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http://dx.doi.org/10.3839/jabc.2015.033

Optimization of Medium Composition and Cultivation Parameters for Fructosyltransferase Production by Penicillium aurantiogriseum AUMC 5605  

Farid, Mohamed Abdel-Fattah Mohamed (Natural and Microbial Products Department, National Research Centre)
Kamel, Zinat (Faculty of Science, Cairo University)
Elsayed, Elsayed Ahmed (Natural and Microbial Products Department, National Research Centre, Advanced Chair for Proteomics and Cytomics Research, Faculty of Science, King Saud University)
El-Deen, Azza Mohamed Noor (Natural and Microbial Products Department, National Research Centre)
Publication Information
Journal of Applied Biological Chemistry / v.58, no.3, 2015 , pp. 209-218 More about this Journal
Abstract
Fructooligosaccharides have been mainly produced by microbial fructosyltransferases (FTase) enzymes. The present work focuses on the optimization of medium composition and cultivation parameters affecting FTase produced by Penicillium aurantiogriseum AUMC 5605 in shake flask cultivation. FTase production was optimized in two steps using DeMeo's fractional factorial design. A 1.46-fold increase in FTase production (105.4 U/mL) was achieved using the optimized culture medium consisting of (g/L): sucrose, 600; yeast extract, 10; $K_2HPO_4$, 5; $MgSO_4{\cdot}7H_2O$, 0.5; $(NH_4)_2SO_4$, 1.0 and KCl, 0.5. The obtained results showed that the maximum FTase enzyme activity was produced at initial cultivation pH values ranging from 6.0-6.5, at agitation speed of 200 rpm and using vegetative fungal cells as inoculum. Moreover, results showed that optimization of medium composition and some cultivation parameters resulted in an increase of about 93.7% in the enzyme activity than the nonoptimized cultivation conditions after 96 h of cultivation. Additionally, maximum production and specific production rates recorded 2340 U/L/h and 102 U/L/h/g cells, respectively.
Keywords
DeMeo's fractional factorial design; fructosyltransferases; growth kinetics; medium optimization; Penicillium aurantiogriseum;
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