• 동의생리병리학회지
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• 제31권6호
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• pp.356-361
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• 2017

Houttuyniae Herba is widely used as a cosmetic for enhancing hair growth, and study on promoting mouse hair growth has also been reported. However, studies on the effects of the Houttuyniae Herba on dermal papilla (DP) cells, which play an important role in hair growth, are not well known. For this reason, we studied the effect of Houttuyniae Herba on DP cells. The strong antioxidant activity of Houttuyniae Herba was confirmed by ABTS assay. In the MTS assay, cell viability was reduced to 94.5% in DP cells by treatment of 2 mg/ml concentration of Houttuyniae Herb and cytotoxicity was not observed at 1 mg/ml concentration. The mRNA expression levels of Bone morphogenetic pretein (BMP6), fibroblast growth factor 7 (FGF7), FGF10, and ${\beta}$-galactosidase genes, which are involved in hair growth cycle and hair loss induction, were measured by quantitative RT-PCR after Houttuyniae Herbtreatment. Houttuyniae Herb did not significantly affect mRNA expression of BMP6, FGF7, FGF10, and ${\beta}$-catenin, which are important factors for regulating the hair cycle, including type 1 $5{\alpha}$-reductase. However, mRNA expression of type 2 $5{\alpha}$-reductase, the major cause of male hair loss, was significantly reduced to 56.1% by treatment of Houttuyniae Herbtreatment. Taken together, these results suggest that the Houttuyniae Herbtreatment can help to treat lair loss through removing free radicals and suppression of the expression level of type 2 $5{\alpha}$-reductase in DP cells.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2018년도 추계학술대회
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• pp.31-31
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• 2018

Benign prostatic hyperplasia (BPH), which is the most common disorder in elderly men, involves androgenic hormone imbalance with chronic inflammation that causes imbalance between cell apoptosis and cell proliferation. As the root cause of the BPH remains unclear and synthetic drugs for treatment of BPH have undesirable side effects, the development of effective alternative medicines has been considered. Chinese Skullcap has been considered natural remedy to treat pyrexia, micturition disorder and inflammation. Although skullcap has effective properties on various diseases, the effects and molecular mechanism of Skullcap on BPH are not fully understood. Therefore, in this study, we evaluated the efficacy of Chinese Skullcap root extract (SRE) in testosterone-induced BPH rats. Compared with the untreated group, the SRE treatment group suppressed pathological alterations, such as prostate growth and increase in serum dihydrotestosterone and $5{\alpha}$-reductase levels. Furthermore, SRE significantly decreased the expression of androgen receptor and proliferating cell nuclear antigen. SRE also restored Bax/Bcl-2 balance. These effect of SRE was more prevalent than commercial $5{\alpha}$-reductase inhibitor, finasteride. Taken together, we propose that SRE suppresses abnormal androgen events in prostate tissue and inhibits the development of BPH by targeting inflammation- and apoptosis-related markers. These finding strengthens that SRE could be used as plant-based $5{\alpha}$-reductase inhibitory alternative.

• Journal of Microbiology and Biotechnology
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• 제25권3호
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• pp.407-412
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• 2015

We investigated the effect of ultra-high molecular weight poly-γ-glutamic acid (UHMW γ-PGA) on hair loss in vitro and in vivo. 5-Alpha reductase is an enzyme that metabolizes the male hormone testosterone into dihydrotestosterone. By performing an in vitro experiment to analyze the inhibitory effects of UHMW γ-PGA on 5-alpha reductase activity, we determined that UHMW γ-PGA did in fact inhibit 5-alpha reductase activity, indicating the use of UHMW γ-PGA as a potential 5-alpha reductase inhibitor in the treatment of men with androgenetic alopecia. To evaluate the promotion of hair growth in vivo, we topically applied UHMW γ-PGA and minoxidil on the shaved dorsal skin of telogenic C57BL/6 mice for 4 weeks. At 4 weeks, the groups treated with UHMW γ-PGA showed hair growth on more than 50% of the shaved skin, whereas the control group showed less hair growth. To investigate the progression of hair follicles in the hair cycle, hematoxylin and eosin staining was performed. Histological observations revealed that the appearance of hair follicles was earlier in the UHMW γ-PGA-treated group than in the control group. The number of hair follicles on the relative area of shaved skin in the UHMW γ-PGA-treated group was higher than that observed on the shaved skin in the control group. These results indicate that UHMW γ-PGA can promote hair growth by effectively inducing the anagen phase in telogenic C57BL/6 mice.

