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http://dx.doi.org/10.5941/MYCO.2015.43.1.31

Depletion of ${\varepsilon}$-COP in the COPI Vesicular Coat Reduces Cleistothecium Production in Aspergillus nidulans  

Kang, Eun-Hye (Department of Microbiology and Molecular Biology, College of Bioscience and Biotechnology, Chungnam National University)
Song, Eun-Jung (Department of Microbiology and Molecular Biology, College of Bioscience and Biotechnology, Chungnam National University)
Kook, Jun Ho (Department of Microbiology and Molecular Biology, College of Bioscience and Biotechnology, Chungnam National University)
Lee, Hwan-Hee (Department of Microbiology and Molecular Biology, College of Bioscience and Biotechnology, Chungnam National University)
Jeong, Bo-Ri (Department of Microbiology and Molecular Biology, College of Bioscience and Biotechnology, Chungnam National University)
Park, Hee-Moon (Department of Microbiology and Molecular Biology, College of Bioscience and Biotechnology, Chungnam National University)
Publication Information
Mycobiology / v.43, no.1, 2015 , pp. 31-36 More about this Journal
Abstract
We have previously isolated ${\varepsilon}$-COP, the ${\alpha}$-COP interactor in COPI of Aspergillus nidulans, by yeast two-hybrid screening. To understand the function of ${\varepsilon}$-COP, the $aneA^+$ gene for ${\varepsilon}$-COP/AneA was deleted by homologous recombination using a gene-specific disruption cassette. Deletion of the ${\varepsilon}$-COP gene showed no detectable changes in vegetative growth or asexual development, but resulted in decrease in the production of the fruiting body, cleistothecium, under conditions favorable for sexual development. Unlike in the budding yeast Saccharomyces cerevisiae, in A. nidulans, over-expression of ${\varepsilon}$-COP did not rescue the thermo-sensitive growth defect of the ${\alpha}$-COP mutant at $42^{\circ}C$. Together, these data show that ${\varepsilon}$-COP is not essential for viability, but it plays a role in fruiting body formation in A. nidulans.
Keywords
${\alpha}$-COP; Aspergillus nidulans; Cleistothecium; ${\varepsilon}$-COP; Sexual development;
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