• Title/Summary/Keyword: $(P.S.)^*_c$ condition

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Optimization of Culture Condition and Media Composition on the Production of Cordycepin by Cordyceps militaris. (Cordycepin 생성을 위한 배양조건 및 배지조성의 최적화)

  • Jo Sung-Jun;Lee Tae-Hee;Chae Dae-Hoon;Han Yeong-Hwan
    • Korean Journal of Microbiology
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    • v.40 no.3
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    • pp.217-220
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    • 2004
  • The effect of media composition and culture condition on mycelial growth and cordycepin (3'-deoxyadenosine) production was determined using Cordyceps spp. Among the strains of C. militaris and C. sinensis tested, C. militaris KCTC 6862, C. militaris DGUM 32003 and C. militaris KCTC 16932 were excellent for the production of cordycepin. The optimal temperature and pH for production of cordycepin were $24^{\circ}C$ and pH ranged from 6.0 to 10, respectively. Among various sources of carbon and nitrogen tested, glucose and tryptone were very excellent for the production of cordycepin, respectively. After 5days cultivation with 1% of tryptone with nitrogen source, 39mg/l of cordycepin was produced. However, addition of phosphorus sources reduced the production of cordycepin.

Characteristics of Culture for Emulsive Biosurfactant-Strain from the Soil (토양으로부터 분리한 유화성 생체계면활성 균주의 배양 특성)

  • 임윤택;윤용수
    • Journal of environmental and Sanitary engineering
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    • v.11 no.3
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    • pp.69-77
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    • 1996
  • The result of isolated and selected to the strain having the emulsifying activity from soil's strain the strain was identified as Candida genus. The strain was investigated with culture condition at pH culture temperature, flow rate of air, strring rate etc., and physicochemical properties of the biosurfactant were examined. The optimum composition of medium for a strain cultivation were obtained as follow : glucose ; 100g/L, yeast extract ; 10g/L, urea ; 1.0g/L, KH$_{2}$PO$_{4}$ ; 50mg/L, MgSO$_{4}$ ; 500mg/L, and the op condition of cultivation was as follow : pH ; 3.0, temperatlue ; 24$\circ $C, strring rate ; 40rpm. The maximum yield of biosurfactant was obtained by pH ; 3.0-3.5, and temperature ; 25$\circ $C. The degree of emulsification of syntesized biosurfactant was increased clearly by increasing concentration of biosurfactant and it's stability was maintained for a long time. The surface tension of biosurfactant was varied with pH, especially it was showed that the surface tension was high at acidic pH.

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Characteristics of Bacillus sphaericus PSB-13 as Phosphate Solublizing Bacterium Isolated from Citrus Orchard Soil (감귤원 토양에서 분리한 인산염 가용화 미생물 Bacillus sphaericus PSB-13의 특성)

  • Joa, Jae-Ho;Lim, Han-Cheol;Han, Seung-Gap;Chun, Seung-Joung;Suh, Jang-Sun
    • Korean Journal of Soil Science and Fertilizer
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    • v.40 no.5
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    • pp.405-411
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    • 2007
  • This study was carried out to measure insoluble phosphorus fractions content fixed in different soil type and isolate a superior phosphate solublizing bacteria(PSB) producing free phosphate in citrus orchard soil. Distribution of insoluble phosphate fraction ordered Al-P>Ca-P>Fe-P in the investigated citrus orchards. Insoluble phosphate fraction such as Al-P, Ca-P, Fe-P were higher in volcanic ash than in non-volcanic ash soil. A PSB with high holo zone in PDA-P medium isolated from citrus orchard soil. This strain identificated by MIDI system as Bacillus sphaericus. The optimum growth of pH and temperature were at 4~5, $30^{\circ}C$, respectively. When Bacillus sphaericus cultured at $25^{\circ}C$, 150 rpm condition in LB broth medium included different phosphate. Bacillus sphaericus produced free phosphate in the culture broth medium from tricalcium-phosphate(207.0 ppm), aluminium phosphate(324.5 ppm) and hydroxyapatite(334.8 ppm) and Phosphatase activity of Bacillus sphaericus was higher at $35^{\circ}C$ culture condition than that of $25^{\circ}C$. Two type preparation inoculated Bacillus sphaericus made with carrier materials such as Bentonite, $CaCO_3$, Sodium alginate. Density of PSB in this preparation conserved at $10^5c.f.u.\;g^{-1}$ level during storage in different temperature condition for 7 month. It also showed that free phosphate produced at PDA-P medium.

