• Korean Journal of Soil Science and Fertilizer
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• v.40 no.4
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• pp.242-248
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• 2007

To find a feasibility of utilization of food waste slurry (FWS) generated during composting, FWS was combined with pig manure slurry (PMS) in various ratios and the change of nutrient contents and offensive odor of the combined slurries before and after fermentation were studied. The initial pH was 7.67 for PMS and 8.45 for FWS. However, during the fermentation, pH increased in the combined slurries with the higher FWS rate among the treatments while decreased in thosewith higher PMS rate. EC of each slurry sample showed that the difference among combined slurry samples has been reduced during fermentation and became stabilized in $21{\sim}23dS\;m^{-1}$ after 180 days. After 180 days fermentation, total nitrogen (T-N) decreased. T-N of mixture with a half and more FWS decreased up to 0.1%, less than the critical level (0.3%). The contents of O.M., T-N, phosphorus, calcium and magnesium decreased with fermentation while those of potash and salinity increased. From initial fermentation until 30 days, a lot of $NH_3$, as an offensive odor, was produced. However, it decreased steadily, except in higher PMS rate. In terms of producing $50{\mu}g\;ml^{-1}$ of $NH_3$, the top layer took 30 days after fertilization with FWS only, 45 days for utilized treatment with F75 (25 % of PMS), 75 days for utilized with F50 (50%) and F25 (75%) and 90 days for PMS only, respectively. $RNH_2$ also had similar trend with $NH_3$ but it was produced continuously as long fermentation proceeded. In terms of $RNH_2$, the decrease in concentration up to $50{\mu}g\;ml^{-1}$ were; 45 days for FWS only(F100), 105 days for F75 utilization, 120 daysfor F50, 165 days for F25, respectively. ethyl mercaptan was produced in PMS until 180 days after fertilization but it was not produced in FWS. Sensory tests as an integrated test of offensive odor were also done. FWS showed lower than 1 after 30 days from initial fermentation, while PMS had still offensive odor even up to 180 days from initial fermentation. It is probably affected by the continuous production of ethyl mercaptan and amines. However, considering in decrease T-N content caused by volatilization while offensive odor intensity according to official standard of fertilizer is lower than 2. Further study on controlling offensive odor needs to be done.

• Korean Journal of Microbiology
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• v.51 no.4
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• pp.374-381
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• 2015

Occurrences of coastal dredged materials are ever increasing due to port construction, navigational course maintenance and dredging of polluted coastal sediments. Ocean dumping of the coastal dredged materials has become virtually prohibited as London Treaty will be enacted as of the year 2012. It will be necessary to treat and recycle the dredged materials that may carry organic pollutants and heavy metals in a reasonable and effective process: collection of the dredged materials, liquid and solid separation, and treatment of organic compounds and heavy metals. In this study we have developed a continuous bioreactor system that can treat a mixture of silt and particulate organic matter using a microbial consortium (BM-S-1). The steady-state operation conditions were: pH (7.4-7.5), temperature ($16^{\circ}C$), DO (7.5-7.9), and salt concentration (3.4-3.7%). The treatment efficiencies of SCOD, T-N and T-P of the mixture were 95-96%, 92-99%, and 79-97%. The system was also effective in removal of heavy metals such as Zn, Ni, and Cr. Levels of MLSS during three months operation period were 11,000-19,000 mg/L. Interestingly, there was little sludge generated during this period of operation. The augmented microbial consortium seemed to be quite active in the removal of the organic component (30%) present in the dredged material in association with indigenous bacteria. The dominant phyla in the treatment processes were Proteobacteria and Bacteroidetes while dominant genii were Marinobacterium, Flaviramulus, Formosa, Alteromonadaceae_uc, Flavobacteriaceae_uc. These results will contribute to a development of a successful bioremediation technology for various coastal and river sediments with a high content of organic matter, inorganic nutrients and heavy metals, leading to a successful reuse of the polluted dredged sediments.

