Mycobiology

The Korean Society of Mycology (한국균학회)
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Depletion of ${\varepsilon}$-COP in the COPI Vesicular Coat Reduces Cleistothecium Production in Aspergillus nidulans

  • Kang, Eun-Hye (Department of Microbiology and Molecular Biology, College of Bioscience and Biotechnology, Chungnam National University) ;
  • Song, Eun-Jung (Department of Microbiology and Molecular Biology, College of Bioscience and Biotechnology, Chungnam National University) ;
  • Kook, Jun Ho (Department of Microbiology and Molecular Biology, College of Bioscience and Biotechnology, Chungnam National University) ;
  • Lee, Hwan-Hee (Department of Microbiology and Molecular Biology, College of Bioscience and Biotechnology, Chungnam National University) ;
  • Jeong, Bo-Ri (Department of Microbiology and Molecular Biology, College of Bioscience and Biotechnology, Chungnam National University) ;
  • Park, Hee-Moon (Department of Microbiology and Molecular Biology, College of Bioscience and Biotechnology, Chungnam National University)
  • Received : 2014.10.22
  • Accepted : 2015.01.05
  • Published : 2015.03.31
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Abstract

We have previously isolated ${\varepsilon}$-COP, the ${\alpha}$-COP interactor in COPI of Aspergillus nidulans, by yeast two-hybrid screening. To understand the function of ${\varepsilon}$-COP, the $aneA^+$ gene for ${\varepsilon}$-COP/AneA was deleted by homologous recombination using a gene-specific disruption cassette. Deletion of the ${\varepsilon}$-COP gene showed no detectable changes in vegetative growth or asexual development, but resulted in decrease in the production of the fruiting body, cleistothecium, under conditions favorable for sexual development. Unlike in the budding yeast Saccharomyces cerevisiae, in A. nidulans, over-expression of ${\varepsilon}$-COP did not rescue the thermo-sensitive growth defect of the ${\alpha}$-COP mutant at $42^{\circ}C$. Together, these data show that ${\varepsilon}$-COP is not essential for viability, but it plays a role in fruiting body formation in A. nidulans.

