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http://dx.doi.org/10.6114/jkood.2019.32.2.001

Inhibitory Effects of KM1701 on Airway Cell Infiltration in OVA-Induced Mouse Model  

Lim, Soon-Min (Kolmar, Korea Co. Ltd.)
Choi, Han-Seok (Kolmar, Korea Co. Ltd.)
Kim, Sang-Back (Kolmar, Korea Co. Ltd.)
Kim, Ye-Jin (Kolmar, Korea Co. Ltd.)
Kang, Ki-Sung (College of Korean Medicine, Gachon University)
Shin, Myoung-Sook (College of Korean Medicine, Gachon University)
Kim, Kyung-Jun (College of Korean Medicine, Gachon University)
Hwang, Gwi-Seo (College of Korean Medicine, Gachon University)
Koo, Bon-Am (Kolmar, Korea Co. Ltd.)
Publication Information
The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology / v.32, no.2, 2019 , pp. 1-10 More about this Journal
Abstract
Objectives : The objective of present study is to evaluate anti-inflammatory and anti-allergic effects of Perilla(Perilla frutescens; Labiatae, PF), the roots of Peucedanum praeruptorum(PP) and the root of Scutellaria baicalensis(SB) in vitro and anti-asthmatic effects of mixture of PF, PP and SB(PS) on ovalbumin (OVA)-induced asthma in BALB/c mice. Methods : Anti-inflammatory and anti-allergic effects were observed on the lipopolysaccharide(LPS) treated RAW 264.7 cells through Nitric Oxide(NO) production and RBL-2H3 cells through ${\beta}$-hexosaminidase. Anti-asthmatic effects were observed on the number of inflammatory cells in bronchoalveolar lavage fluid(BALF) and the level of IgE in serum on OVA-induced BALB/c mice. Results : The treatment of PF, PP and SB(12.5, 25, 50, $100{\mu}g/m{\ell}$) resulted in a significant inhibition of NO production in RAW 264.7 cells and mast cell degranulation in RBL-2H3 cells. Oral administration of PS(400mg/kg/day) resulted in a significant reduction in the numbers of eosinophils in BALF and level of IgE in serum. Conclusion : The oral administration of PS is effective in ameliorating the eosinophilic infiltration in vivo and thus can be a good therapeutic candidate for allergic asthma.
Keywords
KM1701; Airway Cell Infiltration; OVA-induced Mouse Model;
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