: 1) CM-cellulose, 2) Sephacryl 5-300, 3) Procion red dye, and 4) Sephacryl S-200. A number of properties of the enzymes, such as substrate specificity, molecular weight, optimum pH, thermal stability, have been determined. Enzyme activities were measured using the Cbz(carbobenzoxy)-dipeptides containing phenylalanine at the penultimate position. The $K_m$ values of four carboxypeptidases for Cbz-Phe-Ala were 0.50, 0.65, 1.30, and 1.35 mM, respectively. The inhibition studies indicated that the four carboxypeptidases were all serine type. Each of the carboxypeptidases with molecular weights of 145, 114, 105, and 104 kDa, respectively, had the optimum enzyme activity at pH 5.0~6.0. And they were sensitive to high temperature.

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OFDM의 심벌 타이밍 옵셋 추정을 위한 1심벌 옵셋의 훈련심벌 사용법과 CP 출력조절법 (Symbol timing Offset Estimation for OFDM Using the 1 Symbol Offset Training Symbol and Controled CP Power)

  • 옥윤철;하영호
    • 전자공학회논문지
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    • 제50권12호
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    • pp.3-13
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    • 2013
  • 이 논문에서는 OFDM 시스템의 시간 영역 동기를 위한 2가지 방법을 제안한다. 이것은 심벌타이밍 판정의 모호함의 원인이되는 플레토와 사이드로브를 제거하기 위하여 부 심벌간에 1심벌 옵셋을 두고 타이밍 메트릭을 구하는 방법과 플레토 현상을 경감시킬 수 있는 전력감소지수(Reduction Factor, ${\rho}$), 제동상수(Break Constant, ${\beta}_k$) 그리고 심벌이식깊이(Implant Depth, ${\delta}_I$)등의 파라메터를 사용하여 CP(Cyclic Prefix) 구간의 전력을 수정하는 방법이다. 제안된 2가지 방법은 기존에 제안된 심벌타이밍 동기화기법들과 컴퓨터모의시험을 통해서 성능평가가 이루어졌으며, 제안된 방법들은 다중경로의 레일레이 페이딩채널에서 기존의 시스템에 비해서 더 우수한 통계적 특성을 나타내었다.

마이크로 컨택 프린팅을 이용하여 유기기판 위에 패턴 형성하는 방법

  • 김명수;이다혁;김기보;이진균;오범환;이승걸;박세근
    • 한국진공학회:학술대회논문집
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    • 한국진공학회 2014년도 제46회 동계 정기학술대회 초록집
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    • pp.390-390
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    • 2014
  • Soft-lithography 기술 중의 하나인 Micro-Contact Printing (${\mu}$-CP) 기술은 패턴이 형성된 mold 위에 고분자 물질을 코팅하고 기판과 접촉시켜, 패턴 된 부분만 기판으로 전사시켜 패턴을 형성하는 방법이다. ${\mu}$-CP 기술은 Imprint 방식과 비교하여 잔여물을 제거하기 위한 ashing 공정이 필요 없으며, 상대적으로 패턴이 전사되기 위한 공정 온도와 압력이 낮은 장점이 있다. 한편, 기존의 Photolithography 기술로 유기기판에 패턴을 형성하는 것은 제한이 있으며, 자외선에 의해 유기기판의 특성이 변화될 수 있다. 또한 패턴 형성 후 고분자 패턴을 제거하는 용매가 기판이 손상 받게 된다. 본 실험에서는 poly (1H,1H,2H,2H-perfluorodecyl methacrylate) polymer (PFDMA) films을 패턴 된 poly (dimethylsiloxane) (PDMS) mold 위에 코팅하고 ${\mu}$-CP 기술을 통해 poly (methylmethacrylate) (PMMA), poly (vinyl pyrrolidone) (PVP)등과 같은 유기기판 위에 고분자 패턴 형성을 하였다. 이때 전사 가능한 온도는 상온이며, 압력은 코팅된 PFDMA films이 기판과 접촉될 수 있는 정도만 필요하다. PFDMA가 상온에서 전사 가능한 이유는 유리전이온도가 상온보다 낮기 때문이다. 또한 접촉각을 측정하여 접착력을 계산하면 PFDMA와 기판과의 접착력이 상대적으로 높기 때문이다. PFDMA는 플루오르계 용매에 제거되기 때문에 유기기판의 손상을 최소화 할 수 있다. ${\mu}$-CP 기술을 이용한 PFDMA의 패턴 형성 방법은 물질의 특성으로 flexible 및 organic device 제작에 응용 될 수 있다.

