• 한국식품영양과학회지
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• 제30권1호
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• pp.119-126
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• 2001

Effects of Sardine Oil Feeding and Vitamin E Supplementation on Histopathological Changes and $\alpha$-L-fucosidase activity in experimental hepatocarcinogenesis. Sprague-Dawley rats weighing 80~90 g were fed the diet containing either 15% corn oil (CO) or sardine oil (SO) with or without vitamin E supplements (dl-$\alpha$-tocopherol acetate 800 IU/kg diet) for 8 weeks. After 2 weeks of feeding, the rats were given a single intraperitoneal injectin of diethylnitrosamine (DEN, 200 mg/kg BW). From the fifth week, rats were given 0.02% acetylaminofluorene (AAF) in diet for 4 weeks. At the seventh week, 0.05% phenobarbital in liver and hepatic glutathione S-transferase palcental form positive (GST-P+) foci were examined by Hematoxylin& Eosin (H&E) staining and immunohistochemical method, respectively. Serum $\alpha$-L-fucosidase activity was determined. The livers fromt he carcinogen treated rats showed significantly increased formation of GST-P+ foci at sacrifice points while the livers fromthe non-carcinogen treated groups showed almost no foci. Although GST-P+ foci formation was not affected by dietary oil, it was increased unexpectedly by vitamin E supplementation. Histopathological changes were similar to patterns of GST-P+ foci formation in almost all groups. Serum $\alpha$-L-fucosidase activities were increased by carcinogen treatment in all dietary groups. $\alpha$-L-fucosidase activities were positively correlated with GST-P+ foci formation. There results suggest that excessive vitamin E supplementation can enhance hepatocarcinogenesis although the mechanisms involved are not clearly understood.

• Asian-Australasian Journal of Animal Sciences
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• 제20권3호
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• pp.351-358
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• 2007

Carbohydrate-protein interactions are known to be important in gamete interactions. Several evidence indicated that a fucose-containing sulfated polysaccharide fucoidan was potential inhibitor of fertilization in vitro and thus fucose seemed to be part of the recognition signal of gamete interaction in mammals. In recent investigation we found that ${\alpha}$-L-fucosidase activity was present in boar spermatozoa and it was related to sperm binding to and penetration into zona pellucida (ZP) in vitro. The objective of this study was to determine the effects of monosaccharide L-fucose and polysaccharide fucoidan on sperm ${\alpha}$-L-fucosidase activity and relation to sperm-oocyte interaction in pig. Results indicated that the activity of sperm ${\alpha}$-L-fucosidase was largely inhibited (62%) when sperm suspension was treated with monosaccharide L-fucose. It also significantly inhibited the number of sperm binding to ZP (32%) and penetration into zona-intact oocytes (72%), but did not inhibit penetration into zona-free oocytes when fertilization medium contained L-fucose. The chlorotetracycline (CTC) assessment showed that L-fucose did not affect induction of sperm capacitation and acrosome reaction. In contrast, the activity of sperm ${\alpha}$-L-fucosidase was not inhibited when sperm suspension was treated with polysaccharide fucoidan but sperm-ZP binding was greatly inhibited (85%) and completely blocked sperm penetration into zona-intact or zona-free oocytes. The CTC assessment showed that fucoidan increased the F pattern and decreased the AR pattern sperm. These results suggested that the different inhibitory mechanisms were present between monosaccharide L-fucose and polysaccharide fucoidan on sperm-oocyte interaction, the inhibition effect of ${\alpha}$-L-fucose on sperm binding and penetrating into ZP caused sperm ${\alpha}$-L-fucosidase inhibited by ${\alpha}$-L-fucose.

• Archives of Pharmacal Research
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• 제19권4호
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• pp.307-311
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• 1996

The activity of fucosidase shows different value depending on disease, eg) fucosidosis and I cell disease are characterized by the absence or deficiences of .alpha.-fucosidase, and sera of ovarian cancer patients exhibited a statistically significant deficiency of .alpha.-L-fucosidase activity (Zielke et al., 1972; Kress et al., 1980; Barlow et al., 1981). For the purpose of diagnosis of these disease easily, the manual of test can be developed by preparing kits of hydrophobic-binding substrate of fucosidase that bind C18-column.

• KSBB Journal
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• 제20권5호
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• pp.355-358
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• 2005

Bacteroides fragiles Roid8은 인간의 장내에서 가장 우세한 균종에 속하는 것으로서 효소활성을 측정해본 결과 다른 균들에 비해 $\alpha-fucosidase$의 활성이 높은 것으로 나타났다. 본 연구실에서 조사된 $\alpha-fucosidase$의 생산 최적배지 조성은 1L 배지당 BHI 37g, hemin 10mg, cysteine 0.5g, resazurin 1mg, vitamin K 1mg, starch 5g을 함유하였다. 또한 DEAE-sepharose와 CM-cellulose 크로마토그래피를 거쳐 조효소액을 분리하여 조사한 결과 효소의 최적 반응온도는 $40^{\circ}C$, 최적반응 pH는 7.0이었다. 무기 금속이온의 영향은 $Co^{++}\;and\;Zn^{++}$ 존재 하에서 효소역가가 낮아졌다.

