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Optimization of Culture Condition and Media Composition on the Production of Cordycepin by Cordyceps militaris.  

Jo Sung-Jun (동국대학교 생물학과)
Lee Tae-Hee (동국대학교 생물학과)
Chae Dae-Hoon (동서제약㈜)
Han Yeong-Hwan (동국대학교 생명과학과)
Publication Information
Korean Journal of Microbiology / v.40, no.3, 2004 , pp. 217-220 More about this Journal
Abstract
The effect of media composition and culture condition on mycelial growth and cordycepin (3'-deoxyadenosine) production was determined using Cordyceps spp. Among the strains of C. militaris and C. sinensis tested, C. militaris KCTC 6862, C. militaris DGUM 32003 and C. militaris KCTC 16932 were excellent for the production of cordycepin. The optimal temperature and pH for production of cordycepin were $24^{\circ}C$ and pH ranged from 6.0 to 10, respectively. Among various sources of carbon and nitrogen tested, glucose and tryptone were very excellent for the production of cordycepin, respectively. After 5days cultivation with 1% of tryptone with nitrogen source, 39mg/l of cordycepin was produced. However, addition of phosphorus sources reduced the production of cordycepin.
Keywords
cordycepin; Cordyceps militaris; culture condition; tryptone;
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