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A Study on the Effect of Conjugated Linoleic Acid on Immunosuppressed Mice with Corticoids

  • 임형진;김진영;임좌진;이병한;박희명;정병현
    • 한국임상수의학회지
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    • 제20권1호
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    • pp.59-65
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    • 2003
  • This study was performed to investigate the immunopotentiative effects of conjugated linoleic acid on mice immunosuppressed by administratin corticoids. Mice were divided into four groups of 8 mice. Two groups (C, CP) were given diet supplemented with 1% linoleic acid (CLA) and the other two groups (L, LP) were given diet supplemented with 1% linoleic acid (LA) instead of CLA. Prednison was administered to two groups (CP, LP) for immune depression. After feeding diets for 3 weeks containing PDS injection for last 1 week. Serum and gut lumen lavage were taken. Measurement of total Ig were executed using sandwich ELISA. Serum levels of IgA, IgG, and IgM showed some trend which groups fed with CLA were higher than groups fed with LA while IgE was reduced in those fed the CLA intake, and groups administered with PDS were lower than groups administered with saline. However, no significant differences were seen in the proportion of total immunoglobulin in serum. In case of secretory IgA, Group C and CP were significantly higher than group L and LP. Especially between CP and LP, it can be seen effects of CLA. In addition that the CLA treated group weighted a significantly lower level than the one's that have not been treated with CLA. These result support the view that CLA potentiate the immune response and prevent immune depression caused by administrating of corticoids. In conclusion, CLA produced a situation favorable for immunopotentiative effects. Thus, the clinical application of CLA is warranted.

Chloroplast genome of the conserved Aster altaicus var. uchiyamae B2015-0044 as genetic barcode

  • Lee, Minjee;Yi, Jae-Sun;Park, Jihye;Lee, Jungho
    • Journal of Species Research
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    • 제10권2호
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    • pp.154-158
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    • 2021
  • An endemic endangered species, Aster altaicus var. uchiyamae (Danyang aster) B2015-0044, is cultivated at the Shingu Botanical Garden, which serves as the ex situ conservation institution for this species. In this work, we sequenced the chloroplast genome of A. altaicus var. uchiyamae B2015-0044. We found that the chloroplast (cp) genome of B2015-0044 was 152,457 base pairs(bps) in size: 84,247 bps of large single copy regions(LSC), 25,007 bps of inverted repeats(IRs), and 18,196 bps of small single copy regions. The B2015-0044 cp genome contains 79 protein-coding genes (PCGs), 4 RNA genes, 29 tRNA genes, and 3 pseudogenes. These results were identical to a previously reported cp genome (Park et al., 2017), except for two sites in introns and three in intergenic spacer (IGS) regions. For the intronic differences, we found that clpP.i1 had a 1-bp small simple repeat (SSR) (T) and petD.i had a 3-bp SSR (ATT). We found 1-bp SSRs in the IGSs of trnT_ggu~psbD and psbZ~trnG_gcc, C and A, respectively. The IGS of(ndhF)~rpl32 had a SNP. Based on our results, the cp genome of the A. altaicus var. uchiyamae can be classified into two genotypes, [C]1-[A]12-[T]12-[ATT]4-C and [C]2-[A]11-[T]11-[ATT]2-A.

