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Isolation and Characterization of a Water-Soluble Polysaccharide from the Mycelia of Solid Cultured Agaricus blazei Murill  

Chung, Ha-Yull (Department of Food Science & Biotechnology, and Food & Biotechnology Research Center, Hankyong National University)
Cho, Young-Jun (Department of Food Science & Biotechnology, and Food & Biotechnology Research Center, Hankyong National University)
Kim, Tae-Wook (Department of Food Science & Biotechnology, and Food & Biotechnology Research Center, Hankyong National University)
Publication Information
Food Science and Biotechnology / v.14, no.2, 2005 , pp. 259-262 More about this Journal
Abstract
A fraction (CMEx-AH-${\beta}$) of water-soluble polysaccharides, showing selective antitumor activities, was isolated from the mycelia of solid cultured Agaricus blazei Murill by hot-water extraction, ethanol precipitation, and series of chromatography. Chemical characteristics of CMEx-AH-${\beta}$, were as follows: carbohydrate content, 48%; monosaccharide composition, Man:Glu:Gal (2:93:5); molecular weight, $2{\times}10^5$; uronic acid content, 6.2%. Fundamental structure of CMEx-AH-${\beta}$, was deduced as ${\beta}-(1\;{\rightarrow}\;6)$-D-glucan with ${\beta}-(1\;{\rightarrow}\;3)$-D-glucosidic side chains based on results of methylation and $^{13}C$ NMR spectroscopic analyses.
Keywords
Agaricus blazei Murill; chemical characteristics; fundamental structure;
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