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References

  1. Popoff V, Adolf F, Brugger B, Wieland F. COPI budding within the Golgi stack. Cold Spring Harb Perspect Biol 2011;3:a005231.
  2. Kirchhausen T. Three ways to make a vesicle. Nat Rev Mol Cell Biol 2000;1:187-98. https://doi.org/10.1038/35043117
  3. Pellett PA, Dietrich F, Bewersdorf J, Rothman JE, Lavieu G. Inter-Golgi transport mediated by COPI-containing vesicles carrying small cargoes. Elife 2013;2:e01296.
  4. Duden R, Kajikawa L, Wuestehube L, Schekman R. ${\varepsilon}$-COP is a structural component of coatomer that functions to stabilize ${\alpha}$-COP. EMBO J 1998;17:985-95. https://doi.org/10.1093/emboj/17.4.985
  5. Cosson P, Demolliere C, Hennecke S, Duden R, Letourneur F. ${\delta}$- and ${\zeta}$-COP, two coatomer subunits homologous to clathrin-associated proteins, are involved in ER retrieval. EMBO J 1996;15:1792-8.
  6. Cosson P, Letourneur F. Coatomer interaction with di-lysine endoplasmic reticulum retention motifs. Science 1994;263:1629-31. https://doi.org/10.1126/science.8128252
  7. Letourneur F, Gaynor EC, Hennecke S, Demolliere C, Duden R, Emr SD, Riezman H, Cosson P. Coatomer is essential for retrieval of dilysine-tagged proteins to the endoplasmic reticulum. Cell 1994;79:1199-207. https://doi.org/10.1016/0092-8674(94)90011-6
  8. Kim KH, Park HM. Enhanced secretion of cell wall bound enolase into culture medium by the soo1-1 mutation of Saccharomyces cerevisiae. J Microbiol 2004;42:248-52.
  9. Lee DW, Ahn GW, Kang HG, Park HM. Identification of a gene, SOO1, which complements osmo-sensitivity and defect in in vitro ${\beta}$-1,3-glucan synthase activity in Saccharomyces cerevisiae. Biochim Biophys Acta 1999;1450:145-54. https://doi.org/10.1016/S0167-4889(99)00041-5
  10. Lee DW, Kim KH, Chun SC, Park HM. Characterization of cell wall proteins from the soo1-1/ret1-1 mutant of Saccharomyces cerevisiae. J Microbiol 2002;40:219-23.
  11. Kim KH, Kim EK, Kim SJ, Park YH, Park HM. Effect of Saccharomyces cerevisiae ret1-1 mutation on glycosylation and localization of the secretome. Mol Cells 2011;31:151-8.
  12. Kim KH, Kim EK, Jeong KY, Park YH, Park HM. Effects of mutations in the WD40 domain of ${\alpha}$-COP on its interaction with the COPI coatomer in Saccharomyces cerevisiae. J Microbiol 2012;50:256-62. https://doi.org/10.1007/s12275-012-1326-z
  13. Kozubowski L, Thompson JW, Cardenas ME, Moseley MA, Heitman J. Association of calcineurin with the COPI protein Sec28 and the COPII protein Sec13 revealed by quantitative proteomics. PLoS One 2011;6:e25280. https://doi.org/10.1371/journal.pone.0025280
  14. Whittaker SL, Lunness P, Milward KJ, Doonan JH, Assinder SJ. $sod^{VI}C$ is an ${\alpha}$-COP-related gene which is essential for establishing and maintaining polarized growth in Aspergillus nidulans. Fungal Genet Biol 1999;26:236-52. https://doi.org/10.1006/fgbi.1999.1117
  15. Lee HH, Park JS, Chae SK, Maeng PJ, Park HM. Aspergillus nidulans $sod^{VI}C1$ mutation causes defects in cell wall biogenesis and protein secretion. FEMS Microbiol Lett 2002;208:253-7. https://doi.org/10.1111/j.1574-6968.2002.tb11090.x
  16. Song EJ, Kim KH, Lee HH, Park JS, Kang EH, Park HM. Analysis of protein domain for interaction between ${\alpha}$-COP and ${\varepsilon}$-COP in Aspergillus nidulans. Kor J Mycol 2012;40:224-8. https://doi.org/10.4489/KJM.2012.40.4.224
  17. Kang EH, Kim JA, Oh HW, Park HM. LAMMER kinase LkhA plays multiple roles in the vegetative growth and asexual and sexual development of Aspergillus nidulans. PLoS One 2013;8:e58762. https://doi.org/10.1371/journal.pone.0058762
  18. Lee JY, Kim LH, Kim HE, Park JS, Han KH, Han DM. A putative APSES transcription factor is necessary for normal growth and development of Aspergillus nidulans. J Microbiol 2013;51:800-6. https://doi.org/10.1007/s12275-013-3100-2
  19. Yu JH, Hamari Z, Han KH, Seo JA, Reyes-Dominguez Y, Scazzocchio C. Double-joint PCR: a PCR-based molecular tool for gene manipulations in filamentous fungi. Fungal Genet Biol 2004;41:973-81. https://doi.org/10.1016/j.fgb.2004.08.001
  20. Upshall A, Mortimore ID. Isolation of aneuploid-generating mutants of Aspergillus nidulans, one of which is defective in interphase of the cell cycle. Genetics 1984;108:107-21.
  21. Kitazawa D, Yamaguchi M, Mori H, Inoue YH. COPImediated membrane trafficking is required for cytokinesis in Drosophila male meiotic divisions. J Cell Sci 2012;125(Pt 15):3649-60. https://doi.org/10.1242/jcs.103317
  22. Han KH, Lee DB, Kim JH, Kim MS, Han KY, Kim WS, Park YS, Kim HB, Han DM. Environmental factors affecting development of Aspergillus nidulans. J Microbiol 2003;41:34-40.
  23. Lin YP, Chen LR, Chen CF, Liou JF, Chen YL, Yang JR, Shiue YL. Identification of early transcripts related to male development in chicken embryos. Theriogenology 2010;74:1161-78. https://doi.org/10.1016/j.theriogenology.2010.05.017
  24. Claerhout S, Dutta B, Bossuyt W, Zhang F, Nguyen-Charles C, Dennison JB, Yu Q, Yu S, Balazsi G, Lu Y, et al. Abortive autophagy induces endoplasmic reticulum stress and cell death in cancer cells. PLoS One 2012;7:e39400. https://doi.org/10.1371/journal.pone.0039400
  25. Todd AG, Lin H, Ebert AD, Liu Y, Androphy EJ. COPI transport complexes bind to specific RNAs in neuronal cells. Hum Mol Genet 2013;22:729-36. https://doi.org/10.1093/hmg/dds480
  26. Custer SK, Todd AG, Singh NN, Androphy EJ. Dilysine motifs in exon 2b of SMN protein mediate binding to the COPI vesicle protein ${\alpha}$-COP and neurite outgrowth in a cell culture model of spinal muscular atrophy. Hum Mol Genet 2013;22:4043-52. https://doi.org/10.1093/hmg/ddt254