Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
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Difference of Gray Mold Severity at Roses Caused by Botrytis cinerea Strains

잿빛곰팡이병원균 Botrytis cinerea 균주 종류별 장미 발병 정도의 차이

  • Hwang, Kyu-Hyon (Division of Horticulture Research, Gyeonggido Agricultural Research & Extension Services) ;
  • Hong, Seung-Min (Division of Horticulture Research, Gyeonggido Agricultural Research & Extension Services) ;
  • Lee, Young-Soon (Mushroom Research Institute, Gyeonggido Agricultural Research & Extension Services) ;
  • Lee, Hyun-Ju (Division of Environmental Agricultural Research, Gyeonggido Agricultural Research & Extension Services) ;
  • Seo, Myeong-Whoon (Division of Horticulture Research, Gyeonggido Agricultural Research & Extension Services)
  • 황규현 (경기도농업기술원 원예연구과) ;
  • 홍승민 (경기도농업기술원 원예연구과) ;
  • 이영순 (경기도농업기술원 버섯연구소) ;
  • 이현주 (경기도농업기술원 환경농업연구과) ;
  • 서명훈 (경기도농업기술원 원예연구과)
  • Received : 2019.01.12
  • Accepted : 2019.03.26
  • Published : 2019.03.31
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Abstract

Botrytis cinerea is the pathogen for a gray mold generating problems during the cultivation and transportation of roses. But there is little information about the difference of the symptom severity caused by gray mold on rose varieties and pathogen strains. 16 strains were collected from the rose cultivation area to confirm the degree of disease occurrence against strains and each variety. Collected 16 strains were identified based on the sequences analysis of ITS region of ribosomal DNA by using specific primers. The sequence analysis was performed by comparing the sequences to find a difference. To confirm the difference in disease occurrence for each strains, the difference was classified from 0 to 5 stages using charmant variety as a control. The data was confirmed through Kruskal-Wallis ANOVA. The result showed the significant difference in the pathogenicity caused by strains. WNG6_5 showed the lowest pathogenicity with 0.24 and WNG6_3 showed the highest with 3.20. The difference between two strains were almost 3.0. In addition, nine varieties of roses were more investigated with three strains such as the strains of WNG6_5, Hwa_1, and WNG6_3. The result showed that the Love Letter variety showed resistance and the Ice Bear variety was sensitive to three strains. Taken together, this study showed the significant difference by the interactions of rose varieties and gray mold strains.

잿빛곰팡이병은 Botrytis cinerea가 원인균으로 알려져 있으며 장미 재배와 유통 시에 문제가 되고 있다. 그러나 병원균 균주와 장미 품종에 따른 발병정도의 차이를 연구한 결과가 없어 이를 구명하고자 하였다. 장미 재배 지역에서 분리한 균주를 수집하고 병원 균주별 장미 품종별 발병정도를 확인한 결과는 다음과 같다. 서울시립대에서 11종, 국립원예특작과학원에서 3종, 경기도농업기술원 장미재배온실에서 2종을 분리하였으며, 분리한 균주를 ribosomal DNA의 ITS region에 특이적인 primer를 사용하여 동정하였고, sequencing을 통해 염기서열을 비교한 결과 큰 차이가 없음을 확인하였다. 잿빛곰팡이 균주별 발병정도의 차이를 확인하기 위해 장미 품종인 샤만트 품종에 대하여 Botrytis cinerea 균주별 발병정도의 차이를 0-5의 단계로 나누어 조사하고, Kruskal-Wallis ANOVA를 통해 분석한 결과, 균주에 따라 병원성이 유의적으로 차이를 나타냈다. WNG6_5의 경우 0.24 로 병원성이 가장 낮았으며, WNG6_3의 경우 3.20로 병원성이 가장 높아, 균주별 병원성의 차이는 거의 3.0 정도의 차이를 보였다. 장미 9품종에 대한 발병정도의 차이를 확인하기 위해 균주 WNG6_5, Hwa_1, WNG6_3을 선발하여 분석한 결과 러브레터 품종이 3가지 균주에 대해 저항성을 보였으며, 아이스베어 품종이 감수성을 나타내었다. 본 연구를 통해 장미 품종 및 병원균의 종류에 따라 발병정도의 유의적인 차이와 교호작용의 효과가 존재함을 확인할 수 있었다.

Keywords

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Fig. 1. Morphological characteristics of collected Botrytis cinerea strains.

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Fig. 2. Multiple alignment result of collected Botrytis cinerea strains.

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Fig. 3. Boxplot by accession A) and Strain B), Interaction effect plot of Strain C) and Accession D).

Table 1. Information of collected Botrytis Cinerea strains

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Table 2. Difference of severity by Botrytis cinerea strains

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Table 3. Result of Aligned Rank Transform(ART) ANOVA table factored by rose accession and Botrytis cinerea strains

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