• 대한화장품학회지
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• 제42권3호
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• pp.203-209
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• 2016

최근 남성성탈모증에 대한 관심이 증대되고 있으며, 이에 천연물 및 그의 활성성분을 활용한 새로운 약물 개발에 대한 연구가 증가하고 있다. 송지(Resina Pini, RP)는 Pinus sp. (Pinaceae)의 수지질로 전통의학적으로 감염, 우식증, 치주질환에 사용되어왔다. 본 연구진은 RP의 성분인 아비에트산(abietic acid, AA)이 남성성탈모기전에 중요한 효소인 $5{\alpha}$-reductase를 억제하는 효과를 세포 수준에서 입증한바 있으며, 이번 연구에서는 실제로 탈모억제 및 모발 성장에 대하여 실험동물 수준에서 입증하고자 한다. C3H/HeN 탈모마우스 모델에서 RP는 300 mg/kg에서 유의하게 탈모억제를 확인하였으며, 뿐만 아니라 AA는 30 mg/kg에서도 유의하게 탈모억제효과를 보였다. 이상의 결과로부터 RP는 그 활성성분인 AA가 $5{\alpha}$-reductase 억제하는 기전을 통해 남성성탈모억제효과를 보였다고 사료되며, 향후 탈모억제 보완치료법으로의 이용 가능성을 보였다.

• 대한화장품학회지
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• 제31권3호
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• pp.273-277
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• 2005

본 연구에서는 피지분비 억제 효과를 지닌 신규 화장품 원료를 대한민국 유래의 천연 식물에서 개발하고자 하였다. 다양한 식물 추출물의 5alpha-reductase에 대한 저해효과를 자외선 분광광도계를 이용한 효소 동력학적 분석법을 통하여 평가하였다. 효소 동력학적 분석을 위하여 각각 쥐의 간 조직과 배양된 인체 유래 피지선 세포에서 5alpha-reductase를 추출하였다. 그 결과, 5-AR에 대하여 뛰어난 저해효능을 보이는 3종의 식물 추출물을 선정하였고, 이들의 피지분비 억제 효과를 배양된 인체 유래 피지선 세포를 이용하여 분석하였다. 연구의 결과, 수십 종의 식물 추출물 중 해송 추출물이 가장 강력한 5-AR 저해능을 나타내었으며, 인체 유래 피지선 세포에 대하여 0.005% 농도로 처리한 경우 48%의 피지 생성량 감소 효과를 나타내었다. 해송 추출물은 피지 분비를 감소시켜 여드름이나 지루성 피부염과 같은 피부 질환에 효과를 지닌 화장품의 원료로 사용될 수 있을 것이다.

• Journal of Applied Biological Chemistry
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• 제49권1호
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• pp.24-27
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• 2006

Four ursane-type triterpenoids, 2${\alpha}$,3${\alpha}$,19${\alpha}$-trihydroxy-24-norurs-4(23),12-dien-28-oic acid (1), 4(R),23-epoxy-2${\alpha}$,3${\alpha}$,19${\alpha}$-trihydroxy-24-norurs-12-en-28-oic acid (2), myrianthic acid (3), and tormentic acid (4), and a phenolic compound, ethyl gallate (5), from an EtOAc-soluble extract of the stems of Rumex japonicus, were subjected to in vitro bioassays to evaluate advanced glycation end products (AGEs) and rat lens aldose reductase (RLAR) inhibitory activity. Compounds 1 and 5 exhibited a significant inhibitory activity on AGEs formation with $IC_{50}$ value of 87 ${\mu}M$ and on RLAR with $IC_{50}$ value of 14.3 ${\mu}M$, respectively. Ethyl gallate (5) was isolated for the first time from this plant.

• 한방안이비인후피부과학회지
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• 제15권1호
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• pp.315-335
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• 2002

This study was carried out to prove the effects of CBTLC on the $5{\alpha}$-reductase inhibition, the sterilizing power for Propionibactrium acnes, the cytotoxicity of human monocyte, the inhibition for prosta-glandins($PGE_2$), Interleukins(IL-$1{\beta}$) and TNF-${\alpha}$ in inflammation, and the size of Hamster ear sebaceous gland concerned with Acnes. The result were obtained as follows : 1. On the $5{\alpha}$-reductase inhibition of CBTLC in vitro, An undiluted solution of CBTLC was $71{\%}$ inhibition on $5{\alpha}$-reductase and $\frac{1}{10}$ diluted solution of CBTLC was $20{\%}$ inhibition on $5{\alpha}$-reductase. 2. On the sterilizing power for Propionibactrium acnes concerned in Acnes, an undiluted solution and $\frac{1}{10}$ diluted solution of CBTLC formed 12mm clear zone diameters. 3. CBTLC was showed the cytotoxicity of human monocyte in $0.08{\%}$ and $0.12{\%}$ of CBTLC. 4. $0.01{\%}$ and $0.04{\%}$ of CBTLC inhibited the production of prostaglandins($PGE_2$) in the human monocyte stimulated with P. acnes LPS. 5. $0.08{\%}$ and downward of GMHBS inhibited the production of interleukins(lL-$1{\beta}$) in the human monocyte stimulated with P. acnes LPS. 6. $0.08{\%}$ and $0.12{\%}$ of GMHBS inhihited the production of TNF-${\alpha}$ in the human monocyte stimulated with P. acnes LPS. 7. As the antiandrogenic compound, CBTLC was used in hamster ears with topical application. CBTLC was diminished slightly on the size of hamster ears sebaceous gland.

