• Title/Summary/Keyword: ${\beta}-glucan$ content

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Physicochemical Properties of Breeding Lines of Colored Barleys (유색보리 육성계통의 이화학적 특성)

  • Choi, Jae-Seong;Park, Soo-Jin;Joung, Yong-Myeon;Kim, Jung-Gon;Won, Mi-Hee;Kang, Myung-Hwa
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.51 no.spc1
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    • pp.125-132
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    • 2006
  • The below is the results of physicochemical analysis of 40 breeding lines of colored barley (CB) whose systems are different Water content of CB No. 24 showed the lowest value of 7.4% while CB No. 9 showed the highest value of 10.8%. Crude protein of CB $9.7{\sim}12.9%$ range was found. Crude fat content of CB No. 6 showed the highest value of 4.35% while CB No. 34 showed the lowest of 1.35%. Crude ash content of CB No. 31 showed the lowest value of 1.20%. Ca content of CB No. 10 showed the highest value of 717.50 mg% while general barley showed the lowest value of 442.82 mg%. Mg content of CB No. 10 showed the highest value of 1320.00 mg%. Cu content of CB No. 20 showed the lowest value of 2.20 mg% while CB No. 33 showed the highest value of 6.25 mg%. K content of CB No. 20 showed the lowest value of 723.24 mg% while CB No. 1 showed the highest value of 1002.50 mg%. Mn content of CB No. 28 showed the lowest value of 31.72 mg% while general barley showed the highest value of 94.56 mg%. ${\beta}-Glucan$ content of CB No. 25 showed the lowest value of 5.20 mg% while CB No. 28 showed the highest value of 4.46 mg%.

Changes of Physicochemical Characteristic and Functional Components of Cereals for Saengsik, Uncooked Food by Drying Methods (건조방법에 따른 생식 원료 곡류의 이화학적 특성 및 기능성 성분의 변화)

  • Jin, Tie-Yan;Oh, Deog-Hwan;Om, Ae-Son;Lee, Heon-Ok;Eun, Jong-Bang
    • Food Science and Preservation
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    • v.12 no.5
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    • pp.442-448
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    • 2005
  • To develop efficient drying method, one of important process in manufacturing of Saengsik, uncooked food, the change of physicochemical characteristics and functional component contents of cereals dried by various drying methods was investigated. The proximate composition and dietary fiber content of rice, brown rice, glutinous rice and barley showed little changes during hot-air drying (50$\circC$, 55$\circC$, 60$\circC$) and freeze-drying. Yellowness(b) value of hot-air dried products was higher than one of freeze-dried products. The content of niacin was 0.77 mg% in milled rice, 1.84 mg% in brown rice, 0.68 mg% in glutinous rice, 1.00 mg% in barely dried by hot-air at 60$\circC$ and was lower than those of other samples. $\beta$-glucan content in barely was not changed by hot-air drying and freeze-drying method. In conclusion, hot-air drying would be recommended for barley having $\beta$-glucan, a kind of functional components and freeze-drying should be used for raw materials having heat-labile components such as niacin.

Isolation and Characterization of a Water-Soluble Polysaccharide from the Mycelia of Solid Cultured Agaricus blazei Murill

  • Chung, Ha-Yull;Cho, Young-Jun;Kim, Tae-Wook
    • Food Science and Biotechnology
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    • v.14 no.2
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    • pp.259-262
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    • 2005
  • A fraction (CMEx-AH-${\beta}$) of water-soluble polysaccharides, showing selective antitumor activities, was isolated from the mycelia of solid cultured Agaricus blazei Murill by hot-water extraction, ethanol precipitation, and series of chromatography. Chemical characteristics of CMEx-AH-${\beta}$, were as follows: carbohydrate content, 48%; monosaccharide composition, Man:Glu:Gal (2:93:5); molecular weight, $2{\times}10^5$; uronic acid content, 6.2%. Fundamental structure of CMEx-AH-${\beta}$, was deduced as ${\beta}-(1\;{\rightarrow}\;6)$-D-glucan with ${\beta}-(1\;{\rightarrow}\;3)$-D-glucosidic side chains based on results of methylation and $^{13}C$ NMR spectroscopic analyses.