• Journal of Ginseng Research
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• 제24권2호
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• pp.89-93
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• 2000

6년생 고려인삼근(panax ginseng C.A. Meyer)중 수종의 exo-O-glycosylhydrolase 활성을 중심부와 주피부로 나누어 생육시기별로 조사하였다. $\alpha$-D-galactosidase, $\beta$-D-galactosidase, $\alpha$-L-man-nosidase , N-acetyl-$\beta$-D-giucosarninidase, $\alpha$-D-galactosidase, $\alpha$-L-arabinosidase와 $\beta$-D-fucosidase는 중심부와 주피부에서 모두 활성이 있으나 $\beta$-L-mannosidase, $\alpha$-D-xylosidase, $\beta$-D-xylosidase, $\alpha$-D-rhamnosidase와 $\beta$-D-glucosidase의 효소활성은 검색되지 않았다. $\beta$-D-galactosidase의 활성은 연중 높게 유지되었고 $\alpha$-L-mannosidase의 활성도 높은 경향이었다. 인삼근중 탄수화물 대사효소의 활성은 생육시기와 환경조건 및 부위에 따라 매우 다른 양상을 나타내었다.

• Journal of Microbiology and Biotechnology
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• 제28권7호
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• pp.1141-1146
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• 2018

2'-Fucosyllactose (2'-FL) is one of the most important human milk oligosaccharides and has several health benefits for infants. The levels of 2'-FL in breast milk or samples from other sources can be quantified by high-performance liquid chromatography. However, this method cannot be used for simultaneous detection of the target compound in numerous samples. Here, we developed a simple method for quantifying 2'-FL in a microplate format. The method involves two steps: (i) release of $\text\tiny{L}$-fucose from 2'-FL by ${\alpha}$-(1-2,3,4,6)-$\text\tiny{L}$-fucosidase and (ii) measurement of NADPH formed during the oxidation of $\text\tiny{L}$-fucose by $\text\tiny{L}$-fucose dehydrogenase. This method enables measurement of up to 5 g/l 2'-FL in 50 min using a 96-well microplate. The efficiency and simplicity of the proposed method make it suitable for the analyses of a large number of samples simultaneously.

• 한국식품영양과학회지
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• 제30권4호
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• pp.697-702
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• 2001

본 연구는 한국산 겨우살이 물 추출물과 이에서 분리한 렉틴 성분을 실험적으로 간암을 유도한 흰쥐에게 투여하여 이들의 간 발암억제 효과를 조사하고자 하였다. 이를 위해 80~90 g정도로 이유된 3주령 Sprague-Dawley종을 유럽산겨우살이 추출물 투여(10 $\mu\textrm{g}$/kg BW, 100 $\mu\textrm{g}$/kg BW)와 렉틴(1 ng/kg Bw, 10 ng/kg BW)의 투여량 DEN 투여 여부에 따라 12군으로 나누어 발암물질 투여 1주일 후부터 주 2회 복강 투여하고 실험식이를 공급하였다. 총 사육기간은 9주간이었고 희생시킨후 혈청과 간 조직을 분리하여 조직병리학적 변화 측정을 위한 전암성 병변의 지표인 GST-P+ foci의 수와 면적을 측정하였고 생화학적 변화 측정을 위해 간 기능의 지표인 혈청 GOT와 GPT 활성도, 혈청 $\alpha$-L-fucosidase 활성도를 측정하였다. 그결과 다음과 같은 결론을 얻었다. (1)체중은 발암물질에 의해 식이 섭취가 감소하는 것을 고려하여 제한 섭취를 실시하였으나 발암물질 투여나 겨우살이 또는 렉틴의 투여에 따라 약간 감소하는 경향을 보였고 간 무게는 겨우살이 렉틴의 효과는 보이지 않으나 발암물질 투여에 따라 유의적으로 증가하였다. 체중에 대한 상대적인 간 무게에 대한 결과도 간 무게의 결과와 유사하였고 유럽산보다는 한국산 겨우살이 물 추출물의 간 무게와 체중에 대한 간 무게의 비율이 작게 나타나 한국산 겨우살이 물 추출물의 독성이 간에 영향을 작게 미치는 것으로 보여진다 (2) GST-P+ foci의 수나 면적은 겨우살이나 렉틴의 투여에 따라 유의적으로 감소하였고 유럽산과 한국산의 겨우살이 추출물 종류에 따라서는 한국산 겨우살이 추출물을 투여한 군에서 약간 증가하는 경향을 보였으나 한국산 겨우살이 물 추출물의 양을 증가시켜 100 $\mu\textrm{g}$/kg BW 수준으로 투여한 결과 foci의 수나 면적이 유의적으로 감소하여 가장 낮은 값을 보였다. 렉틴의 투여량에 따른 결과도 양이 증가함에 따라 GST-p_ foci의 수와 면적이 감소하였다. (3) 혈청 GOT 활성도는 유의적이지는 않지만 발암물질 투여에 따라 증가하는 경향을 보였고 겨우살이나 렉틴의 투여에 따라유의 으로 증가하였으며 겨우살이와 렉틴에 따라서는 GOT와 비슷한 경향을 나타냈다.을 나타냈다.

