• 제목/요약/키워드: $2^k$ factorial design

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Low-Cost Cultivation and Sporulation of Alkaliphilic Bacillus sp. Strain AK13 for Self-Healing Concrete

  • Hong, Minyoung;Kim, Wonjae;Park, Woojun
    • Journal of Microbiology and Biotechnology
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    • v.29 no.12
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    • pp.1982-1992
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    • 2019
  • The alkaliphilic, calcium carbonate precipitating Bacillus sp. strain AK13 can be utilized in concrete for self-repairing. A statistical experimental design was used to develop an economical medium for its mass cultivation and sporulation. Two types of screening experiment were first conducted to identify substrates that promote the growth of the AK13 strain: the first followed a one-factor-at-a-time factorial design and the second a two-level full factorial design. Based on these screening experiments, barley malt powder and mixed grain powder were identified as the substrates that most effectively promoted the growth of the AK13 strain from a range of 21 agricultural products and by-products. A quadratic statistical model was then constructed using a central composite design and the concentration of the two substrates was optimized. The estimated growth and sporulation of Bacillus sp. strain AK13 in the proposed medium were 3.08 ± 0.38 × 108 and 1.25 ± 0.12 × 108 CFU/ml, respectively, which meant that the proposed low-cost medium was approximately 45 times more effective than the commercial medium in terms of the number of cultivatable bacteria per unit price. The spores were then powdered via a spray-drying process to produce a spore powder with a spore count of 2.0 ± 0.7 × 109 CFU/g. The AK13 spore powder was mixed with cement paste, yeast extract, calcium lactate, and water. The yeast extract and calcium lactate generated the highest CFU/ml for AK13 at a 0.4:0.4 ratio compared to 0.4:0.25 (the original ratio of the B4 medium) and 0.4:0.8. Twenty-eight days after the spores were mixed into the mortar, the number of vegetative cells and spores of the AK13 strain had reached 106 CFU/g within the mortar. Cracks in the mortar under 0.29 mm were healed in 14 days. Calcium carbonate precipitation was observed on the crack surface. The mortar containing the spore powder was thus concluded to be effective in terms of healing micro-cracks.

A study on the application of DOE for optimization of blending oil with non-esterified biodiesel fuel at partial engine load (부분부하에서 비에스테르화 바이오디젤 5% 혼합유의 성능최적화를 위한 실험계획법 적용에 관한 연구)

  • Kim, Hee-Jung;Koh, Dae-Kwon;Yang, Ju-Ho;Koh, Sung-Wi;Kim, Yeong-Sik;Jeong, Tae-Young;Jung, Suk-Ho
    • Journal of Power System Engineering
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    • v.20 no.2
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    • pp.51-57
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    • 2016
  • Non-esterified biodiesel fuel is cheaper than esterified that because of a simple manufacturing process that only consists of filtering. Applicability of this on diesel engine with electronic control system was accomplished, then optimization adopting a fractional factorial design and response surface methodology was carried out at 25% and 50% of engine load in this study. Pressure of common rail and injection timing mainly effected on responses as specific fuel oil consumption and nitrogen oxides regardless of engine load. Estimations were 310.3 g/kWh of specific fuel oil consumption and 237 ppm of nitrogen oxides at 25% load, and 233.2 g/kWh of specific fuel oil consumption and 730 ppm of nitrogen oxides at 50% load. Tests to verify these estimations were accomplished and as the results, specific fuel oil consumption was 300.4 g/kWh and NOx was 277 ppm at 25% load and 236.8 g/kWh and 573 ppm at 50% load.

Analysis of Extruded Pectin Extraction from Apple Pomace by Response Surface Methodology

  • Shin, Hae-Hun;Kim, Chong-Tai;Cho, Yong-Jin;Hwang, Jae-Kwan
    • Food Science and Biotechnology
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    • v.14 no.1
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    • pp.28-31
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    • 2005
  • To extract apple pectins, apple pomace (AP) was extruded under 14 different conditions of screw speed (250-350 rpm), feed rate of 30-40 kg/hr, and 20-30% moisture content using twin-screw extrusion. Response surface methodology (RSM), based on three variables by three-level factorial design, was employed to investigate effects of screw speed, feed rate, and moisture on dependent variables of extrudates, soluble dietary fiber (SDF), yield of anhydrogalacturonic acid ($Y_{AGA}$) representing pectin, and intrinsic viscosity ([${\eta}$]). Second order models were used to generate three-dimensional response surface for dependent variables, and their coefficients of determination ($R^2$) ranged from 0.96 to 0.99. Moisture content showed highest effect on solubilization of AP.