• Journal of the Korean earth science society
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• v.29 no.6
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• pp.454-465
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• 2008

Benthic foraminiferal assemblage and AMS radiocarbon dating of core sediments from the northern shelf of the East China Sea were analyzed in order to understand the paleoenvironment and sedimentary environmental changes around the Korean marginal seas since the last glacial maximum (LGM). The core sediments, containing continuous records of the last 16,000 years, reveal a series of well-defined vertical changes in number of species (S), P/T ratio and species diversity (H) as well as foraminiferal assemblage. Such down-core variations display a sharp change at a core depth of approximately 240 cm, which corresponds to ca. 10,000 year B.P. The sediments of the lower part of the core (240${\sim}$560 cm, Zone I), including the well-developed tide-influenced sedimentary structures, are characterized by high abundances of Ammonia beccarii and Elphidium clavatum (s.l.) and low values in number of species, P/T ratio and diversity. These tide-influenced signatures and foraminiferal assemblage characters suggest that the sediments of Zone I were deposited in a coastal environment (water depths of 20${\sim}$30 m) such as tidal estuary with an influence of the paleo-rivers (e.g., old-Huanghe and Yangtze rivers) during the early phase of the sea-level rise (ca. 16,000 to 10,000 years) since the LGM. In contrast, the upper core sediments (0${\sim}$240 cm, Zone II) are characterized by abundant Eilohedra nipponica and Bolivina robusta with a minor contribution of A. ketienziensis angulata and B. marginata. and high values in number of species, P/T ratio and diversity. Based on relative abundance of these assemblage, Zone II can be divided into two subzones (IIa and IIb). Zone IIa is interpreted to be deposited under the inner-to-middle shelf environment during the marine transgression in the early Holocene (after ca. 9,000 yr B.P.) when sea level rapidly increased. The sediments of zone IIb most likely deposited after 6,000 yr B.P. under the outer shelf environment (80${\sim}$100 m water depth), which is similar to modem depositional environments. The muddy sediments of zone IIb were probably transported from the old-Huanghe and Yangtze Rivers during the late Holocene. We suggest that the present-day oceanographic conditions over the Yellow and the East China Seas have been established after ca. 7,000${\sim}$6,000 yr B.P. when the Kuroshio Current began to influence this area.

• Pediatric Infection and Vaccine
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• v.17 no.2
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• pp.122-129
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• 2010

Purpose : Enteroviral infection is a common viral illness in children. We undertook this study in attempt to comprehend the epidemiologic and clinical features of enteroviral infections, particularly EV71 in children. Methods : We enrolled 63 children with enteroviral infection at Severance Children's Hospital in Seoul between May and August 2009. Reverse transcriptase-polymerase chain reaction (RT-PCR) was performed from stool or cerebrospinal fluid samples, which were then tested for enteroviral infection. Viral isolation and serotype identification also were performed by RT-PCR. Results : A total of 63 patients with clinical diagnosis of enteroviral infections were enrolled; of those, 38 (60%) were positive for enterovirus. The mean age of the patients was 2 years and 7 months and the sex ratio of male to female was 0.9 :1. Their clincal manifestations included aseptic meningitis (21 cases, 55%), HFMD (16 cases, 42%), herpangina (5 cases, 13%), neonatal fever (2 cases, 5%), encephalitis (1 case, 3%), and myocarditis (1 case, 3%). Serotypes of isolated enteroviruses were EV71 (8 cases, 21%), coxsackievirus B1 (8 cases, 21%), coxsackievirus A16 (2 cases, 6%), coxsakievirus A2 (1 case, 3%), coxsakievirus A5 (1 case, 3%), and echovirus 9 (1 case, 3%). Clinical symptoms of EV71 infection included HFMD (5 cases, 63%), aseptic meningitis (3 cases, 38%), encephalitis (1 case, 13%), and myocarditis (1 case, 13%). A positive rate of C-reactive protein in EV71 was higher than those in other enterviral infections. However, there was no statistically significant difference in other laboratory findings. Conclusion : We reported on identified enteroviruses, including EV71, during a period of 3 months in the summer of 2009. In this study, EV71 infection frequently occurred in male and clinical manifestation caused by EV71 was a more severe disease than that due to other enterviral infections. There is a need for continuous surveillance of enteroviral infection and its clinical manifestations for diagnosis and treatment of enteroviral infection.

