Background: This study examined the changes in the leg muscle activities of 30 healthy subjects according to different pressures caused by isometric hip adduction during squat exercise. Methods: With their knee flexed at 60°, the subjects performed isometric hip adduction with a pressure of 0, 20, 40, and 60mmHg. Surface electromyography was used to measure their muscle activities. Changes in the activities of the muscles, such as the gluteus maximus (GMAX), Gluteus medius (GM), vastus medialis oblique (VMO), vastus lateralis oblique (VLO), biceps femoris (BF), and Tensor fasciae latae (TFL) muscles, were analyzed. Results: The activities of the VMO, VLO, GM, BF, and GMAX muscles were significantly different after exercise compared to that before exercise (p<.05). The activities of all the leg muscles measured were highest at a pressure of 60mmHg during isometric hip joint adduction. Conclusion: The pressure produced by isometric hip joint adduction during squat exercise increased the leg muscle activities of the subjects. These results will provide basic data on effective squat exercise to alleviate knee joint diseases.