• Food Science and Biotechnology
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• 제18권6호
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• pp.1365-1370
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• 2009

Aspergillus kawachii extracellular pectinases were screened in liquid cultures with different carbon sources. The fungus grown on citrus pectin or lemon pomace produced at least one of these inducible pectinases: acidic polygalacturonase, pectin lyase, pectin methylesterase, $\alpha$-L-arabinofuranosidase, $\alpha$-1,5-endoarabinase, $\beta$-D-galactosidase/exogalactanase, and $\beta$-1,4-endogalactanase. The lemon-pomace filtrates also contained significant $\alpha$-L-rhamnosidase and $\beta$-D-fucosidase activities. Most of the screened pectinases were active at pH 2.0-2.5, indicating that the A. kawachii enzymes were acidophilic. Under the culture conditions employed we could not detect enzymatic degradation of soybean rhamnogalacturonan. The A. kawachii pectinase-production-related regulatory phenomena of induction-repression resemble those described for other Aspergillus sp.

• Reproductive and Developmental Biology
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• 제35권2호
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• pp.185-190
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• 2011

To evaluate the effect of spermatozoa culture on glycosidase activity of frozen-thawed spermatozoa in human, the spermatozoa were treated experimentally and assayed for activities of ${\alpha}$-L-fucosidase, ${\alpha}$-D-mannosidase, ${\beta}$-D-galactosidase and N-acetyl-${\beta}$-D-glucosaminidase (${\beta}$-GlcNAc'ase). The ${\beta}$-GlcNAc'ase activity was at least two-folds higher than other glycosidases regardless of spermatozoa incubation (p<0.05). The spermatozoa motility was decreased with incubation periods, but no effects by different glycosidases on the changes of spermatozoa motility during the various periods of incubation. In all glycosidases, the spermatozoa-zona binding rates in spermatozoa without incubation were higher than in spermatozoa incubated for 2 h (p<0.05). ${\beta}$-GlcNAc'ase is present mainly in the plasma membrane of spermatozoa frozen-thawed in human. It was also shown that the glycosidase activity was increased in all glycosidases in spite of lower sperm-zona binding by spermatozoa incubation.

• Biotechnology and Bioprocess Engineering:BBE
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• 제11권5호
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• pp.382-390
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• 2006

Various microorganisms were isolated from the surface waters and sediments of eutrophic lakes and reservoirs in Korea to enable an investigation of bacteria having algal lytic activities against Anabaena flos-aquae when water blooming occurs and to study enzyme profiles of algal lytic bacteria. Two bacterial strains, AFK-07 and AFK-13, were cultured, characterized and identified as Acinetobacter johnsonii and Sinorhizobium sp., respectively. The A. johnsonii AFK-07 exhibited a high level of degradatory activities against A. flos-aquae, and produced alginase, caseinase, lipase, fucodian hydrolase, and laminarinase. Moreover, many kinds of glycosidase, such as ${\beta}-galactosidase,\;{\beta}-glucosidase,\;{\beta}-glucosaminidase,\;and\; {\beta}-xylosidase$, which hydrolyzed ${\beta}-O-glycosidic$ bonds, were found in cell-free extracts of A. johnsonii AFK-07. Other glycosidases such as ${\alpha}-galactosidase,\;{\alpha}-N-Ac-galactosidase,\;{\alpha}-mannosidase,\; and\;{\alpha}-L-fucosidase$, which cleave ${\alpha}-O-glycosidic$ bonds, were not identified in AFK-07. In the Sinorhizobium sp. AFK-13, the enzymes alginase, amylase, proteinase (caseinase and gelatinase), carboxymethyl-cellulase (CMCase), laminarinase, and lipase were notable. No glycosidase was produced in the AFK-13 strain. Therefore, the enzyme system of A. johnsonii AFK-07 had a more complex mechanism in place to degrade the cyanobacteria cell walls than did the enzyme system of Sinorhizobium sp. AFK-13. The polysaccharides or the peptidoglycans of A. flos-aquae may be hydrolyzed and metabolized to a range of easily utilized monosaccharides or other low molecular weight organic substances by strain AFK-07 of. A. johnsonii, while the products of polysaccharide degradation or peptidoglycans were more likely to be utilized by Sinorhizobium sp. AFK-13. These bacterial interactions may offer an alternative effective approach to controlling the water choking effects of summer blooms affecting our lakes and reservoirs.