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Cotransformation of Saccharomyces cerevisiae with Yip and Yep Vectors (YEp 및 YIp 벡터에 의(依)한 Saccharomyces cerevisiae의 Cotransformation)

  • Lee, Sung Bum;Rhee, In Koo
    • Current Research on Agriculture and Life Sciences
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    • v.4
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    • pp.36-41
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    • 1986
  • The transformation of Saccharomyces cerevisiae with YIp26, YRp7 and YEp13 was investigated. Transformation frequences of YIp5, YIp26, YRp7 and YEp13 in Escherichia coli HB101 was $5.1{\times}10^{-4}$, $1.5 {\times}10^{-3}$, $1.3{\times}10^{-3}$, $3{\times}10^{-3}$, respectively. When plasmids were used in covalently closed circular form, transformation frequency of YEp13 was $1.2{\times}10^{-4}$ in S. cerevisiae DBY747 and $3.3{\times}10^{-4}$ in S. cerevisiae MC16 and that of YRp7, YIp26 was $3{\times}10^{-6}$, below $6{\times}10^{-8}$ respectively in S. cerevisiae DBY747 by the method of Ito. Cotransformation of YIp26 and YEp13 in linear form increased the frequency of transformation with efficiences 270-fold higher than transformation of YIp26 only in S. cerevisiae DBY747. In cotransformation of YIp5+YEp13 and YIp26+YRp7 with S. cerevisiae DBY747 by Beggs' method. Expression frequency of YIp5+YEp13 and YIp26+YRp7 was $4{\times}10^{-6}$, $1.5{\times}10^{-6}$, respectively. The recombinant plasmid of cotransformant was thought that YIp26 and YEp13, YIp5 and YEp13, and YIp26 and YRp 7 were ligated in vivo in S. cerevisiae DBY747.

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Fast and Efficient Macroblock Mode Decision Algorithm in H.264/AVC (H.264/AVC 고속의 효율적인 매크로블록 모드 결정 알고리즘)

  • Park, Seong-Bin;Kim, Yong-Kwan
    • Journal of the Institute of Electronics Engineers of Korea SP
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    • v.48 no.3
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    • pp.42-49
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    • 2011
  • In this paper, we propose a fast macroblock mode decision algorithm in H.264/AVC, based on the image sequence statistics. Specically, considering the directional characteristics of image sequences, we eliminate sub$8{\times}4$ or sub$4{\times}8$ mode decision process based on the rate-distortion cost of Inter$16{\times}8$ or Inter$8{\times}16$ mode respectively. Additionally, exploiting the optimal modes of submacroblock in inter$8{\times}8$ mode, we propose an algorithm to eliminate Intra$4{\times}4$ or Intra$16{\times}16$ mode decision process selectively. From the simulation results, the proposed method reduce the encoding time by maximum 70% of total, compared with the other conventional methods.

Exploring natural hybridizations among Asplenium ruprechtii and related taxa in Korea

  • LEE, Chang Shook;YEAU, Sung Hee;CHUNG, Kyong-Sook
    • Korean Journal of Plant Taxonomy
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    • v.49 no.2
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    • pp.127-139
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    • 2019
  • The purported four hybrid origins of Asplenium in Korea were tested based on morphological, cytological and DNA sequence data. Asplenium castaneo-viride, A. ${\times}$ uiryeongse, A. ${\times}$ montanus, and A. ${\times}$ kitazawae share several morphological characteristics with the Asian walking fern A. ruprechtii and related taxa as parents and show a sympatric distribution with the putative parents, raising the possibility of hybrid origins: A. castaneo-viride (A. ruprechtii and A. incisum), A. ${\times}$ uiryeongse (A. ruprechtii and A. pekinense), A. ${\times}$ montanus (A. ruprechtii, A. trichomanes, and A. incisum), and A. ${\times}$ kitazawae (A. ruprechtii and A. sarelii). We investigated flow cytometry and chloroplast DNA sequence data (rbcL, rps4-trnS, and rps4-trnS intergenic spacer) to clarify the hybridization and origin of each hybrid. In the flow cytometry analyses, A. ruprechtii shows diploid (2x) only, whereas A. castaneo-viride (3x, 4x), A. ${\times}$ uiryeongse (3x), A. ${\times}$ montanus (3x, 4x), and A. ${\times}$ kitazawae (2x, 4x) exhibit polyploidy, suggesting hybrid events along speciation. The rbcL and rps4-trnS and rps4-trnS intergenic spacer data suggest that A. ruprechtii is one the maternal ancestors of all four hybrids. In addition, the rps4-trnS and rps4-trnS intergenic spacer data indicate that A. incisum is also the maternal ancestor of A. ${\times}$ kitazawae and A. ${\times}$ montanus, proposing multiple hybridization events for these two hybrids. In A. ${\times}$ montanus, morphological features such as the leaf forms and sympatric distributions of the species also support the multimaternal hypothesis, but the morphological features of A. ${\times}$ kitazawae must be examined with consideration of hybrid events. To clarify the complex hybrid evolutionary lineages of the four Asplenium hybrids, further research with taxon sampling and molecular markers should be conducted.