• Clinical and Experimental Pediatrics
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• v.46 no.1
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• pp.24-32
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• 2003

Purpose : Nosocomial infection with Staphylococcus aureus, especially methicillin resistant S. aureus, has become a serious concern in the neonatal intensive care unit. The aim of this study is to investigate the virulence factors, and the relationship between the antibiotic resistance and the associated genes of Staphylococcus aureus isolated from nasal cavity of neonates. Methods : Fifty one isolates of S. aureus were obtained from nasal swab taken in 28 neonates in the NICU and nursery of Pusan National University Hospital between February and May, 2001. They were tested in regard to antibiotic susceptibility, coagulase test and typing, plasmid DNA profile, as well as reactivity to enterotoxin A-E(sea, seb, sec, sed, see) genes and toxic shock syndrome toxin-1(tst) gene by polymerase chain reaction(PCR). Associated genes such as mecA, mecR1, mecI, and femA were also determined by PCR. The origin of MRSA strains was assessed using DNA fingerprinting by arbitrarily-primed polymerase chain reaction(AP-PCR). Results : Twenty three(45.1%) and six(11.8%) isolates were resistant to oxacillin and vancomycin respectively. Multidrug resistance to three or more of the antibiotics tested was observed in 51.0% of the isolates. Forty two isolates were coagulase positive and twenty two isolates had mecA gene. Sixteen isolates had both mecA and femA genes and had type I-III plasmids. 64.7% of isolates carried sec gene, and 80.4% carried tst gene. DNA fingerprinting by AP-PCR for 12 MRSA strains showed 10 distinct patterns, suggesting different origins. Conclusion : We confirmed that the prevalence of nasal carriage of S. aureus and the incidence of antimicrobial-resistant S. aureus, especially vancomycin resistance, is very high in neonates who were admitted in NICU and nursery. It is possible that these pathogens are responsible for serious nosocomial infections in neonates. The need for improved surveillance and continuous control of pathogens is emphasized.

• Journal of radiological science and technology
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• v.28 no.4
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• pp.279-286
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• 2005

Purpose : There are two modalities, those are small bowel series(SBS) and abdominal pelvic computed tomography(CT), for diagnosis of small bowel disease. The aim of this study is to lend radiological technologists who are doing the two modalities assistance in the understanding characteristic of disease by comparing the two results. Meterials and method : 284 patients were examined the two SBS and abdominal pelvic CT together from 1999 to 2003. 250 ml $BaSO_4$ suspension 40 w/v% and 600ml carboxy methyl cellulose 0.5 w/v% were used for SBS. Abdominal Pelvic CT was examined in one hour before taking 450 ml $BaSO_4$ suspension 1.5 w/v%. The CT scan was done in 72 sec after 150 ml contrast media injection. the used protocol was helical mode 5:5 mm pitch 1.375:1, speed 27.50, exposure 120 kv, 240 mA, tube rotation time 0.5 sec. the statistic analysis was conducted with statistical program SPSS 10 version with frequency and crossing analysis. P-value less than 0.05 were considered significant. Results : In the results of SBS, normal findings were 131 patients(46.1%), inflammatory bowel disease(IBD) 64(22.9%), ischemia+ileocolitis+vasculitis 22(7.7%), Obstruction+stricture 21(7.7%) and Others 45(15.9%). In the results of abdominal pelvic CT, normal findings were 103 patients(36.3%), inflammatory bowel disease 65(22.9%), wall thickening+lymphadenopathy 42(14.8%), Fluid collection 17(6%), and Others 57case(20%). The same results of the two were 130patients(45.8%). 30patients(10.6%) of normal finding in SBS were diagnosed as wall thickening+lymphadenopathy and IBD in CT, and 15patients(5.3%) of normal finding in CT were diagnosed as ischemia+ileocolitis+vasculitis, mass and IBD in SBS(p<0.05). Transit time delay was diagnosed in 10patients(3.5%) on only SBS, wall thickening+lymphadenopathy was diagnosed in 20patients(7%) in only CT(p<0.05). Conclusion : We think that proper examination method will be selected in the small bowel disease, if we understand the characteristics of the disease and method.

