Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Flavonoids from the arial parts of Artemisia agryi and their antioxidant capacity through GSH recovery effect

황해쑥(Artemisia agryi)으로부터 flavonoid 화합물들의 분리 동정과 세포 내 GSH 회복능을 통한 항산화 활성 평가

  • Hyeon Seon, Na (Graduate School of Biotechnology and Department of Oriental Medicinal Biotechnology, Kyung Hee University) ;
  • Dahye, Yoon (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA) ;
  • Hyeong-Ju, Jeon (Department of Food & Nutrition, Jangan University) ;
  • Dae Young, Lee (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA) ;
  • Hyoung-Geun, Kim (Graduate School of Biotechnology and Department of Oriental Medicinal Biotechnology, Kyung Hee University)
  • Received : 2022.09.27
  • Accepted : 2022.10.04
  • Published : 2022.12.31
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Abstract

The arial parts of Artemisia argyi were extracted with methanol : water (70:30), and the concentrates was partitioned into EtOAc (ethyl acetate), n-BuOH (normal butanol), and H2O (water) fractions. The repeated silica gel and ODS (octadecyl silica gel) column chromatographies for EtOAc and n-BuOH fractions led to isolation of four flavonoids without any ambiguity based on intensive interpretation of several spectroscopic data including nuclear magnetic resonance, and mass spectrometry. The chemical structure of the isolated compounds revealed to (2S)-naringenin (1), 3-methylkaempferol (2), 3,3'-dimethylquercetin (3), and 3,3',4'-trimethylquercetin (4). These four compounds were first isolated from A. argyi through this study. In this study, four compounds isolated from A. argyi showed an increase in glutathione mean and a decrease in glutathione heterogeneity so that the compounds uniformly raised the intracellular glutathione (GSH) level. Based on these results, it is considered that it can be used as a functional pharmacological material.

황해쑥을 MeOH : H2O =70:30 용매로 추출하고, 얻어진 추출물을 EtOAc n-BuOH 및 water로 용매 분획 하였다. 이 중 n-BuOH 분획으로부터 silica gel (SiO2)과 octadecyl silica gel (ODS) column chromatography로 정제하여 4종의 화합물을 분리하였다. Nuclear magnetic resonance 및 mass spectrometry 등의 스펙트럼 데이터를 통해 화합물의 화학구조를 (2S)-naringenin (1), 3-methylkaempferol (2), 3,3'-dimethylquercetin (3), and 3,3',4'-trimethylquercetin (4) 로 구조 동정하였다. 분리한 화합물 4종은 황해쑥에서는 처음 분리동정 되었다. 본 연구를 통해 glutathione mean의 증가와 glutathione heterogeneity 감소를 보인 황해쑥 유래 화합물 1-4가 세포내 글루타티온 (GSH) 수준을 균일하게 상승시키는 효능이 있음을 확인함으로써 항산화능을 확인하였다. 이러한 결과를 바탕으로 황해쑥의 기능성 소재로 활용가능성을 확인하였다.

Keywords

Acknowledgement

본 성과물은 농촌진흥청 연구사업(세부과제번호: PJ01420403)의 지원에 의해 이루어진 것입니다. 지원에 감사드립니다.

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