A Study on the Application of Cathodic Protection for Anti-Corrosion of Automobile Body

  • Sohn, DaeHong;lee, Yongho;Jang, HeeJin;Cho, SooYeon
    • Corrosion Science and Technology
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    • 제21권1호
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    • pp.1-8
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    • 2022
  • The use of cathodic protection for metals can be achieved by sacrificial anode CP or impressed current CP, or a combination of both. Cathodic protection is a highly effective anti-corrosion technique for submerged metals or metals in soil. But because the non-immersion atmospheric automobile environment is a high resistance environment, it is limited by fundamental cathodic protection. However, the application of cathodic protection to automobiles is attractive because of the possibility of maintaining corrosion resistance while using lower-cost materials. A commercially available product for automobiles that uses both sacrificial anode CP and impressed current CP was tested in a periodic salt spray environment to investigate the performance of the devices. Experimental results show that the metal to be protected has different anti-corrosion effects depending on the distance from the anode of the device, but it is effective for the entire 120 cm long specimen exposed with one anode. The cathodic protection is effective because the conductive tape attached to the anode of the structure to be protected acts as a constant electrolyte in wet and dry conditions. The results show that the entire standard passenger car can be protected by cathodic protection with 4 anodes.

한국재래닭의 육용실용계의 발육 및 육질특성 구명 연구 1. 사료 급여체계에 따른 한국재래닭 육용실용계의 발육능력 (Study on Performance and Meat Characteristics in Korean Native Commercial Chicken I. Study on Performance in Korean Native Commercial Chicken by Feeding System by Feeding System)

  • 강보석;이상진;김상호;김웅배;오봉국
    • 한국가금학회지
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    • 제25권3호
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    • pp.129-136
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    • 1998
  • This experiment was carried out to investigate the effect of feeding system on performance in Korean Native Commercial Chicken. A total 864 birds produced from (Cornish ♂ X (Korean Native Chicken ♂ XRhode Island Red♀)♀ ] crossbreeds in National Livestock Research Institute, for 16 weeks. Feeding system of T1 and T$_2$ were same types from hatch to 8 weeks, starter diets(O~4 weeks, mash, ME 3,100kcal, CP 22.94%), grower diets(4~8 weeks, crumble, ME 3,100kcal, CP 19.31%). Nutrient content of finisher diets of T$_1$(pellet, ME 3,200kcal, CP 20.44%) was higher than T$_2$(mash, ME 3,100kcal, CP 14.88%) in order to improve meat quality for 8~16 weeks. Fertility and hatchability of Korean Native Commercial Chicken was 83.9% and 69.7%, respectively. Viabilities of T$_1$ and T$_2$ at 0, 4, 8, 12 and 16 weeks were 98.8%, 97.9%, 96.5% and 99.1%, 95. 8%, 92.8%, 90.3%, respectively. The viability of 0 to 8 weeks was not significantly in feed treatments, but 12 and 16 weeks was significantly T$_1$ higher than T$_2$(P<0.05). Body weights of T$_1$and T$_2$ at 4, 8, 12 and 16 weeks were 551g, 1,379g, 2,441g, 3,056g and 554g, 1,360g, 2,254g, 2,956g, respectively. The body weight of 0 to 8 weeks was not significantly feed treatments but 12 and 16 weeks was significantly T1 higher than T$_2$(P<0.05). Feed conversion of T$_1$ and T$_2$ to 4, 8,12 and 16 weeks were 1.91, 2.28, 3.34, 4.23 and 1.90, 2.28, 3.53, 4.46, respectively. The feed conversion of 0 to 8 weeks was not significantly feed treatments but 12 and 16 weeks was significantly T$_1$ lower than T$_2$(P<0.05). The ME intake 1 bird per 1 day of T$_1$ and T$_2$were 3S9kcal, 357kca1, respectively, not significantly feed treatments but CP intake were 24.8g, 20.3g, respectively. T$_2$ was lower than T$_1$(P$_1$ and T$_2$were 13,426kca1, 13,819Ykcal, respectively, not significantly feed treatments but CP requirement per kg body weight gain were 928g, 763g, respectively, T$_2$ was lower than T$_1$(P<0.05).

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