• Korean Journal of Organic Agriculture
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• v.27 no.4
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• pp.479-499
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• 2019

This study was conducted to investigate the responses of soil properties and microbial communities to different agricultural management and soil types, including organic management in Andisols (Org-A), organic management in Non-andisols (Org-NA), conventional management in Andisols (Con-A) and conventional management in Non-andisols (Con-NA) by using a pyrosequencing approach of 16S rRNA gene amplicon in Radish farms of volcanic ash soil in Jeju island. The results showed that agricultural management systems had a little influence on the soil chemical properties but had significant influence on microbial communities. In addition, soil types had significant influences on both the soil chemical properties and microbial communities. Organic farming increased the microbial density of bacteria and biomass C compared to conventional farming, regardless of soil types. Additionally, Org-NA had the highest dehydrogenase activity among treatments, whereas no difference was found between Org-A, Con-A and Con-NA and had the highest species richness (Chao 1) and diversity (Phyrogenetic diversity). Particularly, Chao 1 and Phyrogenetic diversity were increased in organic plots by 12% and 20%, compared with conventional plots, respectively. Also, regardless of agricultural management and soil types, Proteobacteria was the most abundant bacterial phylum, accounting for 21.9-25.9% of the bacterial 16S rRNAs. The relative abundance of putative copiotroph such as Firmicutes was highest in Org-NA plot by 21.0%, as follows Con-NA (13.1%), Con-A (6.7%) and Org-A (5.1%.), respectively and those of putative oligotrophs such as Acidobacteria and Planctomycetes were higher in Con-A than those in the other plots. Furthermore, LEfSe indicated that organic system enhanced the abundance of Fumicutes, while conventional system increased the abundance of Acidobacteria, especially in Non-andisols. Correlation analysis showed that total organic carbon (TOC) and nutrient levels (e.g. available P and exchangeable K) were significantly correlated to the structure of the microbial community and microbial activity. Overall, our results showed that the continuous organic farming systems without chemical materials, as well as the soil types made by long-term environmental factors might influence on soil properties and increase microbial abundances and diversity.

• Economic and Environmental Geology
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• v.41 no.2
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• pp.211-217
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• 2008

The gravity anomalies that observed by ground and shipborne survey and calculated from GRACE satellite are combined by using spherical cap harmonic analysis (SCHA). In this study, ground gravity data from Korea Institute of Geoscience and Mineral Resource(KIGAM) and shipborne gravity data from National Ocean Research Institute(NORI) and Korea Ocean Research and Development institute(KORDI) were used. L-2 level GRACE Gravity Model (GGM02C) was also used for satellite gravity anomaly. The ground and shipborne surveyed data were combined and gridded using Krigging method with 0.05 degree interval and GRACE data were also gridded using the same method with 0.05 degree to harmonize with the resolution of SCHA that has coefficient up to 80. Generalized Minimal Residual(GMRES) inversion method was implemented for calculating the coefficients of SCHA using the gridded ground and satellite gravity anomalies that had 0 km and 50 km altitude, respectively. The results of inversion method showed good correlation of 0.950 and 0.995 with original ground and satellite data. The gravity anomaly using SCHA satisfies Laplace's equation, therefore, using these SCHA coefficients, gravity anomaly can be calculated at any altitude. In this study, gravity anomaly was calculated from 10 km to 60 km altitude and each altitude, very stable results were shown. The ground and shipborne gravity data that have higher resolution and satellite data in long wavelength are harmonized well with SCHA coefficients and successfully applied in South Korea area. If more continuous survey and muti-altitude surveyed data like airborne data available, more precise gravity anomaly can be acquired using SCHA method.