Isolation and Characterization of a Water-Soluble Polysaccharide from the Mycelia of Solid Cultured Phellinus linteus

  • Chung, Ha-Yull;Kim, Tae-Wook
    • Food Science and Biotechnology
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    • v.14 no.6
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    • pp.783-787
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    • 2005
  • Fraction (PMEx-AH-${\beta}$) of water-soluble polysaccharide, showing stimulating activity against macrophages, was isolated from mycelia of solid cultured Phellinus linteus by hot-water extraction, ethanol precipitation, and chromatography. Chemical characteristics of PMEx-AH-${\beta}$ were as follows: carbohydrate content, 71%; monosaccharide composition, Man:Glu:Gal (9:64:27); molecular weight, $1-7{\times}10^4$; uronic acid content, 6.8%. Fundamental structure of PMEx-AH-${\beta}$ is deduced as ${\beta}$-($1{$\rightarrow}6$)-D-glucan with ${\beta}$-($1{\rightarow}3$)-D-glucosidic side chains based on methylation analysis.

Characterization of $\beta$-1,4-D-Glucan Glucanohydrolase Purified from Trichoderma koningii (Trichoderma koningii에서 분리한 $\beta$-1,4-D-glucan glucanohydrolase의 특성)

  • 임대식;정춘수;강사욱;하영칠
    • Korean Journal of Microbiology
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    • v.29 no.2
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    • pp.85-91
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    • 1991
  • .betha.-1,4-D-Glucan glucanohydrolase(EC 3.2.1.4;F-II-IV) purified from Trichoderma koningii was identified as a glycoprotein containing 9% carbohydrate. Isoelectric point of the enzyme was estimated to be 4.9 and molecular weight was determined to be approximately 58,000. The porducts of p-nitrophenyl-cellobioside ($PNPG_{2}$) catalyzed by the enzyme were p-nitrophenol(PNP) and p-nitrophenyl-glucoside($PNPG_{1}$). The Km value for $PNPG_{2}$ was estimated to be 0.97 mM in case of the holoside lindage and 10.4 mM in case of the aglycon linkage and their kcat values were $1.8*10^{5}$$ min^{-1}$ and $7.5*10^{5}$ $min^{-1}$ respectively. The product of p-nitrophenyl cellotriose($PNPG_{3}$) was only $PNPG_{1}$. The Km value for $PNPG_{3}$ was 69.5 .$\mu$M and kcat was $1*10^{8}$ $min^{-1}$ which implicates that the enzyme have higher affinity and higher hydrolysis rate toward $PNPG_{3}$ than toward $PNPG_{2}$. The enzyme showed its optimal activity at pH 4.0-4.5 and at 60.deg.C. The effect of gluconolactone on the activity toward $PNPG_{2}$ showed competitive inhibition pattern but glucose and cellobiose did not. The enzyme contained a high content of acidic and hydroxylated amino acids in contrast to basic amino acids.

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Role of LAMMER Kinase in Cell Wall Biogenesis during Vegetative Growth of Aspergillus nidulans

  • Choi, Yu Kyung;Kang, Eun-Hye;Park, Hee-Moon
    • Mycobiology
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    • v.42 no.4
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    • pp.422-426
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    • 2014
  • Depending on the acquisition of developmental competence, the expression of genes for ${\beta}$-1,3-glucan synthase and chitin synthase was affected in different ways by Aspergillus nidulans LAMMER kinase. LAMMER kinase deletion, ${\Delta}lkhA$, led to decrease in ${\beta}$-1,3-glucan, but increase in chitin content. The ${\Delta}lkhA$ strain was also resistant to nikkomycin Z.

