• Biotechnology and Bioprocess Engineering:BBE
• /
• 제9권4호
• /
• pp.278-284
• /
• 2004

Leuconostoc citreum is one of the representative strains of Leuconostoc spp. that show fast growth rates in fermented vegetables. Sequential experimental designs including the Plackett-Burman design, fractional factorial design, steepest ascent analysis, central composite design and response surface methodology were introduced to optimize and improve the medium for L. citreum. Fifteen medium ingredients were examined and glucose ($20 g/\ell$), yeast extract ($12.5g/\ell$), sodium acetate trihydrate ($6.12g/\ell$), potassium phosphate ($42.55g/\ell$), and dibasic ammonium citrate ($4.12g/\ell$), were chosen as the best components to give a critical and positive effect for cell-growth. The biomass was increased to ($2.79g/\ell$), (169%), compared to the $1.65g/\ell$ in MRS medium.

• 동력기계공학회지
• /
• 제21권5호
• /
• pp.86-91
• /
• 2017

Friction Stir Welding (FSW) is useful technique to join aluminum alloy with energy efficient and environment friendly. In this paper, the design of experiment with three-way factorial design was adopted for optimum conditions of welding variables in the FSW of Al 6061 alloy. Tools of shoulder diameter of 9, 12, 15 mm and pin length of 1.5 mm were used. Also the material's dimension for welding were $2{\times}100{\times}150mm$, and the tensile specimens were worked by water-jet technique. Welding variables were shoulder diameter, rotating speed and travel speed of tool. From the results of this work, the welding factor influenced on yield strength most was travel speed and the optimum condition for FSW was predicted as the shoulder diameter of 15 mm, welding speed of 500 mm/min and rotating speed of 2,000 rpm. Also the presumption range of yield strength at optimal condition of reliability 99% was estimated to $207.19{\pm}9.91MPa$.

• Journal of Applied Biological Chemistry
• /
• 제58권3호
• /
• pp.209-218
• /
• 2015

Fructooligosaccharides have been mainly produced by microbial fructosyltransferases (FTase) enzymes. The present work focuses on the optimization of medium composition and cultivation parameters affecting FTase produced by Penicillium aurantiogriseum AUMC 5605 in shake flask cultivation. FTase production was optimized in two steps using DeMeo's fractional factorial design. A 1.46-fold increase in FTase production (105.4 U/mL) was achieved using the optimized culture medium consisting of (g/L): sucrose, 600; yeast extract, 10; $K_2HPO_4$, 5; $MgSO_4{\cdot}7H_2O$, 0.5; $(NH_4)_2SO_4$, 1.0 and KCl, 0.5. The obtained results showed that the maximum FTase enzyme activity was produced at initial cultivation pH values ranging from 6.0-6.5, at agitation speed of 200 rpm and using vegetative fungal cells as inoculum. Moreover, results showed that optimization of medium composition and some cultivation parameters resulted in an increase of about 93.7% in the enzyme activity than the nonoptimized cultivation conditions after 96 h of cultivation. Additionally, maximum production and specific production rates recorded 2340 U/L/h and 102 U/L/h/g cells, respectively.

• Advances in Energy Research
• /
• 제4권1호
• /
• pp.69-86
• /
• 2016

The quest to reduce the level of overdependence on fossil fuel product and to provide all required information on proven existing alternatives for renewable energy has resulted into rapid growth of research globally to identify efficient alternative renewable energy sources and the process technologies that are sustainable and environmentally friendly. The present study is aimed at production and characterization of bioethanol produced from sugarcane juice using a $2^4$ factorial design investigating the effect of four parameters (reaction temperature, time, concentration of bacteria used and amount of substrate). The optimum bioethanol yield of 19.3% was achieved at a reaction temperature of $30^{\circ}C$, time of 72 hours, yeast concentration of 2 g and 300 g concentration of substrate (sugarcane juice). The result of statistical analysis of variance shows that the concentration of yeast had the highest effect of 7.325 and % contribution of 82.72% while the substrate concentration had the lowest effect and % contribution of -0.25 and 0.096% respectively. The bioethanol produced was then characterized for some fuel properties such as flash point, specific gravity, cloud point, pour point, sulphur content, acidity, density and kinematic viscosity. The results of bioethanol characterization conform to American society for testing and materials (ASTM) standard. Hence, sugarcane juice is a good and sustainable feedstock for bioethanol production in Nigeria owing relative abundance, cheap source of supply and available land for large scale production.

• Journal of Microbiology and Biotechnology
• /
• 제28권6호
• /
• pp.902-908
• /
• 2018

Optimization of the culture medium to maximize menaquinone-7 (MK-7) production by Bacillus subtilis strain KCTC 12392BP in static culture was carried out using statistical experimental methods, including one factor at a time, fractional factorial design, and response surface methodology (RSM). Maltose (carbon source), tryptone (nitrogen source), and glycerol (activator) were identified as the key medium components for MK-7 synthesis by the fractional factorial design, and were selected for statistical optimization by RSM. The statistical analysis indicated that, in the range that was studied, maltose, tryptone, and glycerol were all critical factors having profound effects on the production of MK-7, with their coefficients for linear and quadratic all significant at the p < 0.05 level. The established model was efficient and feasible, with a determination coefficient ($R^2$) of 0.9419. The predicted concentrations of maltose, tryptone, and glycerol in the optimal medium were determined as 36.78, 62.76, and 58.90 g/l, respectively. In this optimized medium, the maximum yield of MK-7 reached a remarkably high level of $71.95{\pm}1.00{\mu}g/ml$ after 9 days of static fermentation, which further verified the practicability of this optimized strategy.