Usability Evaluation of the Size of Small Touch Keys for the Smart Phone (스마트 폰 터치스크린에서의 작은 터치 키의 사용성 연구)

  • Kim, Bo-Ram;Kim, Tae-Il;Lim, Young-Jae;Jung, Eui-S.
    • Journal of Korean Institute of Industrial Engineers
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    • v.38 no.2
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    • pp.80-88
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    • 2012
  • This paper focuses on small touch key size and spacing that affect the usability of the smart phones. The experiments examined thirty-six different touch key designs, combinations of four touch key sizes (width (mm) ${\times}$height (mm); $4{\times}4$, $4{\times}7$, $7{\times}4$, and $7{\times}7$), and nine spacings (horizontal (mm)${\times}$vertical (mm); $0{\times}0$, $0{\times}1$, $0{\times}3$, $1{\times}0$, $1{\times}1$, $1{\times}3$, $3{\times}0$, $3{\times}1$, and $3{\times}3$). Forty participants volunteered in the experiment. The completion time, error rate, identification discomfort, and control discomfort were measured. The results revealed that the touch key sizes of $7{\times}7$ and $7{\times}4$ provide the best performance in terms of the error rate measure, while the touch key size of $7{\times}7$ provides the best results for other measures. In addition, touch key spacings of $1{\times}1$, $1{\times}3$ and $3{\times}1$ are regarded as the best for all the measures. The results of this study suggest a practical approach for the small touch key design for the smart phones.

A Study on High Reynolds Number Flow in Two-Dimensional Closed Cavity (2차원 밀폐 캐비티의 고레이놀즈수 흐름에 관한 연구)

  • 최민선;송치성;이영호
    • Journal of Advanced Marine Engineering and Technology
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    • v.20 no.3
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    • pp.101-109
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    • 1996
  • Two-dimensional lid-driven closed flows within square cavity were studied numerically for four Reynolds numbers : $10^4$, 3$\times10^4$, 5$\times10^4$ and 7.5$\times10^4$. A convective difference scheme to maintain the same spatial accurary by irregular grid correction is adopted by applying the interior division principle. Grid number is $80\times80$and its minimum size is about 1/400 of the cavity height. At Re=$10^4$, periodic migration of small eddies appearing in corner separation region and its temporal sinusoidal fluctuation are represented. At three higher Reynolds numbers(3$\times10^4$, 5$\times10^4$ and 7.5$\times10^4$), an organizing structure of four consecutive vorticles at two lower corners is revealed from time-mean flow patterns. But, instantaneous flow characteristics show very random unsteady fluctuation mainly due to the interaction between rotating shed vortices and stationary eddies within the corners.

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A Study on Unsteady Flow Characteristics of Closed Cavity with Obstacle (장애물을 갖는 밀폐공간의 비정상 유동특성에 관한연구)

  • 조대환
    • Journal of Advanced Marine Engineering and Technology
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    • v.23 no.2
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    • pp.176-183
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    • 1999
  • This paper is aimed to investigate behaviour of vortex in 2-D step cavity with high Reynolds numbers$(3.2{\times}10^{3},\;10^{4},\;3{\times}10^{4},\;5{\times}10^{4}\;and\;7{\times}10^{4})$. The SOLA algorithm which is MAC type was adopted to solution method computing the flow field on irregular grid. In case of $Re=7{\times}10^{4}$ flow behavior is steady bu periodic unsteady sinusoidal fluctuation of local velocity and kinetic energy is found for $Re=10^{4}$ Continuous movements of small eddies in the secondary flow regions are discov-ered for $3{\times}10^{4}$ Generation of eddies and their active migrating behavior are detected over $Re=5{\times}10^{4}$ resulting in complete unsteady and non-linear flow characteristics Furthermore a typhoon-like vortex(TLV) appears intermittently and rotates along the separation regions and boundary layers.

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건어물의 위해분석과 건어물을 이용한 조리음식의 안전성 확보를 위한 전처리 적용 방안

