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http://dx.doi.org/10.6114/jkood.2019.32.2.011

Anti-inflammatory Effects of Cheongsimyanggyeok-san via NF-𝜅B Inhibition  

Kim, Nan-Ee (Dep. of Oriental Ophthalmology and Otolaryngology and Dermatology, Daegu Hanny University)
Kim, Yeon-Soo (Dep. of Oriental Ophthalmology and Otolaryngology and Dermatology, Daegu Hanny University)
Jee, Seon-Young (Dep. of Oriental Ophthalmology and Otolaryngology and Dermatology, Daegu Hanny University)
Hwangbo, Min (Dep. of Oriental Ophthalmology and Otolaryngology and Dermatology, Daegu Hanny University)
Publication Information
The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology / v.32, no.2, 2019 , pp. 11-23 More about this Journal
Abstract
Objectives : The purpose of this study is to investigate the anti-inflammatory effect of Cheongsimyanggyeoksan(CYS) water extract in vitro and in vivo. Methods : To evaluate the anti-inflammatory effect of CYS, Raw 264.7 cells were pretreated with $3-300{\mu}g/m{\ell}$ of CYS for 1h, and then exposed to $1{\mu}g/m{\ell}$ of LPS. The cell viability was detected by MTT assay. Productions of nitric oxide(NO) and pro-inflammatory cytokines were measured in culture media. Protein levels of inducible nitric oxide synthase(iNOS) and Nuclear factor-${\kappa}$B($NF-{\kappa}B$) were determined by immunoblot analysis. The effect of CYS on acute inflammation in vivo was evaluated thorugh measurment of carrageenan-induced paw edema. Results : In vitro study, cell viability assay CYS treatment of $3-300{\mu}g/m{\ell}$ has no cytotoxicity in Raw 264.7 cells. LPS-induced NO production was significantly inhibited by pretreatment with $30-300{\mu}g/m{\ell}$ of CYS. Production of interleukin-6, -$1{\beta}$ and tumor necrosis factor-${\alpha}$ by LPS were significantly decreased by CYS pretreatment. CYS reduced LPS-mediated iNOS expression. Moreover, CYS significantly induced $I-{\kappa}B{\alpha}$ expression and reduced $NF-{\kappa}B$ expression. In vivo study, CYS significantly reduced the increases of paw swelling. Conclusions : These results suggest the clinical basis of CYS for the treatment of inflammatory diseases.
Keywords
Anti-inflammation; nitric oxide(NO); Cheongsimyanggyeok-san(CYS);
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