• Korean Journal of Ecology and Environment
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• v.39 no.4 s.118
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• pp.462-470
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• 2006

The zooplankton community dynamics and grazing experiments was evaluated along a 40 km section of the lower Seomjin river system. Zooplankton was sampled twice a month from January 2005 to June 2006 at three sites (River mouth; RKO, Seomjin bridge: RK12 and Gurae bridge: RK36) in the main river channel. During the study period, the values of most limnological parameters in the three sites were fairly similar, except for conductivity. Annual variation of conductivity in River mouth and Seomjin bridge was more dramatic than which of the other site. There were statistically significant spatial and seasonal differences in zooplankton abundance (ANOVA, P<0.01). Total abundance of major zooplankton groups at both stations was much higher than in Gurae bridge. Among the macrozooplankton, cladocerans abundance was negligible in study sites during study periods. Community filtering rates (CFRs) for phytoplankton and bacteria varied from 0 to 50 mL $L^{-1}\;D^{-1}$ and from 0 to 45 mL $L^{-1}\;D^{-1}$, respectively. The spatial variation of CFRs for phytoplankton was significant (ANOVA, P<0.05). The CFRs of copepods for phytoplankton and bacteria was much higher than that of cladocerans at study sites. Total zooplankton filtering rates on bacteria were slightly lower than filtering rates on phytoplankton. The CFRs of microzooplankton (MICZ) for bacteria were much higher than for macrozooplankton (MACZ) at all sites. Considering the total zooplankton community, MICZ generally were more important than MACZ as grazers of bacteria and phytoplankton in freshwater zone, while MACZ were more important than MICZ as grazers of phytoplankton in brackish zone.

• Korean Journal of Ecology and Environment
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• v.44 no.2
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• pp.239-251
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• 2011

To compare the different water environments in the Jigsaw stream (st. 1, 2) and Jujin stream (st. 3, 4) crossing over the Byeonsan-Bando and Seonunsan parklands, the physico-chemical factors and the species compositions of epilithic diatoms were analyzed from March 2009 to February 2010. The mean values of conductivity (681.1 ${\mu}S\;cm^{-1}$), suspended solids (27.83 mg $L^{-1}$), biological oxygen demand (3.15 mg $L^{-1}$), total nitrogen (3.24 mg $L^{-1}$), total phosphorus (0.24 mg $L^{-1}$) and chlorophyll-${\alpha}$ (12.99 mg $m^{-3}$ ) in Jujin stream were significantly higher than each element in Jigsaw stream. Eighty taxa were classified into 73 species and seven varieties belonging to 24 genera, eight families, three suborders and two orders in Jigsaw stream. Eighty eight taxa were classified into 81 species and seven varieties belonging to 22 genera, seven families, three suborders and two orders in Jujin stream. Biological water quality assessments revealed that Jigsaw stream was ${\beta}$~${\alpha}$-oligosaprobic and Jujin stream was ${\beta}$-mesosaprobic. Dominant and diversity indices were indicated from 0.25~0.81 and from 1.73~4.14 in Jigsaw stream, respectively, and from 0.29~0.64 and from 2.72~4.02 in Jujin stream, respectively. Jujin stream was more eutrophic than those of Jigsaw stream. The different water environments between the two streams could be due to the different ecosystems of the neighboring basins of each stream crossing over in the mountain. Further studies should investigate the water environments of two streams by continuous and regular monitoring to analyze the different ecosystem mechanisms.