Optimization of Preparation Conditions of Polymannuronate Acorn Mook Using RSM (반응 표면 분석법을 이용한 폴리만뉴로닉산 도토리묵 제조 조건의 최적화)

  • Choi, Hee-Sook;Ko, Soon-Nam;Lee, Kyoung-Hae
    • Journal of the East Asian Society of Dietary Life
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    • v.17 no.1
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    • pp.103-109
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    • 2007
  • The optimum conditions for springiness of polymannuronate acorn mook, in which polymannuronate was added to acorn powder, was investigated by the response-surface method(RSM). The fractional factorial design with three variables, i.e. polymannuronate addition, water addition and boiling time, and with three levels revealed that the range of acorn springiness was $0.804{\sim}0.987$. The governing equation was also partially differentiated for boiling time, showing that the optimum manufacturing condition for $90^{\circ}C$ is addition of 2% polymannuronate, addition of 120 mL of water, and 60 minutes of boiling time.

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The Effect of Online Supporter's Review Directions on Consumers' Brand Attitude and Purchase Intention: The Role of Brand Awareness

  • Lee, SuMin;Lee, Chunghee;Lee, MiYoung
    • Journal of Fashion Business
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    • v.20 no.6
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    • pp.135-147
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    • 2016
  • Online supporters are the group of people selected by companies for the online promotion of their products or services and focus on generating messages that are conducive to stimulating hands-on experiences with companies' products and services to create advertising effects. This study examined how reviews offered by blogs operated by fashion brands' online supporters influence consumer's brand attitudes and purchase intentions. Specifically, this study examined how brand awareness and directions of review messages influences consumers' brand attitudes and purchase intentions. This study employed a 2 (brand awareness: high awareness vs. low awareness) ${\times}$ 3 (review direction: one-sided positive, two-sided positive & negative, one-sided negative) between-subject factorial design. In total, 180 respondents participated, thus garnering 30 responses for each of the six conditions. The results of two-way ANOVA revealed the significant main effect supporters' review message direction on consumers' brand attitudes and purchase intentions. Two-sided messages were rated high for brand attitude and purchase intention compared to one-sided positive or negative or positive directions. The interactions between brand reputation and message direction were significant for brand attitude, but not for purchase intention.

Investigation of Factors on the Sensory Characteristics of Milk Bread with Tumeric Powder (Curcuma longa L.) Using Fractional Factorial Design Method (부분배치법을 활용한 울금 분말 첨가 우유식빵의 관능적 영향 인자 탐색)

  • Jung, Kyong Im;Park, Jae Ha;Kim, Mi Jeong
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.43 no.4
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    • pp.592-603
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    • 2014
  • We developed various recipes of turmeric powder (Curcuma longa L.) added to milk bread and assessed the individual effects of seven ingredients [milk ($X_1$), turmeric powder ($X_2$), bread improver ($X_3$), fresh yeast ($X_4$), butter ($X_5$), sugar ($X_6$), and salt ($X_7$)] as well as the 2-way interaction effects of the ingredients on the sensory characteristics of breads using fractional factorial design method. The center and end points of each component were determined via literature review and multiple test baking. Seven trained sensory test panels evaluated the outside appearance (OA), inside appearance (IA), and flavor & texture (FT) of 38 breads using 46 items of sensory evaluation. Findings are as follows: for the OA, $X_1$ (P<0.05) and $X_4$ (P<0.0001) exhibited significant individual effects, whereas $X_1*X_7$, $X_2*X_5$, $X_3*X_6$, and $X_4*X_6$ indicated significant interaction effects (P<0.05). For the IA, $X_1$ (P<0.0001), $X_4$ (P<0.0001), $X_6$ (P<0.05), $X_2*X_4$ (P<0.05), and $X_3*X_6$ (P<0.01) showed individual and interaction effects, respectively. For the FT, $X_1$ and $X_2$ showed the most significant individual effect (P<0.0001), followed by $X_4$, $X_5$ and $X_6$ (P<0.05) in descending order. $X_4*X_7$ indicated the only significant interaction effect. We computed the magnitudes of the 2-way interaction effects of the ingredients with a distinct emphasis. Model equations predicting the levels of the ingredient effects on the breads were also provided via regression analyses. In summation, $X_4$ appeared to be the most significant component affecting the sensory characteristics based on its individual and 2-way interaction effects. Further, $X_6$, $X_1$, $X_2$, and $X_5$ indicated both individual and interaction effects. $X_3$ and X7 showed only interaction effects. The center point effect appeared to be unequivocal for whole sensory characteristics. Findings of the present study may provide insights into the selection of ingredients to derive an optimal model for turmeric powder-added bread using the response surface method hereafter.

Performance Optimization of the Two-Stage Gas Gun Based on Experimental Result (2-단계 기포(氣砲)의 성능 최적화에 관한 연구)

  • 이진호;배기준;전권수;변영환;이재우;허철준
    • Proceedings of the Korean Society of Propulsion Engineers Conference
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    • 2003.10a
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    • pp.145-150
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    • 2003
  • The present study aims to optimize the performance of the Two-Stage Gas Gun by using the experimentally obtained data. RSM(Response Surface Method) was adopted in the optimization process to find the operating parameter than can maximize the projectile speed with the minimum number of tests. To decide the test points which results can consist of the response surface, 3$^{k}$ full factorial method was used, and the design variables were chosen with piston mass and 2$^{nd}$ driver fill pressure. The response surface was composed by nine test results and consequently the optimization was done with GENOCOP III, inherently GA code, in order to seek the optimal test point. The optimal test condition from the response surface was verified by the experiment. Results showed that the optimization process with response surface can successfully predict the test results fairly well. This study shows the possibility of performance optimization for the experimental facilities using numerical optimization algorithm.

