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http://dx.doi.org/10.4014/jmb.1803.03003

A Simple Enzymatic Method for Quantitation of 2'-Fucosyllactose  

Seydametova, Emine (Department of Integrative Biotechnology, College of Biotechnology and Bioengineering, Sungkyunkwan University)
Shin, Jonghyeok (Department of Integrative Biotechnology, College of Biotechnology and Bioengineering, Sungkyunkwan University)
Yu, Jiwon (Department of Integrative Biotechnology, College of Biotechnology and Bioengineering, Sungkyunkwan University)
Kweon, Dae-Hyuk (Department of Integrative Biotechnology, College of Biotechnology and Bioengineering, Sungkyunkwan University)
Publication Information
Journal of Microbiology and Biotechnology / v.28, no.7, 2018 , pp. 1141-1146 More about this Journal
Abstract
2'-Fucosyllactose (2'-FL) is one of the most important human milk oligosaccharides and has several health benefits for infants. The levels of 2'-FL in breast milk or samples from other sources can be quantified by high-performance liquid chromatography. However, this method cannot be used for simultaneous detection of the target compound in numerous samples. Here, we developed a simple method for quantifying 2'-FL in a microplate format. The method involves two steps: (i) release of $\text\tiny{L}$-fucose from 2'-FL by ${\alpha}$-(1-2,3,4,6)-$\text\tiny{L}$-fucosidase and (ii) measurement of NADPH formed during the oxidation of $\text\tiny{L}$-fucose by $\text\tiny{L}$-fucose dehydrogenase. This method enables measurement of up to 5 g/l 2'-FL in 50 min using a 96-well microplate. The efficiency and simplicity of the proposed method make it suitable for the analyses of a large number of samples simultaneously.
Keywords
2'-Fucosyllactose; quantitation; ${\alpha}$-(1-2,3,4,6)-$\text\tiny{L}$-fucosidase; $\text\tiny{L}$-fucose; $\text\tiny{L}$-fucose dehydrogenase;
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