• Title/Summary/Keyword: $\mu$-Synthesis

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The Effect of Salicylic Acid on the Accumulation of $Cd^{2+}$ and Nonprotein-SH Synthesis in Roots and Epidermal Strips of Commelina Communis L. (닭의장풀에 $Cd^{2+}$처리 시 $Cd^{2+}$ 흡수와 nonprotein-SH 합성에 미치는 살리실릭산의 영향)

  • 이준상
    • Korean Journal of Environmental Biology
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    • v.19 no.3
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    • pp.218-222
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    • 2001
  • Three weeks old Commelina was cultivated in Hoagland solution with or without $(\pm{100\mu{M}\;Cd^{2+}$ and with $100\mu{M}\;Cd^{2+}+100\mu{M\; SA)$ for 4 days. The contents of $Cd^{2+}$ and nonprotein-SH synthesis in roots and isolated epidermal strips were measured. In the treatment of $Cd^{2+}$, the concentration of $Cd^{2+}$ was $98\mu{M}\;Cd^{2+}$/g.fr.wt and it was 6 times higher than that of control. $Cd^{2+}$+SA increased 1.2 times higher concentration of $Cd^{2+}$ than that of the treatment ofTEX>$Cd^{2+}$ alone. In the treatment of TEX>$Cd^{2+}$, nonprotein-SH also increased 2 times higher than that of the control. TEX>$Cd^{2+}$ + SA increased 2 times higher concentration of nonprotein-SH than that of the treatment of TEX>$Cd^{2+}$ as well. In case of isolated epidermal strips, the response was similar with the roots. Therefore, it suggest that the effect of salicylic acid on the accumulation of TEX>$Cd^{2+}$ and nonprotein-SH synthesis in roots, and isolated epidermal strips was related with an increase of the membrane permeability by SA and that SA may be positively involved in the process of nonprotein- SH synthesis.

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Biological monitoring of dye manufacturing workers by hemoglobin adducts (헤모글로빈 부가체를 이용한 염료제조 근로자의 노출평가)

  • Jhang, Kyuyeub;Lee, Keungjong;Kim, Chinyon;Yoon, youngshik;Roh, Jaehoon
    • Journal of Korean Society of Occupational and Environmental Hygiene
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    • v.10 no.2
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    • pp.124-139
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    • 2000
  • This study was performed to investigate monoacetylbenzidine(MABZ) and benzidine(BZ) hemoglobin adducts among workers who worked at benzidine based dye manufacturing company, and exposed by benzidine and benzidine based dye. The hemoglobin adducts were compared with work environment assessment result for evaluating the usefulness of biological monitoring. The mean BZ hemoglobin adducts among the first synthesis worker's hemoglobin adducts were $40.69{\mu}gBZ/g$ Hb and those of dry and packing workers were $22.14{\mu}gBZ/g$ Hb. The mean of MABZ hemoglobin adducts among 1st synthesis workers were $255.84{\mu}gMABZ/g$ Hb, dispersion worker's hemoglobin adducts were $76.17{\mu}gMABZ/g$ Hb and synthesis worker's hemoglogin adducts were $28.66{\mu}gMABZ/g$ Hb. Work environment assessment results during past 3 years were $0.0065mg/m^3$ and $0.5659mg/m^3$ of benzidine based dye concentration in ambient air of drying and packing only. Dye producing process was categorized by the possibility of exposure to benzidine and benzidine based dye. BZ and MABZ hemoglobin adducts were $19.55{\mu}gBZ/g$ Hb, $119.80{\mu}gMABZ/g$ Hb among workers who exposed by benzidine dihydrochloride and $16.32{\mu}gBZ/g$ Hb, $316.56{\mu}gMABZ/g$ Hb among workers who exposed by benzidine based dye. BZ hemoglobin adducts were not detected among control group and MABZ hemoglobin adducts were $5.33{\mu}gMABZ/g$ Hb. The differences between control and other exposed group was statistically significant. But there was no statistically significant differences between benzidine dihydrochloride exposed process and benzidine based dye exposed group. BZ and MABZ hemoglobin adducts were $2.23{\mu}gBZ/g$ Hb, $76.17{\mu}gMABZ/g$ Hb and $3.46{\mu}gBZ/g$ Hb, $21.33{\mu}gMABZ/g$ Hb. So hemoglobin adducts of MABZ were 5 ~ 30 time higher than those of BZ(P<0.003). Above results indicate that work environment assessment didn't detected benzidine and benzidine based dye in ambient air but biological monitoring detected those of hemoglobin adducts. Two group's hemoglobin adducts exposed benzidine dihydrochloride and benzidine based dye were high level but wasn't statistically significant and those were not detected in control group.

