The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology (한방안이비인후피부과학회지)

The Society of Korean Medicine Ophthalmology, Otolaryngology & Dermatology (대한한방안이비인후피부과학회)
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Anti-inflammatory Effects of Cheongsimyanggyeok-san via NF-𝜅B Inhibition

NF-𝜅B억제를 통한 청심양격산(淸心凉膈散)의 항염증 효과

  • Kim, Nan-Ee (Dep. of Oriental Ophthalmology and Otolaryngology and Dermatology, Daegu Hanny University) ;
  • Kim, Yeon-Soo (Dep. of Oriental Ophthalmology and Otolaryngology and Dermatology, Daegu Hanny University) ;
  • Jee, Seon-Young (Dep. of Oriental Ophthalmology and Otolaryngology and Dermatology, Daegu Hanny University) ;
  • Hwangbo, Min (Dep. of Oriental Ophthalmology and Otolaryngology and Dermatology, Daegu Hanny University)
  • 김난이 (대구한의대학교 부속 대구한방병원 안이비인후피부과) ;
  • 김연수 (대구한의대학교 부속 대구한방병원 안이비인후피부과) ;
  • 지선영 (대구한의대학교 부속 포항한방병원 안이비인후피부과) ;
  • 황보민 (대구한의대학교 부속 대구한방병원 안이비인후피부과)
  • Received : 2019.04.04
  • Accepted : 2019.04.29
  • Published : 2019.05.25
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Abstract

Objectives : The purpose of this study is to investigate the anti-inflammatory effect of Cheongsimyanggyeoksan(CYS) water extract in vitro and in vivo. Methods : To evaluate the anti-inflammatory effect of CYS, Raw 264.7 cells were pretreated with $3-300{\mu}g/m{\ell}$ of CYS for 1h, and then exposed to $1{\mu}g/m{\ell}$ of LPS. The cell viability was detected by MTT assay. Productions of nitric oxide(NO) and pro-inflammatory cytokines were measured in culture media. Protein levels of inducible nitric oxide synthase(iNOS) and Nuclear factor-${\kappa}$B($NF-{\kappa}B$) were determined by immunoblot analysis. The effect of CYS on acute inflammation in vivo was evaluated thorugh measurment of carrageenan-induced paw edema. Results : In vitro study, cell viability assay CYS treatment of $3-300{\mu}g/m{\ell}$ has no cytotoxicity in Raw 264.7 cells. LPS-induced NO production was significantly inhibited by pretreatment with $30-300{\mu}g/m{\ell}$ of CYS. Production of interleukin-6, -$1{\beta}$ and tumor necrosis factor-${\alpha}$ by LPS were significantly decreased by CYS pretreatment. CYS reduced LPS-mediated iNOS expression. Moreover, CYS significantly induced $I-{\kappa}B{\alpha}$ expression and reduced $NF-{\kappa}B$ expression. In vivo study, CYS significantly reduced the increases of paw swelling. Conclusions : These results suggest the clinical basis of CYS for the treatment of inflammatory diseases.

Keywords

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Fig. 1. Effect of CYS on Cell Viability in Raw 264.7 Cells

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Fig. 2. Effect of CYS on NO Production and Cell Viability in LPS-Stimulated Raw 264.7 Cells

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Fig. 3. Effect of CYS on NO Production and Cell Viability in PGN-Stimulated in Raw 264.7 Cells

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Fig. 4. Effect of CYS on iNOS in LPS-Stimulated Raw 264.7 Cells

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Fig. 5. Effect of CYS on Cytokine Production in LPS-Stimulated Raw 264.7 Cells

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Fig. 6. Effect of CYS on NF-κB Signaling Pathway in LPS-Stimulated Raw 264.7 Cells

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Fig. 7. Effect of CYS on CA-Induced Paw Edema

Table 1. The Composition and Amount of Cheongsimyanggyeok-san

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