• 복식
• /
• 제59권6호
• /
• pp.41-57
• /
• 2009

Evaluations of fashion products are often influenced by consumers' knowledge of the country where the products were made in. As globalization progresses, country-of-origin information is widely regarded as a powerful cue on consumers' shopping behavior. The purpose of study was to examine the consumer evaluation of apparel products with uni-national or bi-national country of origin. The effect of price level and gender were also investigated. The empirical research design took 2${\times}$2 factorial design with the country-of-origin coincidence (uni-national vs. bi-national) and price (high vs. low) of gender (male vs. female). Consumers' ethnocentrism and country-of-origin interest were taken into account as covariates in the factorial design. The consumers' evaluation of fashion products was measured in terms of brand attitudes and product attitudes. Data from 514 respondents were analyzed with t-test, one-way ANOVA, two-way ANOVA, and ANCOVA. Results of this study affirmed the importance of price information rather than country-of-origin coincidence in brand attitudes and product attitudes. Only for male consumers, interaction effects of price and country-of-origin coincidence had significant eflects on utilitarian attitude. Effects of two covariate variables included in the study were significant for female respondents but not for male respondents.

• 한국정밀공학회:학술대회논문집
• /
• 한국정밀공학회 2012년도 춘계학술대회 논문집
• /
• pp.443-444
• /
• 2012

• 한국연초학회지
• /
• 제20권1호
• /
• pp.131-138
• /
• 1998

In order to minimize the trial frequency in the new filter cigarette design, we studied the relationship between smoke yield and tipping materials of cigarette. A three levels full factorial design involving filament denier (X1,2.5-3.3d), Porosity of the acetate filter plug wrap (X2, 3,500-16,000CU) and porosity of the tip paper (X3, 400-1,200CU) was used. Three independent factors (Xl, X2, X3) were chosen for their effects on the various responses and the function was expressed in terms of a quadratic polynomial equation, Y : $\beta$o + $\beta$1Xl + $\beta$2X2 + $\beta$3X3 + $\beta$11Xl2 + $\beta$22X22+ $\beta$33X32 + $\beta$12X1X2 + $\beta$13XIX3 $\beta$23X2X3 which measures the linear, quadratic, and interaction effects. Twenty-nine trial numbers were obtained as a results of using a three levels full factorial design and it was analyzed by the multiple regression analysis with backward stepwise in STATISTICA/pc under restricted conditions. Tar yields of the cigarette was affected by porosity of tip paper (0.66), filament denier (0.47) and porosity of plug wrap (0.28) in the decreasing order, and linear effect of tip paper porosity (B3) and filament denier (91) were significant at a level of 0.01($\alpha$). The filament denier and tipping paper porosity interaction F ratio among three factors had a P-value of 0,000041, indicating higher interaction between these factors. Based on the analysis of variance, the model fitted for Tar (Y1) was significant at 5% confidence level and the coefficient of determination (0.96) was the proportion of variability in the data fitted for by the model.

• International Journal of Korean Welding Society
• /
• 제1권2호
• /
• pp.36-44
• /
• 2001

To get the appropriate welding process variables, mathematical modeling in conjunction with many experiments is necessary to predict the magnitude of weld bead shape. Even though the experimental results are reliable, it has a difficulty in accurately predicting welding process variables for the desired weld bead shape because of nonlinear and complex characteristics of welding processes. The welding condition determined for the desired weld bead shape may cause the weld defect if the welding current/voltage/speed combination is improperly selected. In this study, the $2^{n-1}$ fractional factorial design method and correlation parameter were used to investigate the effect of the welding process variables on the fillet joint shape, and the multiple non-linear regression analysis was used for modeling the gas metal arc welding(GMAW）parameters of the fillet joint. Finally, a fuzzy rule-based method and a neural network method were proposed so that the complexity and non-linearity of arc welding phenomena could be effectively overcome. The performance of the proposed neuro-fuzzy system was evaluated through various experiments. The experimental results showed that the proposed neuro-fuzzy system could effectively check the welding conditions as to whether or not weld defects would occur, and also adjust the welding conditions to avoid these weld defects.

• 한국생산제조학회지
• /
• 제20권1호
• /
• pp.28-33
• /
• 2011

In this paper, structural design for 2kW class composite blade is performed by using design of experiment(DOE). A full factorial design is applied to meet the design specifications at the manufacturing process. The analysis of variance(ANOVA) is made in order to determine the significance of effects in an analysis. Structural analysis by using of commercial software ABAQUS is performed to compute the displacement and safety factor of filament wound composite blade. The results show that the proposed method is suitable to analyze the factors at the design of wind turbine blade.