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Changes in Protein Synthesis Induced by Chilling in Tomato Chloroplasts

  • Kim, Won-Il;Jung, Goo-Bok;Kim, Min-Kyeong;Park, Kwang-Lai;Yun, Sun-Gang
    • Korean Journal of Environmental Agriculture
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    • v.20 no.5
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    • pp.310-316
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    • 2001
  • To find out the effect of low temperature on the regulation of tomato chloroplast genes, the optimization of the system in chloroplast protein synthesis and the identification of the changes in chloroplast protein synthesis induced by chilling were studied. Incorporation reaction occurred rapidly at the first 30 minutes and was constantly maintained after 60 minutes. A broad optimal temperature on protein synthesis was found around 20 to $30^{\circ}C$. No difference was shown in the chloroplast protein synthesis under high light intensity (1600 ${\mu}E/m^2/s$) as well as under low light intensity (400 ${\mu}E/m^2/s$) even darkness. $K^+$, $Mg^{++}$ and ATP at an optimal concentration act as an activator, while DTT, chloramphenicol, cycloheximide, $Ca^{++}$ and inorganic phosphate act as an inhibitor in the chloroplast protein synthesis. Synthesis of 15, 55 and 60 kd chloroplast encoded stromal proteins and 18, 24, 33 and 55 kd chloroplast encoded thylakoid membrane proteins were reduced by chilling, while 17 kd chloroplast encoded stromal protein and 16 kd chloroplast encoded thylakoid membrane protein was induced by chilling. It was expected that the 55 kd stromal protein would be the large subunit of rubisco and the 33 kd thylakoid membrane protein would be the D1 protein which was drastically reduced by chilling.

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The Preparation of PZT Fine Powder Using Ammonia Gas as a Precipitator (Ammonia gas를 침전제로 이용한 PZT 미분말의 합성)

  • 현성호;김정환;이한철;허윤행
    • Journal of environmental and Sanitary engineering
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    • v.14 no.1
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    • pp.62-69
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    • 1999
  • In this study, the synthesis of PZT powder by bubble column reactor was investigated at various reaction conditions. As a result, the volume % of $NH_3$ gas used as a precipitator had no effect on the synthesis, but the more research is needed to control particle size.As a carrier gas, Ar, $O_2$ and air only increased the stirring effect but had no effect chemically on the synthesis. The calcination temperature of prepared PZT powder was about $500-600^{\circ}C$ and the meanparticle size of synthesized PZT powder was about $0.17{\mu}m$. The grain size of sintered body is about $0.5~3{\mu}m$ and this is similar with the value of commercial products.

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Synthesis and Antiproliferative Activity of Quinazolinylmethoxybenzene Derivatives against Melanoma Cell Line (Quinazolinylmethoxybenzene 유도체 합성 및 흑색종 세포증식 저해효능)

  • Lee, Jun-Sang;Yoo, Kyung-Ho
    • Journal of the Korean Applied Science and Technology
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    • v.27 no.1
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    • pp.20-28
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    • 2010
  • Melanoma is the most aggressive form of skin cancer and is the fastest growing type of cancer in the United States. We report here the synthesis of a novel series of quinazolinylmethoxybenzene derivatives 1a-c and their antiproliferative activities against A375 human melanoma cell line. Among them, urea compound 1a ($IC_{50}\;=\;4.8\;{\mu}M$) having 4-chloro-3-trifluoromethylphenyl moiety showed superior antiproliferative activity to Sorafenib ($IC_{50}\;=\;5.5\;{\mu}M$) as a reference compound. These results will helpful for designing structure of a therapeutic agent for the treatment of melanoma.

Antiviral Activity of Papaverine and Nucleoside Analogs on the Human Cytomegalovirus Infection (Human Cytomegalovirus 감염에 대한 파파베린과 뉴클레오사이드 유사체의 항바이러스 효과)

  • ;Albrecht, T.
    • Korean Journal of Microbiology
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    • v.29 no.1
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    • pp.25-33
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    • 1991
  • Antiviral activities of papaverine and nucleoside analogs, 9-[(1,3-dihydroxy-2-propoxy)methyl]guanine (DHPG) and acyclovir, against human cytomegalovirus (HCMV) infection were compared in vitro. Papaverine and DHPG were effective in reducing infectious HCMV yields with $ED_{50}{\s}$ (effective dose 50: the concentraion at which 50% of virus yields was obtained) of approximately 1.02 and $0.45{\mu}{\M}$, respectively; while acyclovir was less effective with an $ED_{50}$ of about $10.4{\mu}{\M}$The relative cytotoxicity of these drugs was evaluated under the same conditions used to measure infectious HCMV yields. Papaverine and DHPG demonstrated little cellular toxicity as measured by their effect on the viability of confluent cells at concentrations in the range of those demonstrating potent inhibition of HCMV replication. Similarly, protein synthesis was largely unaffected by these drugs in stationary mock-infected cells as measured by the incorporation of isotopically labelled amino acids. In contrast, cellular DNA synthesis was invariably reduced in the presence of either drug. HCMV-specific DNA synthesis was also strongly inhibited by papaverine and DHPG.

