Objectives : We performed this study to search the best time for HRV measuring and which index has the validity in a short term measuring. Methods : At first, three examiner measured electrocardiogram(ECG) of 5 people(4 male + 1 female) for 20 minutes in two times. and we devided ten regions (0-5, 5-10, 10-15, 15-20, 0-10, 0-15, 0-20, 5-15, 10-20, 5-20 minutes). After that we finded the HRV index for each regions. To evaluate the validity, we used Gage Repeatability and Reproducibility Study by MINITAB(ver. 13.20). If the '%Study Var' is under 30 and 'Number of Distinct Categories' is over 4, we could tell it has validity(repeatability and reproducibility). Results : 1) The region and HRV index has repeatability and reproducibility, was HF index in 10-15 minutes. The HF index in 15-20 minutes gained 27.41(%Study Var) and 5(Number of Distinct Categories). 2) The HF index in 10-15 minutes has the better reproducibility than repeatability. The value of measurement is under the control. There is consistency among the examiner. The distribution of measurement is not large. There is no interaction between examiner and each people. 3) The HF index in 10-15, 0-10, 0-15, 0-20, 10-20 minutes was not enough to gain the repeatability and reproducibility. But it had some meanings ; %Study Var, Number of Distinct Categories is 32.68, 4 for 10-15 minutes, 34.43, 4 for 0-10 minutes, 34.44, 4 for 0-15 minutes, 33.56, 4 for 0-20 minutes, 32.07, 4 for 10-20 minutes. Conclusions : This study shows the best index of measuring HRV is the HF index in 15-20 minutes. So, from now on we should measure the HRV for 20 minutes and extract the HF index in 15-20 minutes. It must be the standard for HRV measuring.