• 한국약용작물학회지
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• 제18권5호
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• pp.305-310
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• 2010

Taxifolin-3-O-$\beta$-D-xylopyranoside and quercetin-3-O-$\alpha$-L-rhamnopyranoside were isolated from an EtOAc-soluble extract of the leaves of Chamaecyparis obtuse. Quercetin-3-O-$\alpha$-L-rhamnopyranoside was found to possess a potent inhibitory activity of human recombinant aldose reductase in vitro, its $IC_{50}$ value being $11.5\;{\mu}M$. Kinetic analysis showed that quercetin-3-O-$\alpha$-L-rhamnopyranoside exhibited uncompetitive inhibition against DL-glyceraldehyde. Also, quercetin-3-O-$\alpha$-L-rhamnopyranoside suppresses sorbitol accumulation in rat lens under high glucose conditions, demonstrating the potential to prevent sorbitol accumulation in vivo. These results suggest that this compound may be a promising agent in the prevention or treatment of diabetic complications.

• 대한한의학회지
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• 제26권1호
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• pp.134-147
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• 2005

Objective: This study was carried out to investigate the effects of Gami-Shengmayuipung-tang on acne. Methods : The effects of Gami-Shengmayuipung-tang (SYTSRG) on acne were measured by the $5\alpha-reductase$ inhibition, the sterilizing power for Propionibactrium acnes, the cytotoxicity of human monocyte, the inhibition for prostaglandins$(PGE_2)$, interleukins $(IL-l\beta)$ and $TNF-\alpha$ in inflammation, and the size of the hamtster ear sebaceous gland related to P. acnes. Results: On the $5\alpha-reductase$ inhibition of SYTSRG in vitro, an undiluted solution of SYTSRG showed $80\%$ inhibition on $5\alpha-reductase$ and 1/10 diluted solution of SYTSRG showed $40\%$ inhibition on $5\alpha-reductase$. On the sterilizing power for Propionibactrium acnes related acne, an undiluted solution and 1/10 diluted solution of SYTSRG formed $12\beta{\AE}$ clear zone diameters. SYTSRG did not show cytotoxicity of human monocyte. Concentrations of $0.01\%\;and\;0.04\%\;and\;0.08\%$ of SYTSRG inhibited the production of prostaglandins $(PGE_2)$ in the human monocyte stimulated with P. acnes LPS. $0.08\%$ and less of SYTSRG inhibited the production of interleukins $(IL-l\beta)$ in the human monocyte stimulated with P. acnes LPS. Concentrations of $0.04\%,\;0.08\%\;and\;0.12\%$ of SYTSRG inhibited the production of $TNF-\alpha$ in the human monocyte stimulated with P. acnes LPS. As the antiandrogenic compound, SYTSRG was used in hamster ears with topical application. SYTSRG diminished the size of the hamster ear sebaceous gland in males, but not in females. Conclusion: The present data suggest that SYTSRG may affect the primary stage of inflammation of acne.

• Mass Spectrometry Letters
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• 제3권1호
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• pp.21-24
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• 2012

$5{\alpha}$-Dihydrotestosterone (DHT) is the primary active metabolite of testosterone, catalyzed by $5{\alpha}$-reductase ($5{\alpha}R$) in the skin, prostate, and liver. In this study, the $5{\alpha}R$ activity in rat liver S9 fraction in the presence of a NADPH-generating system was evaluated and compared by gas chromatography-mass spectrometry (GC-MS)-based in vitro assays. Testosterone and a $5{\alpha}R$ inhibitor, finasteride, were added to the S9 fractions and incubated at $37^{\circ}C$ for 1 h. Both testosterone and DHT were quantitatively measured and compared with two different GC-MS-based steroid profiling techniques. DHT was not detected by conventional GC-MS analysis in the absence of finasteride when the concentration of testosterone in the S9 fraction was less than $0.2{\mu}M$, whereas the isotope-dilution GC-MS (GC-IDMS) system was able to evaluate the $5{\alpha}R$ activity. Because the S9 fraction contains more reactive enzymes and is easier to collect from tissues compared with a microsomal solution, the combination of the S9 fraction and GC-IDMS technique may be a promising assay for evaluating the $5{\alpha}R$ activity in large-scale clinical studies.