The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology (한방안이비인후피부과학회지)

The Society of Korean Medicine Ophthalmology, Otolaryngology & Dermatology (대한한방안이비인후피부과학회)
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An Experimental Study on the Effect of Angelica gigas Ethanol Extract on Hyaluronic Acid Synthesis

Angelica gigas 에탄올 추출물의 Hyaluronic acid 합성 효과에 대한 실험적 연구

  • Park, Hye-Su (Department of Korean Medical Ophthalmology & Otolaryngology & Dermatology, College of Korean Medicine, Semyung University) ;
  • Ha, Hun-Yong (Department of Pharmaceutical Science & Engineering, Seowon University) ;
  • Kim, Hee-Taek (Department of Korean Medical Ophthalmology & Otolaryngology & Dermatology, College of Korean Medicine, Semyung University)
  • 박혜수 (세명대학교 한의과대학 한방안이비인후피부과학교실) ;
  • 하헌용 (서원대학교 제약공학과) ;
  • 김희택 (세명대학교 한의과대학 한방안이비인후피부과학교실)
  • Received : 2018.01.09
  • Accepted : 2018.02.09
  • Published : 2018.02.25
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Abstract

Objectives : Hyaluronic acid(HA) is a mucopolysaccharide, occuring naturally in living organisms. It is one of the most hydrophilic molecules, so it has been known as being related to skin hydration and skin aging. The purpose of this study is to examine the effects of Angelica gigas(A. gigas) ethanol extract on hyaluronic acid synthesis. Methods : To determine cytotoxicity and hyaluronic acid synthase 2 gene expression, hyaluronic acid production in HaCaT cells, MTT assay and RT-PCR ELISA was used. Results : There were no cytotoxicity in $50{\mu}g/ml$ concentration A. gigas extract in MTT assay. Hyaluronic acid synthase 2(HAS2) gene expression was increased by all treated concentration A. gigas extract. Hyaluronic acid production was higher than control group in $50{\mu}g/ml$ & $100{\mu}g/ml$ concentration A. gigas extract. Conclusions : Hyaluronic acid production was increased by A. gigas extracts. Therefore, We suggest that A. gigas can make a contribution to the moisturizing effect on human skin.