A NOTE ON STRONG REDUCEDNESS IN NEAR-RINGS

  • Cho, Yong-Uk
    • The Pure and Applied Mathematics
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    • v.10 no.4
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    • pp.199-206
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    • 2003
  • Let N be a right near-ring. N is said to be strongly reduced if, for $a\inN$, $a^2 \in N_{c}$ implies $a\;\in\;N_{c}$, or equivalently, for $a\inN$ and any positive integer n, $a^{n} \in N_{c}$ implies $a\;\in\;N_{c}$, where $N_{c}$ denotes the constant part of N. We will show that strong reducedness is equivalent to condition (ⅱ) of Reddy and Murty's property $(^{\ast})$ (cf. [Reddy & Murty: On strongly regular near-rings. Proc. Edinburgh Math. Soc. (2) 27 (1984), no. 1, 61-64]), and that condition (ⅰ) of Reddy and Murty's property $(^{\ast})$ follows from strong reducedness. Also, we will investigate some characterizations of strongly reduced near-rings and their properties. Using strong reducedness, we characterize left strongly regular near-rings and ($P_{0}$)-near-rings.

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LIMIT RELATIVE CATEGORY THEORY APPLIED TO THE CRITICAL POINT THEORY

  • Jung, Tack-Sun;Choi, Q-Heung
    • Bulletin of the Korean Mathematical Society
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    • v.46 no.2
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    • pp.311-319
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    • 2009
  • Let H be a Hilbert space which is the direct sum of five closed subspaces $X_0,\;X_1,\;X_2,\;X_3$ and $X_4$ with $X_1,\;X_2,\;X_3$ of finite dimension. Let J be a $C^{1,1}$ functional defined on H with J(0) = 0. We show the existence of at least four nontrivial critical points when the sublevels of J (the torus with three holes and sphere) link and the functional J satisfies sup-inf variational inequality on the linking subspaces, and the functional J satisfies $(P.S.)^*_c$ condition and $f|X_0{\otimes}X_4$ has no critical point with level c. For the proof of main theorem we use the nonsmooth version of the classical deformation lemma and the limit relative category theory.

Cultural Condition of Pseudomonas mendocina for Polysaccharide Production (Pseudomonas mendocina 에 의한 Polysaccharide 생산)

  • Yoo, Jin-Young;Chung, Dong-Hyo
    • Korean Journal of Food Science and Technology
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    • v.21 no.5
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    • pp.619-623
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    • 1989
  • The cultural condition of Pseudomonas mendocina for polysaccharide production was examined. The optimal medium contains following composition per liter of distilled water: Sucrose 23.75g, $(NH_4)_2SO_4$ 1.57g, Yeast extract 0.5g, $KH_2PO_4\;2.9g,\;MgSO_4.\;7H_2O\;1.0g,\;CaCO_3$ 2.5g. The optimum temperature and pH were $30^{\circ}C$ and 6.5. At the condition. Ps mendocina produced 5.98g/l of polysaccharide. The culture viscosity after 3 days was 191mPa.s at $70sec^{-1}$. The product yield $(Y_{p/s})$ and specific productivity $(Q_p)$ were 25.18% and 32.83mg/g-cell/h.

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Effect of Plating Condition and Plating Rate on the Magnetic Properties of Electroless Ni-Cu-P Deposits (무전해 Ni-Cu-P 도금층의 자성에 미치는 도금조건과 도금속도의 영향)

  • Oh, I.S.;Lee, T.H.
    • Journal of Power System Engineering
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    • v.10 no.3
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    • pp.58-66
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    • 2006
  • The effect of bath composition, plating condition and plating rate on the magnetic properties of electroless Ni-Cu-P deposits were investigated. With increasing $CuSO_4$ concentration in the bath, plating rate increased, while the Br value of deposits decreased Sharply. Plating rate increased up to 34% with the addition of 200ppm of NaF and 0.8ppm of Thiourea to the bath. Plating reaction had been ceased by the increase of pH above 11.3, bath temperature higher than $90^{\circ}C$ and under $70^{\circ}C$. The Br value of deposit was uniform with various concentration of complexing agent (Sodium citrate, Ethylenediamine) in the bath. The Br value of deposit was almost equal to that found by the addition of stabilizer(Thiourea) and accelerator(NaF). The Br value of deposit was uniform in plating time(120 min) and heat treatment temperature(below $200^{\circ}C$), and were confirmed to have adequate bath stability for practical use.

