The purpose of this study was to investigate the effects of safety culture on the safety awareness and safety behaviors of manufacturing workers, and to suggest ways for manufacturing workers to understand the safety culture and improve safety awareness and safety behavior. In order to verify this, data were collected from 282 research subjects for 1 month from August 1 to 31, 2018, using the manufacturing workers in the Seoul and Gyeonggi area as a population. The results of the study are as follows. First, the safety culture (safety climate, safety procedures) has a positive effect on the safety awareness (recognition of importance, interest and participation inducement) of the manufacturing workers. Second, the safety culture (safety climate, safety procedures) has a positive effect on the safety behavior (safety planning, safety check) of manufacturing workers. Third, safety awareness (recognition of importance, interest and inducement of participation) has a positive influence on the safety behavior (safety planning, safety check) of manufacturing workers. Fourth, the safety awareness (recognition of importance, interest and inducement of participation) appears to have a partial mediating effect in relation to safety culture (safety climate, safety procedure) and safety behavior (safety planning, safety check) of manufacturing workers. The implication of this study is that although the industrial accidents have occurred in the manufacturing industry in recent years, the studies on the workers in the manufacturing industry are insufficient. However, this study is meaningful that it has suggested ways for manufacturing workers to understand the safety culture and improve the safety awareness and safety behavior by analyzing the effects of safety culture on safety awareness and safety behavior of manufacturing workers.