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Persuasion Effects of Scarcity Message in Online Cosmetic Advertising: Focused on Moderating Effect of Product Type (온라인 화장품 광고의 희소성 메시지 설득효과: 제품 유형의 조절효과를 중심으로)

  • Park, Hyun-Hee
    • Journal of the Korean Home Economics Association
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    • v.48 no.10
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    • pp.121-132
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    • 2010
  • This study was intended to identify the persuasion effects of online cosmetic advertising according to scarcity message type and product type. For the experiment, 4 stimuli were developed as experimental stimuli for the 2(scarcity message type: quantity scarcity message vs distinctiveness scarcity message) X 2(product type: hedonic product vs utilitarian product) factorial design. Participants were 160 students, and they allocated 40 students in each group. They responded a questionnaire. The results were as follows. First, distinctiveness scarcity message showed more effective than quantity scarcity message in advertising attitude, product attitude, brand attitude, and purchase intention. Second, hedonic product showed more effective than utilitarian product in advertising attitude, product attitude, brand attitude, advertising attention, click-through intention, and purchase intention. Third, there was interaction effect according to scarcity message type and product type on the aspect of advertising attitude, advertisement attention, and click-through intention.

Influence of Hairstyle on Women's Professional Image

  • Lee Myoung-Hee
    • International Journal of Costume and Fashion
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    • v.4 no.2
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    • pp.56-65
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    • 2004
  • The purpose of this study was to investigate the effect of hairstyle, hair length, and hair color on women's professional image. The quasi-experimental method by a $2{\times}3{\times}3\;(hairstyle\;{\times}\;hair length\;{\times}\;hair color)$ factorial design was used. Subjects were 343 women in Seoul, Korea. The bright brown hair was perceived to be less professional than the black and the dark brown. The straight hair was evaluated to be more professional than the wavy hair in the short and the medium length hair, whereas the wavy conveyed a higher professionalism than the straight style in long hair. The shorter the hair was, the more professional the image was. Among 18 stimuli, the short straight hair in black was evaluated to have the highest in professional image, and the long straight hair in bright brown was the lowest. Perceivers in their 40's and 50's evaluated the bright brown hair to be more professional than those in their 10's to 30's did. The present findings provide that hairstyle, hair length, and hair color are significant cues when perceiving women's professional image.

Effects of Prohexadione-Ca, Ethephon, and Water Stress on Growth and Productivity of 'Golden Delicious'/M.9 Apple

  • Guak, Sunghee
    • Horticultural Science & Technology
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    • v.31 no.1
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    • pp.38-49
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    • 2013
  • Prohexadione-calcium (Pro-Ca), ethephon and transient water stress were evaluated in a factorial design, as potential inhibitors of early-season shoot growth of high density orchard management of apple (Malus domestica Borkh.) trees. In the experiment, water stress was imposed to one-half of the 7-year-old 'Golden Delicious'/M.9 apple trees in each of 5 blocks, by stopping irrigation for 3 weeks between 35 and 56 days after full bloom (AFB). Within each whole unit, the following Pro-Ca and ethephon treatments were randomly allocated at $2{\times}2$ factorial: a) 0 or 250 $mg{\cdot}L^{-1}$ a.i. Pro-Ca applied at 28 days AFB and b) 0 or 300 $mg{\cdot}L^{-1}$ a.i. ethephon applied twice (35 and 71 days AFB). All trees were hand thinned to king flowers prior to treatments. Vegetative shoot growth was markedly reduced by Pro-Ca, with its effect being obvious within 14 days after application, while ethephon and water stress treatments were less effective. Pro-Ca had no effect on fruit set and yield but slightly increased fruit size. Ethephon substantially reduced the fruit size and yield but had no effect on fruit set. Water stress reduced fruit set, fruit size and yield. With regard to fruit quality, Pro-Ca did not influence fruit shape, flesh firmness and soluble solids contents (SSC) but slightly reduced titratable acidity. Ethephon had no effect on fruit shape but increased firmness, SSC and acidity, while water stress did not influence these fruit quality attributes. Dry weight of dormant spur buds was reduced by both Pro-Ca and water stress, while increased by ethephon. The larger dormant buds led to the larger spur flowers at the tight cluster stage the following spring. Return flowering was promoted only by ethephon, especially on previous season's shoots. There were no significant interactions between Pro-Ca and ethephon or water stress on most variables observed in this study.