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A Robust Controller Design for the Position Control of a Spring-Mass System (탄성-질량시스템의 위치제어를 위한 강건 제어기 설계)

  • 박종우;이상철
    • Journal of the Korean Institute of Telematics and Electronics T
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    • v.36T no.3
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    • pp.41-49
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    • 1999
  • In this paper, we design a controller using the $\mu$-synthesis method and apply it for the spring-mass system with noncollocated sensors and actuators. We assume that the values of the spring stiffness and load mass of the plant are uncertain. The plant is modeled with parametric uncertainty by using the state space equation, especially the descriptor form. The $H_\infty$ controller designed by the $\mu$-synthesis method is compared with the standard $H_\infty$ controller To compare performances of two $H_\infty$ controllers, it is assumed that both controllers were designed with same weighting functions except that the $\mu$-synthesis controller has structured uncertainties. By compared with the standard $H_\infty$ controller, we show that the designed controller has satisfactory robust performance as well as robust stability by simulations and experiments.

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Antioxidant and Skin Anti-Aging Effects of Marigold Methanol Extract

  • Kang, Chul Ho;Rhie, Sung Ja;Kim, Young Chul
    • Toxicological Research
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    • v.34 no.1
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    • pp.31-39
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    • 2018
  • The objective of this study was to evaluate the antioxidant and anti-aging effects of marigold methanol extract (MGME) in human dermal fibroblasts. Total polyphenolic and flavonoid contents in MGME were 74.8 mg TAE (tannic acid equivalent)/g and 85.6 mg RE (rutin equivalent)/g, respectively. MGME ($500{\mu}g/mL$) increased 1,1-diphenyl-2-picryl hydrazyl (DPPH) and 2,2'-azino-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical-scavenging, and superoxide dismutase (SOD)-like antioxidant activities by 36.5, 54.7, and 14.8%, respectively, compared with the control. At $1,000{\mu}g/mL$, these activities increased by 63.7, 70.6, and 20.6%, respectively. MGME ($100{\mu}g/mL$) significantly increased the synthesis of type 1 procollagen by 83.7% compared with control treatment. It also significantly decreased Matrix Metalloproteinase-2 (MMP-2) activity and MMP-1 mRNA expression by 36.5% and 69.5%, respectively; however, it significantly increased laminin-5 mRNA expression by 181.2%. These findings suggest that MGME could protect human skin against photo-aging by attenuating oxidative damage, suppressing MMP expression and/or activity as well as by stimulating collagen synthesis.

Inhibitory Effects of Plant Extracts on Tyrosinase Activity and Melanin Synthesis

  • Park, Hyen-Joo;Park, Kwang-Kyun;Hwang, Jae-Kwan;Chung, Won-Yoon;Lee, Seung-Eun;Lee, Sang-Kook
    • Natural Product Sciences
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    • v.16 no.2
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    • pp.133-139
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    • 2010
  • In order to develop a new skin whitening agent, approximately 100 plant extracts were evaluated for their inhibitory activities against melanin biosynthesis in cultured mouse melanocyte melan-a cells. As a result, seven extracts exhibited over 50% inhibition of melanin synthesis compared to control at a concentration of $20\;{\mu}g/ml$. In particular, Aster ageratoides Turcz. var. ageratoides (branch, root, aerial, flower; $IC_{50}$ = 17.3, 6.1, 13.6, $12.9\;{\mu}g/ml$, respectively) and Physalis alkekengi var. francheti (leaf, unripen fructus, aerial; $IC_{50}$ = 6.5, 28.3, $23.9\;{\mu}g/ml$) markedly inhibited melanin synthesis. In addition, tyrosinase activity was monitored by the measurement of dopachrome formation from the oxidation of L-3,4-dihydroxyphenylalanine. Extracts of A. ageratoides Turcz. var. ageratoides (flower) and P. alkekengi var. francheti (leaf) showed the most potent tyrosinase inhibitory activity. These plants might be the potential candidate sources in the development of novel skin-whitening products.

An Experimental Study on the Effect of Angelica gigas Ethanol Extract on Hyaluronic Acid Synthesis (Angelica gigas 에탄올 추출물의 Hyaluronic acid 합성 효과에 대한 실험적 연구)

  • Park, Hye-Su;Ha, Hun-Yong;Kim, Hee-Taek
    • The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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    • v.31 no.1
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    • pp.32-41
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    • 2018
  • Objectives : Hyaluronic acid(HA) is a mucopolysaccharide, occuring naturally in living organisms. It is one of the most hydrophilic molecules, so it has been known as being related to skin hydration and skin aging. The purpose of this study is to examine the effects of Angelica gigas(A. gigas) ethanol extract on hyaluronic acid synthesis. Methods : To determine cytotoxicity and hyaluronic acid synthase 2 gene expression, hyaluronic acid production in HaCaT cells, MTT assay and RT-PCR ELISA was used. Results : There were no cytotoxicity in $50{\mu}g/ml$ concentration A. gigas extract in MTT assay. Hyaluronic acid synthase 2(HAS2) gene expression was increased by all treated concentration A. gigas extract. Hyaluronic acid production was higher than control group in $50{\mu}g/ml$ & $100{\mu}g/ml$ concentration A. gigas extract. Conclusions : Hyaluronic acid production was increased by A. gigas extracts. Therefore, We suggest that A. gigas can make a contribution to the moisturizing effect on human skin.