Effects of oyster mushroom as a feed addictive in juvenile cherry salmon

  • Oh, Tae-Seok;Park, Youn-Jin;Lim, Hyun-Goo;Jung, Hea-Young;Jang, Myoung-Jun
    • Journal of Mushroom
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    • v.17 no.2
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    • pp.47-51
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    • 2019
  • Our research on juvenile Oncorhynchus masou masou in oyster mushroom supplemented diet was studied to investigate the effect of feeding. Mixing of feed ingredients for dried oyster mushrooms, 3.5, 7.0, 10.5, 14.0 (%) was added to the amount of oyster mushroom dietary beta-glucan content of the more abundant. After the weight of feed given to salmon survey oyster mushrooms diet for 3.5 to 7.0% was similar to the formula feed and the weight of the fish ate oyster mushroom feed over 10.5% were reduced. The oyster mushroom of the experimental diets containing 3.5 to 7.0 percent hepatosomatic index and feed coefficient figure was similar to those of the formulated diets. Therefore we have juvenile cherry salmon fed diets containing 3.5 to 7.0% was considered good to eat and additional research on the immune response will be carried out was necessary.

A Review on Phytochemistry and Pharmacology of Sparassis crispa (꽃송이버섯의 구성성분과 약리효과에 대한 리뷰)

  • Bang, Rodae;Lee, Young-Jong
    • The Korea Journal of Herbology
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    • v.34 no.6
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    • pp.131-138
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    • 2019
  • Objective : Sparassis crispa has been recognized for its therapeutic value since the late 20th century because of its high β-glucan content. Since then, researches have been conducted on the pharmacological effects but most of these are individual studies on the effects of β-glucan from S. crispa and the comprehensive reviews are lacking. The purpose of this study was to review the compounds composition and pharmacological effects of S. crispa. Methods : This review analyzes the papers about chemical and nutritional composition and pharmacological effects of S. crispa. The data in this review is based on selected papers after reviewing all studies containing the keyword "sparassis crispa" for PubMed, NDSL, and J-Stage published before February 2019. Results : S. crispa is composed of protein, lipids, and carbohydrates. Most of the compounds are carbohydrates and the highest content is β-glucan. More than 40% of the dried fruiting body of S. crispa is composed of β-glucan. In addition, it contains polyphenols, flavonoids, terpenoids and phthalide-based compounds. Broad spectrum of its pharmacological actions have been established which include immunomodulatory, anticancer, antiinflammatory, antioxidant, hypoglycemic, antiobesic and neuroprotective effects. Conclusion : The most studied fields have been shown to have immunomodulatory and anticancer effects by inhibiting the proliferation of cancer cells and angiogenesis and increasing hematopoitic responses. Unique structure and characteristic of high molecular weight β-glucan are considered to have high immunomodulatory effects of S. crispa. And low molecular fractions or phthalides of S. crispa also have antioxidant, immunomodulatory and anticancer effects.

Performance evaluation of β-glucan treated lean clay and efficacy of its choice as a sustainable alternative for ground improvement

  • Kumara, S. Anandha;Sujatha, Evangelin Ramani
    • Geomechanics and Engineering
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    • v.21 no.5
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    • pp.413-422
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    • 2020
  • The choice of eco-friendly materials for ground improvement is a necessary way forward for sustainable development. Adapting naturally available biopolymers will render the process of soil stabilization carbon neutral. An attempt has been made to use β-glucan, a natural biopolymer for the stabilization of lean clay as a sustainable alternative with specific emphasis on comprehending the effect of confining stresses on lean clay through triaxial compression tests. A sequence of laboratory experiments was performed to examine the various physical and mechanical characteristics of β-glucan treated soil (BGTS). Micro-analysis through micrographs were used to understand the strengthening mechanism. Results of the study show that the deviatoric stress of 2% BGTS is 12 times higher than untreated soil (UTS). The micrographs from Scanning Electron Microscopy (SEM) and the results of the Nitrogen-based Brunauer Emmett Teller (N2-BET) analysis confirm the formation of new cementitious fibres and hydrogels within the soil matrix that tends to weld soil particles and reduce the pore spaces leading to an increase in strength. Hydraulic conductivity (HC) and compressibility reduced significantly with the biopolymer content and curing period. Results emphases that β-glucan is an efficient and sustainable alternative to the traditional stabilizers like cement, lime or bitumen.