Keywords

References

  1. KDA Textbook Editing Board. Textbook of Dermatology. 6th edition. Seoul:medbook. 2014:2-3,697-703.
  2. Yu HY, Yang IJ, V.R Lincha, Park IS, Lee DU, Shin HM. The Effects of the Fruits of Foeniculum vulgare on Skin Barrier Function and Hyaluronic Acid Production in HaCaT Keratinocytes. Journal of Life Science. 2015;25(8):880-8. https://doi.org/10.5352/JLS.2015.25.8.880
  3. Park CS. Epidermal Homeostasis and Dry Skin Management. J Soc Cosmet Scientists Korea. 2008;34(1):1-8.
  4. Kim DS, Jeon BK, Mun YJ, Kim YM, Lee YE, Woo WH. Effect of Dioscorea Aimadoimo on Anti-aging and Skin Moisture Capacity. Korean J Oriental Physiology & Pathology. 2011;25(3):425-30.
  5. Text of Traditional Korean Dermatology & Surgery. Busan:Sunwoo Publisher. 2007:58-9.
  6. Oriental Medical Terminology. Seoul: Hamchunhanhak. 2006:342.
  7. Choi JH, Kim HM, Song YS, Park SG, Kim JJ, Lee CK. Physical Effects of the Cosmetic Product Containing of Saccharomyces Fermented Modified Kyungohkgo Extract on Human Skin. Kor J Herbology. 2007;22(4):227-32.
  8. Kim KT, Kim YH, Kim JG, Han CS, Park SH, Lee BY, et al. Preparation of Oligo Hyaluronic Acid by Hydrolysis and Its Application as a Cosmetic Ingredient. J Soc Cosmet Scientists Korea. 2007;33(3):189-96.
  9. Kim SH, Nam GW, Kang BY, Lee HK, Moon SJ, Chang IS. The Effect of Kaempferol, Quercetin on Hyaluronan-Synthesis Stimulation in Human Keratinocyte (HaCaT). J Soc Cosmet Scientists Korea. 2005;31(1):97-102.
  10. Song HJ, Mu HJ, Lee SH. Effect of Ferulic Acid Isolated from Cnidium Officinaale on the Synthesis of Hyaluronic Acid. J Soc Cosmet Scientists Korea. 2013;39(4):281-8. https://doi.org/10.15230/SCSK.2013.39.4.281
  11. Lee PJ, Kim HT, Yoon KS, Park HC, Ha HY. The effect of Astragalus membranaceus methanol extract on hyaluronic acid production in HaCaT cells. The Journal of Korean Medical Ophthalmology & Otolaryngology & Dermatology. 2013;26(1):75-81. https://doi.org/10.6114/jkood.2013.26.1.075
  12. Kang MS, Ha HY, Kim HT. An Experimental Study on the Effect of Angelica acutiloba Ethanol Extract on Hyaluronic Acid Synthesis. J Korean Med Ophthalmol Otolaryngol Dermatol. 2015;28(1):32-40. https://doi.org/10.6114/jkood.2015.28.1.032
  13. Kim SA, Oh HK, Kim JY, Hong JW, Cho SI. A Review of Pharmacological Effects of Angelica gigas, Angelica sinensis, Angelica acutiloba and their Bioactive Compounds. J Korean Oriental Med. 2011;32(4):1-24.
  14. Roh SS, Lee KN. Effects of Dangkiyeumja Water Extract on the Anti-Allergic Responses and the Functions of Murine Immunocytes. J Korean Med Ophthalmol Otolaryngol Dermatol. 1991;4(1):23-42.
  15. Choi JH. Cytotoxicity of water extract of Dangkwieumja ka Sumsoo on A43l Cells. J Korean Med Ophthalmol Otolaryngol Dermatol. 1996;9(1):1-15.
  16. Roh SS, Lee KH. Research of Experimental Kamidangkwieumja in Psoriasis. J Korean Med Ophthalmol Otolaryngol Dermatol. 1999;12(1):113-42.
  17. Shim SH, Kim JH, Choi JH. A Clinical study about the effect of Danguiumjagagam on a Psoriasis Patient. J Korean Med Ophthalmol Otolaryngol Dermatol. 2002;15(1):336-42.
  18. Kim SH, Choi JH, Kim JH, Park SY. Effect of Tang-gwi-eum-za-gagambang along with External Spray Therapy on the Spontaneously Occurring Atopic Dermatitis Development in NC/Nga Mouse. J Korean Med Ophthalmol Otolaryngol Dermatol. 2005;18(1):27-49.
  19. Kim CH, Kwon MC, Han JG, Na CS, Kwak HG, Choi GP, et al. Skin-Whitening and UV-Protective Effects of Angelica gigas Nakai Extracts on Ultra High Pressure Extraction Process. Korean J Medicinal Crop Sci. 2008;16(4):255-60.
  20. Hwang SY, Lee JT, Kim YU, Kim HJ. Skin Whitening Effects of Extracts from Angelicae Gigantis Radix and Lycii fructus Ethanol Extracts. Herbal Formula Science. 2013;21(1):91-8. https://doi.org/10.14374/HFS.2013.21.1.091
  21. Lee JH, Lee S, Kim MG, Kim MH, Kim HJ, Jo HJ, et al. Effects of Angelica Gigantis Radix Extracts on the Collagenase Activity and Procollagen Synthesis in HS68 Human Fibroblasts and Tyrosinase Activity. Kor J Herbology. 2011;26(1):29-33. https://doi.org/10.6116/KJH.2011.26.1.029
  22. Kim JH, Kim CH, Kim HS, Kwon MC, Song YK, Seong NS, et al. Effect of Aqueous Extracts from Rubus coreanus Miquel and Angelica gigas Nakai on Anti-tumor and Anti-stress activities in mice. Korean J Medicinal Crop Sci. 2006;14(4):206-11.
  23. Park SK, Hong SK, Kim HJ, Kim BY, Kim T, Kang JS, et al. Cosmetic effect of Angelica gigas Nakai root extracts. Korean Chem Eng Res. 2009;47(5):553-7.
  24. Sim GS, Lee DH, Kim JH, An SK, Choe TB, Kwon TJ, et al. Black rice (Oryza sativa L. var. japonica) hydrolyzed peptides induce expression of hyaluronan synthase 2 gene in HaCaT keratinocytes. J Microbiol Biotechnol. 2007;17(2):271-9.
  25. Han JM, Kang NR, Ko WS, Yoon HJ. The Study on the Korean and Western Medical Literatures for Skin Aging wrinkle, hyperpigmentation, dry skin, facial flush. J Korean Med Ophthalmol Otolaryngol Dermatol. 2014;27(2):1-13. https://doi.org/10.6114/JKOOD.2014.27.2.001
  26. Lee JG, Lee JS, Park HJ, Cho WJ, Kim MR, Ha ID, et al. The moisturizing Effects of the Cosmetic Products Containing Herbs Extract on Infant Skin. J Korean Med Ophthalmol Otolaryngol Dermatol. 2008;21(3):124-31.
  27. Jeong YS, Ha HY. Effect of Scutellariae Radix Ethanol Extract on Hyaluronic Acid Synthesis. Journal of Investigative Cosmetology. 2015;11(4):309-13. https://doi.org/10.15810/jic.2015.11.4.004
  28. Hong CH, Lee SJ. Study on the Moisturizing Effects of Puerariae Radix Ethanol Extract. J Korean Med Ophthalmol Otolaryngol Dermatol. 2017;30(1):106-17. https://doi.org/10.6114/jkood.2017.30.1.106
  29. Park WS, Lee CH, Soh KS, Lee YJ, Lee CY, Lee TH, et al. Study on Biophoton Emission from roots of Angelica gigas N., Angelica sinensis D., and Angelica acutiloba K. The Korea journal of herbology. 2007;22(4):95-100.
  30. Kang SA, Jang KH, Lee JE, Ahn DK, Park SK. Differences of Hematopoietic Effects of Angelica gigas, A. sinensis and A. acutiloba Extract on Cyclophosphamide-induced Anemic Rats. Korean J Food SCI Technol. 2003;35(6):1204-8.
  31. Lee JJ, Kim AR, Seo YN, Lee MY. Comparison of Physicochemical Compositionof Three Species of Genus Angelica. Korean J Food Preserv. 2009;16(1):94-100.
  32. Oh HS. Comparative studies on the angiogenic activity of water extract of Angelica Gigantis Radix, Angelica Sinensis Radix and Angelica Radix. The Korea journal of herbology. 2001;16(2):19-27.