  • 오세인;이재학;배현주
    • Proceedings of the Korean Society of Food and Cookery Science Conference
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    • 2003.05a
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    • pp.101-101
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    • 2003
  • 우리나라 식단에서 예로부터 널리 이용되어 온 건어물 중 서울시내 급식소 12곳에 입고 된 쥐어채, 오징어채, 북어채를 대상으로 미생물학적 위해분석을 실시하여 원재료의 오염실태를 조사한 후 최종조리음식의 안전성을 확보하기 위해 조리공정 전에 적용할 수 있는 세척, 볶기, 데치기, 전자레인지 가열 둥의 전처리 과정을 적용하여 효과적으로 위해를 제거할 수 있는 방법을 모색한 결과 일반세균의 경우 취어채는 1.2$\times$$10^3$~8.2$\times$$10^{6}$ CFU/g, 오징어채는 2.2$\times$$10^4$~3.3$\times$$10^{8}$ CFU/g, 북어체는 4.0$\times$$10^2$~1.7$\times$$10^{6}$ CFU/g 범위로 검출되었고, 대장균군의 경우 쥐어채는 1.0$\times$$10^3$~3.4$\times$$10^4$ CFU/g 범위로, 오징어채는 $10^1$ CFU/g 이하에서 1.4$\times$$10^4$ CFU/g 까지, 북어채는 $10^1$ CFU/g 이하에서 4.5$\times$$10^3$ CFU/g 까지 검출되어 전체적으로 위생상태가 불량한 경우가 많은 것으로 평가되었다. (중략)

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Physical Parameters of Late Type Spiral Galaxies I-Mass and Luminosity of NGC 6946

  • Kim, Sug-Whan;Chun, Mun-Suk
    • Journal of Astronomy and Space Sciences
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    • v.2 no.2
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    • pp.101-115
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    • 1985
  • Using Brandt model the mess distribution of the late type spiral galaxy NGC 6946 was derived, and the total mass was reestimated to understand the M/L ratio of this galaxy. Two kinds of the rotation curve with shape parameter n=1 and 3.3 were examined. The following are the main results; (1) The total masses of NGC 6946 are $3.1\times$$10^{11}$ M(n=1) AND $2.8\times$$10^{11}$ M(n=3.3) respectively. and the corresponding M/L are about 17 and 16 for both cases. (2) The optical image in the blue light, whose radius is 9.6 kpc, has 8$\times$$10^{10}$ M and 1.4$\times$$10^{11}$ M. These give the value of M/L about 5 and 8 respectively. (3) The masses and M/L of the nuclear region within 1.2 kpc are 4.0$\times$$10^{9}$ M 4.7$\times$$10^9$ M and 3, 4 for both cases. Those of the disk from 1.2 kpc to 9.6 kpc are 7.6$\times$1$10^{10}$M, 1.4$\times$$10^{11}$M, and 5, 8. (4) The masses of the outer halo extended to few hundreds kiloparsecs are 2.3$\times$$10^{11}$ M and 1.4$\times$$10^{11}$M. The corresponding M/L are about 62 and 37.

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Syntheses and Surface Active Properties of Amphoteric Surfactant Derivatives(5) - Basic Properties of Derivatives from Imidazoline (양쪽성 계면활성제의 유도체합성 및 계면성에 관한 연구(제5보) - 이미다졸린으로부터 유도된 유도체의 기초적 물성 -)

  • Ro, Y.C.;Kim, T.Y.;Jeong, J.K.;Nam, K.D.
    • Applied Chemistry for Engineering
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    • v.7 no.2
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    • pp.215-220
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    • 1996
  • Nine kinds of amphoteric surfactants were derived from 1-(2-hydroxyethyl)-2-undecyl-2-imidazoline. Their surface activities including surface tension and isoelectric points were measured in aqueous solution and critical micelle concentration(cmc) was also evaluated by the measurement of surface tension. From the measurement of surface tension, carboxylated amides revealed to be 26~40dyne/cm at $4.0{\times}10^{-4}{\sim}1.0{\times}10^{-3}mol/{\ell}$, sulfonated or sulfated imidazolines, 30~35 dyne/cm at $1.5{\times}10^{-3}{\sim}2.5{\times}10^{-3}mol/{\ell}$ and sulfonated amides, 25~33 dyne/cm at $5.8{\times}10^{-4}{\sim}8.0{\times}10^{-4}mol/{\ell}$ concentration range. It was found that isoelectric points of carboxylated amides were pH 7.2~7.9 and those of sulfonated or sulfated imidazolines and sulfonated amides were pH 4.5~5.5.

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Calculating an inverse of a $4{\times}4$ matrix using Neon (Neon 을 사용한 $4{\times}4$ 행렬의 역행렬 연산)

  • Oh, Yu-Yeon;Lee, Chang-Gun
    • Proceedings of the Korean Information Science Society Conference
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    • 2012.06c
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    • pp.344-346
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    • 2012
  • 스마트폰에서의 3D 게임/애플리케이션에 대한 사용자의 요구는 나날이 증가하고 있다. 3D 게임/애플리케이션은 내부적으로 $4{\times}4$ 행렬을 가지고 여러 가지 좌표 변환을 수행하기 때문에, 보다 빠른 3D 그래픽스 처리를 위해서는 $4{\times}4$ 행렬 연산의 최적화가 필수적이다. $4{\times}4$ 행렬 연산중에 역행렬 연산에 대해 살펴보고, ARM 프로세서에서 지원하는 Neon 연산자를 이용해서 $4{\times}4$ 행렬의 역행렬 연산을 개선할 수 있다.