Browse > Article
http://dx.doi.org/10.3839/jabc.2022.032

Flavonoids from the arial parts of Artemisia agryi and their antioxidant capacity through GSH recovery effect  

Hyeon Seon Na (Graduate School of Biotechnology and Department of Oriental Medicinal Biotechnology, Kyung Hee University)
Dahye Yoon (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA)
Hyeong-Ju Jeon (Department of Food & Nutrition, Jangan University)
Dae Young Lee (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA)
Hyoung-Geun Kim (Graduate School of Biotechnology and Department of Oriental Medicinal Biotechnology, Kyung Hee University)
Publication Information
Journal of Applied Biological Chemistry / v.65, no.4, 2022 , pp. 247-252 More about this Journal
Abstract
The arial parts of Artemisia argyi were extracted with methanol : water (70:30), and the concentrates was partitioned into EtOAc (ethyl acetate), n-BuOH (normal butanol), and H2O (water) fractions. The repeated silica gel and ODS (octadecyl silica gel) column chromatographies for EtOAc and n-BuOH fractions led to isolation of four flavonoids without any ambiguity based on intensive interpretation of several spectroscopic data including nuclear magnetic resonance, and mass spectrometry. The chemical structure of the isolated compounds revealed to (2S)-naringenin (1), 3-methylkaempferol (2), 3,3'-dimethylquercetin (3), and 3,3',4'-trimethylquercetin (4). These four compounds were first isolated from A. argyi through this study. In this study, four compounds isolated from A. argyi showed an increase in glutathione mean and a decrease in glutathione heterogeneity so that the compounds uniformly raised the intracellular glutathione (GSH) level. Based on these results, it is considered that it can be used as a functional pharmacological material.
Keywords
Artemisia argyi; Flavonoid; Glutathione recovery; Nuclear magnetic resonance;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Lee JH, Kim YJ, Lee YJ, Jeong JT, Park CG, Chang JK (2018) Crop characteristics and flavonoid contents of Mugwort (Artemisia argyi H.Lev. & Vaniot). Horticultural Science and Technology 31: 647-657. doi: 10.12972/kjhst.20180065    DOI
2 Jung BS, Shin MG (1990) Hyangyak dictionary. Yeongrimsa Press, Jeonju 
3 Jung DY, Ha HK, Kim AN, Lee SM (2000) Cytotoxicity of SD-994 from Artemisia argyi against L1210 cells with concomitant induction of antioxidant enzymes. YAKHAK HOEJI 44: 213-233. 
4 Jung DY, Park SW (2002) Cytotoxicity of water fraction of Artemisia argyi against L1210 cells and antioxidant enzyme activities. J Appl Pharmacol 46: 39-46. 
5 Choi BB, Lee HJ, Bang SK (2005) Studies on the volatile flavor components and biochemical characterizations of Artemisia princeps and A. argyi. J Korean Soc Food Sci Nutr 18: 334-340. 
6 Park WS (2009) Effect of water extract from Artemisiae argyi folium on mouse macrophage stimulated by LPS. Korean J Herbol 24: 151-157. 
7 Zhang, Xue MQ, Bai YC, Yuan HH, Zhao HL, Lan MB (2012) 3,5-Dicaffeoylquinic acid isolated from Artemisia argyi and its ester derivatives exert anti-Leucyl-tRNA synthetase of Giardia lamblia (GlLeuRS) and potential anti-giardial effects. Fitoterapia 83: 1281-1285. doi: 10.1016/j.fitote.2012.05.016    DOI
8 Aneta G, Malgorzata MD (2014) Lycopene-antioxidant with radioprotective and anticancer properties. A review. Rocz Panstw Zakl Hig 65(4): 263-271 
9 Jung EB, Shin MK (1990) In Hyang Yak DaeSaJun. 3rd edition. Young Lim Sa: Seoul, Korea 
10 Wu G, Fang YZ, Yang S, Lupton RJ, Turner DN (2004) Glutathione metabolism and its implications for health. J Nutr 134(3): 489-492. doi: 10.1093/jn/134.3.489    DOI
11 Mailloux RJ, McBride SL, Harper ME (2013) Unearthing the secrets of mitochondrial ROS and glutathione in bioenergetics. Trends Biochem Sci 38(12): 592-602. doi: 10.1016/j.tibs.2013.09.001    DOI
12 Yang SL, Yu PL, Chung KR (2016) The glutathione peroxidase-mediated reactive oxygen species resistance, fungicide sensitivity and cell wall construction in the citrus fungal pathogen Alternaria alternata. Environ Microbiol 18(3): 923-935. doi: 10.1111/1462-2920.13125    DOI
13 Kim HG, Jung YS, Oh SM, Oh HJ, Ko JH, Kim DO, Kang SC, Lee YG, Lee DY, Baek NI (2020) Coreolanceolins A-E, new flavanones from the flowers of Coreopsis lanceolata and their antioxidative and anti-inflammatory effects. Antioxidants 9: 539-555. doi: 10.3390/antiox9060539    DOI
14 Jeon HJ, Kim HG (2021) Flavone from the Lycopersicon esculentum and their antioxidant capacity through GSH recovery effect. J Appl Biol Chem 64: 363-368. doi: 10.3839/jabc.2021.049    DOI
15 Kim JY, Kim HJ, Park KS, Lee YY, Lim YH, Lim YH (2005) Synthesis of naringenin amino acid esters as potential CDK2 inhibitors. Bull Korean Chem Soc 26(12): 2065-2068.    DOI
16 Kim HG, Nam YH, Jung YS, Oh SM, Trong Nguyen N, Lee MH, Kim DO, Kang TH, Lee DY, Baek NI (2021) Aurones and flavonols from Coreopsis lanceolata L. flowers and their anti-oxidant, pro-inflammatory inhibition effects, and recovery effects on alloxan-induced pancreatic islets in zebrafish. Molecules 26: 6098-6113. doi: 10.3390/molecules26206098   DOI
17 Park CH, Kim KH, Lee IK, Lee SY, Choi SU, Lee JH, Lee KR (2011) Phenolic constituents of Acorus gramineus. Arch Pharm Res 34(8): 1289-1296. doi: 10.1007/s12272-011-0808-6    DOI
18 Al-Dabbas, MM, Kitahara K, Suganuma T, Hashimoto F, Tadera K (2006) Antioxidant and α-amylase inhibitory compounds from aerial parts of Varthemia iphionoides Boiss. Biosci Biotechnol Biochem 70(9): 2178-2184. doi: 10.1271/bbb.60132    DOI
19 Daskiewicz JB, Depeint F, Viornery L, Bayet C, Comte-Sarrazin G, Comte G, Gee JM, Johnson IT, Ndjoko K, Hostettmann K, Barron D (2005) Effects of flavonoids on cell proliferation and caspase activation in a human colonic cell line HT29: An SAR study. J Med Chem 48(8): 2790-2804. doi: 10.1021/jm040770b    DOI