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Vitamin C and antioxidant capacity stability in cherry and romaine during storage at different temperatures (저장 조건에 따른 로메인 및 체리의 비타민 C 함량 및 항산화능의 변화)

  • Park, Hee Jung;Lee, Myung Joo;Lee, Hye Ran
    • Journal of Nutrition and Health
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    • v.49 no.1
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    • pp.51-58
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    • 2016
  • Purpose: The aim of this work was to study the change in antioxidant activity depending on storage temperature and storage period in romaine and cherry. Method: The plant material was stored at $0.7{\pm}0.6^{\circ}C$, $3.5{\pm}2.8^{\circ}C$, and $4.7{\pm}1.4^{\circ}C$. Cherry and romaine were stored for a period of 9 days and 7 days, respectively. The cherry was taken from each group of samples at regular intervals of days and the romaine was taken from each group of samples at regular intervals of 2days. Vitamin C, total polyphenol, and total flavonoid stability and antioxidant capacity including DPPH, total antioxidant capacity (TAC) were measured. Results: For cherry, the levels of TAC and flavonoid were higher at the $0.7{\pm}0.6^{\circ}C$ condition than other conditions (p < 0.05). The polyphenol and vitamin C levels were not significantly different among storage conditions. In the case of romaine, the level of TAC was highly preserved until 7 days at the $0.7{\pm}0.6^{\circ}C$ condition. Vitamin C level was significantly lower at the $3.5{\pm}2.8^{\circ}C$ condition (p < 0.05). DPPH activity was highest at the $0.7{\pm}0.6^{\circ}C$ condition (p < 0.05). DPPH activity was shown in order of $0.7{\pm}0.6^{\circ}C>4.7{\pm}1.4^{\circ}C>3.5{\pm}2.8^{\circ}C$. Conclusion: The results indicated that the narrow differences and fluctuation in temperature were associated with antioxidant capacity and it might enhance the nutritional shelf life of vegetables and fruits.

Estimation of Ammonia Stripping Condition for Adequate Aerobic Liquid-Composting of Swine Manure (돈분뇨의 적합한 호기성 액비화를 위한 암모니아 탈기조건 설정)

  • Son, Bo-Kyoon;Gang, Seong-Gu;Jo, Eun-Ju;Kim, Shin-Do;Lee, Chang-Ju;Kim, Jeong-Ho
    • Korean Journal of Soil Science and Fertilizer
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    • v.39 no.2
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    • pp.73-79
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    • 2006
  • Aeration is the most important and indispensable operation unit for the treatment of swine manure using aerobic liquid-composting process. The composting of swine manure depends on biological treatment process, but the highly concentrated ammonia nitrogen is required a pretreatment to expect the appropriate efficiency of the biological treatment process. In this study, pilot experiments have been carried out to estimate of the fit condition about ammonia stripping process as a pretreatment to aerobic liquid- composting. pH adjustment with $Ca(OH)_2$ was economically superior to use of NaOH and optimum pH of ammonia stripping was 12.3, ammonia nitorgen was rapidly removed as pH were increased at $$35^{\circ}C$$. When air stripping is performed before aerobic liquid-stripping, a high initial pH is required for complete ammonia removal and is additional effects such as organic substances, phosphorus, turbidity, and color removal. Stripping process was very efficient in the pretreatment of highly concentrated ammonia nitrogen for composting of swine manure. Emission rate of gaseous ammonia was $0.5355mole\;s^{-1}$ at initial time and $0.0253mole\;s^{-1}$ at finitial time. The fit condition of ammonia stripping in this study were at the temperature of $$35^{\circ}C$$, and the pH of 12.3 during 48 hours.

The Optimal Enrichment Condition of Rotifer Brachionus rotundiformis (소화효소 활성으로 본 rotifer Brachionus rotundiformis의 적정 영양강화 조건)

  • Kwon, O-Nam;Park, Heum-Gi
    • Journal of Aquaculture
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    • v.21 no.1
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    • pp.41-46
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    • 2008
  • The purpose of the study was to suggest the optimal lipid enrichment conditions used digestive enzyme activity of rotifer changing due to water temperature and salinity. The high population growth appeared at the experiment temperature more than 28 degrees highly on the culture temperature(maximum 32 degrees, 1,453 individual/mL). The fecundity was low at high temperature, and the egg ratio was high at low temperature. Population growth of 10 and 15 ppt appeared in most highly, but the fecundity and the egg ratio were high most significantly appeared in natural seawater(32 psu). The digestive enzyme activity by the culture environment mainly showed high activity in natural seawater(amylase exclusion, 15 psu). However, the TAP activity by the water temperature showed highly at the more high temperature, but the amylase and the lipase appeared at low temperature. We carried out the lipid enrichment at 20 degrees and 26 degrees in a condition of the natural seawater. Total protein, the total essential amino acids differed not significantly. The methionine content that was essential amino acids, a total lipid content, unsaturated index of fatty acids, DHA and the DHA/EPA ratio were high significantly each in $20^{\circ}C$ enrichment trial. Therefore, we could suggest the $20^{\circ}C$ and natural seawater for the optimal lipid enrichment condition in aquaculture, because methionine contents, several indexes by the lipid, TG-lipase activity, fecundity and egg ratio are high.