The Effect of Salicylic Acid on the Accumulation of $Cd^{2+}$ and Nonprotein-SH Synthesis in Roots and Epidermal Strips of Commelina Communis L.

닭의장풀에 $Cd^{2+}$처리 시 $Cd^{2+}$ 흡수와 nonprotein-SH 합성에 미치는 살리실릭산의 영향

  • 이준상 (상지대학교 생명과학과)
  • Published : 2001.09.01

Abstract

Three weeks old Commelina was cultivated in Hoagland solution with or without $(\pm{100\mu{M}\;Cd^{2+}$ and with $100\mu{M}\;Cd^{2+}+100\mu{M\; SA)$ for 4 days. The contents of $Cd^{2+}$ and nonprotein-SH synthesis in roots and isolated epidermal strips were measured. In the treatment of $Cd^{2+}$, the concentration of $Cd^{2+}$ was $98\mu{M}\;Cd^{2+}$/g.fr.wt and it was 6 times higher than that of control. $Cd^{2+}$+SA increased 1.2 times higher concentration of $Cd^{2+}$ than that of the treatment ofTEX>$Cd^{2+}$ alone. In the treatment of TEX>$Cd^{2+}$, nonprotein-SH also increased 2 times higher than that of the control. TEX>$Cd^{2+}$ + SA increased 2 times higher concentration of nonprotein-SH than that of the treatment of TEX>$Cd^{2+}$ as well. In case of isolated epidermal strips, the response was similar with the roots. Therefore, it suggest that the effect of salicylic acid on the accumulation of TEX>$Cd^{2+}$ and nonprotein-SH synthesis in roots, and isolated epidermal strips was related with an increase of the membrane permeability by SA and that SA may be positively involved in the process of nonprotein- SH synthesis.

3주간 생장한 닭의장풀을 Hoagland용액 $(\pm{100\mu{M}\;Cd^{2+}$ 또는 $100\mu{M}\;Cd^{2+}+100\mu{M\; SA)$에서 4일 동안 수경 재배한 후 $Cd^{2+}$ 및 살리실릭산(SA) 처리시, 닭의장풀의 뿌리와 분리 표피에서의 $Cd^{2+}$ 농도와 nonprotein-SH 합성을 측정하였다. $Cd^{2+}$ 처리구에서는 $98\mu{M}\;Cd^{2+}/g.fr.wt$이 검출되었다. 이는 대조구에 비해 약 6배 증가한 것이다. $Cd^{2+}$을 SA와 같이 처리한 경우$Cd^{2+}$만 처리한 경우보다 뿌리에서 약 1.2배의 $Cd^{2+}$이 증가하였다. $Cd^{2+}$처리는 nonprotein-SH농도를 약 2배 이상 증가시켰다. $Cd^{2+}$을 SA와 같이 처리한 경우 $Cd^{2+}$만 처리한 경우보다 뿌리에서 nonprotein-SH농도는 약 2배 증가되었다. 분리표피에서 $Cd^{2+}$+SA 처리는 $Cd^{2+}$ 처리구에 비해 $Cd^{2+}$농도를 약 1.1배 증가시켰으며, 뿌리에서는 약 1.2배 증가시켰다. Nonprotein-SH도 합성도 뿌리와 비슷하게 $Cd^{2+}$농도에 의존하며 증가되었으며, $Cd^{2+}$+SA처리는 nonprotein-SH합성을 $Cd^{2+}$처리구에 비해 약 2배 증가시켰다. 위 결과로부터 신호전달물질인 SA처리는 $Cd^{2+}$ 흡수를 촉진시키며, 아울러 non-protein-SH 합성 과정에도 영향을 주는 것으로 사료된다.

Keywords

References

  1. Korean J. Ecology v.5 no.4 Studies on the effect of heavy metal on the growth of various plants Kim BW
  2. J. Ecol. v.15 no.4 Study on the resistance of various herbaceous plants to the effects of heavy metals-Responses of plants to soil treated with cadmiun and lead Kim BW;JS Park
  3. 상지대학교 자연과학 논총 Ecological study on the effect og heavy metals to the vascular plants Kim BW
  4. Science v.263 Surprising signals in plant cells Jones AM
  5. J. Plant Biol. v.41 no.2 The mechanism of stomatal closing by salicylic aicd in Commelina communis L. Lee JS
  6. 환경생물학회지 v.18 no.1 닭의장풀 내 $Cd^{2+}$의 분포와 생리적 독성 Lee JS
  7. The Plant J. v.2 Salicylic acid and plant disease resistance Malamy J;DF Klessig
  8. Planta v.186 Photoinhibition of photosynthesis represents a mechanism for the longterm regulation of photosystem Ⅱ Oquist G;WS Chow;JM Anderson
  9. J. Environ. Quality v.1 Cadmium absorption and growth of various plant species as by solution cadimum concentration Page AL:FT Bingham;C Nelson
  10. Annu. Rev. Biochem. v.59 Phytochyletins Rauser WE
  11. Biochem. J. v.295 Plant metallothioneins Robison NJ;AM Tommey;C Kuske;PJ Jackson
  12. Stomata Willmer CM