Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
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Occurrence and eradication of Plum pox virus on Ornamentals in Korea, 2016-2017

2016-2017년 국내 핵과류에서의 자두곰보병 발생 및 방제

  • Kim, Mikyeong (Crop Protection Division, National Institute of Agricultural Science) ;
  • Kim, Gi-Su (Department of Central Area Crop Science, National Institute of Crop Science) ;
  • Kwak, Hae-Ryun (Crop Protection Division, National Institute of Agricultural Science) ;
  • Kim, Jeong-Eun (Crop Protection Division, National Institute of Agricultural Science) ;
  • Seo, Jang-Kyun (Graduate School of International Agricultural Technology, Seoul National University) ;
  • Hong, Seong-Jun (Extension Service Bureau, Rural Development Administration) ;
  • Lee, Gyeong-Jae (Extension Service Bureau, Rural Development Administration) ;
  • Kim, Ju-Hui (Jeollabuk-do Agricultural Research and Extension Services) ;
  • Choi, Min-Kyeong (Jeollabuk-do Agricultural Research and Extension Services) ;
  • Kim, Byeong-Ryeon (Chungcheongnam-do Agricultural Research and Extension Services) ;
  • Kim, Ji-Gwang (Chungcheongnam-do Agricultural Research and Extension Services) ;
  • Han, In-Yeong (Gyeongsangnam-do Agricultural Reasarch and Extension Services) ;
  • Lee, Hyeon-Ju (Gyeonggi-do Agricultural Research and Extension Services) ;
  • Won, Heon-Seop (Gangwon-do Agricultural Research and Extension Services) ;
  • Kang, Hyo-Jung (Chungcheongbuk-do Agricultural Research and Extension Services) ;
  • Han, Jong-Woo (Chungcheongbuk-do Agricultural Research and Extension Services) ;
  • Ko, Suk-Ju (Jeollanam-do Agricultural Research and Extension Services) ;
  • Kim, Hyo-Jeong (Jeollanam-do Agricultural Research and Extension Services) ;
  • Kim, Seung-Han (Gyeongsangbuk-do Agricultural Research and Extension Services) ;
  • Lee, Jung-Hywan (Gyeongsangbuk-do Agricultural Research and Extension Services) ;
  • Choi, Hong-Soo (Crop Protection Division, National Institute of Agricultural Science)
  • Received : 2018.11.17
  • Accepted : 2019.03.11
  • Published : 2019.03.31
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Abstract

Plum pox virus (PPV) is a significant viral disease in Prunus spp. worldwide. A nationwide survey was started in Prunus spp. orchards, since PPV was first detected from peach in Korea, 2015. During 2016-2017, samples were collected from 30,333 trees in 1,985 orchards of stone fruits in 8 provinces and 4 cities, Korea and tested by RT-PCR using specific PPV primer set. As a result, 21 trees including peach (9 trees), Japanese apricot (4 trees), plum (1 tree), apricot (7 trees) in 10 orchards were infected and controlled by eradication program. Amplicons of the expected size (547 bp) were obtained from total RNA of seven peach trees in 2016, and directly sequenced. BLAST analysis revealed the highest nucleotide (NT) identity (99%) with a PPV D isolates (LC331298, LT600782) in Genbank. The seven isolates from shared nt sequence identities of 98 to 100% with one another. Phylogenetic analysis showed the isolates in peach clustered closely with the PPV-D isolates from Korea, Japan, USA, and Canada. This is, to our knowledge, the first report of the presence of PPV in Prunus spp. orchards in Korea, 2016-2017, we hope that our results and efforts will contribute to effective measures for eradication of PPV.

전 세계적으로 자두곰보바이러스는 핵과류에 발생하는 중요한 바이러스이다. 2015년 국내 복숭아에서 자두곰보바이러스 감염이 확인된 이후 국내 핵과류 과원에 대한 자두곰보바이러스 발생 조사가 시작되었다. 2016-2017년 국내 핵과류 1,985과원의 30,333주 나무에서 시료를 채집하여 특이 프라이머를 이용한 RT-PCR 검정 결과, 10과원의 21주 나무에서 자두곰보바이러스가 확인되었고, 박멸조치에 따라 감염 주에 대한 폐기 등 방제가 실시되었다. 2016년 자두곰보바이러스에 감염된 복숭아 나무 7주의 total RNA에서 PCR 산물 약 547 bp을 얻은 후 direct sequencing 하여 염기서열을 결정하였다. BLAST 검색 결과 Genbank에 등록된 자두곰보바이러스 D 계통 분리 주들(LC331298, LT600782)과 99% 높은 염기서열 상동성을 보였으며, 국내 복숭아 나무 7개 분리 주 간에 98-100% 염기서열 상동성을 보였다. Phylogenetic tree 분석 결과 한국, 일본, 미국, 캐나다에서 보고된 D 계통과 높은 유연관계를 가지는 것을 확인 할 수 있었다. 이 보고는 2016-2017년 국내 핵과류 과원의 자두곰보바이러스 발생 현황에 대한 첫 보고이며, 이를 바탕으로 발생 주 제거 등 국내 금지급 자두곰보병의 예방 및 방제 대책 수립을 위한 기초자료로 활용하고자 하였다.

Keywords

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Fig. 1. Map of Plum pox virus (PPV) incidence in South Korea, 2016- 2017. Dots (area, host, no of tree) indicates occurrence of the PPV characterized in this study. CG, Chungcheongnam-do geumsangun; JG, Jeollabuk-do gunsan-si; JB, Jeollabuk-do buan-gun; GC, Gyeongsangbuk-do cheongdo-gun; GJ, Gyeongsangnam-do Jinju- si.

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Fig. 2. Phylogenetic analysis by Maximum likelihood (ML) method showing the relationship of Plum pox virus (PPV) isolates from countries based on an alignment of nucleotide sequences. ML phylogeny reconstructed using simultaneous NNI moves. The log-likelihood of the corresponding phylogenetic model is -40594.32868. Numbers in the tree correspond to non-parametric bootstrap supports (100) replicates.

Table 1. Sample surveys conducted in Prunus spp. orchard in Korea for Plum pox virus, 2016

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Table 2. Sample surveys conducted in Prunus spp. orchard in Korea for Plum pox virus, 2017

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Table 3. Incidence of Plum pox virus in Prunus spp. in Korea, 2016 - 2017

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