The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology (한방안이비인후피부과학회지)

The Society of Korean Medicine Ophthalmology, Otolaryngology & Dermatology (대한한방안이비인후피부과학회)
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Inhibitory Effects of KM1701 on Airway Cell Infiltration in OVA-Induced Mouse Model

OVA-유도 쥐 모델에서 기도 세포 침윤에 대한 KM1701의 억제효과

  • Received : 2019.04.03
  • Accepted : 2019.04.27
  • Published : 2019.05.25
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Abstract

Objectives : The objective of present study is to evaluate anti-inflammatory and anti-allergic effects of Perilla(Perilla frutescens; Labiatae, PF), the roots of Peucedanum praeruptorum(PP) and the root of Scutellaria baicalensis(SB) in vitro and anti-asthmatic effects of mixture of PF, PP and SB(PS) on ovalbumin (OVA)-induced asthma in BALB/c mice. Methods : Anti-inflammatory and anti-allergic effects were observed on the lipopolysaccharide(LPS) treated RAW 264.7 cells through Nitric Oxide(NO) production and RBL-2H3 cells through ${\beta}$-hexosaminidase. Anti-asthmatic effects were observed on the number of inflammatory cells in bronchoalveolar lavage fluid(BALF) and the level of IgE in serum on OVA-induced BALB/c mice. Results : The treatment of PF, PP and SB(12.5, 25, 50, $100{\mu}g/m{\ell}$) resulted in a significant inhibition of NO production in RAW 264.7 cells and mast cell degranulation in RBL-2H3 cells. Oral administration of PS(400mg/kg/day) resulted in a significant reduction in the numbers of eosinophils in BALF and level of IgE in serum. Conclusion : The oral administration of PS is effective in ameliorating the eosinophilic infiltration in vivo and thus can be a good therapeutic candidate for allergic asthma.

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OOBHC1_2019_v32n2_1_f0002.png 이미지

Fig. 2. Effects of PS1-5 on BAL Fluid Cell Infiltration and IgE Levels of OVA-Challenged Mice

OOBHC1_2019_v32n2_1_f0003.png 이미지

Fig. 3. Effects of KM1701 on Release of β-hexosaminidase from IgE-Sensitized RBL-2H3 Cells and BAL Fluid Cell Infiltration of OVA-Challenged Mice

OOBHC1_2019_v32n2_1_f0004.png 이미지

Fig. 1. Inhibitory Effects of PF, PP and SB on Release of NO Production from LPS-Induced RAW 264.7 Cells and β-hexosaminidase from IgE-Sensitized RBL-2H3 Cells

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